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Objective To investigate the protective effect of Mongolian Medicine Youning Bawei Powder(MMYBP)on the liver of non-alcoholic fatty liver disease(NAFLD)model rats and its mechanism.Methods SD rats were randomly divided into normal group,model group,Yi Shan Fu group,MMYBP high and low dose groups.The normal group was fed with ordinary diet,and the other four groups were injected with dexamethasone and fed with high-fat diet,then establish NAFLD model.NAFLD model was treated with Yi Shan Fu,MMYBP high dose and low dose for one month.The liver index and pathological changes of liver tissue were detected.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG)and liver triglyceride(TG),malondialdehyde(MDA),superoxide dismutase(SOD)and free fatty acid(FFA)indexes were measured by enzyme linked immunosorbent assay(ELISA).The expression level of CYP2E1 mRNA in liver tissue was measured by reverse transcription PCR(RT-PCR).The expression of cytochrome P4502E1(CYP2E1)protein in liver tissue was measured by Western blot.Results The liver index of MMYBP high dose group was significantly lower than that of model group(P<0.05).The contents of AST,ALT and TG in serum were significantly lower than those in model group(P<0.05).The contents of TG,FFA and MDA in liver tissue were significantly lower than those in model group(P<0.05).The pathological changes of liver were significantly improved compared with the model group.The expression levels of CYP2E1 protein and CYP2E1 mRNA in liver tissue were significantly lower than those in model group.Conclusion MMYBP can reduce the expression level of CYP2E1 protein and CYP2E1 mRNA related to lipid metabolism in NAFLD,improve liver function and lipid metabolism disorder,reduce lipid and protect liver in NAFLD,and delay the progression of NAFLD to liver fibrosis and cirrhosis.
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OBJECTIVE: To observe the effect of electroacupuncture(EA) of "Fenglong" (ST 40) and "Sanyinjiao" (SP 6) on lipid metabolic disorder, insulin resistance (IR) and expression of sterol regulatory element blinding protein-1 (SREBP-1) c and fatty acid synthase (FAS) proteins in the liver tissue in hyperlipidemia rats with IR, so as to reveal its mechanisms underlying improvement of IR. METHODS: Forty male SD rats were randomly divided into blank control, model, medication and EA groups (n＝8 in each group). The IR model was established by feeding the rat with high-fat diet. Rats of the medication group were treated by intragastric administration of pioglitazone (10 mL/kg). For rats of the EA group, EA (2 Hz/100 Hz，1 mA) was applied to bilateral ST 40 and SP 6, once daily for 14 days. The insulin sensitivity index (ISI) was assessed by calculating 60－120 min glucose infusion rate (GIR 60－120) with euglycemic hyperinsulinemic clamp in reference to Kraegen's and colleagues' methods. Fasting blood samples (10 mL) were collected and analyzed for fasting blood glucose (FBG) using enzyme method, serum fasting insulin(FINS) using ELISA, free fatty acid(FFA) using spectrophotometry, and total triglyceride(TG) and total cholesterol(TC) employing glycerine phosphate oxidase peroxidase (GPO-PAP) assay, low density lipoprotein(LDL), high density lipoprotein(HDL) levels using combined filiter paper activity and lipase activity methods, respectively. The IR level was assessed by calculating homeostatic model assessment of insulin resistance (HOMA-IR) using the formula (FBG×FINS)/22.5. The expression levels of SREBP-1 c and FAS proteins in the liver tissue were detected by Western blot. RESULTS: Following modeling, the GIR 60－120 and serum HDL were significantly decreased(P0.05). CONCLUSION: EA intervention is able to improve the disorder of lipid metabolism of IR rats, which may be associated with its effects in reducing the expression of SREBP-1 c and FAS proteins and in lowering the synthesis of fatty acid.
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Objective To study the expression of inflammatory factors in the saliva and the stability of implants after implant denture repair in pa-tients with dysplasia of the dentition defect ,and to analyze the prognosis of patients with lipid metabolic disorder. Methods The dentition defect patients with lipid metabolism disorder were selected as the experimental group. Patients with lipid defect were selected as the control group. Saliva was collected before implantation,1 month and 3 months after implantation. ELISA method was used to detect IL-6,IL-1βand TNF-α. Osstell ISQTM radio frequency analyzer was used to detect the stability of the implants and the periodontal status was detected by Florida probe. Re-sults There were no significant differences in the depth of implant probing(PD),bleeding index(BI)and ISQ between the two groups by radio frequency analyzer(all P>0.05). The levels of IL-6,IL-1βand TNF-αin the pre-implantation,1 month and 3 months after implantation were sig-nificantly higher than those in the control group(all P0.05). Conclusion The patients with dyslipidemia after implantation have good implant stability as those with normal blood lipid ,and the patients with dyslipidemia can also obtain good prognosis.
