Innocuité d'une recette médicinale traditionnelle utilisée pour l'infertilité masculine en Côte d'Ivoire / Safety of a traditional medicinal recipe used for male infertility in Ivory Coast

L'infertilité masculine est incriminée dans 50% des cas d'infertilité du couple. C'estun réel problème de santé publique en Afrique.Le présent travail avaitpour objectif d'évaluer l'innocuité et la tolérabilitéd'une recette médicinaletraditionnelle utilisée pour la prise en charge del'infertilité masculine dans le District Autonome d'Abidjan/Côte d'Ivoire. Cette étude a été réalisée dans le cadre de la valorisation de la pharmacopée africaine.Le matériel végétal était constitué de la recette médicinale et le matériel biologiquede 42 volontaires de sexe masculinsélectionnés sur une base raisonnée à postériori.Les volontaires ont été soumis à un traitement sur troismois. Lesvariables étudiées étaient: la glycémie,l'urée, la créatinine, les transaminases, les triglycérides et le cholestérol total. Leseffets secondaireset indésirablesont été notifiés.L'âge moyen de la populationd'étudeétait de 41,78 ans (± 7,73). Aux termes des trois mois d'utilisation de la recette médicinale, l'analyse statistiquedes variables biochimiquesa révélé: la glycémiep = 0,12; les transaminases p = 0,75; la créatinine p = 0,26; l'urée p = 0,04; les triglycérides p =0,95et le cholestérol total p = 0,64contre p< 0,05.Le principal effet secondaire notifié était la polyurie (97,5%).Ces résultatsaffirmentla sécurité de la recette médicinalepourl'utilisateur. Ils encouragentson utilisation chez les hommes infertileset un suivimédicaldespersonnes sous antihypertenseur.La recette médicinale présente une bonne innocuité et une bonne tolérabilité

Male infertility is incriminated in 50% of cases of couple infertility. This is a real public health problem in Africa.The present work aimed to evaluate the safety and tolerability of a traditional medicinal recipe used to treatmale infertilitMale infertility is incriminated in 50% of cases of couple infertility. This is a real public health problem in Africa.The present work aimed aimed to the safety and tMale infertility is incriminated in 50% of cases of couple infertility. This is a real public health problem in Africa.The present work aimed to evaluate the safety and tolerability of a traditional medicinal recipe used to treatmale infertility in the Autonomous District of Abidjan/Côte d'Ivoire. This study was carried out as part of the promotion of the African pharmacopoeia.The plant material consisted of the medicinal recipe and the biological material of 42 male volunteers selected purposively asposteriori. The volunteers underwent treatment over three months. The variables studied wereblood sugar, urea, creatinine, transaminases, triglycerides and total cholesterol. Side effects and undesirable effects were reported.The average age of the study population was 41.78 years (± 7.73). After three months of use of the medicinal recipe, the statistical analysis ofbiochemical variables revealedblood sugar p = 0.12; transaminases p = 0.75; creatinine p = 0.26; urea p = 0.04; triglycerides p = 0.95 and total cholesterol p = 0.64 versus p < 0.05. The main side effect reported was polyuria (97.5%).These results affirm the safety of the medicinal recipe for the user. They encourage its use in infertile men and medical monitoring of people on antihypertensive medication.The medicinal recipe isgood safety and tolerability.

Saponins isolated from Schizocapsa plantaginea inhibit human hepatocellular carcinoma cell growth in vivo and in vitro via mitogen-activated protein kinase signaling / 中国天然药物

