RESUMO
SUMMARY: This study evaluated the phytochemical screening, antioxidant capacity, and in vitro anticancer activities of four plants namely, Gypsophila capillaris, Anabasis lachnantha, Haloxylon salicornicum, and Horwoodia dicksoniae which belong to four different families: Caryophyllaceae, Amaranthaceae, Chenopodiaceae, Brassicaceae, respectively. The total phenolics, anthocyanins, saponins, total antioxidant capacity (TAC), and DPPH assays were determined by spectrophotometer. In vitro anticancer activity was assessed using two human cancer cell lines; hepatocellular carcinoma (HepG-2) and breast adenocarcinoma (MCF-7) to estimate the inhibition concentration 50 % (IC50). The results showed that H. dicksoniae has the highest concentrations of phenolics and saponins, while H. salicornicum has the highest DPPH. The highest concentration of TAC was found in G. capillaries. Among the tested extracts, G. capillaries and H. salicornicum have the potential activity against MCF-7 and HepG-2 cell lines in vitro. The content of polyphenols in G. capillaries was profiled by high-performance liquid chromatography (HPLC). The highest concentration among the phenolic compounds was chlorogenic (60.8 µg/ml) while the highest concentration among the flavonoid compounds was hesperidin (1444.92 µg/ml). In summary, G. capillaries and H. salicornicum extracts have potent anticancer activity against HepG-2 and MCF-7 cell lines.
Este estudio evaluó la detección fitoquímica, la capacidad antioxidante y las actividades anticancerígenas in vitro de cuatro plantas, Gypsophila capillaris, Anabasis lachnantha, Haloxylon salicornicum y Horwoodia dicksoniae, que pertenecen a cuatro familias diferentes: Caryophyllaceae, Amaranthaceae, Chenopodiaceae y Brassicaceae, respectivamente. Los ensayos de fenólicos totales, antocianinas, saponinas, capacidad antioxidante total (TAC) y DPPH se determinaron mediante espectrofotómetro. La actividad anticancerígena in vitro se evaluó utilizando dos líneas celulares de cáncer humano; carcinoma hepatocelular (HepG-2) y adenocarcinoma de mama (MCF- 7) para estimar la concentración de inhibición del 50 % (IC50). Los resultados indicaron que H. dicksoniae tiene las concentraciones más altas de fenólicos y saponinas, mientras que H. salicornicum tiene el DPPH más alto. La mayor concentración de TAC se encontró en G. capillaries. Entre los extractos probados, G. capillaries y H. salicornicum tienen actividad potencial contra líneas celulares MCF-7 y HepG-2 in vitro. El contenido de polifenoles en G. capillaries se perfiló mediante cromatografía líquida de alta resolución (HPLC). La concentración más alta entre los compuestos fenólicos fue clorogénica (60,8 µg/ml), mientras que la concentración más alta entre los compuestos flavonoides fue la hesperidina (1444,92 µg/ml). En resumen, los extractos de Gypsophila capillaris y H. salicornicum tienen una potente actividad anticancerígena contra las líneas celulares HepG-2 y MCF-7.
Assuntos
Humanos , Plantas Medicinais/química , Antineoplásicos/química , Antioxidantes/química , Fenóis/análise , Saponinas/análise , Arábia Saudita , Técnicas In Vitro , Cromatografia Líquida de Alta Pressão , Metabolômica , Células Hep G2/efeitos dos fármacos , Células MCF-7/efeitos dos fármacos , Compostos Fitoquímicos , Antocianinas/análise , Antineoplásicos/farmacologia , Antioxidantes/farmacologiaRESUMO
Abstract Several reasons may underlie the dramatic increase in type2 diabetes mellitus. One of these reasons is the genetic basis and variations. Vitamin D receptor polymorphisms are associated with different diseases such as rheumatoid arthritis and diabetes. The aim of this study is to investigate the possible association of two identified mutations ApaI (rs7975232) and TaqI (rs731236). Eighty-nine healthy individuals and Fifty-six Type 2 Diabetic (T2D) patients were investigated using RFLP technique for genotyping and haplotyping as well. The distribution of Apal genotypes was not statistically significant among the control (P=0.65) as well as for diabetic patients (P=0.58). For Taql allele frequencies of T allele was 0.61 where of G allele was 0.39. The frequency distribution of Taql genotypes was not statistically significant among the control (P=0.26) as well as diabetic patients (P=0.17). Relative risk of the allele T of Apa1 gene is 1.28 and the odds ratio of the same allele is 1.53, while both estimates were < 1.0 of the allele G. Similarly, with the Taq1 gene the relative risk and the odds ratio values for the allele T are 1.09 and 1.27 respectively and both estimates of the allele C were 0.86 for the relative risk and 0.79 for the odds ratio. The pairwise linkage disequilibrium between the two SNPs Taq1/apa1 was statistically significant in control group (D = 0.218, D' = 0.925 and P value < 0.001) and similar data in diabetic groups (D = 0.2, D' = 0.875 and P value < 0.001). These data suggest that the T allele of both genes Apa1 and Taq1 is associated with the increased risk of type 2 diabetes. We think that we need a larger number of volunteers to reach a more accurate conclusion.
