Importancia del ejercicio físico en las personas con diabetes mellitus / Importance of physical exercise in people with diabetes mellitus

Introducción: el ejercicio físico constituye uno de los pilares fundamentales en el tratamiento de las personas con diabetes. Ajustar el régimen terapéutico permite una participación segura y un alto desempeño de la actividad física. Objetivo: describir los efectos y la importancia del ejercicio físico en las personas con diabetes mellitus. Método: se realizó una investigación documental, se utilizó como buscador de información científica Google Académico. Se evaluaron libros, artículos de investigación y de revisión de diferentes bases de datos: LILACS, PubMed, SciElo, Cochrane y páginas web, en idioma español, inglés o portugués. Resultados: en las personas con diabetes se recomiendan los ejercicios físicos aeróbicos, los mismos incrementan la sensibilidad a la insulina influyendo favorablemente sobre el control metabólico; los ejercicios de resistencia también son beneficiosos con el objetivo de mejorar la fuerza muscular. Antes de desarrollar un programa de ejercicios, la persona con diabetes mellitus debe someterse a una evaluación médica detallada. La indicación del tipo de ejercicio, su intensidad y duración debe ser personalizada. El control glucémico antes, durante y después del ejercicio es fundamental. En los niños pequeños fomentar el juego es la mejor manera de garantizar una actividad física placentera. Conclusiones: el ejercicio físico debe indicarse en las personas con diabetes mellitus por sus múltiples beneficios relacionados con la salud. Su indicación debe ser individualizada

Introduction: Physical exercise is one of de fundamental pillars in the treatment of people with diabetes. Adjusting the therapeutic regimen allows safe participation and high performance of physical activity. Aim: To describe the effects and importance of physical exercise in people with diabetes mellitus. Method: A documentary investigation was carried out. It was used as a search engine for scientific information Google Academic. Books, research and review articles from different databases were evaluated: LILACS, PubMed, SciElo, Cochrane and web pages in Spanish, English and Portuguese. Results: In people with diabetes, aerobic physical exercises are recommended, they increase insulin sensitivity and have a favorable influence on metabolic control. Resistance exercises are also beneficial in order to improve muscle strength. Before developing an exercise program, the person with diabetes mellitus must undergo a detailed medical evaluation. The indication of the type of exercise, its intensity and duration must be personalized. Glycemic control before, during and after exercise is essential. Encouraging play in young children is the best way to ensure enjoyable physical activity. Conclusions: The physical exercise should be indicated in people with diabetes mellitus due to its multiple health- related benefits; its indication must be individualized.

Wogonoside alleviates high glucose-induced dysfunction of retinal microvascular endothelial cells and diabetic retinopathy in rats by up-regulating SIRT1 / 南方医科大学学报

OBJECTIVE@#To investigate the effects of wogonoside on high glucose-induced dysfunction of human retinal microvascular endothelial cells (hRMECs) and streptozotocin (STZ)-induced diabetic retinopathy in rats and explore the underlying molecular mechanism.@*METHODS@#HRMECs in routine culture were treated with 25 mmol/L mannitol or exposed to high glucose (30 mmol/L glucose) and treatment with 10, 20, 30, 40 μmol/L wogonoside. CCK-8 assay and Transwell assay were used to examine cell proliferation and migration, and the changes in tube formation and monolayer cell membrane permeability were tested. ROS, NO and GSH-ST kits were used to evaluate oxidative stress levels in the cells. The expressions of IL-1β and IL-6 in the cells were examined with qRT-PCR and ELISA, and the protein expressions of VEGF, HIF-1α and SIRT1 were detected using Western blotting. We also tested the effect of wogonoside on retinal injury and expressions of HIF-1α, ROS, VEGF, TNF-α, IL-1β, IL-6 and SIRT1 proteins in rat models of STZ-induced diabetic retinopathy.@*RESULTS@#High glucose exposure caused abnormal proliferation and migration, promoted angiogenesis, increased membrane permeability (P < 0.05), and induced inflammation and oxidative stress in hRMECs (P < 0.05). Wogonoside treatment concentration-dependently inhibited high glucose-induced changes in hRMECs. High glucose exposure significantly lowered the expression of SIRT1 in hRMECs, which was partially reversed by wogonoside (30 μmol/L) treatment; interference of SIRT1 obviously attenuated the inhibitory effects of wogonoside against high glucose-induced changes in proliferation, migration, angiogenesis, membrane permeability, inflammation and oxidative stress in hRMECs. In rat models of STZ-induced diabetic retinopathy, wogonoside effectively suppressed retinal thickening (P < 0.05), alleviated STZ-induced retinal injury, and increased the expression of SIRT1 in the retinal tissues (P < 0.001).@*CONCLUSION@#Wogonoside alleviates retinal damage caused by diabetic retinopathy by up-regulating SIRT1 expression.

tiRNA-Val promotes angiogenesis via Sirt1-Hif-1α axis in mice with diabetic retinopathy

BACKGROUND: Diabetic retinopathy (DR) is a specific microvascular complication arising from diabetes, and its pathogenesis is not completely understood. tRNA-derived stress-induced RNAs (tiRNAs), a new type of small noncoding RNA generated by specific cleavage of tRNAs, has become a promising target for several diseases. However, the regulatory function of tiRNAs in DR and its detailed mechanism remain unknown. RESULTS: Here, we analyzed the tiRNA profiles of normal and DR retinal tissues. The expression level of tiRNA-Val was significantly upregulated in DR retinal tissues. Consistently, tiRNA-Val was upregulated in human retinal microvascular endothelial cells (HRMECs) under high glucose conditions. The overexpression of tiRNA-Val enhanced cell proliferation and inhibited cell apoptosis in HRMECs, but the knockdown of tiRNA-Val decreased cell proliferation and promoted cell apoptosis. Mechanistically, tiRNA-Val, derived from mature tRNA-Val with Ang cleavage, decreased Sirt1 expression level by interacting with sirt1 3'UTR, leading to the accumulation of Hif-1α, a key target for DR. In addition, subretinal injection of adeno-associated virus to knock down tiRNA-Val in DR mice ameliorated the symptoms of DR. CONCLUSION: tiRNA-Val enhance cell proliferation and inhibited cell apoptosis via Sirt1/Hif-1α pathway in HRMECs of DR retinal tissues.