The underground cane of Schizocapsa plantaginea (Hance) has long been used by Chinese ethnic minority as a constituent of anti-cancer formulae. Saponins are abundant secondary metabolic products located in the underground cane of this plant. The potential therapeutic effects of total saponins isolated from Schizocapsa plantaginea (Hance) (SSPH) on human hepatocellular carcinoma (HCC) were tested in vitro in human liver cancer cell lines, SMMC-7721 and Bel-7404. Apoptosis and cell cycle arrest were determined using flow cytometry, caspase activation was determined by ELISA, and PARP, cleaved PARP, mitogen-activated protein kinase (MAPK) expression and phosphorylation were measured using Western blotting analysis. In vivo anti-HCC effects of SSPH were verified in nude mouse xenograft model. SSPH exerted markedly inhibitory effect on HCC cell proliferation in time- and concentration-dependent manner. Moreover, SSPH significantly induced apoptosis through caspase-dependent signaling and arrested cell cycle at G/M phase. These anti-proliferation effects of SSPH were associated with up-regulated phosphorylation of extracellular signal-regulated kinase-1/2 (Erk1/2) and c-jun-NH2-kinase-1/2 (JNK1/2) and reduced phosphorylation of p38MAPK. Furthermore, inhibitors of ERK, UO126, and JNK, SP600125 inhibited the anti-proliferation effects by SSPH, suggesting that Erk and JNK were the effector molecules in SSPH induced anti-proliferative action. During in vivo experiments, SSPH was found to inhibit xenograft tumor growth in nude mice, with a similar mechanism in vitro. Our study confirmed that SSPH exerted antagonistic effects on human liver cancer cells both in vitro and in vivo. Molecular mechanisms underlying SSPH action might be closely associated with MAPK signaling pathways. These results indicated that SSPH has potential therapeutic effects on HCC.

Saponins isolated from Schizocapsa plantaginea inhibit human hepatocellular carcinoma cell growth in vivo and in vitro via mitogen-activated protein kinase signaling / 中国天然药物

The underground cane of Schizocapsa plantaginea (Hance) has long been used by Chinese ethnic minority as a constituent of anti-cancer formulae. Saponins are abundant secondary metabolic products located in the underground cane of this plant. The potential therapeutic effects of total saponins isolated from Schizocapsa plantaginea (Hance) (SSPH) on human hepatocellular carcinoma (HCC) were tested in vitro in human liver cancer cell lines, SMMC-7721 and Bel-7404. Apoptosis and cell cycle arrest were determined using flow cytometry, caspase activation was determined by ELISA, and PARP, cleaved PARP, mitogen-activated protein kinase (MAPK) expression and phosphorylation were measured using Western blotting analysis. In vivo anti-HCC effects of SSPH were verified in nude mouse xenograft model. SSPH exerted markedly inhibitory effect on HCC cell proliferation in time- and concentration-dependent manner. Moreover, SSPH significantly induced apoptosis through caspase-dependent signaling and arrested cell cycle at G/M phase. These anti-proliferation effects of SSPH were associated with up-regulated phosphorylation of extracellular signal-regulated kinase-1/2 (Erk1/2) and c-jun-NH2-kinase-1/2 (JNK1/2) and reduced phosphorylation of p38MAPK. Furthermore, inhibitors of ERK, UO126, and JNK, SP600125 inhibited the anti-proliferation effects by SSPH, suggesting that Erk and JNK were the effector molecules in SSPH induced anti-proliferative action. During in vivo experiments, SSPH was found to inhibit xenograft tumor growth in nude mice, with a similar mechanism in vitro. Our study confirmed that SSPH exerted antagonistic effects on human liver cancer cells both in vitro and in vivo. Molecular mechanisms underlying SSPH action might be closely associated with MAPK signaling pathways. These results indicated that SSPH has potential therapeutic effects on HCC.

Composition and property characterization of Rhizoma Dioscoreae polysaccharide-Fe (III) complex / 中国中药杂志

<p><b>OBJECTIVE</b>To characterize properties of Rhizoma Dioscoreae polysaccharide-Fe (III) complex (RDPC) and analyze its composition.</p><p><b>METHOD</b>Physicochemical properties, such as character, solubility and stability of RDPC were studied. Surface structure of RDPC was studied by Infrared spectroscopic technique and transmission electron microscopy. The content of Fe (III) in RDPC was determined by o-phenanthroline spectrophotometry after being treated by ashing, wet digestion, or direct dissolution methods. Content of polysaccharide in RDPC was determined by sulphuric acid-phenol method.</p><p><b>RESULT</b>RDPC was brownish red powder. It could dissolve in water, its aqueous solution was very stable at the pH from 3.8 to 11.6. RDPC was a surface complex in which polysaccharide combined with beta-FeOOH as core. The content of Fe (III) in RDPC was 17.05%, 16.53%, 17.10% respectively after being treated by 3 different pretreatment methods. Content of polysaccharide in RDPC was 16.27%.</p><p><b>CONCLUSION</b>This is the first report of RDPC which was stable under physiological pH conditions and could be a new candidate for iron-supplementary.</p>