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Aims: This study aims to evaluate the five clones of jojoba plant under the influence of irrigation intervals and the impact on growth and yield of chemical content. Study Design: Two field experiments were carried out for five clones (S-L, S-610, S- 700, S-B and S-G), to study the effect of irrigation intervals; one week (control), two weeks and three weeks) on jojoba plants at aged three and thirteen years from planting to investigation growth, yield characters and content seed from oil and some chemical contents. Place and Duration of Study: Almaghara Research and Production Station (latitude: 30,717993''N, longitude: 33, 329103 E) which follow Desert Research Center, Agriculture Ministry, Egypt, at 2015l2016 and 2016/2017 respectively. Methodology: The experimental rows (5 plants) were assigned for each clone in three replications. The farming distances were 4 x 2 meters. Mixed seedlings of both females and males were planted at a rate of one male seedling for every six female seedlings to allow cross-fertilization of each clone. All clones were treatment at three times (October, March and April). The plants were collected on 22nd April to determine the growth, yield characters and some chemical content. Statistical analyses and mean comparisons were conducted using MSTATC software. Results: The jojoba plant was affected by increased irrigation interval, and it was clear in all clones. Irrigation every three weeks had an impact of irrigation every week. The effect of irrigation interval varied from one clone to another and the lowest was s-700. The older seedlings are less affected by irrigation interval than small seedlings. The best results were category s-700 at the age of thirteen years and irrigation every two weeks. Conclusion: All variable of studied confirmed that clones of jojoba had a significant effect with irrigation interval at one week but decrease value at three weeks. Jojoba plants tolerance to water stress but irrigation interval every week increased seed yield.
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OBJETIVO: Determinar la prevalencia de los genotipos del papiloma virus humano en muestras cérvico-uterinas y su relación con los factores de riesgo en mujeres con vida sexual activa de la ciudad de Cuenca. METODOLOGÍA: Estudio observacional de tipo transversal con una muestra de 500 mujeres del cantón Cuenca de los Hospitales Monte Sinai y del Instituto Ecuatoriano de Seguridad Social (IESS). Los exámenes se realizaron en el laboratorio de Biología Molecular BIONCOGEN de la ciudad de Cuenca y se utilizó la técnica de PCR en Tiempo Real más el examen de Papanicolaou. RESULTADOS: La prevalencia del VPH fue de 78.4% y el genotipo más frecuente es el HPV 16 con el 26,2%. El NIC I con el 27% constituye la patología ginecológica más frecuente. CONCLUSIÓN: Los genotipos 16 /18 representan los tipos de VPH que están relacionados directa-mente con el desarrollo de cáncer cérvico uterino ya que al correlacionar con la citología (Papani-colau) se demuestra que la célula sufre cambios morfológicos (coilocitosis).
OBJECTIVE: To determine the prevalence of hu-man papilloma virus genotypes in cervical-uterine samples and their relation to risk factors in women with active sex life in Cuenca. METHODOLOGY: It is a cross-sectional observatio-nal study with a sample of 500 women from Cuen-ca who were attended in the Monte Sinai Hospi-tal and the Ecuadorian Institute of Social Security (IESS). The exams were carried out in the BIONCO-GEN Molecular Biology Laboratory which is loca-ted in Cuenca. The Real Time PCR technique plus the Pap examination were used. RESULTS: The prevalence of HPV was 78.4 % and the most frequent genotype was the HPV 16 with 26.2 %. On the other hand, the CIN I with 27% is the most frequent gynecological pathology. CONCLUSION: The genotypes 16/18 represent the types of HPV that are directly related to the de-velopment of cervical cancer because when it is correlated with cytology (Pap) it is demonstrated that the cell undergoes morphological changes (koilocytosis).Keywords: Genotype, Human papillomavirus 16, Human papillomavirus 18, Clinical Laboratory Te-chniques, PCR; Risk factors; Monte Sinai Hospital, Hospital of the Ecuadorian Institute of Social Secu-rity, Cuenca-Ecuador.
Assuntos
Humanos , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Papillomavirus Humano 16 , Papillomavirus Humano 18 , Genótipo , Reação em Cadeia da Polimerase , Fatores de Risco , Teste de PapanicolaouRESUMO
Cutaneous leishmaniasis (CL) is a neglected clinical form of public health importance that is quite prevalent in the northern and eastern parts of Egypt. A comprehensive study over seven years (January 2005-December 2011) was conducted to track CL transmission with respect to both sandfly vectors and animal reservoirs. The study identified six sandfly species collected from different districts in North Sinai: Phlebotomus papatasi, Phlebotomus kazeruni, Phlebotomus sergenti, Phlebotomus alexandri, Sergentomyia antennata and Sergentomyia clydei. Leishmania (-)-like flagellates were identified in 15 P. papatasi individuals (0.5% of 3,008 dissected females). Rodent populations were sampled in the same districts where sandflies were collected and eight species were identified: Rattus norvegicus (n = 39), Rattus rattus frugivorous (n = 13), Rattus rattus alexandrinus (n = 4), Gerbillus pyramidum floweri (n = 38), Gerbillus andersoni (n = 28), Mus musculus (n = 5), Meriones sacramenti (n = 22) and Meriones crassus (n = 10). Thirty-two rodents were found to be positive for Leishmania infection (20.12% of 159 examined rodents). Only Leishmania major was isolated and identified in 100% of the parasite samples. The diversity of both the vector and rodent populations was examined using diversity indices and clustering approaches.
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Animais , Feminino , Masculino , Reservatórios de Doenças/parasitologia , Ecossistema , Insetos Vetores/parasitologia , Leishmaniose Cutânea/transmissão , Psychodidae/parasitologia , Roedores/parasitologia , Reservatórios de Doenças/classificação , Egito , Insetos Vetores/classificação , Psychodidae/classificação , Roedores/classificaçãoRESUMO
Introduction: In past decades, leishmaniasis burden has been low across Egypt; however, changing environment and land use has placed several parts of the country at risk. As a consequence, leishmaniasis has become a particularly difficult health problem, both for local inhabitants and for multinational military personnel. Methods: To evaluate coarse-resolution aspects of the ecology of leishmaniasis transmission, collection records for sandflies and Leishmania species were obtained from diverse sources. To characterize environmental variation across the country, we used multitemporal Land Surface Temperature (LST) and Normalized Difference Vegetation Index (NDVI) data from the Moderate Resolution Imaging Spectroradiometer (MODIS) for 2005-2011. Ecological niche models were generated using MaxEnt, and results were analyzed using background similarity tests to assess whether associations among vectors and parasites (i.e., niche similarity) can be detected across broad geographic regions. Results: We found niche similarity only between one vector species and its corresponding parasite species (i.e., Phlebotomus papatasi with Leishmania major), suggesting that geographic ranges of zoonotic cutaneous leishmaniasis and its potential vector may overlap, but under distinct environmental associations. Other associations (e.g., P. sergenti with L. major) were not supported. Mapping suitable areas for each species suggested that northeastern Egypt is particularly at risk because both parasites have potential to circulate. Conclusions: Ecological niche modeling approaches can be used as a first-pass assessment of vector-parasite interactions, offering useful insights into constraints on the geography of transmission patterns of leishmaniasis. .