Prevalencia de alteraciones del metabolismo glucídico en familiares de primer grado de pacientes diabéticos tipo 2 / Prevalence of glucidic metabolism alterations in first- degree relatives of type 2 diabetic patients

Introducción: La diabetes mellitus se considera un síndrome heterogéneo con etiología compleja en el que influyen factores genéticos y ambientales. Objetivo: Identificar la presencia de alteraciones del metabolismo glucídico y factores de riesgo aterogénicos en familiares de primera línea de pacientes diabéticos tipo 2. Métodos: Se realizó un estudio descriptivo de corte transversal que incluyó a 120 pacientes adultos, hijos de pacientes diabéticos, en los que no se encontraban antecedentes de alteraciones del metabolismo glucídico, pertenecientes al Policlínico Universitario Héroes del Moncada, del municipio Plaza de la Revolución. Se estudiaron variables sociodemográficas, variables clínicas y relacionadas con los estilos de vida como la tensión arterial, el índice de masa corporal, colesterol, triglicéridos, glucemias (ayunas y posprandial), hábito de fumar, actividad física y hábitos dietéticos. Resultados: Los pacientes tenían una edad promedio de 54,42 años y predominó el sexo femenino. Se detectaron alteraciones del metabolismo glucídico en 28,3 por ciento de los cuales 23,3 por ciento se consideraron prediabéticos y 5 por ciento diabéticos. Los factores de riesgo que predominaron fueron la dieta inadecuada, obesidad abdominal, hipercolesterolemia e hipertrigliceridemia que fueron más evidentes en los pacientes diagnosticados como diabéticos. Conclusiones: Los familiares de primer grado de pacientes diabéticos pueden presentar una alta prevalencia de alteraciones del metabolismo glucídico y factores de riesgo aterogénicos, aún sin sintomatología evidente, lo que refuerza la necesidad de realizar un diagnóstico temprano para evitar la progresión de la enfermedad(AU)

Introduction: Diabetes mellitus is considered a heterogeneous syndrome with a complex etiology, influenced by genetic and environmental factors. Objective: To identify the presence of alterations of the glucidic metabolism and atherogenic risk factors in first- degree relatives of type 2 diabetic patients. Methods: A descriptive cross-sectional study was carried out at Heroes del Moncada University Polyclinic, in Plaza de la Revolution municipality. The study included 120 adult patients, descendants of diabetic patients. They had no history of alterations of the glucidic metabolism. Sociodemographic, clinical variables were studied, and those related to lifestyles such as blood pressure, body mass index, cholesterol, triglycerides, (fasting and postprandial) glycaemia, smoking, physical activity and dietary habits. Results: These patients had average age of 54.42 years and the female sex predominated. Alterations of the glucidic metabolism were detected in 28.3 percent, 23.3 percent of them were considered pre-diabetic and 5 percent diabetic. The predominant risk factors were inadequate diet, abdominal obesity, hypercholesterolemia, and hypertriglyceridemia, which was much evident in patients diagnosed as diabetic. Conclusions: The first-degree relatives of diabetic patients may present high prevalence of alterations of glucidic metabolism and atherogenic risk factors, even with no evident symptoms, which reinforces the need of early diagnosis to avoid the progression of the disease(AU)

Asociación entre programa de intervención educativa integral y control metabólico de usuarios diabéticos tipo 2 insulino requirentes de Centro de Salud Familiar Cardenal Raúl Silva Henríquez, período 2015-2016 Chile / The effect of an educational intervention on the metabolic control of diabetic patients

Background: Diabetic patients have a poor adherence to lifestyle changes and a low compliance with medications. Aim: To study the effect of an educational intervention on the metabolic control of patients with diabetes mellitus. Patients and Methods: We studied insulin requiring diabetic patients attended at a primary health care clinic, who were randomly divided in two groups. One group participated in four modules of an educative intervention, carried out in four occasions. The other group did not participate in the educational sessions. Clinical, anthropometric and laboratory variables of the participants were obtained from their medical records, at three and six months after the intervention. Results: We recruited 22 men aged 64 ± 14 years and 48 women aged 63 ± 10 years. Thirty-six of these received the educational intervention. Eighty four percent were overweight or obese. The proportion of compensated patients, defined as those having a glycosylated hemoglobin between 7 and 9%, increased significantly after the educational intervention (χ2 = 7.9 p < 0.01, odds ratio: 0.2). Conclusions: A educational intervention in patients with insulin requiring diabetes mellitus improved the metabolic control of their disease.

TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes

SUMMARY OBJECTIVE Diabetes is a risk factor for acute kidney injury (AKI). However, its mechanism of pathogenesis has not been elucidated. The aim of the study was to investigate the role of inflammation and the toll-like receptor 7 (TLR7) in ischemic AKI for diabetes. METHODS A high glucose hypoxia-reoxygenation model of human renal tubular epithelial (HK-2) cells was used to generate AKI induced by ischemia-reperfusion in diabetes. The activity of cells was measured by CCK-8 assay and LDH activity. Inflammatory cytokines were assessed by ELISA. TLR7, MyD88, and NF-κB expressions were examined by western blotting. Apoptosis was evaluated by flow cytometry. RESULTS The high glucose group and low glucose group were subjected to hypoxia-reoxygenation. The low glucose group developed only mild cell damage, apoptosis, and inflammatory response. In contrast, an equivalent hypoxia-reoxygenation injury provoked severe cell damage, apoptosis, and inflammatory response in the high glucose group. Expression of TLR7 and its related proteins were measured in the high glucose group before and after hypoxia-reoxygenation. The high glucose group exhibited more significant increases in TLR7 expression following hypoxia-reoxygenation than the low glucose group. In addition, the expression of TLR7 and its related proteins after hypoxia-reoxygenation were higher in the high glucose group than in the low glucose group. Inhibition of TLR7 provides significant protection against ischemic injury in diabetes. CONCLUSION Our results suggest that diabetes increases the vulnerability to ischemia-induced renal injury. This increased vulnerability originates from a heightened inflammatory response involving the TLR7 signal transduction pathway.

RESUMO OBJETIVO O diabetes é um fator de risco para a lesão renal aguda (LRA). No entanto, seu mecanismo de patogênese não foi elucidado. O objetivo do estudo foi investigar o papel da inflamação e do receptor Toll-like 7 (TLR7) na LRA isquêmica no diabetes. MÉTODOS Um modelo de hipóxia-reoxigenação de células epiteliais tubulares renais humanas (HK-2) na presença de concentrações altas de glicose foi utilizado para gerar LRA induzida por isquemia-reperfusão em diabetes. A atividade das células foi medida pelo ensaio Cell Counting Kit-8 (CCK-8) e pela atividade da lactato desidrogenase (LDH). As citocinas inflamatórias foram avaliadas por ensaio imunoenzimático (Elisa). A expressão de TLR7, do fator de diferenciação mieloide 88 (MyD88) e do fator de transcrição nuclear-κB (NF-κB) foi examinada por Western blotting. A apoptose foi avaliada por citometria de fluxo. RESULTADOS Os grupos glicose alta e glicose baixa foram submetidos à hipóxia-reoxigenação. O grupo de baixa glicose desenvolveu apenas danos celulares ligeiros, apoptose e uma resposta inflamatória. Em contraste, no grupo de alta glicose, uma lesão equivalente de hipóxia-reoxigenação provocou danos celulares graves, apoptose e uma resposta inflamatória. A expressão de TLR7 e suas proteínas relacionadas foi medida no grupo de alta glicose antes e após a hipóxia-reoxigenação. O grupo de alta glicose exibiu maiores aumentos na expressão de TLR7 após hipóxia-reoxigenação do que o grupo de baixa glicose. Além disso, a expressão de TLR7 e suas proteínas relacionadas após a hipóxia-reoxigenação foi maior no grupo com alto nível de glicose do que no grupo com baixo nível de glicose. A inibição do TLR7 fornece proteção significativa contra a lesão isquêmica no diabetes. CONCLUSÃO Nossos resultados sugerem que o diabetes aumenta a vulnerabilidade à lesão renal induzida por isquemia. Essa vulnerabilidade acrescida tem por origem uma resposta inflamatória aumentada envolvendo a via de transdução de sinal do TLR7.

Mecanismos involucrados en la fragilidad ósea en diabetes mellitus / Mechanisms involved in the bone fragility in diabetes mellitus

La diabetes es una enfermedad crónica asociada con importantes comorbilidades. El sistema esquelético parece ser un objetivo adicional de daño mediado por diabetes. Se acepta que la diabetes tipo 1 y tipo 2 se asocian con un mayor riesgo de fractura ósea. Varios estudios han demostrado que los cambios metabólicos causados por la diabetes pueden influir en el metabolismo óseo disminuyendo la calidad y la resistencia del hueso. Sin embargo, los mecanismos subyacentes no se conocen por completo pero son multifactoriales y, probablemente, incluyen los efectos de la obesidad, hiperglucemia, estrés oxidativo y acumulación de productos finales de glicosilación avanzada. Estos darían lugar a un desequilibrio de varios procesos y sistemas: formación de hueso, resorción ósea, formación y entrecruzamiento de colágeno. Otros factores adicionales como la hipoglucemia inducida por el tratamiento, ciertos medicamentos antidiabéticos con un efecto directo sobre el metabolismo óseo y mineral, así como una mayor propensión a las caídas, contribuirían al aumento del riesgo de fracturas en pacientes con diabetes mellitus. Esta revisión tiene como objetivo describir los mecanismos fisiopatológicas subyacentes a la fragilidad ósea en pacientes diabéticos. (AU)

Diabetes is a chronic disease associated with important comorbidities. The skeletal system seems to be an additional target of diabetes mediated damage. It is accepted that type 1 and type 2 diabetes are associated with an increased risk of bone fracture. Several studies have shown that metabolic changes caused by diabetes can influence bone metabolism by decreasing bone quality and resistance. However, the underlying mechanisms are not completely known but they are multifactorial and probably include the effects of obesity, hyperglycemia, oxidative stress and accumulation of advanced glycosylation end products. These would lead to an imbalance of several processes and systems: bone formation, bone resorption, formation and collagen crosslinking. Other additional factors such as treatment-induced hypoglycemia, certain antidiabetic medications with a direct effect on bone and mineral metabolism, as well as an increased propensity for falls, would contribute to the increased risk of fractures in patients with diabetes mellitus. This review aims to describe the pathophysiological mechanisms underlying bone fragility in diabetic patients. (AU)