Resumo Várias razões podem estar subjacentes ao aumento dramático da diabetes mellitus tipo 2. Um desses motivos é a base genética e variações. Os polimorfismos do receptor da vitamina D estão associados a diferentes doenças, como artrite reumatoide e diabetes. O objetivo deste estudo é investigar a possível associação de duas mutações identificadas ApaI (rs7975232) e TaqI (rs731236). Oitenta e nove indivíduos saudáveis e 56 pacientes com diabetes tipo 2 (T2D) foram investigados usando a técnica RFLP para genotipagem e haplotipagem também. A distribuição dos genótipos Apal não foi estatisticamente significativa entre o controle (P = 0,65), bem como para os pacientes diabéticos (P = 0,58). Para as frequências do alelo Taql, o alelo T foi de 0,61, onde o alelo G foi de 0,39. A distribuição de frequência dos genótipos Taql não foi estatisticamente significativa entre o controle (P = 0,26), bem como os pacientes diabéticos (P = 0,17). O risco relativo do alelo T do gene Apa1 é 1,28 e a razão de chances do mesmo alelo é 1,53, enquanto ambas as estimativas foram < 1,0 do alelo G. Da mesma forma, com o gene Taq1, os valores de risco relativo e razão de chances para o alelo T são 1,09 e 1,27, respectivamente, e ambas as estimativas do alelo C foram de 0,86 para o risco relativo e 0,79 para o odds ratio. O desequilíbrio de ligação par a par entre os dois SNPs Taq1 / apa1 foi estatisticamente significativo no grupo de controle (D = 0,218, D' = 0,925 e valor P < 0,001) e dados semelhantes em grupos diabéticos (D = 0,2, D' = 0,875 e valor P < 0,001). Esses dados sugerem que o alelo T de ambos os genes Apa1 e Taq1 está associado ao aumento do risco de diabetes tipo 2. Achamos que precisamos de um número maior de voluntários para chegar a uma conclusão mais precisa.
Assuntos
Humanos , Receptores de Calcitriol/genética , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/epidemiologia , Arábia Saudita , Estudos de Casos e Controles , Polimorfismo de Nucleotídeo Único , Frequência do Gene , GenótipoRESUMO
Abstract The presence of weeds in areas of agricultural activities is a hinderance to the development of these activities. It is important to take advantage of the vast open spaces suitable for agriculture and provide food security for humans, and also it is an important indicator for determining the feasibility of growing crops, benefiting from yield and determining the percentage of loss, clearing fields through agricultural practices, that protect crops from weed attack and agricultural practice method must be followed that will reduce weed presence. This study was conducted during the years 2018 to 2020 to evaluate Portulacaceae of Flora in the Taif area in the Kingdom of Saudi Arabia at different altitudes (Area 1 =1700 m, Area 2 =1500 m, Area 3 =1500 m, Area 4 =500 m ِ Area 5 = 2200 m, and Area 6 = 2200 m). The results show that there were 2,816 individuals of Portulaca oleracea weed, with the highest density found in A 1, followed by A 2, while in A 5 and A 6, no weeds were recorded. The highest density of weeds were in the Pomegranate fields, followed by Grape fields. The lowest density was found in A man field. The results of this study will help to take the necessary measures to combat weeds and its management in areas of agricultural activity, while more studies are needed to survey the ecology of weeds of Taif in The Kingdom of Saudi Arabia.
Resumo A presença de plantas daninhas em áreas de atividades agrícolas é um entrave ao desenvolvimento dessas atividades. É importante aproveitar os vastos espaços abertos adequados para a agricultura e dar segurança alimentar para o homem. Também é um indicador importante para determinar a viabilidade de cultivo de lavouras, beneficiando-se da produtividade e determinando o percentual de perda, desmatando campos agrícolas, práticas que protegem as lavouras do ataque de ervas daninhas, e métodos de práticas agrícolas devem ser seguidos para reduzir a presença de ervas daninhas. Este estudo foi realizado durante os anos de 2018 a 2020 para avaliar Portulacaceae de flora na área de Taif, no Reino da Arábia Saudita, em diferentes altitudes (Área 1 = 1.700 m, Área 2 = 1.500 m, Área 3 = 1.500 m, Área 4 = 500 m, Área 5 = 2.200 m, e Área 6 = 2.200 m). Os resultados mostram que houve 2.816 indivíduos de planta daninha Portulaca oleracea, com a maior densidade encontrada em A 1, seguida de A 2, enquanto em A 5 e A 6, nas plantas daninhas foram registrados. A maior densidade de ervas daninhas estava nos campos de romã, seguido pelos campos de uva. A densidade mais baixa foi encontrada no campo A man. Os resultados deste estudo ajudarão a tomar as medidas necessárias para combater as ervas daninhas e seu manejo em áreas de atividade agrícola, enquanto mais estudos são necessários para levantar a ecologia das ervas daninhas de Taif na Arábia Saudita.
Assuntos
Humanos , Portulacaceae , Arábia Saudita , Produtos Agrícolas , Agricultura , Plantas DaninhasRESUMO
Agriculture sector of Saudi Arabia is growing swiftly and tomato is an important crop cultivated mostly under green houses. Unfortunately, it is facing severe infestation due to divers mite species. Present study, relates to evaluation of toxicity of oxamyl against two phytophagous mites; Aculops lycopersici and Tetranychus urticae, isolated from tomato plants suffering from infestation. Simultaneous effect of oxamyl on two predatory mites; Neosiulus cucumeris and Euseius scutalis, was also evaluated. Three concentrations of oxamyl; half of the recommended dose (HRD), recommended dose (RD) and double recommended dose (DRD), were used against each mite specie to observe mortality within seven days as compared to the control. Significant mortality of 97.91% and 93.92% was observed in A. lycopersici and T. urticae, respectively at RD. In case of predatory mites; N. cucumeris and E. scutalis, mortality was 60.61% and 64.48%, respectively, under same conditions. Mortality of mites observed at HRD was insignificant and there was negligible increase in mortality at DRD. Oxamyl being less toxic to predatory mites and significantly mortal to phytophagous mites is recommended as a tool to as a tool to achieve biological control parallel to pesticidal effect.
