RESUMO
Newcastle disease vaccines
Assuntos
Animais , Embrião de Galinha , Galinhas/virologia , Vírus da Doença de Newcastle/patogenicidade , Doenças das Aves Domésticas/prevenção & controle , Vacinas Atenuadas/uso terapêutico , Vacinas Virais/uso terapêutico , Técnicas de Cultura de Células , Células Cultivadas , Galinhas/imunologia , Vírus da Doença de Newcastle/classificação , Vírus da Doença de Newcastle/crescimento & desenvolvimento , Cultura Primária de Células , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/virologia , Vacinação , Vacinas Atenuadas/efeitos adversos , Vacinas Atenuadas/imunologia , Vacinas Virais/efeitos adversos , Vacinas Virais/imunologiaRESUMO
Salmonella enterica Gallinarum (SG) causes fowl typhoid (FT), a septicemic disease in avian species. We constructed deletion mutants lacking the stress sigma factor RpoS, the nitric oxide (NO)-detoxifying flavohemoglobin Hmp, and the SsrA/SsrB regulator to confirm the functions of these factors in SG. All gene products were fully functional in wild-type (WT) SG whereas mutants harboring single mutations or a combination of rpoS, hmp, and ssrAB mutations showed hypersusceptibility to H2O2, loss of NO metabolism, and absence of Salmonella pathogenicity island (SPI)-2 expression, respectively. A triple-deletion mutant, SGDelta3 (SGDeltarpoSDeltahmpDeltassrAB), was evaluated for attenuated virulence and protection efficacy in two-week-old Lohmann layer chickens. The SGDelta3 mutant did not cause any mortality after inoculation with either 1 x 10(6) or 1 x 10(8) colony-forming units (CFUs) of bacteria. Significantly lower numbers of salmonellae were recovered from the liver and spleen of chickens inoculated with the SGDelta3 mutant compared to chickens inoculated with WT SG. Vaccination with the SGDelta3 mutant conferred complete protection against challenge with virulent SG on the chickens comparable to the group vaccinated with a conventional vaccine strain, SG9R. Overall, these results indicate that SGDelta3 could be a promising candidate for a live Salmonella vaccine against FT.
Assuntos
Animais , Feminino , Administração Oral , Proteínas de Bactérias/genética , Galinhas , Doenças das Aves Domésticas/imunologia , Salmonelose Animal/imunologia , Vacinas contra Salmonella/administração & dosagem , Salmonella enterica/imunologia , Vacinas Atenuadas/administração & dosagem , VirulênciaRESUMO
The immune response elicited by the oral inoculation of an intermediate strain of infectious bursal disease virus was studied in chickens. A strong over expression of IL-6, IL-8, IFNα and IFNγ was observed in bursa at 3 days post inoculation together with an increase in splenic NO2 release. An influx of T-lymphocytes was also detected.
Assuntos
Animais , Infecções por Birnaviridae/veterinária , Galinhas , Vírus da Doença Infecciosa da Bursa/imunologia , Doenças das Aves Domésticas/imunologia , Administração Oral , Infecções por Birnaviridae/imunologia , Bolsa de Fabricius/patologia , Citocinas/análise , Citocinas/genética , Perfilação da Expressão Gênica , Óxido Nítrico/análise , Baço/patologia , Linfócitos T/imunologiaRESUMO
The critical time of avian leukosis virus subgroup J (ALV-J)-mediated immunosuppression was determined by body weight, relative immune organ weight, histopathology, and presence of group specific antigen and antibodies in specific pathogen-free (SPF) chickens. CD4+ and CD8+ cell activity in the spleen, total and differential leukocyte counts in blood, and viral RNA levels in spleen were measured. Significant growth suppression was observed in the two ALV-J-infected groups. A strong immune response by infected groups was present in spleen at 2-weeks-of-age, but after 4-weeks-of-age, the response decreased quickly. The thymus and bursa showed persistent immunosuppression until 4-weeks-of-age. Proliferation of fibroblasts and dendritic cells were observed in immune organs at 4- and 5-weeks-of-age. However, the granulocyte cell number was markedly lower in the infected groups than in the control group. In group 1 (day 1 infection) CD4+ cells increased during the second week but significantly decreased during the fourth week, while group 2 (day 7 infection) showed the opposite effect. Viral RNA increased significantly by the fourth week. These data identify 3~4 weeks post-infection as the key time at which the ALV-J virus exerts its immunosuppressive effects on the host.
