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Abstract Objective: Analyze sex hormone's influence during Chagas disease. Methods: Male and female BALB/c mice were divided into six groups, four experimental (sham, orchiectomized, orchiectomized and supplemented with estradiol, orchiectomized supplemented with testosterone, oophorectomized, oophorectomized and supplemented with estradiol, and oophorectomized and supplemented with testosterone), and two control (healthy and intraperitoneally with T. cruzi strain NINOA infected). Clinical data were recorded daily, parasitemia was evaluated using a Neubauer chamber during the infection, and heart histopathological analysis was performed using the paraffin embedding technique. To analyze parasitemia curves and the area under the parametric curves, two-way ANOVA test was performed to correlate groups' data. P-values < 0.05 were considered statistically significant. Results: Higher mortality rates, cardiomegaly, hepatomegaly, ascites, edema, higher parasitemia levels, more amastigote nests, and more severe inflammatory infiltrate were found in higher testosterone concentration mice, whereas in higher estradiol concentration groups, paresia, prostration, edema, and necrosis were found. Conclusions: Our results showed that testosterone increased infection severity, whereas estradiol had the opposite effect. This research improves the understanding of sex hormones´ infuence upon this infection to contribute with the handling of Chagas´ disease.
Resumen Objetivo: Analizar la influencia de las hormonas durante la enfermedad de Chagas. Métodos: Se separaron grupos de ratones macho y hembras BALB/c, todos infectados con T. cruzi (cepa NINOA), 4 grupos experimentales de machos (Sham, orquidectamizados, orquidectimezados y suplementados con estradiol, orquidectamizaos y suplementados con testosterona). 4 grupos experimentales de hembras (oforectomizadas, oforectomizadas y suplementadas con estradiol, oforectomizadas y suplementadas con testosterona y sham), and y dos grupos control para cada sexo (sin infección e infectados intraperitonealmente con T. cruzi (cepa NINOA). Los datos clínicos fueron registrados diariamente, la parasitemia fue evaluada durante toda la infección utilizando una cámara de Neubauer y el análisis histopatológico del corazón fue realizada con la técnica de inclusión en parafina. Para el análisis de las curvas de parasitemia y el área bajo la curva, se realizó una prueba de ANOVA de dos vías, p < 0.05 fueron considerados estadísticamente diferentes. Resultados: Las mayores tasas de mortalidad, cardiomegalia, hepatomegalia y mayor infiltrado inflamatorio, se encontró en los ratones con una mayor concentración de testosterona. En contraste los ratones con mayor concentración de estradiol presentaron paresia, postración edema y necrosis. Conclusiones: Nuestros resultados ponen en manifiesto que la testosterona incrementa la severidad del curso de la enfermedad de Chagas, mientras que el estradiol tuvo el efecto opuesto. Este trabajo mejora el entendimiento del rol que juegan las hormonas sexuales en esta infección para contribuir en un mejor manejo de la enfermedad de Chagas.
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ABSTRACT Purpose: Statins are one of the most prescribed classes of drugs worldwide to treat hypercholesterolemia and dyslipidemia. By lowering the level of cholesterol, the use of statin could cause a reduction in testosterone levels. The objective was to evaluate whether the continued use of statins in patients with hypercholesterolemia causes a deficiency in testosterone and other sex hormones. Materials and Methods: Systematic Review with Meta-analysis, performed in Embase, Medline and Cochrane databases, until May 2023; PROSPERO CRD42021270424protocol. Selection performed by two independent authors with subsequent conference in stages. Methodology based on PRISMA statement. There were selected comparative studies, prospective cohorts (CP), randomized clinical trials (RCT) and cross-sectional studies (CSS) with comparison of testosterone levels before and after statin administration and between groups. Bias analysis were evaluated with Cochrane Tool, The Newcastle-Ottawa Scale (NOS), and using the Assess the Quality of Cross-sectional studies (AXIS) tool. Results: There were found on MedLine, Embase and Cochrane, after selected comparative studies, 10CP and 6RCT and 6CSS for the meta-analysis. In the Forrest plot with 6CSS, a correlation between patients with continuous use of statins and a reduction in total testosterone was evidenced with a statistically significant reduction of 55.02ng/dL (95%CI=[39.40,70.64],I²=91%,p<0.00001). In the analysis with 5RCT, a reduction in the mean total testosterone in patients who started continuous statin use was evidenced, with a statistical significance of 13.12ng/dL (95%CI=[1.16,25.08],I²=0%,p=0.03). Furthermore, the analysis of all prospective studies with 15 articles showed a statistically significant reduction in the mean total testosterone of 9.11 ng/dL (95%CI=[0.16,18.06],I²=37%,p=0.04). A reduction in total testosterone has been shown in most studies and in its accumulated analysis after statin use. However, this decrease was not enough to reach levels below normal. Conclusion: Statins use causes a decrease in total testosterone, not enough to cause a drop below the normal range and also determines increase in FSH levels. No differences were found in LH, Estradiol, SHBG and Free Testosterone analysis.