O setor agrícola da Arábia Saudita está crescendo rapidamente e o tomate é uma cultura importante cultivada principalmente em estufas. Infelizmente, está enfrentando uma infestação severa devido a diversas espécies de ácaros. O presente estudo refere-se à avaliação da toxicidade do oxamil contra dois ácaros fitófagos; Aculops lycopersici e Tetranychus urticae, isolados de tomateiros infestados. Efeito simultâneo de oxamil em dois ácaros predadores; Neosiulus cucumeris e Euseius scutalis, também foi avaliado. Três concentrações de oxamil; metade da dose recomendada (HRD), dose recomendada (RD) e dose dupla recomendada (DRD), foram usados ââcontra cada espécie de ácaro para observar a mortalidade em sete dias em comparação com o controle. Mortalidade significativa de 97,91% e 93,92% foi observada em A. lycopersici e T. urticae, respectivamente no RD. No caso de ácaros predadores; N. cucumeris e E. scutalis, a mortalidade foi de 60,61% e 64,48%, respectivamente, nas mesmas condições. A mortalidade de ácaros observada no HRD foi insignificante e houve um aumento insignificante na mortalidade no DRD. Oxamil sendo menos tóxico para ácaros predadores e significativamente mortal para ácaros fitófagos é recomendado como ferramenta para alcançar o controle biológico paralelo ao efeito pesticida.
Assuntos
Animais , Controle Biológico de Vetores , Solanum lycopersicum , Agricultura , Ácaros , Arábia SauditaRESUMO
SUMMARY: Anatomy is a three-dimensional subject which needs face-to-face interaction and faced major challenges during the pandemic warranted changes in medical education. A narrative review was conducted to assess the attitude and perception of students and teaching faculty of healthcare professional courses towards Anatomy online teaching in the Kingdom of Saudi Arabia (KSA). A five stage framework narrative reviews outlined by Arksey & O'Malley (2005) was adopted for the current study. Inclusion criteria was studies conducted in Saudi Arabian healthcare professional colleges regarding online Anatomy teaching during the pandemic from March 2020 to April 2023. PRISMA-ScR search strategy was employed for identifying relevant studies which were managed using Endnote reference manager version 20. Nine articles were included in the review out of which two were mixed method studies, two were qualitative and five were quantitative studies. Students and teachers were mostly found to be satisfactory of the Anatomy e-learning experience. Major setbacks found were student and teaching faculty's negative attitude towards online practical teaching and concerns regarding academic dishonesty in students during online examination. Role of digital proficiency of teachers and students in the success of e-learning was emphasized by the review. This narrative review has mapped out the strengths and gaps in remote Anatomy teaching in Saudi Arabia during COVID-19 pandemic. There is a generally successful transition to e-learning in KSA from conventional teaching in Anatomy during the pandemic notwithstanding a few challenges that need to be addressed to improve the teaching and learning experience of Anatomy. Keywords: Anatomy, Online, E-learning, Saudi Arabia.
La anatomía es un tema tridimensional que necesita una interacción cara a cara y enfrentó grandes desafíos durante la pandemia, que justificó cambios en la educación médica. Se realizó una revisión narrativa para evaluar la actitud y la percepción de los estudiantes y profesores de cursos para profesionales de la salud, hacia la enseñanza en línea de Anatomía en el Reino de Arabia Saudita. Para el estudio actual, se adoptó una revisión narrativa del marco de cinco etapas delineada por Arksey & O'Malley (2005). Los criterios de inclusión fueron estudios realizados en colegios profesionales del área de la salud de Arabia Saudita, con respecto a la enseñanza de anatomía en línea durante la pandemia desde marzo de 2020 hasta abril de 2023. Se empleó la estrategia de búsqueda PRISMA-ScR para identificar estudios relevantes que se administraron utilizando el administrador de referencia Endnote versión 20. Se incluyeron nueve artículos en la revisión, de los cuales dos eran estudios de métodos mixtos, dos eran estudios cualitativos y cinco eran estudios cuantitativos. Se encontró que los estudiantes y profesores, en su mayoría estaban satisfechos con la experiencia de aprendizaje electrónico de Anatomía. Los principales contratiempos encontrados fueron la actitud negativa de los estudiantes y profesores hacia la enseñanza práctica en línea y las preocupaciones sobre la deshonestidad académica de los estudiantes durante los exámenes en línea. La revisión enfatizó el papel de la competencia digital de profesores y estudiantes en el éxito del aprendizaje electrónico. Esta revisión narrativa ha mapeado las fortalezas y brechas en la enseñanza remota de anatomía en Arabia Saudita, durante la pandemia de COVID-19. En general, hay una transición exitosa al aprendizaje electrónico en Arabia Saudita desde la enseñanza convencional de Anatomía durante la pandemia, a pesar de algunos desafíos que deben abordarse para mejorar la experiencia de enseñanza y aprendizaje de Anatomía.
Assuntos
Humanos , Educação a Distância , COVID-19 , Anatomia/educação , Arábia Saudita , PandemiasRESUMO
Aim: To assess the distance between the inferior alveolar canal and the roots of the mandibular second molar and the mandibula and cortex in a Saudi Arabian subpopulation through existing CBCT images. Materials and Methods: This retrospective study was performed based on 120 patients CBCT images in five age groups.The distances (D1 and D2) between the buccal cortex (BC), lingual cortex (LC), and mandibular molars and the distances (D3) between the root apices and inferior alveolar nerve canal (IANC) were measured for each dental root on the right and left of the mandible with the help of Vision iCAT software. A radiology specialist with a gap of 15 days twice carried out the measurements. Statistical analysis was carried out with the help of SPSS 24. to analyse variability Chi-square analysis was done, and the p value was fixed at > 0.05. To check inter-person variability, Cohen's variability was fixed at 0.8. Results: The distance between the outer surface of the buccal cortical plate and the buccal root surface ranged between 3.8 and 5.7 mm, whereas the distance between the root apices of the mandibular molars and the IANC ranged between 4.8 and 3.5 mm. The distance from the outer surface of the lingual cortical plate to the lingual root surface varied between 1.2 and 2.8 mm. The mean distance between the root apices and IANC increased with age, more so in males than females. Conclusions: Even though this study was conducted on a small sample size, it will help the dental practitioners in planning endodontic procedures, surgical extractions, and implant placements, and it should be repeated with a higher number of images.
