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Objective: Securidaca inappendiculata is a medicinal plant frequently used in the treatment of inflammatory diseases in south China. In this study, we aimed to explore its bioactive constituent which contributes to the anti-inflammatory activity. Methods: Polyphenol-enriched and polyphenol-deprived fractions (PRF and PDF, respectively) were separated from the ethanolic extract by HPD300 macroporous resin-based method, and their anti-inflammatory activities were investigated on a lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats. The possible mechanism of action in alleviating acute inflammation was studied using RAW264.7 cells. Results: Both Folin-Ciocalteu and
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Objective To study the chemical constituents from the rhizomes of Securidaca inappendiculata. Methods The concrete exacted by 95% EtOH from the rhizomes of S. inappendiculata was isolated and purified by chromatography on macroporous resin, silica gel, MPLC, gel, preparative HPLC, etc. The structures of the chemical constituents were elucidated by means of physicochemical properties and spectroscopic analysis. Results Five compounds were isolated and identified as 2- methylene-butanoic acid 4-O-[(β-D-glucopyranosyl)oxy]-intramol-1,6’-ester (1), 3-methoxyl-4-O-β-D-glucopyranosyloxy-benzoic acid methyl ester (2), threo-4,7,9,9’-tetrahydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside (3), eucomegastigside A (4), and acernikol-4″-O-β-D-glucopyranoside (5). Conclusion Compound 1 is a new hemiterpene glycoside named securiterpenoside D, and compounds 2-4 are isolated from Polygalaceae for the first time.
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Nine compounds, including five lignan glycosides (1-5), three sucrose esters (6-8), and one organic acid ester (9), were isolated from the ethanol extract of the roots of Securidaca inappendiculata by various chromatographic methods including silica gel, MPLC and preparative HPLC. Their structures were elucidated as acernikol-4″-O-β-D-glucopyranoside (1), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside (2), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside (3), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9'-O-β-D-glucopyranoside (4), (7R, 8S)-5-methoxydihydrodehy-drodiconiferyl alcohol 4-O-β-D-glucopyranoside (5), 3, 6'-O-diferuloylsucrose (6), 3-O-feruloyl-6'-O-sinapoylsucrose (7), sibricose A5 (8), and mehyl ferulate (9) on the basis of 1H-, 13C-NMR and MS experiments. Compounds 1-5, 8, and 9 were isolated from the Securidaca genus for the first time. Compounds 2, 3, and 7 exhibited weak cytotoxic activities against Hela and MCF-7 cell lines.
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AIM To study the chemical constituents from Securidaca inappendiculata Hassk..METHODS The dichloromethane and ethyl acetate fractions of S.inappendiculata 95％ ethanol extract were isolated and purified by silica,gel column and recrystallization,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and identified as 8-hydroxy-1,3,4-trimethoxyxanthone (1),1,2,7-trimethoxyxanth-one (2),4-hydroxy-3,5-dimethoxybenzaldehyde (3),sesamin (4),2-methoxy-3,4-methylenedioxybenzophenone (5),1,3,7-trihydroxy-4-methoxyxanthone (6),1,3,7-t-rihydroxy-2-methoxyxanthone (7),2-hydroxy-1,7-dimethoxyxanthone (8),3,8-dih-ydroxy-1,4-dimethoxyxanthone (9),oα-spinasterol (10).CONCLUSION Compound 1 is first found in the form of natural product,compounds 2-4 are isolated from genus Securidaca for the first time.
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Object To investigate the chemical constituents of Securidaca inappendiculata Hassk Methods Compounds of 95% alcohol extract from the stem of S inappendiculata were isolated by column chromatography and Medium Pressure Liquid Chromatography, respectively The structures of the compounds were elucidated by chemical and spectral (UV, IR, MS, 1HNMR adn 13 CNMR) analyses Results Six compounds were isolated and identified as: 4, 4′ dimethyl 1, 7 heptanedioic acid (Ⅰ), inositol (Ⅱ), stigmasterol (Ⅲ), vittadinoside (Ⅳ), rhamnose (Ⅴ), sucrose (Ⅵ) Conclusion For the first time, compound Ⅰ was obtained from the plant and other compounds were isolated from Securidaca Mill