Assuntos
Animais , Anticorpos Antivirais/sangue , Antígenos CD4/sangue , Antígenos CD8/sangue , Leucose Aviária/imunologia , Vírus da Leucose Aviária/classificação , Peso Corporal , Galinhas , China , Ensaio de Imunoadsorção Enzimática/veterinária , Tolerância Imunológica , Contagem de Leucócitos/veterinária , Doenças das Aves Domésticas/imunologia , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Organismos Livres de Patógenos Específicos , Baço/imunologiaRESUMO
Newcastle disease (ND) caused by virulent Newcastle disease virus (NDV) is a highly contagious viral disease of poultry. Virulent NDVs characteristically have a multibasic amino acid sequence (virulence motif) such as (112)RRQKRF(117) at the cleavage site of the precusor fusion (F0) protein. The antigenic and immunogenic characteristics of the virulence motif (112)RRQKRF(117) in the F0 protein of virulent NDVs were investigated. Epitope mapping analysis revealed that a RRQKRF-specific monoclonal antibody 4G2 recognized the KRF section of the motif. A synthetic peptide bearing the RRQKRF motif reacted strongly with sera from virulent NDV (with RRQKRF motif)-infected chickens. These sera also showed reactivity to peptides bearing other virulence motifs ((112)KRQKRF(117), (112)RRQRRF(117) and (112)RRRKRF(117)) but not an avirulence motif ((112)GRQGRL(117)) by ELISA. The synthetic bearing RRQKRF motif reacted with 60% to 91% of sera taken from surviving chickens on ND outbreak farms but not with sera from vaccinated birds, even though most of the sera had antibody to NDV due to vaccination. This indicates that the virulence motif has the potential to differentiate virulent NDV infected birds from vaccinated birds.
Assuntos
Animais , Motivos de Aminoácidos/imunologia , Sequência de Aminoácidos , Galinhas , Ensaio de Imunoadsorção Enzimática/veterinária , Mapeamento de Epitopos/veterinária , Doença de Newcastle/imunologia , Vírus da Doença de Newcastle/genética , Doenças das Aves Domésticas/imunologia , Testes Sorológicos/veterinária , Proteínas Virais de Fusão/genética , Virulência/genéticaRESUMO
Avian metapneumovirus (aMPV) causes upper respiratory tract infections in chickens and turkeys. Although the swollen head syndrome (SHS) associated with aMPV in chickens has been reported in Korea since 1992, this is the study isolating aMPV from chickens in this country. We examined 780 oropharyngeal swab or nasal turbinate samples collected from 130 chicken flocks to investigate the prevalence of aMPV and to isolate aMPV from chickens from 2004-2008. Twelve aMPV subtype A and 13 subtype B strains were detected from clinical samples by the aMPV subtype A and B multiplex real-time reverse transcription polymerase chain reaction (RRT-PCR). Partial sequence analysis of the G glycoprotein gene confirmed that the detected aMPVs belonged to subtypes A and B. Two aMPVs subtype A out of the 25 detected aMPVs were isolated by Vero cell passage. In animal experiments with an aMPV isolate, viral RNA was detected in nasal discharge, although no clinical signs of SHS were observed in chickens. In contrast to chickens, turkeys showed severe nasal discharge and a relatively higher titer of viral excretion than chickens. Here, we reveal the co-circulation of aMPV subtypes A and B, and isolate aMPVs from chicken flocks in Korea.