Objetivo: Evaluar la distancia entre el canal alveolar inferior y las raíces del segundo molar mandibular, y la mandíbula y la corteza en una subpoblación de Arabia Saudita a través de imágenes CBCT existentes.Materiales y Métodos: Este estudio retrospectivo se realizó con base en imágenes CBCT de 120 pacientes en cinco grupos de edad. Las distancias (D1 y D2) entre la corteza bucal (BC), la corteza lingual (LC) y los molares mandibulares y las distancias (D3) entre los Se midieron los ápices radiculares y el canal del nervio alveolar inferior (IANC) para cada raíz dental a la derecha e izquierda de la mandíbula con la ayuda del software Vision iCAT. Un especialista en radiología, con un intervalo de 15 días, realizó dos veces las mediciones. El análisis estadístico se realizó con la ayuda del SPSS 24. Para analizar la variabilidad se realizó un análisis de Chi-cuadrado y el valor p se fijó en > 0,05. Para comprobar la variabilidad entre personas, la variabilidad de Cohen se fijó en 0,8. Resultados: La distancia entre la superficie exterior de la placa cortical bucal y la superficie de la raíz bucal osciló entre 3,8 y 5,7 mm, mientras que la distancia entre los ápices radiculares de los molares mandibulares y el IANC osciló entre 4,8 y 3,5 mm. La distancia desde la superficie exterior de la placa cortical lingual hasta la superficie de la raíz lingual varió entre 1,2 y 2,8 mm. La distancia media entre los ápices de las raíces y la IANC aumentó con la edad, más en hombres que en mujeres. Conclusión: Aunque este estudio se realizó con un tamaño de muestra pequeño, ayudará a los odontólogos a planificar procedimientos de endodoncia, extracciones quirúrgicas y colocación de implantes, y debe realizarse con más números.
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Raiz Dentária/anatomia & histologia , Processo Alveolar/anatomia & histologia , Mandíbula/anatomia & histologia , Dente Molar/anatomia & histologia , Arábia Saudita , Raiz Dentária/diagnóstico por imagem , Epidemiologia Descritiva , Tomografia Computadorizada de Feixe Cônico , Processo Alveolar/diagnóstico por imagem , Mandíbula/diagnóstico por imagem , Nervo Mandibular , Dente Molar/diagnóstico por imagemRESUMO
Grewia tenax (Forssk.) Fiori (Malvaceae) grows in the Arabian Peninsula and is used for several medicinal purposes. To characterize the dermatological bioactivities of G. tenax in terms of its antimelanoma, antityrosinase and antioxidant activities. Cytotoxicity was assessed by cell proliferation and mitochondrial viability assays. Ability to inhibit mushroom tyrosinase and scavenge free radicals were evaluated by an enzymatic and DPPH scavenging microtiter assay, respectively. Phytochemical analyses were carried out using TLC, HPLC-UV and NMR. The chloroform extract shown significant cytotoxic activity in terms of mitochondrial viability (43 ± 14 µg/mL). We identified lupeol and b-sitosterol as the main active components for the tyrosinase inhibitory activity of the hexane extract. Scavenging activity of the DPPH· radical was confined to the water extract. Extracts from this plant have the potential to be used as a base in the development of cosmeceutical products intended to whiten skin or to combat radical-induced physiopathological processes.
Grewia tenax (Forssk.) Fiori (Malvaceae) crece en la Península Arábiga y se utiliza con varios fines medicinales. Para caracterizar las bioactividades dermatológicas de G. tenax en cuanto a sus actividades antimelanoma, antitirosinasa y antioxidante; la citotoxicidad se evaluó mediante ensayos de proliferación celular y viabilidad mitocondrial. La capacidad para inhibir la tirosinasa de hongo y eliminar los radicales libres se evaluó mediante un ensayo de microtitulación enzimático y de eliminación de DPPH, respectivamente. Los análisis fitoquímicos se realizaron mediante TLC, HPLC-UV y NMR. El extracto de cloroformo mostró una actividad citotóxica significativa en términos de viabilidad mitocondrial (43 ± 14 µg/mL). Identificamos lupeol y b-sitosterol como los principales componentes activos para la actividad inhibitoria de tirosinasa del extracto de hexano. La actividad depuradora del radical DPPH· se limitó al extracto acuoso. Los extractos de esta planta tienen potencial para ser utilizados como base en el desarrollo de productos cosmecéuticos destinados a blanquear la piel o combatir procesos fisiopatológicos inducidos por radicales.
Assuntos
Citotoxinas/química , Grewia/química , Arábia Saudita , Técnicas In Vitro , Monofenol Mono-Oxigenase/farmacologia , Melanoma/terapia , Antioxidantes/farmacologiaRESUMO
Abstract Serum toxic metals have been implicated in development of many diseases. This study investigated the association between blood levels of lead and cadmium with abnormal bone mineral density (BMD) and incidence of osteoporosis. Sixty Saudi male adults age matching were assigned into two groups: A healthy control group (n = 30) and osteoporosis patients diagnosed according to T-score (n = 30). Serum calcium, vitamin D, osteocalcin, lead, cadmium were measured. Osteoporotic group showed a highly significant elevation of blood lead and cadmium levels compared to the control group (p <0.001). BMD was negatively correlated with serum osteocalcin level compared with control. There was a significant negative correlation between the cadmium and lead levels (r=-0.465 and p-value = 0.01) and calcium (p < 0.004). Our findings suggested that high cadmium and lead were negative correlated to BMD and increased the risk factor for osteoporosis.