Effects of diabetes and hypertension on oral mucosa and tgfß1 salivary levels

Abstract The aim of this study was to investigate salivary levels of TGFβ1 and proliferation/ maturation of epithelial mucosa cells in diabetic and hypertensive patients. Design: in this cross-sectional study, whole stimulated saliva and oral mucosa exfoliative cytology specimens were collected from 39 patients that were healthy (control, n=10) or presented history of arterial hypertension (HAS, n=9), diabetes mellitus (DM, n=10) or both (DM+HAS, n=10). Salivary flow rate (SFR), TGFβ1 level in saliva, AgNORs and the epithelial maturation were evaluated. Non-parametric Kruskal-Wallis test, followed by Dunn's multiple comparison post-test and the Spearman test correlation analysis were used. SFR showed a significant decreased in DM and DM+HAS (0.47±0.11 and 0.64±0.43 mL/min) when compared to control (1.4±0.38 mL/min). DM+HAS presented the highest value of TGFβ1 concentration (24.72±5.89 pg/mL). It was observed a positive correlation between TGFβ1 and glycaemia (R=0.6371; p<0.001) and a negative correlation between TGFβ1 and saliva (R=-0.6162; p<0.001) and glycaemia and SFR (R=-0.5654; P=0.001). AgNORs number and status of maturation of mucosa cells were similar for all conditions. DM and DM+HAS presented the lowest SFR, which correlated with increased TGFβ1 levels. Despite the higher TGFβ1 secretion it was not observed changes in the morphology or proliferation of epithelial cells when diabetes or hypertension was present.

Resumo O objetivo deste estudo foi investigar os níveis de TGFβ1 na saliva e a proliferação/maturação das células epiteliais da mucosa em paciente diabéticos e hipertensos. Neste estudo transversal, saliva estimulada e amostras de citologia exfoliativa de mucosa oral foram coletadas de um total de 39 pacientes que se apresentavam saudáveis (controle, n=10) ou com história de hipertensão arterial (HAS, n=9), diabetes mellitus (DM, n=10) ou ambos (DM+HAS, n=10). Taxa de fluxo salivar (SFR), níveis de TGFβ1 na saliva, AgNORs e maturação epitelial foram avaliados. Teste não-paramétrico de Kruskal-Wallis, seguido de comparação múltipla de Dunn e correlação de Spearman foram utilizados para as análises. SFR diminuiu significantemente em DM e DM+HAS (0,47±0,11 e 0,64±0,43 mL/min) quando comparado ao controle (1,4±0,38 mL/min). DM+HAS apresentou os maiores valores de concentração de TGFβ1 (24,72±5,89 pg/mL). Foi observada uma correlação positiva entre TGFβ1 e glicemia (R=0,6371; p<0,001) e uma correlação negativa entre TGFβ1 e saliva (R=-0,6162; p<0,001) e glicemia e SFR (R=-0,5654; p=0,001). Número de AgNORs e o padrão da maturação das células epiteliais foram similares entre os todos grupos. DM e DM+HAS apresentaram os menores valores de SFR, os quais foram correlacionados com o aumento nos níveis de TGFβ1. Apesar da maior secreção de TGFβ1, não foram observadas mudanças na morfologia ou proliferação das células epiteliais quando o paciente apresentava diabetes ou hipertensão.

Efectividad del tratamiento periodontal en el control metabólico de pacientes con diabetes mellitus / Effectiveness of the periodontal treatment in the metabolic control of patients with diabetes mellitus

Se efectuó un estudio de intervención terapéutica en 60 pacientes con diabetes mellitus y periodontitis leve, atendidos en la consulta estomatológica del Hospital General Docente Dr Juan Bruno Zayas Alfonso de Santiago de Cuba, con vistas a determinar si el tratamiento realizado favorecería el control metabólico de los afectados, desde noviembre de 2015 hasta igual mes de 2016. Se formaron 2 grupos de manera aleatoria con 30 integrantes cada uno: de estudio (números pares), quienes recibieron la fase inicial del tratamiento, raspado y alisado radicular; de control (números impares), se les indicó fase inicial e irrigaciones subgingivales con clorhexidina. Antes y después del tratamiento periodontal se realizaron exámenes de glucemia y hemoglobina glicosilada. Se utilizó la prueba de Ji al cuadrado de homogeneidad para la comparación entre dichos grupos, con una significación de 95%. Se concluyó que la terapia periodontal fue eficaz en el control metabólico de los pacientes

A study of therapeutic intervention in 60 patients with diabetes mellitus and light periodontitis, assisted in the estomatological service of Dr Juan Bruno Zayas Alfonso Teaching General Hospital in Santiago de Cuba, aimed at determining if the treatment would favor the metabolic control of the affected patients, was carried out from November, 2015 to the same month in 2016. Two groups were formed at random with 30 members each: a study group (even numbers) who received the initial phase of the treatment, radicular scrapring and smoothing; a control group (odd numbers), they were indicated initial phase and subgingival irrigations with chlorhexidine. Before and after the periodontal treatment glycemia and glycosylated hemoglobin exams were carried out. The chi-square test of homogeneity was used for the comparison among these groups, with a significance of 95%. It was concluded that the periodontal therapy was effective in the metabolic control of the patients

Prevalencia del Síndrome Metabólico en pacientes internadas en el Servicio de Ginecología del Hospital Central. Instituto de Previsión Social, Enero-Junio 2017 / Prevalence of the Metabolic Syndrome in patients admitted to the Gynecology Service of the Central Hospital. Institute of Social Security, January-June 2017