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Se presenta un caso clínico de Síndrome de Klinefelter y se revisan que los aspectos en relación al sueño en estos pacientes, siendo relevante a ser abordado y estudiado debido a la relación causal entre el metabolismo de esteroides sexuales afectados. En especial la testosterona y cómo esto influye en la microarquitectura del sueño y la probabilidad de presentar síndrome de apnea obstructiva del sueño, con las repercusiones cognitivas que pueden sumarse a las ya descritas por el síndrome en si. De allí la importancia de un seguimiento y abordaje dirigido en este aspecto, al momento del diagnóstico y en el seguimiento a largo plazo.
A clinical case of Klinefelter's Syndrome is presented and the aspects related to sleep in these patients are reviewed, being relevant to be addressed and studied due to the causal relationship between the metabolism of affected sex steroids, especially testosterone and how this influences the microarchitecture of sleep and the probability of presenting obstructive sleep apnea syndrome with the cognitive repercussions that can be added to those already described by the syndrome itself. Hence the importance of a targeted follow-up and approach in this aspect, at the time of diagnosis and in long-term follow-up.
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Humanos , Masculino , Criança , Sono , Síndrome de Klinefelter/diagnóstico , Testosterona , Vitamina DRESUMO
Resumo Fundamento: A deficiência de testosterona (DT) é uma condição prevalente em nosso meio e ainda muito negligenciada. A hipertensão arterial (HA) é um de seus possíveis fatores associados. Objetivos: Determinar a prevalência de DT em uma população masculina hipertensa e os fatores associados à sua ocorrência, como idade, tempo de diagnóstico de HA, número de classes de anti-hipertensivos, índice de massa corporal (IMC), diabetes, dislipidemia, doença renal crônica (DRC), sintomas positivos de DT (questionário ADAM positivo) e uso de espironolactona. Métodos: Estudo transversal com aplicação do questionário ADAM, e avaliação de dados bioquímicos, clínicos e antropométricos. Os pacientes foram estratificados em grupos de DT e testosterona normal. As variáveis categóricas foram comparadas pelo teste do qui-quadrado e as variáveis contínuas pelo teste de Mann-Witney; as variáveis com significância (p<0,05) foram submetidas à regressão linear multivariada. Resultados: A prevalência de DT foi de 26,8%. Houve associação entre DT e IMC (p=0,0007), mas não houve com idade (p=0,0520), tempo de diagnóstico de HA (p=0,1418), número de classes de anti-hipertensivos (p=0,0732), diabetes (p=0,1112); dislipidemia (p=0,3888); presença de DRC (p=0,3321); uso de espironolactona (p=0,3546) e questionário ADAM positivo (p=0,2483). Conclusões: A prevalência de DT foi alta e houve associação positiva com IMC. A testosterona total (TT) declinou 8,44 ng/dL com o aumento de 1 kg/m2 no IMC e caiu 3,79 ng/dL com o avanço em um ano na idade.
Abstract Background: Testosterone deficiency (TD) is a prevalent condition in our midst and still very neglected. Arterial hypertension (AH) is one of the possible associated factors. Objectives: To determine the prevalence of TD in a hypertensive male population and the factors associated with its occurrence, such as age, time since hypertension diagnosis, number of antihypertensive classes, body mass index (BMI), diabetes, dyslipidemia, chronic kidney disease (CKD), positive symptoms of TD (positive ADAM questionnaire) and use of spironolactone. Methods: Cross-sectional study with administration of the ADAM questionnaire, assessment of biochemical, clinical, and anthropometric data. Patients were stratified into DT and normal testosterone groups. Categorical variables were compared using the chi-squared test and continuous variables using the Mann-Witney test; variables with significance (p<0,05) were analyzed by multivariable linear regression. Results: The prevalence of TD was 26.36%. There was an association between TD and body mass index (BMI) (p=0.0007) but there was no association with age (p=0.0520), time of hypertension diagnosis (p=0.1418), number of classes of antihypertensive drugs (p=0.732), diabetes (p=0.1112); dyslipidemia (p=0.3888); CKD (p=0.3321); use of spironolactone (p=0.3546) or positive ADAM questionnaire (p=0.2483). Conclusions: TD was highly prevalent and positively associated with BMI. Total testosterone (TT) declined by 8.44ng/dL with a one unit increase in BMI and dropped by 3.79ng/dL with a one-year increase in age.