Assuntos
Animais , Anticorpos Antivirais/sangue , Sequência de Bases , Galinhas , Glicoproteínas/química , Metapneumovirus/imunologia , Dados de Sequência Molecular , Infecções por Paramyxoviridae/imunologia , Filogenia , Doenças das Aves Domésticas/imunologia , RNA Viral/química , Infecções Respiratórias/imunologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Alinhamento de Sequência , Análise de Sequência de DNA , Sorotipagem , Organismos Livres de Patógenos Específicos , PerusRESUMO
The aim of this study was to examine the efficacy of in ovo prime-boost vaccination against infectious bursal disease virus (IBDV) using a DNA vaccine to prime in ovo followed by a killed-vaccine boost post hatching. In addition, the adjuvant effects of plasmid-encoded chicken interleukin-2 and chicken interferon-gamma were tested in conjunction with the vaccine. A plasmid DNA vaccine (pcDNA-VP243) encoding the VP2, VP4, and VP3 proteins of the very virulent IBDV (vvIBDV) SH/92 strain was injected into the amniotic sac alone or in combination with a plasmid encoding chicken IL-2 (ChIL-2) or chicken IFN-gamma (ChIFN-gamma) at embryonation day 18, followed by an intramuscular injection of a commercial killed IBD vaccine at 1 week of age. The chickens were orally challenged with the vvIBDV SH/92 strain at 3 weeks of age and observed for 10 days. In ovo DNA immunization followed by a killed-vaccine boost provided significantly better immunity than the other options. No mortality was observed in this group after a challenge with the vvIBDV. The prime-boost strategy was moderately effective against bursal damage, which was measured by the bursa weight/body weight ratio, the presence of IBDV RNA, and the bursal lesion score. In ovo DNA vaccination with no boost did not provide sufficient immunity, and the addition of ChIL-2 or ChIFN-gamma did not enhance protective immunity. In the ConA-induced lymphocyte proliferation assay of peripheral blood lymphocyte collected 10 days post-challenge, there was greater proliferation responses in the DNA vaccine plus boost and DNA vaccine with ChIL-2 plus boost groups compared to the other groups. These findings suggest that priming with DNA vaccine and boosting with killed vaccine is an effective strategy for protecting chickens against vvIBDV.
Assuntos
Animais , Embrião de Galinha , Adjuvantes Imunológicos/farmacologia , Anticorpos Antivirais/sangue , Infecções por Birnaviridae/imunologia , Peso Corporal/imunologia , Bolsa de Fabricius/imunologia , Galinhas , Histocitoquímica/veterinária , Imunização/veterinária , Vírus da Doença Infecciosa da Bursa/genética , Interferon gama/farmacologia , Interleucina-2/farmacologia , Tamanho do Órgão/imunologia , Doenças das Aves Domésticas/imunologia , RNA Viral/química , Distribuição Aleatória , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Organismos Livres de Patógenos Específicos , Vacinas de DNA/administração & dosagem , Vacinas de Produtos Inativados/administração & dosagem , Vacinas Virais/administração & dosagemRESUMO
Despite the intensive vaccination policy that has been put in place to control Newcastle disease virus (NDV), the recent emergence of NDV genotype VII strains in Korea has led to significant economic losses in the poultry industry. We ssessed the ability of inactivated, oil-emulsion vaccines derived from La Sota or Ulster 2C NDV strains to protect chickens from challenge with Kr-005/00, which is a recently isolated Korean epizootic genotype VII strain. Six-week-old SPF chickens were vaccinated once and challenged three weeks later via the eye drop/intranasal route. All vaccinated birds were fully protected from disease, regardless of the vaccine strains used. All vaccinated and challenged groups showed significant sero-conversion 14 days after challenge. However, some vaccinated birds, despite being protected from disease, shed the challenge virus from their oro-pharynx and cloaca, albeit at significantly lower titers than the unvaccinated challenged control birds. The virological, serological, and epidemiological significance of our observations with regard to NDV disease eradication is discussed.