Resumo Os metais tóxicos do soro têm sido implicados no desenvolvimento de muitas doenças. Este estudo investigou a associação entre os níveis sanguíneos de chumbo e cádmio com densidade mineral óssea anormal (DMO) e incidência de osteoporose. Sessenta adultos sauditas do sexo masculino com idades iguais foram divididos em dois grupos: um grupo de controle saudável (n = 30) e pacientes com osteoporose diagnosticados de acordo com o T-score (n = 30). Cálcio sérico, vitamina D, osteocalcina, chumbo, cádmio foram medidos. O grupo osteoporótico apresentou elevação altamente significativa dos níveis de chumbo e cádmio no sangue em comparação ao grupo controle (p < 0,001). A DMO foi negativamente correlacionada com o nível de osteocalcina sérica em comparação com o controle. Houve correlação negativa significativa entre os níveis de cádmio e chumbo (r = -0,465 ep = 0,01) e cálcio (p < 0,004). Nossos achados sugeriram que cádmio e chumbo elevados foram correlacionados negativamente à DMO e aumentaram o fator de risco para osteoporose.
Assuntos
Humanos , Masculino , Adulto , Osteoporose/epidemiologia , Chumbo , Arábia Saudita/epidemiologia , Absorciometria de Fóton , Osteocalcina , IncidênciaRESUMO
Abstract Seaweeds are a major marine resource that can be explored to develop novel pharmaceutical molecules. The present study showed the presence of unique bioactive components in the petroleum ether extract (PEE) and methanolic extract (ME) of Sargassum tenerrimum. The gas chromatography-mass spectrometry analysis suggested that the PEE of S. tenerrimum contained antibacterial biomolecules: hexadecanoic acid, methyl ester, 17-pentatriacontene, dasycarpidan-1-methanol, and acetate (ester). However, the ME of S. tenerrimum exhibited better antibacterial effect than the PEE due to the presence of the bioactive compounds 1,2-benzenedicarboxylic acid, diisooctyl ester, tetratetracontane, 1-docosene, 1,2-benzenediol, and benzoic acid. Thus, promising antibacterial molecules can be isolated from S. tenerrimum for better therapeutic use.
Resumo As algas marinhas são um importante recurso marinho que pode ser explorado para desenvolver novas moléculas farmacêuticas. O presente estudo mostrou a presença de componentes bioativos únicos no extrato etéreo de petróleo (PEE) e no extrato metanólico (ME) de Sargassum tenerrimum. A análise por cromatografia gasosa-espectrometria de massa sugeriu que o PEE de S. tenerrimum continha biomoléculas antibacterianas: ácido hexadecanoico, éster metílico, 17-pentatriaconteno, dasycarpidan-1-metanol e acetato (éster). Entretanto, o ME de S. tenerrimum exibiu melhor efeito antibacteriano do que o PEE devido à presença dos compostos bioativos ácido 1,2-benzenodicarboxílico, éster diisooctil, tetratetracontano, 1-docosene, 1,2-benzoenodiol e ácido benzoico. Assim, moléculas antibacterianas promissoras podem ser isoladas de S. tenerrimum para melhor uso terapêutico.
Assuntos
Sargassum , Arábia Saudita , Extratos Vegetais , Oceano Índico , Antibacterianos/farmacologiaRESUMO
Abstract The objective of the present study was to analyse the bioactive compounds of the leaves of Conocarpus lancifolius (C. lancifolius). The GC-MS analysis of the hot methanolic extract of the leaves (HMEL) of C. lancifolius exhibited the bioactive compounds such as 1-(3-Methoxy-2-nitrobenzyl) iso quinoline, morphin-4-ol-6,7-dione, 1-bromo-N-methyl-, phytol, hexadecanoic acid, 2,3-dihydroxypropyl ester, 2,2':4',2"-terthiophene, ethyl iso-allocholate, caryophyllene oxide, campesterol, epiglobulol, cholestan-3-ol, 2-methylene-, (3á,5à)-, dasycarpidan-1-methanol, acetate (ester) and oleic acid, eicosyl ester. The FT-IR analysis of HMEL of C. lancifolius showed a unique peak at 3184, 2413, 1657 cm-1 representing coumaric acid, chlorogenic acid and ferulic acid. The HMEL of C. lancifolius was actively inhibiting the proliferation of breast cancer cells MCF-7 ATCC at the concentration of 72.66 ± 8.21 µg/ml as IC50 value. The HMEL of C. lancifolius also revealed a good spectrum of activity against Gram-positive and Gram-negative bacterial cultures screened in this work. The activity observed has shown more or less similar effects against screened bacteria. However, the magnitude of potentiality was significantly lesser compared to standard ciprofloxacin disc at p< 0.001 level (99% confidence intervals). Furthermore, the study demonstrating the bioactive compounds can be isolated from the leaves of C. lancifolius.
Resumo O objetivo do presente estudo foi analisar os compostos bioativos das folhas de Conocarpus lancifolius (C. lancifolius). A análise por GC-MS do extrato metanólico quente das folhas (HMEL) de C. lancifolius exibiu os compostos bioativos como 1- (3-Metoxi-2-nitrobenzil) isoquinolina, morfina-4-ol-6,7- diona, 1-bromo-N-metil-, fitol, ácido hexadecanoico, 2,3-di-hidroxipropil éster, 2,2 ': 4', 2 " - tertiofeno, isoalocolato de etil, óxido de cariofileno, campesterol, epiglobulol, colestano -3-ol, 2-metileno-, (3á, 5à) -, dasycarpidan-1-metanol, acetato (éster) e ácido oleico, éster eicosílico. A análise FT-IR de HMEL de C. lancifolius mostrou um pico único em 3184, 2413, 1657 cm-1 representando ácido cumarico, ácido clorogênico e ácido ferúlico. O HMEL de C. lancifolius inibiu ativamente a proliferação de células de câncer de mama MCF-7 ATCC na concentração de 72,66 ± 8,21 µg / ml como valor de IC50. O HMEL de C. lancifolius também revelou bom espectro de atividade contra culturas de bactérias Gram-positivas e Gram-negativas rastreadas neste trabalho. A atividade observada mostrou efeitos mais ou menos semelhantes contra bactérias rastreadas. No entanto, a magnitude da potencialidade foi significativamente menor em comparação com o disco de ciprofloxacina padrão em nível de p < 0,001 (intervalos de confiança de 99%). Além disso, o estudo demonstrando os compostos bioativos pode ser isolado das folhas de C. lancifolius.