Introducción: El Síndrome Metabólico (SM) es una epidemia y un problema de salud pública, debido a la creciente prevalencia de obesidad y estilos de vida poco saludables. Está asociado a un incremento de 5 veces de riesgo de Diabetes Mellitus tipo 2, y de 2 a 3 veces de aumento en el riesgo de enfermedad cardiovascular, con disminución en la supervivencia. Después de la menopausia, la prevalencia de SM aumenta todavía más, generando un aumento muy significativo del riesgo cardiovascular. Objetivos: Conocer la prevalencia de SM en pacientes internadas de enero a junio del 2017 en el Servicio de Ginecología HC-IPS y comparar la prevalencia de SM obtenida según criterios de la NCEP ATPIII y la IDF en mujeres pre menopáusicas y post menopáusicas. Metodología: Estudio retrospectivo, descriptivo de corte transversal. Resultados: De 380 pacientes observadas, el promedio de edades fue de 43 años.77 % tenían un IMC mayor a 25. 56,8 % eran pre menopáusicas y 43,1 % post menopáusicas. La prevalencia de SM fue diferente según criterios de NCEP ATPIII y la IDF, siendo 23,1 % con el primero y 63 % con el segundo. Se encontró que 80% de las pre menopáusicas y 90 % de las post menopáusicas presentaban IMC mayor a 25. La patología ginecológica mayormente asociada fue el engrosamiento endometrial, observado en un 18% de los casos de SM en las post menopáusicas. En las pre menopáusicas se observó que en el 40% el SM estaba relacionado a HUA y miomatosis uterina. El porcentaje de cáncer endometrial fue bastante importante siendo del 11%. Palabras claves: menopausia, hipertensión, diabetes.

Introduction: The Metabolic Syndrome (MS) is an epidemic and a public health problem, due to the growing prevalence of obesity and unhealthy lifestyles. It is associated with a 5-fold increase in the risk of Diabetes Mellitus type 2, and a 2 to 3-fold increase in the risk of cardiovascular disease, with a decrease in survival. After menopause, the prevalence of MS increases even more, generating a very significant increase in cardiovascular risk. Objectives: To know the prevalence of MS in patients hospitalized from January to June 2017 in the HC-IPS Gynecology Service and to compare the prevalence of MS obtained according to NCEP ATPIII and IDF criteria in premenopausal and postmenopausal women. Methodology: Retrospective, descriptive crosssectional study. Results: Of 380 patients observed, the average age was 43.77% had a BMI greater than 25. 56.8% were premenopausal and 43.1% postmenopausal. The prevalence of MS was different according to the criteria of NCEP ATPIII and the IDF, being 23.1% with the first and 63% with the second. It was found that 80% of premenopausal and 90% of postmenopausal women had a BMI greater than 25. The gynecological pathology most associated was endometrial thickening, observed in 18% of cases of MS in postmenopausal women. In premenopausal women it was observed that in 40% the MS was related to HUA and uterine myomatosis. The percentage of endometrial cancer was quite important being 11%. Keywords: menopause, hypertension, diabetes

C-Peptides for diagnostics and therapy: a veterinary medicine point of view / Peptídeos-C para diagnóstico e terapia: um ponto de vista na medicina veterinária

Empirical studies proved that C-peptides are performing numerous intrinsic biological roles, and serve as a marker for pancreatic performance analysis. Since the last decade, C-peptide assays for differential diagnosis in veterinary diabetic patients are becoming more available, but still only for a very limited number of species. Studies on C-peptide as a diagnostic tool, therapy for associated complications, or as replacement therapies for C-peptide deficiency still showed not to be a common practice in veterinary medicine. This review was conducted to determine the potential importance of C-peptide in Veterinary Medicine, relevant in the diagnosis of diabetes and for other metabolic processes, as well as its proposed therapeutic benefits. Numerous articles were identified that reported positive results in their experimental studies, whether C-peptide as a biomarker for pancreatic performance in dogs, cats, and horses, as a non-invasive method to monitor nutritional status in primates, or to investigate its potential therapeutic benefits for diabetes-related illnesses.(AU)

Os estudos empíricos provaram que o peptídeo C realiza várias funções biológicas intrínsecas e serve também como um marcador para a análise de desempenho do pâncreas. Nesta última década os ensaios peptídeo C para o diagnóstico diferencial em doentes diabéticos veterinários estão mais disponíveis, contudo apenas em um limitado número de espécies. Estudos sobre C-peptídeo como ferramenta de diagnóstico, terapia de complicações associadas ou como terapias de reposição na sua deficiência ainda não é prática comum na Medicina Veterinária. Esta avaliação foi realizada para determinar a importância potencial de peptídeo C na Medicina Veterinária, relevante no diagnóstico de diabetes e também em outros processos metabólicos, assim como os benefícios terapêuticos propostos. Foram identificados numerosos artigos que reportaram resultados positivos nos seus estudos experimentais, quer o peptídeo C fosse utilizado como biomarcador para o desempenho do pâncreas em cães, gatos e cavalos, como um método não invasivo para monitorar o estado nutricional em primatas, quer para investigar o seu potencial terapêutico benéfico para doenças relacionadas ao diabetes.(AU)

Fatores Lipídicos de Risco Cardiovascular e Taxa de Eventos Cardiovasculares após Revascularização Miocárdica / Cardiovascular Lipid Risk Factors and Rate of Cardiovascular Events After Myocardial Revascularization