Assuntos
Animais , Administração Intranasal , Galinhas , Cloaca/virologia , Surtos de Doenças/prevenção & controle , Coreia (Geográfico) , Doença de Newcastle/imunologia , Vírus da Doença de Newcastle/imunologia , Soluções Oftálmicas , Doenças das Aves Domésticas/imunologia , Vacinas de Produtos Inativados/administração & dosagem , Vacinas Virais/administração & dosagem , Eliminação de Partículas Virais/efeitos dos fármacosRESUMO
Infectious bursal disease (IBD) is an acute and highly contagious disease of young chickens caused by Birnavirus. Mortality of infected birds can be best prevented if injected with antibodies. The present study was an attempt to raise specific hyper-immune polyclonal antibodies against IBD virus in Pakistan. Commercial layers divided into four groups were injected with IBD vaccine subcutaneously according to four different treatment regimens. Eggs were collected daily and antibodies were purified from yolk with dextran sulphate. Titers of antibodies in serum and yolk were evaluated with enzyme linked immunosorbant assay and agar gel precipitation test. Antibody titers were significantly higher in yolk than serum. Eggs collected at 28 days post-vaccination had maximum antibody titers. Of treatment regimens, T3 was found to be most effective for hyperimmunization. Lyophilized antibodies stored at 4oC did not lose their activity till the end of experiment. IBD virus infected birds were injected with purified antibodies which induced 92% recovery as compared to control birds. The study implicates that the purified antibodies may be useful as a therapeutic agent to cure IBD infected birds.
Assuntos
Animais , Feminino , Anticorpos Antivirais/sangue , Infecções por Birnaviridae/imunologia , Galinhas , Gema de Ovo/imunologia , Ensaio de Imunoadsorção Enzimática/veterinária , Imunização/métodos , Imunoglobulinas/imunologia , Imunoterapia/métodos , Vírus da Doença Infecciosa da Bursa/imunologia , Doenças das Aves Domésticas/imunologia , Testes de Precipitina/veterinária , Vacinas Virais/imunologiaRESUMO
This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxynucleotides (CpG-ODN), and chicken interferon-gamma (ChIFN-gamma) on a DNA vaccine (pcDNA-VP243) against the infectious bursal disease virus (IBDV). A plasmid encoding chicken IFN-atilde was constructed. Twice at 2-week intervals, twoweek-old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2 after the second immunization, the chickens were orally challenged with the highly virulent IBDV. The groups that received the DNA vaccines plus either DDA or CpG-ODN showed significantly lower survival rates than the group that received the DNA vaccine alone. However, the survival rates for the DNA vaccine alone and for the DNA vaccine plus ChIFN-gamma were similar. The chickens had no detectable antibodies to the IBDV before the challenge but all the surviving chickens in all groups except for the normal control group showed the induction of antibodies to the IBDV at day 10 after the challenge. As judged by the lymphocyte proliferation assays using the a WST-8 solution performed on the peripheral blood and splenic lymphocytes, the stimulation indices (SI) of the peripheral blood lymphocytes in all groups except for the normal control group were similar immediately before the challenge. At 10 days post-challenge, the SI for DNA vaccine plus either CpG-ODN or ChIFN-gamma was similar to that of the DNA vaccine control group. For splenic lymphocytes, the SI in the DNA vaccine plus CpG-ODN and DNA vaccine plus ChIFN-gamma groups were higher than for the DNA vaccine control. These results suggest that DDA actually compromises the protection against the IBDV by DNA vaccine, and CpG-ODN and IFN-gamma had no significant effect.