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Abstract Introduction Anxiety, mood- and stress-related behaviors are regulated by sex hormones in pregnant and non-pregnant women. Very scarce information exists about the role of sex steroids in pregnant women displaying high levels of anxiety. Objective To determine sex hormones serum levels in pregnant women exhibiting high levels of anxiety symptoms. Method The Hamilton Anxiety Rating Scale (HARS/ HAM-A) was used to assess the intensity of anxiety symptoms in third-trimester pregnant women. Two groups were included in the study, pregnant women exhibiting severe anxiety (ANX; HARS scores ≥ 25; n = 101) and healthy control subjects (CTRL; n = 40) displaying lower scores for anxiety (HARS scores ≤ 7). Estradiol (E2), progesterone (P4), and testosterone (T) serum levels were measured using a standard chemiluminescent immunoassay. Bivariate and partial correlations were performed to detect significant associations between groups, clinical measures, biochemical data, and HARS scores. Results The anxiety group (ANX) showed an increase in E2 and T serum levels (p < .001) compared to CTRL. Conversely, significantly lower P4 levels were found in the symptomatic group (p < .001) as compared to the CTRL hormone values. The P4:E2 index was significantly reduced in pregnant women with high levels of anxiety (p < .001). Negative correlations between anxiety (HARS) scores, P4 serum levels (p = .02), and P4:E2 ratio (p = .04) were found in the symptomatic group. Conversely, T serum levels displayed a positive association (p = .001) with high levels of anxiety symptoms in the same group, after adjusting our data by clinical confounders. Discussion and conclusion Serum levels of sex-steroid hormones are altered in pregnant women exhibiting severe anxiety.
Resumen Introducción La ansiedad, el estado de ánimo y el estrés están regulados por diversos esteroides sexuales. Existe poca información sobre el papel que juegan estos esteroides en mujeres embarazadas con niveles elevados de ansiedad. Objetivo Determinar los niveles séricos de hormonas sexuales en mujeres embarazadas con altos índices de síntomas de ansiedad con respecto a mujeres gestantes sanas. Método Determinación de la intensidad de síntomas ansiosos empleando la escala de Hamilton de Ansiedad (HAM-A) en 141 mujeres embarazadas en el tercer trimestre de gestación. Cuantificación de los niveles séricos de estradiol (E2), progesterona (P4) y testosterona (T) por inmunoensayo estándar. Aplicación de las correlaciones de Pearson para detectar asociaciones entre parámetros clínicos y valores hormonales entre los grupos de estudio. Resultados Las mujeres con ansiedad severa (ANX; n = 101; HAM-A ≥ 25) mostraron niveles séricos más altos de E2 y T (p < .001), así como niveles más bajos de P4 (p < .001) en relación con el grupo control (CTRL, n = 40, HAM-A < 7). Se detectó una disminución significativa en el índice P4:E2 en el grupo de ANX (p < .001) y se observaron correlaciones negativas y positivas entre los puntajes elevados de ansiedad con los niveles circulantes de P4 (p = .02), en la taza P4:E2 (p = .04) y en los niveles séricos de T (p = .001) respectivamente, al ajustar nuestros datos con variables confusoras. Discusión y conclusión Los niveles circulantes de los esteroides sexuales se encontraron alterados en mujeres con ansiedad severa.
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Diabetic nephropathy is one of the most common and harmful microvascular complications of diabetes. In recent years, it has become a leading cause of end-stage renal disease due to its high incidence rate, challenging control, and poor prognosis. Studies have revealed that patients with diabetic nephropathy often exhibit imbalanced levels of sex hormones such as testosterone, dehydroepiandrosterone, estradiol, sex hormone binding globulin, and gonadotropin. Conversely, the imbalance of sex hormones can also influence the progression of diabetic nephropathy. Therefore, clarifying the potential relationship and interaction between sex hormones and diabetic nephropathy is essential for effective prevention and treatment of diabetic nephropathy. This warrants further research and consideration.
