Evaluation of the value of anaerobic culture in bacterial contamination forapheresis platelets / 中国输血杂志

Objective To determine the potential deficiency due to lack of anaerobic culture and evaluate the effect to reduce adverse reaction associated to transfusion-translated bacterial infection.Methods The result of 9 758 units of apheresis platelet concentrates (PCs)detected with automated microbial detection system were reviewed and the medical records of the patients that received the contaminated PCs were followed.Results The confirmed positive rates by aerobic and anaerobic cultures were 0.06％ (6/9 758)and 0.16％ (16/9 758),respectively.In 10 of 16 yield cases,only the anaerobic culture was positive.The most of the bacterial detected by anaerobic culture only were Propionibacterium acnes.Their mean detection time from inoculation was 96.8±18.21 hours.Conclusion Addition of anaerobic culture would enhance the detection of bacterial contamination in PCs.However,since only slow-growing bacteria were detected,and because their clinical significance was debatable,blood service should select feasible and costeffective projects using only aerobic bottle for bacterial screening,like the majority of licensed blood centers in North America and Hong Kong,China.

Isolation, culture and identification of bacterial strains from tree shrews feces / 中国比较医学杂志

Objective Study the fecal flora diversity of the tree shrew , to provide a basis data of fecal bacteria of feeding the tree shrew .Methods Ten tree shrews were used in this study .The Stools of the animals were respectively cultured with oxygen and without oxygen to isolate the bacterial .Then the PCR-amplified 16S rRNA of the bacterial was sequenced and analyzed .Results 25 bacterial strains belonging to ten bacterial species were isolated by anaerobic incubation , and 25 bacterial strains belonging to twelve bacterial species were isolated by aerobic incubation .Proteus vulgaris, Enterococcus faecalis, Escherichia fergusonii, Enterococcus faecium, Shigella flexneri, Shigella sonnei, Staphylococcus aureus , Aeromonas salmonicida subsp .masoucida , Rahnella aquatilis , Exiguobacterium aquaticum , Raoultella terrigena , and Escherichia coli were identified in this study .Conclusions There is a fecal flora diversity of the tree shrew, and the Proteus vulgaris , Escherichia fergusonii and Enterococcus faecium may be the major parasitic flora .

Establishment of multiplex PCR for the rapid identification and toxin detection of Clostridium difficile strains / 中华微生物学和免疫学杂志

Objective To design a multiplex PCR for simultaneous identification and toxigenic type characterization of Clostridium difficile isolates. MethodsThree pairs of primers were designed for the amplification of a species-specific internal fragment of the tpi( triose phosphate isomerase) gene, an internal fragment of the tcdB ( toxin B) gene, and an internal fragment of the tcdA ( toxin A) gene. Twenty-one standard strains including Clostridium difficile ATCC 9689 and 47 isolates of Clostridium difficile were applied for the assessment of detection limit, specificity and detections of the multiplex PCR, respectively. Toxin A and Toxin B of 47 isolates were analyzed by ELISA. ResultsThe detection limit for DNA concentration of the multiplex PCR was 0.5 pg/μl. The specificity was determined to be 100％. Among the results of 47 isolates detected by multiplex PC R, 37 strains were tpi ( + )/tcdA (+)/tcdB ( + ), 10 strains were tpi ( + )/tcdA (-)/tcdB ( - ). Tpi ( + )/tcdA ( - )/tcdB ( + ) was not found. The toxin detection of 47 isolates by ELISA showed that 20 isolates were positive and 27 isolates were negative. Twenty isolates of toxin (+) by ELISA were all tpi( +)/tcdA( +)/tcdB(+) by multiplex PCR. ConclusionThe multiplex PCR method combined diagnosis and toxigenic type characterization contributes to the diagnosis for Clostridium difficile infection.

Research on Clostridium Difficile Infection in Clinic Patients Feces Specimen / 中华医院感染学杂志

OBJECTIVE This study was to investigate the carrier and infection of Clostridium difficile in clinic feces specimen,to analyze clinic characteristics,and to improve isolation rate and to provide basis on efficient prevention.METHODS C.difficile toxin A&B kit and anaerobic culture was conducted in 20 cases with diarrhea.Colonies suspected to be C.difficile,on the basis of their macroscopic appearance and characteristic odor,oxygen tolerance experiment,were confirmed by their biochemical characteristics(API 20A,bioMerieux).RESULTS After C.difficile selective culture,8 suspected colonies from 20 feces specimen were conducted by feces smear and oxygen tolerance experiment.6 of 8 was G+ rod bacteria with positive oxygen tolerance experiment.4 stains of C.difficile were identified by API 20A,positive rate was 20%;toxin detect was positive in 1 specimen(5%).CONCLUSIONS Infection of C.difficile Is associated with the basic disease.Watery feces specimen was prone to culture positive.

Anaerobic Bacterial Isolation in Patients with Chronic Prostatitis Syndrome / 대한비뇨기과학회지

In a general way, anaerobic isolation is troublesome and needs competent personnel and fittings. In addition, bacterial isolation from the prostate is disappointing because of difficulty in interpretation of the results. In this experiment, we tried the best way to isolate anaerobes from the prostate in terms of processing of the specimens such as catching, transportation, etc. We performed this antegrade experiment for 12 months in 1992 and got the results from 43 patients with chronic prostatitis syndrome as follows. l. Age distribution was in broad range between 20 and 54 showing peak incidence in 31-40 years(49% ) and the next in 20-30 years( 30%). 2. Subjective symptoms and signs consisted of perineal discomfort, suprapubic discomfort, frequency, urethral discomfort, dysuria, morning drop, testicular discomfort, and hemospermia 3. Majority of the cases( 36 cases. 83.7% ) were normal in the microscopy of VB1 EPS of 31 cases(72%) showed WBC more than 10/HPF. VB3 of 23 cases(53.5% ) showed than l0/HPF 4. A total of 40 cases showed aerobes in EPS and/or VB3 by culture. However, only 8 cases showed aerobes in EPS and/or VB, exclusive of 32 cases in which aerobes also appeared in VB1. 5. Anaerobic bacteria were cultured only from EPS for a total of 8 cases. There were 2 cases with Bacteroides species, 2 cases with Prevotella bivia, 2 cases with Peptostreptococcus anaerobrus,2 cases with Actinomyces meyeri, 1 case with Eubacterium lentum and 1 case with Eubacterium limosum.

The Quality Control of Immunofluorescent Antibody Test and Anaerobic Culture in Study of Bacteroides and Fusobacterium / 第三军医大学学报

A comparative study between the indirect immunofluorescent antibody test and anaerobic culture in the study of Bacteroides fragilis, Bacteroides melani-nogenicus, and Fusobacterium nucleatum in 374 clinical specimens was carried out. The data concerning the quality control of the two tests including the rate of correlation, the difference of the sensitivity and specificity,the predictive values, and the time of antiserum preservation were analyzed. It was found that immunofluorescent antibody test had a higher rate of detection than anaerobic culture. It can be cartain that if immunofluorescent antibody test is negtive, it is more likely that the anaerobic bacteria are abscent. The rate of correlation between the two tests was 88.5~95.99%. In addition, immunofluorescent antibody test can be used to verify the quality of anaerobic culture. It was found that our routine culture for B, fragilis was inperfect and waited for improvement.