Assuntos
Animais , Adjuvantes Imunológicos , Anticorpos Antivirais/sangue , Infecções por Birnaviridae/imunologia , Bolsa de Fabricius/imunologia , Proliferação de Células , Galinhas , Ilhas de CpG/imunologia , Ensaio de Imunoadsorção Enzimática/veterinária , Imunização/métodos , Vírus da Doença Infecciosa da Bursa/imunologia , Interferon gama/imunologia , Linfócitos/citologia , Oligonucleotídeos/imunologia , Doenças das Aves Domésticas/imunologia , Organismos Livres de Patógenos Específicos , Vacinas de DNA/imunologia , Vacinas Virais/imunologiaRESUMO
The ability of a heat-inactivated whole virus from a highly virulent infectious bursal disease virus (hvIBDV) and VP2 protein from hvIBDV expressed in E. coli provided protection against a hvIBDV challenge in specificpathogen- free (SPF) chickens. Six out of seven chickens that were injected three times with crude VP2 protein developed significant antibody titer against IBDV. However, only four out of the seven chickens survived the hvIBDV challenge. Despite showing low antibody titer profiles, all chickens immunized with the heat-inactivated whole virus also survived the challenged with hvIBDV. However, all of these chickens had bursal atrophy and mild to moderate depletion of lymphocytes. Thus, antibodies raised against IBDV VP2 protein expressed in E. coli and denatured IBDV proteins induced some degree of protection against mortality but not against bursal damage following challenge with hvIBDV.
Assuntos
Animais , Anticorpos Antivirais/sangue , Infecções por Birnaviridae/imunologia , Galinhas , Ensaio de Imunoadsorção Enzimática/veterinária , Escherichia coli/genética , Imunização/normas , Vírus da Doença Infecciosa da Bursa/genética , Doenças das Aves Domésticas/imunologia , Proteínas Recombinantes/genética , Organismos Livres de Patógenos Específicos , Vacinas Atenuadas/imunologia , Vacinas Sintéticas/imunologia , Proteínas Estruturais Virais/biossíntese , Vacinas Virais/imunologiaRESUMO
An attempt was made to evaluate the protective efficacy of maternal antibodies in chicks against salmonellosis. Layer chicks ageing 21 days were individually vaccinated with 100 microg of Salmonella enterica subspecies enterica serovar Weltevreden (BM 1643) toxoid adjuvanted with vitamin E subcutaneously. After 90 days of the primary vaccination the birds were given booster dose of the vaccine. The saline extract of the yolk of eggs laid by the vaccinated birds yielded agglutination and ELISA titres ranging from 43.2 +/- 5.33 to 75.2 +/- 6.26 and 4.987 x 10(3) +/- 0.54 to 5.89x103 +/- 0.56, respectively. Sera of chicks hatched from eggs laid by the vaccinated layers were also subjected to agglutination and ELISA. Agglutination and ELISA titres on the 5th day--post hatching (dph) were 21.6 +/- 1.75 and 4.025 x 10(2) +/- 0.59, while on the 10th dph titers were 13.6 +/- 1.65 and 1.21 x 10(2) +/- 0.60, respectively. It was also observed that only one out of 6 chicks died when challenged with 2 x 10(9) CFU of S. serovar Gallirarum at the age of 7 days showing 83.33% protection. Thus it can be concluded that passive immunity confided by Salmonella enterica subspecies enterica serovar Weltevreden (BM 1643) toxoid can protect chicks against salmonellosis during their early days of life.