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Objective:To investigate the causal association between testosterone and nonalcoholic fatty liver disease(NAFLD) in men and women using a two-sample Mendelian randomization(MR) approach.Methods:Genetic variation in testosterone(total testosterone, bioavailable testosterone) and sex hormone-binding globulin(SHBG) in females and males was used as an instrumental variable using the genome-wide association study(GWAS) pooled data, and the inverse variance weighting method was applied. Inverse variance weighted(IVW) was used as the main analytical method, along with six univariate MR methods based on other modeling assumptions to assess the causal relationship between testosterone(total testosterone, bioavailable testosterone) as well as SHBG and NAFLD in women and men. In addition, NAFLD data from Finnish Biobank(FinnGen) were applied to validate the results of the exploratory analysis. Further, sensitivity analyses were performed to assess the level of heterogeneity, genetic pleiotropy, and stability of the instrumental variables using Cochran′ s Q test, MR-Egger regression, and leave-one-out methods. Results:The results of exploratory analysis of IVW model showed that bioavailable testosterone and SHBG were causally associated with NAFLD in women, for each unit increase in bioavailable testosterone levels, the risk of developing non-alcoholic fatty liver disease(NAFLD) rose by 24%( OR=1.24, 95% CI 1.07-1.43, P=0.004); and with each unit decrease in women′s SHBG, the NAFLD risk increased by 31%( OR=0.69, 95% CI 0.57-0.83, P<0.001). However, testosterone(total testosterone, bioavailable testosterone) as well as SHBG in men and female total testosterone did not show a causal relationship with NAFLD. The results of the other six MR methods were generally consistent with the IVW method. The results of the external validation data provided further evidence of a causal relationship between female bioavailable testosterone and SHBG and NAFLD. Conclusion:Elevated levels of bioavailable testosterone along lower levels of SHBG may increase the risk of developing NAFLD in women.
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Objective To explore the effects of spermary on sexual behavior,gonadal development and sperm count production.Methods Fifty-six male zebrafishes of 10-month-old AB strain after fertilization were randomly divided into 7 tanks and set up as the normal group,model group,positive control group(clomiphene citrate group,Wuzi Yanzong Pills group),and Spermary low,medium and high concentration groups,8 fishes in each group.Except for the normal group,cyclophosphamide was given in water solution to establish the zebrafish oligospermia model,respectively.On 9 d of modeling,the positive control group was ad-ministered at a concentration of 0.1 μg/mL in the clomiphene citrate group and 6 μg/mL in the Wuzi Yanzong Pill group;the spermary low,medium and high concentration groups were administered at 200,300,900 μg/mL,respectively.On 16 d of modeling,the normal female zebrafishes were placed in the culture tank of each group in a 1∶1 ratio,and the frequency of tail-chasing was observed and recorded in male and female fishes.The body length,weight,testis weight and sperm count were measured and the statistical analysis was performed by comparing the differences among the groups before and after drug administration(one-way ANOVA).Results Com-pared with the normal group,the frequency of tail-chasing of zebrafishes,body length,body weight,testis weight and sperm count in the model group were significantly decreased(P<0.05).Compared with the model group,the frequency of tail-chasing of zebrafishes,body length,body weight,testis weight and sperm count in the spermary low,medium and high concentration groups all were significantly increased(P<0.05),in which the vitality change of sexual behavior in the spermary medium concentration group was most obvious,and the increase of sperms count was most obvious.Conclusion A certain dose of spermary could effectively promote the sexual behavior,gonadal development and sperm count production in male zebrafish with oligospermia.The efficacy of spermary in enhancing the male sexual function and treating oligozoospermia and infertility de-serves to be studied.
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Comamonas testosteroni newly emerging microorganism previously known as Pseudomonas testosteroni is common environmental bacterium that is not known to be a part of the human commensal organism. Since its identification as a human pathogen in 1987, numerous reports have drizzled in, implicating this organism for various infections. Comamonas testosteroni are rare isolates in microbiology laboratories and have been infrequently reported as an infectious agent in routine clinical practice. Comamonas testosteroni has been rarely observed as an infectious agent in clinical practice. Comamonas testosteroni is rarely recognized as a human pathogen. Most of the reported cases are bloodstream infections. We report this pathogen from the stool of an immunocompromised 48-year-old male. The aim of this case report is to alert clinicians and laboratory physicians for the potential diagnosis and clinical approach of gastrointestinal infections caused by this organism.
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Objectives: The purpose of this study was to see the effect of parenteral testosterone injection on penile length, glans diameter, and diameter at the base of the penis in hypospadias patients before surgery. Material and Methods: The study was conducted from September 2019 to March 2021. This study comprised a total of 20 hypospadias patients. At a dose of 2 mg/kg body weight, an injection testosterone propionate was administered deep intramuscularly in three doses with a 3-week gap before reconstructive surgery. Before surgery, the penile length, glans diameter, and penile diameter at the base of the penis were all measured. Results: The mean increase in penile length, glans diameter, and diameter at the base of penis following parenteral testosterone therapy was 1.07 ± 0.23 cm (P < 0.001), 1.01 ± 0.46 cm (P < 0.001), and 0.92 ± 0.12 cm (P < 0.001), respectively. All three measurements were statistically significant. Conclusion: Intramuscular testosterone increased penis size, glans diameter, and penile diameter without causing any notable side effects. Development of fine pubic hair, acne, and aggressiveness is minor adverse effects.
