Study on improving the quality standard of Taohong Tongmai granule / 药学实践杂志

Objective To improve the quality standard of Taohong Tongmai granule.Methods TLC was used to make qualitative identification of Paeoniae Radix Rubra,Rehmanniae Radix,Glycyrrhizae Radix;The effective components of the main walnut kernel were identified by HPLC;and HPLC was used to do quantitative determination of Paeoniflorin,the determination was performed on Agilent HC-C18(250mm×4.6mm,5μm)column with mobile phase consisted of Acetonitrile-water(13:87) at the flow rate of 1.0 ml/min,the column temperature was 30 ℃ and the detection wavelength was set at 230 nm.Results These TLC spots were fairly clear,and the blank test showed no interference;The paeoniflorin at the range of 1·79-57.28 μg/ml was linear with peak area(r= 0.999 9),its average recovery rate was 99.99% and RSD was 2.05%(n= 9). Conclusion The quality standard of Taohong Tongmai granule had been improved,identification method was better reproduc-ibility,the determination method of paeoniflorin content had enhanced the controllability of product quality.

Pharmacokinetics of Tongmai granule in acute myocardialischemic model beagles / 中国中药杂志

Tongmai granule (TM) is composed of Puerariae Lobatae Radix (Gegen), Salviae Miltiorrhizae Radix et Rhizoma(Danshen) and Chuanxiong Rhizoma(Chuanxiong). It has been used to treat ischemic cardio-cerebrovascular diseases for decades. For the purpose of elucidating its pharmacodynamic material foundation, the absorption and pharmacokinetic property of TM were investigated in acute myocardial ischemic model beagles. All serum samples were extracted before analysis with ethyl acetate after being acidified by hydrochloric acid. Under negative ESI detection mode, the chromatographic separation was carried out with monolithic C₁₈ column for gradient elution. A simultaneous quantitative analysis was made on 15 polyphenols, including 8 from Gegen, 5 from Danshen and 2 from Chuanxiong, in 8.5 min. The validation result demonstrated the specificity, accuracy and precision of the method in line with the bioanalysis requirements. After TM solution was administrated to acute myocardial ischemic model beagles through duodenum injection, serum samples were collected after 6 h. The quantitative detection proved the prompt absorption of TM, all of the components were detectable in the blood samples 5 min later, and reached peak respectively at 0.18-3.83 h after administration. The components presented large variabilities. The most components were exposed in serum with puerarin and salvianic acid A, followed by 3'-methoxypuerarin, mirificin, and 3'-hydroxypuerarin. The study proves puerarin and salvianic acid A are dominating active components of TM in acute myocardial ischemic model beagles.

Simultaneous determination of five active components in Danxiong Tongmai Granule by HPLC / 中草药

Objective: To establish an HPLC method for the content determination of salvianolic acid B, fumalic acid, hydroxysafflor yellow A, gallic acid, and caffeic acid in Danxiong Tongmai Granule. Methods: The determination was carried out by HPLC, using Thermo ODS-2 Hypersil (250 mm×4.6 mm, 5 μm) chromatographic column. The mobile phase was 0.1% acetic acid solution-methyl alcohol, gradient elution, and UV detection wavelength was set at 286 nm. The column temperature was set at 30 ℃. The injection volume was 10 μL. Results: Under the above chromatographic conditions, there was a good linearity between the absorption peak area and the concentration in the ranges of 3.18-50.88 μg/mL (r=0.9990) for salvianolic acid B, 2.00-32.00 μg/mL (r=0.9995) for fumalic acid, 6.00-96.00 (r=0.9996) for hydroxysafflor yellow A, 0.60-9.60 μg/mL (r=0.9994) for gallic acid, and 1.00-16.00 μg/mL (r=0.9995) for caffeic acid, respectively. Conclusion: This method is simple, fast, accurate, reliable, and reproducible, and it is proper for the content determination of salvianolic acid B, fumalic acid, hydroxysafflor yellow A, gallic acid, and caffeic acid in Danxiong Tongmai Granule at the same time.

Preparation of ?-cyclodextrin Inclusion Compound of Volatile Oil from Danfu Tongmai Granules / 中药新药与临床药理

Objective To study the inclusion conditions for volatile oil from Danfu Tongmai Granules.Methods With the utilization rate of volatile oil and yield rate of inclusion as indexes,the optimum inclusion conditions were selected by orthogonal design,and the inclusion compound was identified by TLC.Results The optimal inclusion conditions for volatile oil were as follows:a proportion of 1 ∶7(mL ∶g) for oil to ?-cyclodextrin,stirring for 4 hours at 40 ℃.TLC results showed that inclusion process was successful and the main components of volatile oil were similar before and after inclusion.Conclusion This methods can be used for producing ?-cyclodextrin inclusion compound on a large scale.