Assuntos
Animais , Anticorpos Antibacterianos/imunologia , Galinhas/imunologia , Imunidade Materno-Adquirida , Doenças das Aves Domésticas/imunologia , Salmonelose Animal/imunologia , Vacinas contra Salmonella/administração & dosagem , Salmonella enterica/imunologia , Toxoides/imunologiaRESUMO
In order to investigate if there is any definite correlation between the degree of T-cell response in the bursa of Fabricius (BF) and the virulence of Infectious Bursal Disease (IBD) virus strains, chickens were infected with strains of different virulence i.e. mild (Lukert strain), intermediate (Georgia strain) or invasive intermediate (IV-95 strain). At various times post-inoculation, bursal samples were collected to study virus specific histopathological lesions, the distribution of viral antigen and the extent of T-cell infiltration in the bursa. Most severe bursal lesions were induced by IV-95 strain (the invasive intermediate strain), whereas Lukert, the mild strain caused the least severe lesions. The number of virus positive cells in the bursa was highest in chickens infected with IV-95 strain. Substantial infiltration of CD4+ and CD8+ T-cells in the bursal follicles of virus-infected groups was observed from 4 d.p.i. onwards. The magnitude of T-cell response was more in the birds infected with intermediate (Georgia) or invasive intermediate strains of virus than chickens inoculated with mild (Lukert) strain, even when 10-fold higher doses of the inoculums were used. PHA responses to peripheral lymphocytes were found suppressed in all the groups of chickens only transiently. The results indicate that the magnitude of T-cell responses in BF during IBDV infection is influenced more by the virulence of virus strain rather than the quantum of viral load in BF. Over all these studies may have implications in understanding the role of T-cells in pathogenesis and immunity in IBD.
Assuntos
Animais , Infecções por Birnaviridae/imunologia , Bolsa de Fabricius/imunologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Galinhas , Vírus da Doença Infecciosa da Bursa/classificação , Doenças das Aves Domésticas/imunologia , Especificidade da Espécie , VirulênciaRESUMO
Studies were performed to determine the effects of Bcell suppression on the pathogenesis of Subgroup J avian leukosis virus (ALV-J) in broiler chickens. Neonatal chickens were treated with cyclophosphamide (CY) or PBS, and then infected with ALV-J (ADOL-7501) at 2 weeks of age. CY treatment induced B cell specific immunosuppression throughout the experiment confirmed by decreased bursal weight, intact lymphocyte mitogenetic activity stimulated by Con A and increased relative subpopulation of CD3-positive cells as measured by flow cytometry. Chickens in this experiment had Mareks disease virus exposure prior to three weeks of age as determined by the presence of lymphocytic infiltration and antibody. Virus neutralizing antibody against ALV-J was first observed at 6 weeks post-infection in some of the infected chickens in the PBS group. As expected, none of the chickens from the CY group and uninfected chickens developed virus-neutralizing antibody. The viremic status was measured by real time RT-PCR using SYBR green I dye. The percentage of viremic chickens was significantly higher, and more chickens had high titered viremia, in the CY treated group. No neoplastic foci consistent with ALVJ infection were observed in any of the experimental chickens. The frequency and intensity of viral antigen expression determined by immunohistochemistry was significantly higher in tissues from CY treated birds than those of PBS treated chickens at 3 weeks post-infection. This study showed that B cell specific immunosuppression with CY treatment in chickens resulted in increase in viremia and viral antigen load in tissues.
Assuntos
Animais , Leucose Aviária/imunologia , Vírus da Leucose Aviária/genética , Peso Corporal/fisiologia , Bolsa de Fabricius/imunologia , Galinhas , Concanavalina A/imunologia , Ciclofosfamida/farmacologia , Citometria de Fluxo/veterinária , Hospedeiro Imunocomprometido , Imuno-Histoquímica/veterinária , Imunofenotipagem/veterinária , Imunossupressores/farmacologia , Ativação Linfocitária/efeitos dos fármacos , Compostos Orgânicos/química , Doenças das Aves Domésticas/imunologia , RNA Viral/química , Distribuição Aleatória , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Baço/imunologia , Estatísticas não Paramétricas , Viremia/veterináriaRESUMO
Experiments were conducted in chickens to understand the effects of oral immunomodulation. Heat inactivated M phlei, a commensal Mycobacterium and a non-specific immunomodulator, was administered orally prior to live Newcastle disease F (ND F) strain vaccination. In experimental birds it lead to an enhanced cell mediated Immune response (CMI) against the vaccine. There was a reduction in the Haemagglutination inhibiting (HI) antibodies. However, it did not affect the protection against a virulent challenge, as the protection percentage was more or less same in vaccinated birds irrespective of the M.phlei administration. M. phlei administration could not enhance the immune response to inactivated ND F vaccine administered orally. The results indicate that M. phlei favours a CMI response to orally administered live ND F vaccine. It may be of potential use in enhancing CMI against vaccines and a cheaper alternative to costlier recombinant cytokines.