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Abstract Objective: To evaluate hypothalamic-pi- tuitary-gonadal (HPG) axis alterations at 1 and 12 months after kidney transplan- tation (KT) and their association with in- sulin resistance. Methods: A retrospective clinical study was conducted in a tertiary care center in kidney transplantation recipients (KTRs) aged 18- 50 years with primary kidney disease and stable renal graft function. LH, FSH, E2/T, and HOMA-IR were assessed at 1 and 12 months after KT. Results: Twenty-five KTRs were included; 53% were men, and the mean age was 30.6±7.7 years. BMI was 22.3 (20.4-24.6) kg/m2, and 36% had hypogonadism at 1 month vs 8% at 12 months (p=0.001). Re- mission of hypogonadism was observed in all men, while in women, hypogonadotropic hypogonadism persisted in two KTRs at 12 months. A positive correlation between go- nadotrophins and age at 1 and 12 months was evident. Fifty-six percent of patients had insulin resistance (IR) at 1 month and 36% at 12 months (p=0.256). HOMA-IR showed a negative correlation with E2 (r=- 0.60; p=0.050) and T (r=-0.709; p=0.049) at 1 month, with no correlation at 12 months. HOMA-IR at 12 months after KT correlated positively with BMI (r=0.52; p=0.011) and tacrolimus dose (r=0.53; p=0.016). Conclusion: Successful KT restores the HPG axis in the first year. Hypogonadism had a negative correlation with IR in the early pe- riod after KT, but it was not significant at 12 months.
Resumo Objetivo: Avaliar as alterações do eixo hipotálamo-hipófise-gonadal (HHG) em 1 e 12 meses após transplante renal (TR) e sua associação com a resistência à insulina. Métodos: Foi realizado um estudo clínico retrospectivo em um centro de cuidados terciários em receptores de transplante renal (RTR) com idade entre 18-50 anos com doença renal primária e função do enxerto renal estável. LH, FSH, E2/T e HOMA-IR foram avaliados em 1 e 12 meses após o TR. Resultados: foram incluídos 25 RTR; 53% eram homens e a média de idade foi de 30,6±7,7 anos. O IMC foi de 22,3 (20,4-24,6) kg/m2 e 36% apresentaram hipogonadismo em 1 mês vs 8% aos 12 meses (p=0,001). A remissão do hipogonadismo foi observada em todos os homens, enquanto nas mulheres, o hipogonadismo hipogonadotrófico persistiu em dois RTR aos 12 meses. Ficou evidente uma correlação positiva entre gonadotrofinas e idade em 1 e 12 meses. Cinquenta e seis por cento dos pacientes apresentaram resistência à insulina (RI) em 1 mês e 36% aos 12 meses (p=0,256). O HOMA-IR mostrou uma correlação negativa com E2 (r=-0,60; p=0,050) e T (r=-0,709; p=0,049) em 1 mês, sem correlação em 12 meses. O HOMA-IR aos 12 meses após TR correlacionou-se positivamente com o IMC (r=0,52; p=0,011) e a dose de tacrolimus (r=0,53; p=0,016). Conclusão: O TR bem-sucedido restaura o eixo HHG no primeiro ano. O hipogonadismo apresentou uma correlação negativa com a RI no período inicial após o TR, mas essa correlação não foi significativa aos 12 meses.
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This study was designed to evaluate the cytohistomorphological and biochemical effect of alcoholic beverages on the prostate gland of adult male Wistar rats. Sixty-five (65) rats weighing between 180-230g were used for this experiment. They were randomly divided into 13 groups of five (5) animals each. Group 1 was the normal control. Group 2-13 were the experiment groups. Group 2, 3 and 4 were treated with 1.23mg/kg, 2.45mg/kg and 3.68mg/kg bodyweight of brandy respectively. Group 5, 6 and 7 were treated with 17.32mg/kg, 34.64mg/ kg and 51.96mg/kg body weight of beer respectively. Group 8, 9 and 10 were treated with 12.25mg/kg, 24.96mg/kg and 36.74mg/kg bodyweight of soured wine respectively. Group 11, 12 and 13 were treated with 1.73mg/kg, 3.46mg/kg and 5.20mg/kg bodyweight of dry gin respectively. Administration was done daily for 28 days and orally using orogastric tube. On the 29th day, the animals were sacrificed using chloroform inhalation anaesthesia. The blood samples were aspirated via cardiac puncture and centrifuged for biochemical analysis, and testicular tissues were harvested, fixed in 10% buffered formalin, processed, and stained with haematoxylin and eosin. Body weight showed significant (p<0.05) increase in brandy administered groups compared to control. For testicular weight, there was an insignificant increase in all the treated groups except the group administered with 3.69mg/kg which showed insignificant decrease compared to control. Results for TT showed a general significant (p<0.001) decrease in all administered groups compared to control. FSH showed significant (p<0.05;0.01;0.001) decrease in group administered 3.69mg/kg of brandy, all administered groups of soured wine and group administered 5.20mg/kg of dry gin compared to control. Histology showed narrowed and elongated lumen of seminiferous tubule, hypertrophied sertoli cells, destroyed interstitial cells of leydig, distorted seminiferous tubules with degenerating spermatogenic cells in the administered groups compared to control. In conclusion, alcohol beverages pose adverse effects on the testes.