Assuntos
Árvores , Folhas de Planta , Arábia Saudita , Extratos Vegetais/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Antibacterianos/farmacologiaRESUMO
The speedy change in the Saudi Arabian community's socio-demographic pattern will significantly influence reproductive attitudes and practices with increasing preferences toward family planning because of the use of contraceptives. The current study was conducted to determine the attitudes and knowledge of married women in the Aseer region of Saudi Arabia regarding contraceptives use. Saudi married women from the Aseer region were the participants of this cross-sectional study. The study's objectives were covered via a standardized questionnaire, and the study comprised of 412 married women. A 100 % participant's response was demonstrated, while 31.8 % of the respondents were 31-40 years old. Most of the participants have a great awareness and knowledge about contraceptives, while (n=324; 78.6%) had previously used contraceptives. Additionally, 297 (72.1%) have intention to use contraceptive methods in the future. Majority of the participants (n=297; 91.6%) considered the economic and family planning as a reason for using the contraceptives, while natural family planning was mostly preferred (n=202; 49%). Logistic regression analysis exhibited significant correlation between the age, education, employment, monthly income and children number. The findings show that Saudi married women have high perceptions and knowledge of contraception. However, more effort is required to raise awareness regarding family planning and contraceptives, whereas the policy makers must exclude the obstacles to women from using contraceptives.
Assuntos
Feminino , Arábia Saudita , Cônjuges , Anticoncepção , Planejamento FamiliarRESUMO
Abstract: In forensic and legal contexts, an assessment of skeletal maturation is required to predict the chronological age in certain circumstances in which the chronological age is undocumented or unable to be proven. Carrying such medical examinations will always pose an ethical concern, especially in cases of assessing sexual maturity as well as examinations involving ionizing radiation. The cultural and religious barriers are also discussed concerning the ethical standards set in Saudi Arabia. Several ethical issues concerning age estimation examinations including issues related to consent forms, privacy, and radiation hazards have been documented. While ethical issues regarding radiation hazards are more likely to be overcome by introducing other imaging modalities without radiation hazards, ethics related to consent forms and privacy are still to be improved.
Resumen: En contextos forenses y legales, se requiere la evaluación de la maduración del esqueleto para predecir la edad cronológica en ciertas circunstancias en que la edad cronológica no está documentada o no es posible de probar. Realizar tales exámenes médicos siempre significa una preocupación ética, especialmente en casos de evaluación de madurez sexual así como en exámenes que involucran radiaciones ionizantes. Se discuten también las barreras culturales y religiosas relacionadas con el contexto de estándares éticos en Arabia Saudita. Entre los varios temas éticos relacionados con la estimación de la edad, se documentan temas relacionados con los formularios de consentimiento, privacidad y riesgos de radiación. Mientras que los temas éticos relacionados con riesgos de radiación pueden ser resueltos al introducir otras modalidades de examen sin radiación, los problemas éticos relacionados con los formularios de consentimiento y privacidad todavía necesitan mejorarse.
Resumo: Em contextos forenses e legais, uma avaliação de maturação esquelética é requerida para predizer a idade cronológica em certas circunstâncias nas quais a idade cronológica não é documentada ou impossível de ser provada. Conduzir tais exames médicos sempre irá suscitar uma preocupação ética, especialmente em casos para avaliar maturidade sexual bem como exames envolvendo radiação ionizante. As barreiras culturais e religiosas também são discutidas no que diz respeito a padrões éticos estabelecidos na Arábia Saudita. Diversos aspectos éticos concernentes a exames de estimativa de idade incluindo aspectos relacionados a formulários de consentimento, privacidade e risco de radiação foram documentados. Ao passo que aspectos éticos que dizem respeito a risco de radiação serem mais provavelmente superáveis pela introdução de outras modalidades de exames de imagem sem risco de radiação, a ética relacionada a formulários de consentimento e privacidade ainda estão por serem melhorados.
Assuntos
Humanos , Determinação da Idade pelo Esqueleto/ética , Riscos de Radiação , Medicina Legal/ética , Arábia Saudita , Privacidade , Consentimento Livre e EsclarecidoRESUMO
Abstract Dysmenorrhea is a common condition among females that is characterized by painful cramps before or during menstruation. It is considered as a common gynecological complaint that affects the quality of women's life. The study evaluated prevalence of dysmenorrhea, its impact, associated risk factors, and the management strategies adopted by female university students in Taif city, Saudi Arabia. A cross-sectional study was conducted among 562 female students aged 18-30 years at the university level. The results showed a high prevalence rate of dysmenorrhea (79.4%) among the students. The most common risk factors were family history (87.4%) and length of menstruation (79%). Half (50.2%) of the respondents were absent at the university at least 1 day every month. The most widely used medications by the respondents were ibuprofen (42%) and paracetamol (40%), whereas only 3% used mefenamic acid, despite that they experienced complete pain relief with mefenamic acid. High prevalence rate of dysmenorrhea associated with risk factors such as family history and length of menstruation, was found among university students. However, pain and associated symptoms affect the quality of life.