Fundamentos: A doença arterial coronariana é um problema de saúde pública, e principal causa de morte prematura na Europa e no mundo. Há evidência epidemiológica de que o controle dos fatores de risco cardiovasculares após a revascularização do miocárdio encontra-se ainda abaixo do desejável.Objetivo: Analisar a incidência de eventos cardiovasculares em pacientes em tratamento para prevenção secundária após revascularização do miocárdio. Método: Estudo unicêntrico com pacientes com doença coronariana consecutivos, identificados retrospectivamente. O estudo foi conduzido no Instituto de Doenças Cardiovasculares em Timisoara, Romênia. Os pacientes com diagnóstico de revascularização por doença arterial coronariana (intervenção coronária percutânea, ICP ou ponte de artéria coronária, CABG) foram incluídos, seguindo-se os critérios de inclusão do estudo EUROASPIRE IV. Os dados foram analisados em três momentos ­ T0, quando a revascularização do miocárdio foi realizada; T1 (entrevista com pacientes submetidos à revascularização há mais de 6 meses e menos de 5 anos); e T2 (entrevista com pacientes submetidos à revascularização há mais de 5 anos). Resultados: Dos 375 pacientes com doença coronariana, 341 foram incluídos no estudo. No T1, somente 5% e 34,9% dos pacientes atingiram os níveis desejáveis de LDL-c e não-HDL-c, respectivamente. A taxa de MACE em T2 foi de 7,9% em um tempo de acompanhamento mediano de 4,33 anos. Encontramos uma associação significativa positiva entre taxa de MACE e LDL-c em T1 (p = 0,039). Houve diferenças significativas nos níveis médios de não-HDL-c entre as categorias de MACE em T1 (p = 0,02). Valores médios de não-HDL associaram-se significativamente com a incidência de insuficiência cardíaca (p = 0,007), diabetes com diagnóstico recente (p = 0,017) e taxa de reestenose (p = 0,04). Conclusão: O estudo destaca a necessidade de se controlar fatores de risco relacionados ao perfil lipídico após procedimentos de revascularização do miocárdio, mesmo em longo prazo, a fim de minimizar o risco de eventos cardiovasculares em pacientes com doenças coronarianas

Background: Coronary heart disease is a public health problem, and the leading cause of premature death in Europe and worldwide. There is epidemiological evidence that the control of cardiovascular risk factors following myocardial revascularization remains suboptimal. Objectives: Analyze the incidence of cardiovascular events in patients receiving secondary prevention therapies after myocardial revascularization. Methods: Single-center study on consecutive coronary patients retrospectively identified, conducted at the Institute of Cardiovascular Diseases in Timisoara, Romania. Patients with diagnosis of revascularization for coronary artery disease (percutaneous coronary intervention, PCI or coronary artery bypass grafting, CABG) were included, following the inclusion criteria of the EuroAspire IV study. Outcome measures were assessed at three time points­ at T0, when myocardial revascularization was performed; at T1 (interview with patients who had undergone revascularization for more than 6 months and less than 5 years), and T2 (interview with patients who had undergone revascularization for more than 5 years). Associations of primary and secondary lipid targets with the rates of adverse cardiovascular events (MACE) were assessed at T2. Results: Of 375 coronary patients, 341 were included in the study. At T1, 5% and 34.9% of patients reached the LDL-c and non-HDL-c target respectively. MACE rate at T2 was 7.9% in a median of 4.33 years of follow-up. We found a positive, statically significant association between MACE rate and LDL-c at T1 (p = 0.039). There were significant differences in mean non-HDLc levels between MACE categories at T1 (p = 0.02). There was a significant association between mean non-HDL with the incidence of heart failure (p = 0.007), newly diagnosed diabetes (p = 0.017) and restenosis rate (p = 0.004). Conclusion: The study highlights the need to control lipid risk factors after myocardial revascularization procedures, even at long-term, to minimize the risk of cardiovascular events in patients with coronary diseases

Does diabetes alter immunolocalization of galectin-1 and galectin-3 in the rat ovary? / ¿La diabetes altera la inmunolocalización de galectina-1 y galectina-3 en el ovario de rata?

Diabetes mellitus (DM), is a metabolic disease occurring via insulin secretion deficiency from the pancreas and/or an insufficiency of tissue response to insulin. The present study is intended to show of immunolocalizations of beta-galactose-binding proteins Galectin-1 and Galectin-3 in diabetic rat ovarium and their relationship with diabetes. In this study, 8 to 10-week-old, 250­300 g weighing 50 mature female rats were used, in order to establish diabetes mellitus in those animals, 60 mg/kg intravenous streptozotocin was injected to each animal. After death, diabetics and non-diabetics rats's routine tissue processing steps is done to rat ovarial tissues for immunohistochemical investigation. Strong expressions of Galectin-1 and Galectin-3 were observed in the ovarial germinal epithelium and vascular endothelial. While the strong intense expression of Galectin-1 was seen in the zona pellucida, Galectin-3 expression was strongest in the cytoplesmic regions of cells. Zona pellucida has 3 protein complexes (ZP1, ZP2 and ZP3) in rats and in humans and they have the capability of recognizing the carbonhydrate fields in tissues. The strong expression of galectins in those regions could be the result of carbonhydrate binding properties expression of Gal-3 in the cytoplasmic regions of growing follicles could suggest the idea that Gal-3 could have effects on follicle growth. In conclusion, beta galactose-binding proteins Gal-1 and Gal-3 had stronger immunolocalization in diabetic rat ovarium when compared to the controls. Diabetes could increase the Gal-1 and Gal-3 expressions in the ovarial tissue.

La diabetes mellitus (DM) es una enfermedad metabólica debido a una deficiencia en la secreción de insulina por parte del páncreas o por una insuficiente respuesta de los tejidos a la insulina. El objetivo fue demostrar la inmunolocalización de las proteínas de unión beta-galactosa Galectina-1 y Galectina-3 en los ovarios de ratas diabéticas y su relación con la diabetes. Fueron utilizadas 50 ratas hembras maduras entre 8­10 semanas de edad, con un peso de 250­300 g. Con el fin de desarrollar DM en los animales, se inyectó a cada uno 60 mg/kg de estreptozotocina vía intravenosa. Después de la eutanasia, se realizó el procesamiento de rutina de los tejidos de las ratas diabéticas y no diabéticas para evaluar los tejidos ováricosa través de inmunohistoquímica. Se observaron expresiones fuertes de la Galectina-1 y Galectina-3 en el epitelio germinal y epitelio endotelial vascular del ovario. Si bien la fuerte e intensa expresión de Galectina-1 se observó en la zona pelúcida, la Galectina-3 tuvo una expresión más fuerte en las regiones de las células citoplasmáticas. La zona pelúcida tiene 3 complejos de proteínas (ZP1, ZP2 y ZP3) en ratas y en seres humanos y tienen la capacidad de reconocer los campos de carbohidratos en los tejidos. La fuerte expresión de las galectinas de esas regiones podría ser el resultado de las propiedades de unión a carbonhidratos expresión de Gal-3 en las regiones citoplasmáticas de los folículos en crecimiento, pudiendo sugerir que Gal-3 podría tener efectos sobre el crecimiento del folículo. En conclusión, las proteínas de unión beta-galactosa Gal-1 y Gal-3 tienen una mayor inmunolocalización en los ovarios de ratas diabéticas, en comparación a los controles. La diabetes podría incrementar las expresiones de Gal-1 y Gal-3 en el tejido ovárico.