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Loboob as a traditional drug in Iranis known for its beneficial effects on busulfan-induced oligospermia. In this experimental study, protective effects of loboob (a Persian traditional remedy) on sexual hormones, antioxidant levels and stereological changes of testis tissue were evaluated in an oligospermia rat model induced by busulfan. Fifty male rats were randomly divided into five different groups: control, received no treatments; and the other groups administrated with a single dose of busulfan (10 mg/kg body weight). After 30 days, these groups were treated with 0, 35, 70 or 140 mg/kg/day of loboob for 60 days. Blood samples were collected for hormone and antioxidant enzyme assays. Unbiased stereology was performed on testis tissues to evaluate the volume of different parts of the testis and the number of various testis cells. Data indicated that FSH, LH and MDA were increased, and testosterone, catalase, SOD were decreased in the busulfan group, while treatment with loboob at 70 and 140 mg/kg significantly improved these parameters (P <0.05). Treatment with 70 and 140 mg/kg of loboob ameliorated the germinal epithelium volume, types A and B spermatogonia, spermatocytes, elongated and round spermatids, and Sertoli cells in the seminiferous tubules (P <0.05). High concentration of loboob also improved testis weight and volume, and leydig cell number (P <0.05). Thus, loboob is more effective for the recovery of seminiferous tubules and their cells than for the interstitial tissue. Loboob with various antioxidants, minerals and vitamins could overcome the side effects of busulfan.
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ABSTRACT Purpose: COVID-19 continues to be an urgent World issue. Receptors of angiotensin converting enzyme 2 (ACE2), gateway of SARS-CoV-2, are present in the lungs, bladder, prostate, and testicles. Therefore, these organs face high risk of damage caused by the virus and this mechanism may explain non-respiratory symptoms of the disease. Materials and Methods: This systematic review, guided by the PRIMSA statement, was proposed to elucidate possible urological complications of COVID-19. Searches were carried out in Medline (PubMed), Cochrane (CENTRAL), Embase, MedRxiv and LILACS. Bias analysis was made using the specific Newcastle-Ottawa Scale for each study design. Results: Search was carried out until April 2022, and 8,477 articles were identified. Forty-nine of them were included in this systematic review. There is evidence that lower urinary tract symptoms and acute scrotum may be signs of COVID-19 in men, although in a small proportion. Also, the disease may have a transitory impact on male fertility, evidenced by several alterations in sperm counts. However, it must be clarified whether this impact is transitory, or may last for longer periods. Several patients showed reduction of total value of testosterone. Two authors linked low levels of testosterone with worse outcomes of COVID-19, suggesting that the hormone may be used as an early biomarker of the severity of the disease. Moreover, it is extremely unlikely that SARS-CoV-2 is transmitted by semen. Conclusion: This systematic review identified possible repercussions of COVID-19 in the urinary as well as in the male reproductive system.
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Oroxylum indicum (L.) Kurz, commonly called as Broken bones tree or Indian trumpet flower, belonging to Fam. Bignoniaceae, is traditionally used as a contraceptive by ethnic people of Tripura, North-East India. Here, we investigated the scientific basis for use of O. indicum as male antifertility agent by folklore healers. In vitro spermicidal activity of aqueous (AEOI) and methanolic (MEOI) extracts of O. indicum stem bark were studied on human sperm. The in vivo activity was experimented on male albino rats. The treated animals were allowed to mate and the pups delivered by female rat partners were counted. Phytochemical estimation of test samples was done using HPLC. The AEOI and MEOI treatments significantly decreased human sperm motility and viability. Test extracts have increased the hypo-osmotic swelling of sperm. Both the extracts were significantly declined the weight of reproductive organ. The MEOI treated rats have shown significant decrease in sperm motility and sperm counts. AEOI and MEOI treatment significantly reduced level of testosterone, but sharply raised dihydrotestosterone and prostaglandin in rats. Results testified the traditional claim for use of O. indicum as a male contraceptive agent, where MEOI have shown reversible action on male reproductive system leading to contraception without harming the libido.
