RESUMO
Based on its traditional uses in folk medicine, the whole flower extract of Mesua ferrea Linn. was tested for its in vitro antimicrobial efficacy against five different strains of Salmonella spp. All the strains were found to be highly sensitive to the extract, MIC of the extract against each organism being 50 microg/ml. The extract was tested in vitro for its mode of antibacterial activity against S. Typhimurium NCTC 74 and it was found to be bactericidal in action. In vivo studies of this extract offered significant protection to Swiss albino mice at doses approximately 2 and 4 mg/mouse when challenged with 50 median lethal dose of S. Typhimurium NCTC 74. Further, the extract caused statistically significant reduction in viable count of the strain in liver, spleen and heart blood of challenged mice.
Assuntos
Animais , Anti-Infecciosos , Clusiaceae/metabolismo , Relação Dose-Resposta a Droga , Coração/microbiologia , Fígado/microbiologia , Camundongos , Estruturas Vegetais/metabolismo , Extratos Vegetais , Infecções por Salmonella/tratamento farmacológico , Salmonelose Animal/tratamento farmacológico , Salmonella typhimurium/metabolismo , Baço/microbiologia , Células-Tronco , VirulênciaRESUMO
The effect of ethanolic extract of Terminalia arjuna bark on carbohydrate metabolizing enzymes of N-nitrosodiethylamine induced hepatocellular carcinoma in Wistar albino rats were studied. The plasma and liver glycolytic enzymes such as hexokinase, phosphoglucoisomerase, aldolase were significantly increased in cancer induced animals while glyconeogenic enzyme, glucose-6-phosphatase was decreased. These enzymes were reverted significantly to near normal range in treated animals after oral administration of T. arjuna for 28 days. The modulation of the enzymes constitute the depletion of energy metabolism leads to inhibition of cancer growth. This inhibitory activity may be due to the anticancer activity of constituents present in the ethanolic extract of T. arjuna.
Assuntos
Animais , Metabolismo dos Carboidratos , Carcinoma Hepatocelular/induzido quimicamente , Dietilnitrosamina/farmacologia , Etanol/farmacologia , Fígado/metabolismo , Neoplasias Hepáticas/induzido quimicamente , Masculino , Fitoterapia/métodos , Casca de Planta/metabolismo , Estruturas Vegetais/metabolismo , Extratos Vegetais/farmacologia , Ratos , Ratos Wistar , Terminalia/metabolismoRESUMO
Carbon, hydrogen and nitrogen analyses of banana leaf and pseudostem biomass revealed their potentiality as substrates for microorganisms. Infra-red (IR) spectra of both biomass show presence of cellulose, xylan and lignin. IR spectra of leaf and pseudostem biomass degraded in solid state fermentation (SSF) by two Pleurotus species (P. sajor-caju and P. ostreatus) for 40 days showed the utilization of cellulose, xylan and lignin by these microbes. Dynamics of various lignocellulolytic enzymes of Pleurotus species and analyses of carbon, hydrogen and nitrogen contents of degraded biomass supported the same. Both the Pleurotus species exhibited lignin consumption ability on both the substrates.