Avaliação dos efeitos da infusão das folhas de Syzygium cumini em um modelo experimental de resistência à insulina em Drosophila melanogaster / Evaluation of the effects of infusion of leaves of Syzygium cumini in an experimental model of insulin resistance in Drosophila melanogaster

Introdução: a utilização de plantas como recurso terapêutico é uma prática bastante antiga, e desde então tem sido alvo de estudos. Neste contexto existe o jambolão (Syzygiumcumini) que é uma planta pertencente à família Mirtaceae. as folhas possuem substâncias com ação antidiabética, exercendo função hipoglicemiante, mimetizando as ações da insulina, regulando os níveis glicêmicos. Objetivo: avaliara atividade antioxidante das folhas de Syzygiumcumini e os efeitos desta infusão em um modelo experimental de dieta enriquecida com altas concentrações de glicose em Drosophila melanogaster. Métodos: para verificar a atividade antioxidante do jambolão utilizou-se o método DPPH (2,2-difenil-1picrilhidrazila) segundo Brand­Willians et al. (1995). As Drosophilas foram separadas por sexo, pesadas e tratadas com infusão de folhas de jambolão com exceção do controle, por três dias e no quarto dia foram sacrificadas e pesadas para análises bioquímicas de GlicosePAP Liquiform triglicerídeos enzimático (Labtest). Resultado e Discussão: em relação aos machos, o efeito do jambolão foi extremamente significativo como fator protetor na dieta com 20% de sacarose e também na dieta com 30% de sacarose. Conclusão: este estudo demonstrou que a exposição a uma dieta rica em carboidratos foi prejudicial à D. melanogaster e que o chá de Syzygiumcumini (jambolão) teve efeito positivo nos parâmetros de glicose e triglicérides, validando este modelo de invertebrado como ferramenta para a investigação da Diabetes Melitus.

Introduction: the use of plants as a therapeutic resource is a very ancient practice, and has since been the subject of studies. In this context there is jambolan (Syzygiumcumini) whichis a plant belonging to the family Mirtaceae. The leave shave substance swith anti diabetic action, acting hypoglycemic function, mimicking the actions of insulin, regulating blood glucose levels. Objective: the aim of this study was to evaluate the antioxidant activityof the leaves of Syzygium cumini and the effects of this infusion on a diet enriched experimental model with high glucose concentrations in Drosophila melanogaster. Methods: to verify jambolan the antioxidant used the DPPH (2,2-diphenyl-1picrilhidrazila) second Brand-Williams et al. (1995). The Drosophila were separated by sex, weighed and treated with infusion jambolan leaves except for the control, for three days and on the fourth day were sacrificed and weighed to biochemistry Glucose PAP Liquiform enzymatic triglycerides (Labtest). Results and discussion: regarding males, jambolan effect was highly significant as a protective factor in the diet with 20% sucroseandalso in the diet with 30% sucrose. Conclusion: this study demonstrated that exposure to a high-carbohydrate diet was harmful to D. melanogaster and the Syzygiumtea cumini (jambolan) had a positive effecton glucose and triglycerides parameters, validating this invertebrate model as a tool for the investigation of Diabetes mellitus.

Factores de riesgo asociados al mal control metabólico en pacientes con diabetes mellitus tratados con insulina / Risk factors associated with poor metabolic control in patients with diabetes mellitus treatedwith insulin

Introducción: la diabetes mellitus (DM) constituye un problema de salud pública prioritario, tanto por su incidencia, prevalencia y la morbimortalidad que de ella derivan. Objetivo: determinar los factores de riesgo asociados al mal control metabólico en pacientes con DM tratados con insulina e internados en la sala de Clínica Médica del Hospital Nacional. Metodología: diseño de casos (diabéticos con mal control metabólico al alta) y controles (diabéticos con buen control metabólico al alta). Muestreo no probabilístico de casos consecutivos. Resultados: ingresaron al trabajo 119 pacientes, con una edad media de 60 años, el 57% de ellos de sexo femenino y el 43% masculino. El tipo de DM predominante fue el tipo 2 (96,5 %). Todos se encontraban en tratamiento con insulina tanto rápida, intermedia y prolongada. El tipo de insulina no influyó sobre el control metabólico. La insulina NPH fue la de uso predominante. El grupo control utilizó menor dosis promedio de insulina. Se analizó los efectos de la hipertensión arterial, la enfermedad renal crónica e infecciones en el control glicémico y ninguna de ellas influyó sobre el control de la misma al alta. Conclusión: el único factor estadísticamente significativo para el buen control metabólico al alta fue el mayor tiempo de internación.

Introduction: diabetes mellitus (DM) is a priority public health problem, both for its incidence, prevalence, morbidity and mortality that derive from it. Objective: to determine the risk factors associated with poor metabolic control in patients with DM treated with insulin and admitted to the Internal Medicine Department of the Hospital Nacional. Metodology: cases design (diabetics with poor metabolic control at discharge) and control group. (Diabetics with metabolic control at discharge).Non-probabilistic sampling of consecutive cases.Results: 119 patients were recruited, with an average age of 60 years, 57% of them female and 43% male. The predominant type 2 DM was (96.5%). All were treated with both fast, intermediate and prolonged insulin. The type of insulin had no influence on metabolic control. NPH insulin was the predominant use. The control group used insulin doses lower than average. The effect of hypertension was analyzed, chronic kidney disease and infections in glycemic control and none of them influenced the control at discharge. Conclusion: the only statistically significant factor for good metabolic control at discharge was a longer hospital stay.