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Benign prostatic hyperplasia (BPH) is an age-related non-neoplastic disease of the prostate gland in men that has become a global health issue in recent years. Due to the side effects of conventional treatment options, attention is now focused on phytotherapeutics for its management. We investigated the possible protective effect of Saccharomyces cerevisiae var. boulardii in a rat model of testosterone propionate (TP) induced BPH. Rats were divided into five groups: Gr. I, untreated control group; Gr. II, TP group; Gr. III, TP + finasteride; Gr. IV, TP + S. cerevisiae var. boulardii; and Gr. V, S. cerevisiae var. boulardii group. Treatments were given daily for 28 days. At the end of the experiment, all rats were weighed and the prostatic indices, prostate specific antigen, serum testosterone concentration as well as the histological and histomorphometric changes were evaluated. Saccharomyces cerevisiae var. boulardii significantly (P <0.05) reduced prostate weight, prostatic index, serum prostate specific antigen, prostatic epithelial thickness and increased luminal diameter. Thus, the results of this study suggest that S. cerevisiae var. boulardii is a potential pharmacological candidate for management of benign prostatic hyperplasia.
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abstract The purpose of the present study was to investigate the effect of two recovery periods between consecutive and non-consecutive days in strength training sessions on hormonal, neuromuscular and morphological variables in recreationally trained men. Nineteen young men completed the study and were randomly divided into two groups: 24R, 24-hour recovery group (n = nine) and 72R, 72-hour recovery group (n = ten). The strength training program (RT) lasted six weeks with two serial routines, with a weekly frequency of four times. The saliva sample was collected once a week in the morning to determine salivary testosterone. The 1RM, jump against movement and body composition tests were performed in the pre- and post-training periods. As for salivary testosterone, there was no significant effect with respect to time and between groups. Both groups improved maximal strength in terms of intervention time in the barbell bench press and in the leg press 45º, not differing between groups and body composition showed significant interaction in time to body fat percentage ∆% = -14.6 ± 10.0 (24R) and -17.2 ± 10.9 (72R); p = 0.00, fat mass ∆% = -13, 7 ± 9.2 (24R) and -18.2 ± 13.0 (72R); p = 0.00 and fat-free mass ∆% = 3.5 ± 2.7 (24R) and 2.5 ± 2 .8 (72R), p = 0.00. The recovery periods 24 and 72 hours between sessions induced similar responses in the parameters investigated in recreationally strength-trained men.
resumo O objetivo do presente estudo foi investigar o efeito de dois períodos de recuperação entre dias consecutivos e não consecutivos em sessões de treinamento de força sobre variáveis hormonais, neuromusculares e morfológicas em homens treinados recreacionalmente. Dezenove homens jovens completaram o estudo e foram divididos aleatoriamente em dois grupos: 24R, grupo de recuperação de 24 horas (n = nove) e 72R, grupo de recuperação de 72 horas (n = dez). O programa de treinamento de força (TF) durou seis semanas com duas rotinas seriadas, com frequência semanal de quatro vezes. A amostra de saliva foi coletada uma vez por semana no período da manhã para determinação da testosterona salivar. Os testes de 1RM, salto contra movimento e composição corporal foram realizados nos períodos pré e pós-treinamento. Quanto à testosterona salivar, não houve efeito significativo em relação ao tempo e entre os grupos. Ambos os grupos melhoraram a força máxima em termos de tempo de intervenção no supino reto com barra e no leg press 45º, não diferindo entre os grupos e composição corporal apresentaram interação significativa no tempo para percentual de gordura corporal ∆% = -14,6 ± 10,0 (24R) e -17,2 ± 10,9 (72R); p = 0,00, massa gorda ∆% = -13, 7 ± 9,2 (24R) e -18,2 ± 13,0 (72R); p = 0,00 e massa isenta de gordura ∆% = 3,5 ± 2,7 (24R) e 2,5 ± 2,8 (72R), p = 0,00. Os períodos de recuperação de 24 e 72 horas entre as sessões induziram respostas semelhantes nos parâmetros investigados em homens treinados de força recreacionalmente.
