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AIM To establish the quality standard for Weigela japonica Thunb.var.sinica (Rehd.) Bailey (W.j.).METHODS TLC was adopted in this medicinal material's qualitative identification after morphological identification and microscopic identification.The contents of water,total ash,acid-insoluble ash and extracts were detected according to Chinese Pharmacopoeia methods.Then the contents of scopoletin and total coumarins were determined by HPLC and UV,respectively.RESULTS The morphologies and microscopic characters of W.j.could be distinguished from other same generic plants.The clear TLC spot displayed good resolution.The contents of water,total ash,acid-insoluble ash,water-soluble extract and acid-soluble extract were no more than 12.0%,no more than 2.0%,no more than 0.5%,no less than 5.0% and no less than 4.5%,respectively.Scopoletin and total coumarins showed good linear relationships within the ranges of 1.25-40.0 μg/mL(r =0.999 7)and 2.0-64.0 μg/mL (r =0.999 9),whose average recoveries were 98.19% (RSD =0.90%) and 99.21% (RSD =2.5%),respectively.CONCLUSION This accurate and reliable method can be used for the quality control of W.j..
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Objective: To observe the effects of liraglutide on angiogenesis and myocardium protection in acute myocardial infarction (AMI) rats with its mechanisms. Methods: Rat's AMI model was established by left anterior descending of coronary ligation. AMI rats were randomly divided into 3 groups: Control group, the rats received subcutaneous injection of normal saline, Low dose (LS) group and High dose (HS) group, rats received subcutaneous injection of liraglutide 70μg/(kg?d) and 140μg/(kg?d) respectively; in addition, Sham operation group, rats received normal saline. n=6 in each group, all animals were treated for 2 weeks. 4 weeks later, cardiac structure and function were assessed by echocardiography, morphological changes of myocardium were observed by HE staining, collagen volume fraction (CVF) was calculated by Masson staining, myocardial microvessel density (MVD) and protein expression of vascular endothelial growth factor (VEGF) in marginal zone of infracted region were detected by immunohistochemistry, VEGF protein level was examined by Western blot analysis. Results: Compared with Sham operation group, Control group showed decreased LVEF, LVFS and increased LVEDd, LVESd, CVF, all P<0.01; while MVD and VEGF protein level were similar between 2 groups, P>0.05. Compared with Control group, LS group and HS group had obviously increased LVEF, LVFS, P<0.01 and decreased LVEDd, LVESd, P<0.05, obviously decreased CVF, P<0.01; obviously elevated MVD and VEGF protein level, P<0.01. Compared with LS group, HS group presented obviously increased LVEF, LVFS, P<0.01 and decreased LVEDd, LVESd, CVF, P<0.05; elevated MVD and VEGF protein level, P<0.01 or P<0.05. Conclusion: Liraglutide could improve angiogenesis in AMI rats which might be related to increased VEGF expression and reduced collagen deposition; therefore improve left ventricular systolic function for cardiac protection. The effect had certain relationship to liraglutide dosage.
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XYL1 gene,which encodes xylose reductase with dual coenzyme activity from Candida tropicalis,was transformed into Pichia pastoris X-33 by expression vector pGAPZB.The recombination strain was immobilized in Ca-alginate beads and fermentation characterization is studied using corn cob hydrolysates.Fermentation conditions were as follow:initial pH value 6.0,30℃,initial cell concentration of 20%,the Liquid volume of 28%,rotation speed 130r/min.The average xylitol yield was 37.5% on the optimum condition.This result is expected to provide a new alternative method for producing xylitol on a large scale by bioconversion.