Estudo comparativo do índice tornozelo-braquial em diabéticos e não diabéticos com isquemia crítica / Comparative study of the ankle-brachial index in diabetic and non-diabetic patients with critical limb ischemia

Contexto A calcificação da camada média arterial pode tornar o Índice Tornozelo-Braquial (ITB) falsamente elevado em diabéticos, dificultando a avaliação da doença arterial. Objetivo Comparar os valores do ITB de diabéticos e não diabéticos com isquemia crítica. Métodos Foram incluídos 140 pacientes (60% de diabéticos) acompanhados no Serviço de Cirurgia Vascular do Complexo Hospitalar Universitário Professor Edgard Santos com isquemia crítica por DAOP infra-inguinal. Comparou-se a média dos valores do ITB dos dois grupos de pacientes, correlacionando o ITB com a gravidade da isquemia, segundo a Classificação de Rutherford. A análise estatística foi realizada pelo EPI-INFO. Resultados A maioria dos 140 pacientes (77%) se encontrava na Categoria 5 da Classificação de Rutherford, 6% na 4 e 17% na 6. Nove diabéticos (11%) e um não diabético (2%) apresentaram ITB > 1,15 (p = 0,02), sendo excluídos da análise das médias do ITB. Considerando os 130 pacientes, os 75 doentes diabéticos apresentaram média do ITB na artéria tibial posterior de 0,26 versus 0,28 dos 55 doentes não diabéticos (p = 0,6); e no ITB da artéria pediosa aqueles apresentaram média de 0,32 versus 0,23 desses (p = 0,06). Estratificando os doentes nas categorias da Classificação de Rutherford, não houve diferença nas médias do ITB nas categorias 4 e 5. Apenas em relação à artéria pediosa e em pacientes na Categoria 6, a média do ITB foi significativamente maior em diabéticos (0,44 versus 0,16; p = 0,03). Conclusão Os diabéticos apresentaram maior prevalência de ITB falsamente elevado. Porém, excluindo-se esses casos, a média dos valores de ITB são semelhantes aos não diabéticos, exceto na artéria pediosa, nos pacientes com isquemia na categoria 6.

Calcification of the arterial tunica media can falsely elevate the Ankle-Brachial Index (ABI) in diabetics, making it difficult to assess arterial disease. Objective To compare ABI values in diabetics and non-diabetics with critical ischemia. Methods A total of 140 patients (60% diabetics) with critical ischemia due to infrainguinal peripheral arterial obstructive disease were recruited from the vascular surgery service at the Complexo Hospitalar Universitário Professor Edgard Santos. Mean ABI values for the two groups of patients were compared and correlated with severity of ischemia, according to the Rutherford Classification. Statistical analysis was conducted using EPI-INFO. Results A majority of the 140 patients (77%) were classified as Rutherford Category 5, 6% as Category 4 and 17% as Category 6. Nine diabetics (11%) and one non-diabetic (2%) exhibited ABI > 1.15 (p = 0.02) and were excluded from the comparative analysis of mean ABIs. For the 130-patient sample, the 75 diabetic patients had a mean ABI for the posterior tibial artery of 0.26, vs. 0.28 for the 55 non-diabetic patients (p = 0.6); while mean ABIs for the dorsalis pedis artery were 0.32 vs. 0.23 respectively (p = 0.06). When the patients were stratified by Rutherford categories, there were no differences in mean ABIs in categories 4 or 5. Only mean ABI for the dorsalis pedis artery in Category 6 patients was significantly higher among diabetics (0.44 vs. 0.16; p = 0.03). Conclusions The diabetic patients had a higher prevalence of falsely elevated ABI, but when these cases were excluded, mean ABI values were similar to those of non-diabetic patients, with the exception of ABI measured at the dorsalis pedis artery in patients with category 6 ischemia.

Six-week anaerobic training improves proteolytic profile of diabetic rats

Objective To evaluate the effect of six-week anaerobic training on the mRNA expression of genes related to proteolysis Ubb (Ubiquitin), E2-14kDa, Trim63 (MuRF1 protein) and Nfkb1 in the skeletal muscle of diabetic rats.Materials and methods Four groups were established: DE (DiabetesExercised), DS (Diabetes Sedentary), CE (Control Exercised) and CS (Control Sedentary). The training consisted of 3 sets of 12 jumps in the liquid mean with load equivalent to 50% of BW for 6 weeks. Euthanasia occurred under ip anesthesia, and blood, adipose tissue and skeletal muscles were collected. Gene expression was quantified by RT–PCR in the gastrocnemius muscle. ANOVA one-way was used for comparison among groups, with post-hoc (Tukey) when necessary, considering p < 0.05.Results We observed reduction in the body weight and adipose tissue in the diabetic groups. The muscle mass was reduced in DS, which could be reversed by training (DE). Although DS and DE have presented similar body weight, the training protocol in DE promoted reduction in the adipose tissue, and increase of muscle mass. Anaerobic training was efficient to reduce glycaemia only in the diabetic animals until 6 hours after the end of training. The Trim63 gene expression was increased in DS; decreased Ubb gene level was observed in trained rats (CE and DE) compared to sedentary (CS and DS), and DE presented the lowest level of E2-14kDa gene expression.Conclusion Six-week anaerobic training promoted muscle mass gain, improved glycemic control, and exerted inhibitory effect on the proteolysis of gastrocnemius muscle of diabetic rats.