Assuntos
Humanos , Feminino , Adulto , Estudantes/classificação , Mulheres , Estudos Transversais/instrumentação , Dismenorreia/patologia , Menstruação/metabolismo , Dor/tratamento farmacológico , Qualidade de Vida , Arábia Saudita/etnologia , Universidades , Inquéritos e Questionários/estatística & dados numéricosRESUMO
Abstract Healthcare professionals use a variety of drug information sources to fulfill their clinical needs and medical practice. The aim of present study was to assess the sources of drug information among hospital' prescribers and evaluate their prescribing behavior in Saudi hospitals. A cross-sectional survey was conducted among randomly selected hospital' prescribers using a self-administered questionnaire. The response rate to the survey was 64.29%, with a ratio of 76.44% male and 23.56% female. The internet 137(60.89%) and textbooks 86(38.22%) were the prevalent sources for drug information used. Up-To-Date 107(47.56%), Medscape 105(46.67%) and FDA 74(32.88%) were the common electronic drug sources used. About 151(67.11%) of hospital' prescribers considered the pharmacist as a reliable drug information source. The most favored drug requests by hospital' prescribers from the pharmacists were drug alternatives 110(48.89%) followed by drug interactions 94(41.78%), side effects 78(34.67%) and indications 60(26.67%). Therapeutic efficacy 168(74.67%) and drug availability 73(32.44%) were the main factors contributed to the selection of drugs. This study shows some differences in hospital prescribers' perceptions of sources of drug information depending upon their background and clinical practice. Therefore, knowing appropriate drug information used by hospital' prescribers is fundamental for drug efficacy and safety in clinical practice.
Assuntos
Farmacêuticos/ética , Atitude , Inquéritos e Questionários , Necessidades e Demandas de Serviços de Saúde , Arábia Saudita/etnologia , Comportamento/ética , Preparações Farmacêuticas/normas , Atenção à Saúde/normas , Medicamentos sob Prescrição/análise , Prescrições/classificação , Hospitais/normasRESUMO
Abstract Objective: To evaluate root canal configuration and morphology of premolar teeth among Saudi subpopulations using cone beam computed tomography (CBCT). Material and Methods: In this retrospective cross-sectional study, CBCT images of 314 patients comprising 346 maxillary and 412 mandibular first premolar (FPM) teeth, 298 maxillary and 387 mandibular second premolar (SPM) teeth were analyzed to evaluate the number of roots, root canal morphology, and configuration based on the Vertucci's classification. The average intra-class correlation coefficient value was 0.931. Results: In the maxillary first premolar, 52.6% were two separate rooted and single rooted teeth, with one canal in 81.2% of the maxillary second premolar. Among the mandibular FPM, 96.6% of the teeth had one root and canal, and 97.9% of mandibular SPM had one root and canal. Type 1 canal configuration was seen as most common in all premolars. The number of roots in mandibular premolars did not reveal the difference among gender. Conclusion: Wide variations in root canal morphology and canal configuration system exists among maxillary and mandibular premolar teeth (AU).
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Dente Pré-Molar , Cavidade Pulpar/anatomia & histologia , Tomografia Computadorizada de Feixe Cônico , Arábia Saudita/epidemiologia , Distribuição de Qui-Quadrado , Estudos Transversais/métodos , Estudos RetrospectivosRESUMO
Abstract Objective: To assess the knowledge of pregnant women regarding their child's oral health. Material and Methods: A questionnaire was developed and the 1st section comprised sociodemographic variables. The second section contained questions about the eruption of teeth, fluoride importance, cariogenic food, and dental visits. The questionnaire was shared electronically via a link to the receptionist of the gynaecologist at different health centres of Najran to be filled by expectant mothers. The convenient sampling method was used to collect the responses. Data were presented using descriptive statistics. Results: A total of 572 pregnant women participated in this survey. Three hundred and fifteen (55.1%) knew that 1st primary tooth erupts at the age of 6 months. The majority of the respondents (n=332) agreed that toothbrushes and toothpaste could be used to clean a child's teeth; only 5.4%, 10%, and 24.5% preferred miswak, mouthwash, and toothbrush, respectively. Participants were well familiar with cariogenic food and occasionally allowed their children to take it. They have enough knowledge about fluoride toothpaste, but they were not familiar with the benefits of fluoride varnish. Almost 50% of the respondents agreed that the child should visit the dentist within six months, and 27.4% said they should visit the dentist whenever there is a problem. Conclusion: Almost 50% of participants showed a positive attitude towards most questions. However, there is a need to improve their behaviour and knowledge about many aspects of dental care.
Assuntos
Humanos , Feminino , Gravidez , Arábia Saudita/epidemiologia , Conhecimentos, Atitudes e Prática em Saúde , Saúde Bucal/educação , Assistência Odontológica , Odontopediatria , Gestantes , Dente Decíduo , Erupção Dentária , Estudos Transversais/métodos , Inquéritos e QuestionáriosRESUMO
Due to the urbanization and human invasion of the natural environments, great changes have been occurred on the food composition and feeding ecology of several animals especially those are sharing human his habitat in fields, wadis and gardens. The desert hedgehogs Paraechinus aethiopicus populations inhabiting different localities in Saudi Arabia were studied by using stomach contents analysis between February 2015 and October 2019. Precise analysis of stomach contents of 55 hedgehogs showed that the food of P. aethiopicus is highly diverse and highly influenced with effect of human on the environment including cooked rice, insects, plant materials, eggshells, worms, garbage and remnants of mammals and birds. Diet composition showed seasonal variations that are apparently associated with changes in the availability of different food items. The present results clearly showed that P. aethiopicus is an omnivorous mammal, capable of adapting to a great variety of dietary compositions in the study sites.
Devido à urbanização e invasão humana dos ambientes naturais, grandes mudanças têm ocorrido na composição alimentar e ecologia alimentar de vários animais, especialmente aqueles que estão compartilhando seu hábitat humano em campos, wadis e jardins. As populações de ouriços-do-deserto Paraechinus aethiopicus que habitam diferentes localidades na Arábia Saudita foram estudadas usando análise de conteúdo estomacal entre fevereiro de 2015 e outubro de 2019. A análise precisa do conteúdo estomacal de 55 ouriços mostrou que a alimentação de P. aethiopicus é altamente diversa e altamente influenciada com efeito de humanos no meio ambiente, incluindo arroz cozido, insetos, materiais vegetais, cascas de ovo, vermes, lixo e restos de mamíferos e pássaros. A composição da dieta apresentou variações sazonais que aparentemente estão associadas a mudanças na disponibilidade de diferentes itens alimentares. Os presentes resultados mostraram claramente que P. aethiopicus é um mamífero onívoro, capaz de se adaptar a uma grande variedade de composições dietéticas nos locais de estudo.