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Diseases of glucose and lipid metabolism disorder,presented rather complicated pathological mechanism,often with clinical pattern of multiple concurrent diseases.Therefore,the traditional single-disease based on treatment methods need improving.In view of plenty of clinical practice,theatrical and fundamental researches,the pathological mechanisms of some chronic disorders,such as hyperlipidemia,nonalcoholic fatty liver disease,type 2 diabetes,hypertension,atherosclerosis and severe cardiovascular complications,resulted from the impairment in the metabolism of glucose and lipid were investigated using the method of integrated Chinese and western medicine.Overall,the features of these diseases and their common characteristics were discovered,and accordingly we defined the new concept of glucolipid metabolic disease (GLMD) and put forward the concept of pivot liver of metabolic regulation system.In addition,we developed the therapeutic strategy of modulating liver,starting pivot and cleaning turbidity,for the comprehensive and integrated treatment and prevention of these diseases.The theory of GLMD shared the critical characteristics with precision medicine,taking its own specialty.Finally,the content and approaches for the research of GLMD were proposed,and some essential and core fields in the precision medical research for GLMD were profoundly analyzed and prospected.
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Hyperuricemia(HUA),which can induce oxidative stress,endothelial dysfunction,and inflammation,is closely relevant to the metabolic syndrome(MS),such as obesity,impaired fasting blood glucose,hyper-triglyceridemia,and hypertension. Moreover,HUA is of reciprocal causation with insulin resistance(IR). Here,for the prevention and treatment of metabolic diseases such as HUA and MS,the interaction and the regulatory mechanism between MS and HUA are summarized,and the situation that HUA is an important complication of MS is illustrated.
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To investigate the significance of liver biopsy in children with nephrotic syndrome,liver and renal biopsy samples were simultaneously examined with light microscopy,elctronic microscopy,and immunofluorescence in 10 patients. The results showed that all patients had abnormal liver histologic changes.Focal fatty degeneration in liver tissue was found under microscopic examination,and cytolysis and mild fatty degeneration were found in hepatocytes under electronic microscopic examination.The results suggested that disorder of lipid metabolism not only was toxic to glomeruli, but also deleterious to hepatocytes,then in turn enhanced abnormality in lipid metabolism. It is believed that hyperlipidemia is related to an increased anabolism of lipid and apolipoprotein, and reduction in catabolism of chylomicron and very low density lipoprotein .Therefore, with treatment of hyperlipidemia in these patients, it is possible to arrest progressive renal injury.
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Objective To investigate the therapeutic effect of Jiangzhi Decoction(JD) for the treatment of disordered lipid metabolism.Methods With Zhibituo Capsule as the control capsule,a random,paralleled and positive controlled clinical trial was carried out.A total of 64 patients were equally randomized into the treatment group and control group,and the treatment lasted 4 weeks.Results After administration,the level of total cholesterol(TC),triglyceride(TG),and arteriosclerosis index(AI) were reduced,the high density lipoprotein cholesterol(HDL-C) level was increased,and the sum of the decrease of electrocardiogram(ECG) ST segment was markedly improved in the treatment group,the difference being significant compared with those before treatment.In the treatment group,oppressive pain in the chest and hypochondrium,dizziness,limb numbness,poor appetite and headache were much relieved,and the relief of oppressive pain in the chest and hypochondrium,dizziness,and poor appetite was obvious as compared with the control group.Conclusion JD has good curative effect on disordered lipid metabolism with stagnation of phlegm and blood-stasis,in particular on relieving the clinical symptoms.
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AIM:To investigate the effect of Haikun Shenxi Capsule(fucoidan)on disorder of lipid metabolism on the patients with chronic kidney disease(CKD).METHODS:98 patients with CKD 3、4 stage were divided into 2 groups at random:control group were given routine therapy and therapy group were given routine therapy plus Haikun Shenxi Capsule.The changes of the levels of TCH,TG,HDL-Ch,LDL-Ch,VLDL-Ch,ApoA-1,ApoB in 2 groups were observed.RESULTS:The level of TCH,TG decreased significantly(P 0.05).CONCLUSION:Haikun Shenxi Capsule has a positive effect on disorder of lipid metabolism on the patients with CKD.