RESUMO
ABSTRACT Introduction: Testosterone is a steroid that can help with blood disorders, sexual dysfunctions, connective tissue diseases, some malignancies, intractable pain, and other serious diseases. However, it must be prescribed under medical supervision because of the risk of major adverse effects such as liver disease, heart disease, stroke, blood clots, and cancer. There is an urgent need for research on developing an electrochemical sensor to detect testosterone as a doping substance in sports. Objective: Develop an electrochemical sensor of poly(ionic liquid)-graphene oxide molecularly printed polymers (PIL/MIs/GO) to detect testosterone as a doping substance in sports. Methods: Morphological characterization of modified electrodes was performed by field emission scanning electron microscopy (FESEM), allowing the GO to be surface-mounted with fragments and apertures. Due to the holes generated by the agglomeration of PIL and MIs molecules on the wavy edges of the GO nanosheets, the surface morphology of PIL/MIs/GO/GCE also revealed a high porosity structure. Results: Compared to other synergistic influences of GO nanosheets with PIL and MIs molecules, electrochemical investigations using a differential pulse voltammetry approach indicated high selectivity, good stability, appropriate linear range, lower detection limit, and higher selectivity. Conclusion: In pharmaceutical samples and human biological fluids, the validity and accuracy of PIL/MIs/GO/GCE for the determination of testosterone demonstrated practical application. PIL/MIs/GO/GCE can thus be used as an accurate and reliable sensor for detecting testosterone as a doping agent in sports. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: A testosterona é um esteróide que pode ajudar com distúrbios sanguíneos, disfunções sexuais, doenças do tecido conjuntivo, algumas malignidades, dores intratáveis e outras doenças graves. No entanto, devido ao risco de grandes efeitos adversos como doenças hepáticas, doenças cardíacas, derrames, coágulos sanguíneos e câncer, ela deve ser prescrita sob supervisão médica. Há uma necessidade urgente da pesquisa sobre o desenvolvimento de um sensor eletroquímico para detectar a testosterona como substância dopante nos esportes. Objetivo: Desenvolver um sensor eletroquímico de poli-(líquido iônico)-polímeros impressos molecularmente em óxido de grafeno (PIL/MIs/GO) para detectar a testosterona como substância dopante nos esportes. Métodos: Efetuou-se a caracterização morfológica de eletrodos modificados por microscopia eletrônica de varredura de emissão de campo (FESEM) permitindo que o GO fosse em superfície com fragmentos e aberturas. Devido aos orifícios gerados pela aglomeração das moléculas de PIL e MIs nas bordas onduladas das nano folhas de GO, a morfologia da superfície de PIL/MIs/GO/GCE também revelou uma estrutura de alta porosidade. Resultados: Em comparação com outras influências sinergéticas das nanoquetas GO com as moléculas PIL e MIs, os resultados das investigações eletroquímicas utilizando a abordagem de voltametria de pulso diferencial indicaram alta seletividade, boa estabilidade, faixa linear apropriada, limite de detecção mais baixo e seletividade mais alta. Conclusão: Em amostras farmacêuticas e fluidos biológicos humanos, a validade e a precisão do PIL/MIs/GO/GCE para a determinação de testosterona demonstraram aplicação prática. O PIL/MIs/GO/GCE pode assim ser utilizado como um sensor preciso e confiável para a detecção de testosterona como agente dopante no esporte. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: La testosterona es un esteroide que puede ayudar en los trastornos sanguíneos, la disfunción sexual, las enfermedades del tejido conectivo, algunos tumores malignos, el dolor intratable y otras enfermedades graves. Sin embargo, debido al riesgo de que se produzcan efectos adversos importantes, como enfermedades hepáticas, cardíacas, accidentes cerebrovasculares, coágulos sanguíneos y cáncer, debe prescribirse bajo supervisión médica. Es urgente investigar el desarrollo de un sensor electroquímico para detectar la testosterona como sustancia dopante en el deporte. Objetivo: Desarrollar un sensor electroquímico de polímeros impresos molecularmente de poli(líquido iónico)-óxido de grafeno (PIL/MIs/GO) para detectar la testosterona como sustancia dopante en el deporte. Métodos: La caracterización morfológica de los electrodos modificados se llevó a cabo mediante microscopía electrónica de barrido de emisión de campo (FESEM) permitiendo que el GO estuviera en la superficie con fragmentos y aberturas. Debido a los agujeros generados por la aglomeración de moléculas de PIL y MIs en los bordes ondulados de las nanohojas de GO, la morfología superficial de PIL/MIs/GO/GCE también reveló una estructura de alta porosidad. Resultados: En comparación con otras influencias sinérgicas de las nanohojas de GO con las moléculas PIL y MIs, los resultados de las investigaciones electroquímicas utilizando el enfoque de la voltamperometría diferencial de impulsos indicaron una alta selectividad, una buena estabilidad, un rango lineal apropiado, un límite de detección más bajo y una mayor selectividad. Conclusión: En muestras farmacéuticas y fluidos biológicos humanos, la validez y precisión de PIL/MIs/GO/GCE para la determinación de testosterona demostró su aplicación práctica. Así pues, PIL/MIs/GO/GCE puede utilizarse como un sensor preciso y fiable para la detección de la testosterona como agente dopante en el deporte. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.