Assuntos
Adjuvantes Imunológicos/administração & dosagem , Administração Oral , Animais , Anticorpos Antivirais/análise , Formação de Anticorpos , Galinhas/imunologia , Feminino , Formazans/diagnóstico , Testes de Inibição da Hemaglutinação , Imunidade Celular , Masculino , Mycobacterium phlei/imunologia , Doença de Newcastle/imunologia , Vírus da Doença de Newcastle/imunologia , Doenças das Aves Domésticas/imunologia , Sais de Tetrazólio/diagnóstico , Vacinação/veterinária , Vacinas Virais/administração & dosagemRESUMO
Con la finalidad de evaluar el interferón (INF) secretado durante la infección experimental primaria con Eimeria tenella se realizaron dos estudios. En el primero se evaluó la respuesta primaria en linfacitos T (LT) esplénicos de 40 pollos de engorda (Hubbard X Hubbard) de 20 días de edad, desafiados con 1 x 10 a la cuarta ooquistes esporulados de E. tenella. Los LT se aislaron a partir de cuatro bazos en dos mezclas celulares con la técnica de columnas de fibra de nailón a los días 1, 3, 5, 7 y 9 posinoculación (pi). En el segundo experimento de respuesta primaria, el aislamiento de los LT se efectuó con la técnica de Ficoll Hypaque con cultivos celulares esplénicos individuales provenientes de 30 aves. En cada uno de los dos experimentos se utilizó la mitad de aves del grupo desafiado como aves testigo sin desafiar. Para evidenciar el IFN presente cada día pi se empleó un ensayo antiviral con el virus de la enfermedad de Newcastle y la infección de la bolsa de Fabricio. En el primer experimento se observó que el IFN del grupo desafiado alcanzó un pico al día 5 pi que difirió (P< 0.05) del grupo testigo, bajó ligeramente al día 7 pi (P< 0.05) y se incrementó al día 9 pi (P< 0.05). En el segundo experimento, se determinó un comportamiento similar al primero, pero con títulos más altos. El patrón de liberación de IFN de los LT estimulados in vitro después del desafío con E. tenella es diferente al patron de liberación de los LT estimulado in vitro pero sin el desafío con E. tenella. Los ensayos antivirales utilizados detectaron cualitativa y cuantitativamente el IFN aviar secretado por LT estimulados in vitro. Los patrones de liberación de IFN detectado con las dos técnicas de aislamiento de LT después de la infección primaria con E. tenella se comportaron de manera semejante
Assuntos
Animais , Doenças das Aves Domésticas/imunologia , Linfócitos T/imunologia , Linfócitos T , Interferons/imunologia , Interferons , Eimeria tenella/imunologia , Ativação Viral , Imunidade CelularRESUMO
A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88 per cent), specificity (100 per cent) and agreement (95.31 per cent).
