RESUMO
The aim of the current study is to evaluate the effect of ethanolic seed extract of Caesalpinia bonduella (ESECB)on altered hormonal levels in mifebristone-induced polycystic ovarian syndrome (PCOS) female rats. PCOS wasinduced in female rats by a daily administration of mifepristone (4 mg/kg b.w.) for 8 days which was confirmed bythe persistent estrous cycle. Once PCOS was induced, the rats were treated with ESECB 200 and 400 mg/kg b.w.p.o. for 28 days, and the hormonal parameters were analyzed. Metformin was used as a standard drug. A significantincrease (p < 0.01) in the luteinizing hormone, Prolactin, insulin, testosterone, estrone, and estradiol levels anddecrease in follicle stimulating hormone and progesterone hormone levels in the PCOS rats were observed, whichwere normalized in the ESECB drug-treated animals. Among the two concentrations, 400 mg/kg b.w of ESECB drugtreatment showed significant effect in correcting this hormonal imbalance. Its effect was comparable to the positivecontrol used in the present study. It can be concluded that the ESECB can be used as a potential ovulation inducerin the treatment of PCOS
RESUMO
The antimicrobial activity of Caesalpinia bonducella extracts such as CLC, CLE, CSC, and CSE and thephytoconstituents such as β-Sitosterol (LC3) isolated from CLC and methyl (4E)-5-{2-[(1E)-buta-1,3-dien-1-yl]-4,6-dihydroxyphenyl} pent-4-enoate (SC2) isolated from CSC were evaluated on gram-positive and gram-negativebacteria. The extracts and isolated compounds were found to have moderate-to-significant bacterial inhibition. Thesignificant activity was observed in the inhibition of Pseudomonas aeruginosa by CLC extract (16.10 ± 1.10 mm),whereas the isolated phytocomponent SC2 showed the highest inhibition (16.50 ± 0.58 mm). Further, the isolatedcompounds were subjected to molecular docking studies of the bacterial DNA Gyrase. The in silico study showedthe docking energy of −6.4 and three hydrogen bonding. This in vitro and in silico analysis of extracts and isolatedphytocomponents of C. bonducella helps to understand and evaluate the therapeutic efficacy to cure infectiousdiseases and also supports the traditional medicinal claim as an antibiotic.
RESUMO
Background: An increase in the incidence and prevalence of hyperlipidemia in India has led to an increased demand for drugs with a lipid lowering potential. Thus, the present study was undertaken to evaluate the hypolipidemic activity of ethanolic extract of seed kernel of Caesalpinia bonducella Fleming (EESKCB) in albino rats fed with high fat diet.Methods: The extract was daily administered to healthy adult albino rats of either sex, fed with high fat diet for a period of 60 days. At the end of 60 days, i.e. on the 61st day, serum cholesterol, serum triglyceride, serum low density lipoprotein cholesterol, serum high density lipoprotein cholesterol, atherogenic index, the body weights of the rats and the serum levels of malondialdehyde (MDA) and catalase were estimated. Phytochemical analysis of the plant extract was also carried out. For statistical analysis, the methods employed were One-way ANOVA, Dunnett’s multiple comparison test and Student’s ‘t’ test (Paired). Values of p < 0.05 were considered significant.Results: The test drug brought about significant (p<0.05) decrease in the raised levels of all the lipid parameters except the High density lipoprotein cholesterol. There was also a significant (p<0.05) increase in the serum levels of catalase and a significant (p<0.05) reduction in the serum levels of MDA in the test drug treated group. The test drug also significantly (p<0.05) inhibited the rise in body weights of the rats.Conclusions: From this study, it can be said that the seed kernel of Caesalpinia bonducella Fleming has significant hypolipidemic activity.