Assuntos
Animais , Ecossistema , Ouriços , Arábia Saudita , Ecologia , Comportamento AlimentarRESUMO
A significant level of yield losses in the vegetable and fruit farms are attributed to the weed populations of the area. This study was conducted for eighteen months during 2019-2020 to assess the presence of various weed families, related species, and their densities in the vegetable (CROP A) and fruit (CROP B) farms of Taif, Saudi Arabia. During the study, fourteen and ten weed families were noted in the vegetable and fruit farms of the studied area, respectively. Poaceae family weeds (729) were significantly higher in numbers followed by the families Asteraceae (414), Chenopodiaceae (338), and Gisekiaceae (153). In the vegetable farms, two weed species of the family Poaceae including Setaria viridis (437) and Eleusine indica ssp (277) were present in the higher numbers followed by Chenopodium murale (166) of the Chenopodiaceae family. In the fruit farms, the weed species Cynodon dactylon of the family Poaceae represented the highest weed density (172) followed by Gisekia pharnaceoides L. species (153) of family Gisekiaceae and Portulaca oleracea (59) belonging to the family Portulacaceae. The vegetable and fruit farms of the Taif region face severe weed pressure that affects the cultivated crops. This study depicts a comprehensive picture of weed diversity and density in the vegetable and fruit farms of the area. The results of this study will be beneficial in developing effective weed management practices for better crop production.
Um nível significativo de perdas de rendimento nas fazendas de hortaliças e frutas é atribuído às populações de ervas daninhas da área. Este estudo foi conduzido por dezoito meses durante 2019-2020 para avaliar a presença de várias famílias de ervas daninhas, espécies relacionadas e suas densidades nas fazendas de vegetais (CROP A) e frutas (CROP B) de Taif, Arábia Saudita. Durante o estudo, catorze e dez famílias de plantas daninhas foram observadas nas fazendas de hortaliças e frutas da área estudada, respectivamente. As plantas daninhas da família Poaceae (729) foram significativamente maiores em número, seguidas pelas famílias Asteraceae (414), Chenopodiaceae (338) e Gisekiaceae (153). Nas hortas, duas espécies de plantas daninhas da família Poaceae, incluindo Setaria viridis (437) e Eleusine indica ssp (277), estavam presentes em maior número, seguidas por Chenopodium murale (166) da família Chenopodiaceae. Nas fruticulturas, a espécie de plantas daninhas Cynodon dactylon da família Poaceae representou a maior densidade de plantas daninhas (172) seguida das espécies Gisekia pharnaceoides L. (153) da família Gisekiaceae e Portulaca oleracea (59) pertencentes à família Portulacaceae. As fazendas de hortaliças e frutas da região de Taif enfrentam uma forte pressão de ervas daninhas que afeta as culturas cultivadas. Este estudo apresenta um quadro abrangente da diversidade e densidade de plantas daninhas nas fazendas de hortaliças e frutas da região. Os resultados deste estudo serão benéficos no desenvolvimento de práticas eficazes de manejo de plantas daninhas para uma melhor produção das culturas.
Assuntos
Verduras/crescimento & desenvolvimento , Plantas Daninhas/crescimento & desenvolvimento , Fazendas , Frutas/crescimento & desenvolvimento , Arábia SauditaRESUMO
The Positive Mental Health (PMH) scale has been shown to be a reliable and valid tool for assessing positive mental health and well-being in different languages and cultures. However, the PMH scale has not yet been translated into Arabic and validated for the Saudi Arabian population. Therefore, the current study aimed to translate the English version of the PMH scale into Arabic for the Saudi Arabian context and validate the translated scale. A total of 1148 adult participants from Saudi public universities took part in the study. Based on exploratory and confirmatory factor analyses in different subsamples, the results of the current study revealed that the unifactorial model satisfactorily fits the data. Additionally, the Arabic version of the PMH scale demonstrated sufficient levels of reliability and had a high negative correlation with the Beck Depression Inventory-II, indicating convergent validity. Taken together, the findings of the current study suggest that the Arabic version of the PMH scale has appropriate levels of validity and reliability for the Saudi Arabian population.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Saúde Mental , Inquéritos e Questionários , Reprodutibilidade dos Testes , Arábia Saudita , Tradução , Comparação Transcultural , Análise Fatorial , Psicologia PositivaRESUMO
ABSTRACT@#Smile aesthetic, known as the static and dynamic relationship of the dentition and supporting structures to the facial soft tissues, is one of the most important elements of facial attractiveness. The objective of the study was to assess the perception of smile aesthetics and attractiveness through digital image manipulation of aesthetic variables and to compare those perceptions according to diverse sociodemographic data among female Saudi laypeople attending the dental clinic. A crosssectional study of 193 female Saudi participants were randomly selected and consented to answer the study questionnaire. Nine smile photograph images were created to compare different smile aesthetic perceptions. Two groups were recruited: 120 participants in the first group (under 30 years old) and 73 participants in the second group (30 years old or above). All participants in both groups were asked to choose the attractiveness of each smile image using multiple-choice options. A statistically significant finding showed that normal buccal corridors were chosen as the most attractive smile by 42.5% of the participants in the younger group and by a significantly higher ratio of the participants with a bachelor’s degree or higher level of education at 49% (p < 0.05). Laypeople’s preferences regarding smile attractiveness vary, but a normal appearance was the ideal choice for the majority. Orthodontic treatment should consider the general sociocultural understanding of smile perception.