Assuntos
Animais , Anticorpos Antivirais/isolamento & purificação , Vírus da Bronquite Infecciosa/imunologia , Galinhas , Ensaio de Imunoadsorção Enzimática/métodos , Doenças das Aves Domésticas/imunologia , Sensibilidade e EspecificidadeRESUMO
Se aislaron tres virus de la infección de la bolsa de Fabricio (VIBF) a partir de muestras obtenidas de muestras en granjas avícolas en México, aquéllos se denominaron H, P y G. Se determinaron sus propiedades antigénicas mediante la técnica de ELISA por captura de antígenos, utilizando los anticuerpos monoclonales (Mab) B-29, R-63, BK-9, B-69 y 57. Se evaluó su virulencia en aves inoculadas mediante la observación de signos clínicos, mortalidad, proporción del peso bursal con el peso corporal, lesiones microscópicas, persistencia de los virus en tejidos linfoides, serología contra VIBF y medición de la respuesta inmune hacia Brucella abortus y eritrocitos de ovino. Los virus P y G presentaron los epítopes reconocidos por lo B-29, R-63 y B-69, que están presentes en las cepas clásicas de VIBF, el virus H no pudo ser reconocido por ningún Mab. Los tres aislamientos indujeron la presentación subclínica de la IBF en las aves inoculadas, se observó marcada atrofia bursal con deplesión linfoide severa, persistencia del VIBF en bolsa hasta los catorce días posinoculación y seroconversión de las aves a partir de la segunda semana después de la inoculación. A pesar de que no se detectó plenamente un estado de inmunodepresión mediante la respuesta a los dos antígenos utilizados, no se puede descartar un eventual efecto inmunodepresor por el aislamiento "H". Tampoco se detectaron diferencias en la virulencia de los tres VIBF aislados
Assuntos
Animais , Bolsa de Fabricius/virologia , Galinhas/virologia , Infecções por Birnaviridae/fisiopatologia , Infecções por Birnaviridae/mortalidade , Infecções por Birnaviridae/patologia , Birnaviridae/isolamento & purificação , Birnaviridae/patogenicidade , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/virologia , Ensaio de Imunoadsorção Enzimática , Anticorpos Monoclonais , Antígenos ViraisRESUMO
Effects of feeding of powdered dry leaves of A. indica (AI) were investigated on humoral and cell mediated immune responses, in a flock of broilers which had survived an outbreak of infectious bursal disease (IBD). AI (2 g/kg) treatment significantly enhanced the antibody titres against new castle disease virus (NCDV) antigen and also potentiated the inflammatory reactions to 1. Chloro-2,4-di- nitro benzene (DNCB) inskin contact test. The results indicate that AI could be beneficial in immunosuppressed condition like IBD, in poultry.
Assuntos
Adjuvantes Imunológicos/farmacologia , Animais , Formação de Anticorpos , Infecções por Birnaviridae/imunologia , Galinhas , Imunidade Celular , Vírus da Doença Infecciosa da Bursa , Plantas Medicinais , Doenças das Aves Domésticas/imunologiaRESUMO
Se utilizaron 50 pollitos de engorda de un día de edad que fueron distribuidos aleatoriamente en 3 grupos con 15 aves cada uno, más 5 para muestreo bacteriológico. A los 17 días de edad, se inoculó en promedio 2 ml de solución salina fosfatada estéril, por vía endovenosa a las aves del grupo 1, mientras que en los grupos 2 y 3 se les administró 5-fluorouracilo (5-FU) en solución a dosis de 200 y 300 mg/kg de peso, respectivamente. A 10 aves de cada grupo se les tomó una muestra sanguínea durante los días 1, 3, 5, 6, 7, 8, 9, 10, 12 y 15 postratamiento (PT) para realizar un conteo total y diferencial de leucocitos; posteriormente se obtuvo la proporción de leucocitos polimorfonucleares/leucocitos totales (PMN/L T). Los resultados obtenidos en este estudio mostraron que la proporción de PMN/L T en los grupos tratados con 5-FU disminuyó a partir del día 1 hasta el día 9 PT, los valores más bajos se presentaron al día 9 PT y los valores normales se recuperaron hasta el día 15 PT. Se encontraron diferencias altamente significativas (P< 0.001) entre grupos, entre los días de muestreo, así como en la interacción de la dosis de 5-FU y el tiempo PT en relación con los valores de las proporciones de PMN/L T. Asimismo, se observaron diferencias (P< 0.05) entre las proporciones de PMN/L T de las aves tratadas con 5-FU en comparación con las aves del grupo testigo, así como entre los días de muestreo en los grupos tratados. Las aves tratadas con 300 mg de 5.FU/kg de peso presentaron signos de toxicidad