Naftiluréia polisulfonada (Suramin) na inibição da migração, proliferação e formação de tubo vascular em culturas de células endoteliais coroidianas "in vitro" estimuladas por fatores de crescimento e em membrana coroidiana sub-retiniana experimental / Plysuldonated naphthylurea (Suramin) inhibeted migration, proliferation and the tube formation on coroidal endothelial cell culture

Avaliar o efeito do suramin na migração, proliferação e formação de tubo vascular em células endoteliais coroidianas (CECs) “in vitro” e em neovascularização coroidiana (NC) “in vivo”. Foi avaliada a migração através do experimento de Boyden Chamber. Foi avaliada a proliferação através do experimento MTT. Foi avaliada a formação de tubo vascular através do experimento gel colágeno 3D. As CECs foram estimuladas por fatores de crescimento (FC) e tratadas com suramin.O efeito sistêmico do suramin foi avaliado em NC induzidos por laser em olhos de ratos. O suramin inibiu a migração, proliferação e formação de tubo vascular estimulada por FC de forma dose dependente / This study evaluated the effects of suramin on choroidal endothelial cell (CEC) migration, proliferation and tube formation “in vitro” and choroidal neovascularization (CNV) “in vivo”. Migration was evaluated using Boyden chamber assay. Proliferation was evaluated by an MTT assay. Tube formation was evaluated using a 3D-tube formation assay. CECs were stimulated by growth factor (GF) treated with suramin. The effect of systemic administration of Suramin was evaluated on laser induced CNV in rats eyes. Suramin inhibited CEC migration, proliferation, and tube formation induced by GF in a dose dependent manner. CNV in rats was inhibited by systemic administration of Suramin 30mg/Kg. These studies indicate that suramin inhibits Angiogenesis “in vitro” and “in vivo”...

Clinical significance of microvessel density in multiple myeloma patients

To investigate the role of angiogenesis in multiple myeloma (MM), bone marrow biopsy from 75 adults with newly diagnosed, untreated MM were evaluated. Microvessels were scored in at least 3 areas ( x 200 fields) of the highest microvessel density in representative sections of each bone marrow specimen using immunohistochemistry for CD34. Prognostic variables were also evaluated for the overall survival. Microvessel counts were significantly higher in patients with MM (n=69.42+/-9.67), compared with control (n=26.81+/-2.85). Microvessel density had a weak correlation with percentage of bone marrow plasma cells. By univariate analysis, age, beta2-microglobulin, serum albumin, serum creatinine, serum calcium, hemoglobin, platelet count, and bone marrow plasma cell percentage were correlated with survival. By multivariate analysis, age, serum albumin, serum creatinine, hemoglobin, platelet count and bone marrow plasma cell percentage were correlated with overall survival, whereas microvessel density was not. In summary, microvessel density in bone marrow of MM is significantly increased compared to control, but was not correlated with overall survival. Further studies regarding angiogeneic molecules are needed to determine the functional role of angiogenesis in MM.

Correlation of VEGF with contrast enhancement on dual-phase dynamic helical CT in liver tumors: preliminary study

The purpose of this preliminary study is to elucidate that vascular endothelial growth factor (VEGF) influences contrast enhancement of hepatic tumors on computed tomography (CT). Fourteen patients with hepatic tumors (11 hepatocellular carcinomas; 3 metastatic cancers) underwent a dual-phase dynamic helical CT or computed tomographic hepatic arteriography. The attenuation of each mass was determined as hyperattenuation, isoattenuation or hypoattenuation with respect to the adjacent nontumorous parenchyma. Gun-needle biopsy was done for each tumor, and paraffin sections were immunostained with anti- VEGF antibody by the avidin-biotin-peroxidase complex method. The pathologic grade was made by intensity (1 +, 2+, 3+) and area (+/-, 1 +, 2+). The tumor ranged 2.0-14.0 cm in size (mean, 5.8 cm). In arterial phase, the intensity was not correlated with the degree of enhancement (p=0.086). However, the correlation between the attenuation value of hepatic arterial phase and the area of positive tumor cells was statistically significant (p=0.002). VEGF may be the factor that enhances the hepatic mass with water-soluble iodinated contrast agent in CT.

The application of vascular endothelial growth factor in forensic science / 法医学杂志

Vascular Endothelial Growth Factor (VEGF) is a potent angiogenic factor with a key role in several pathological processes, including wound repair as well as a effective vascular permeability factor. This article review the present study of VEGF in molecular biology, the connection with repair and expression regulation and so on.

Angiogenesis: characterization of a cellular model

Endothelial cell proliferation and differentiation into blood capillaries (i.e., angiogenesis) are essential for growth and development, wound healing, osetogenesis, etc. But abnormal angiogenesis during tumor progression could lead to serious consequences. Angiogenesis is a complex biochemical process, and is often difficult to study the molecular mechanism in vivo due to interference by multitude of factors. Here, I present a non-transformed capillary endothelial cell line as a model which has been extensively characterized morphologically and biochemically to study the fundamentals of the angiogenic process. Studies completed in our laboratory also evidenced that expression of Glc3Man9GlcNAc2-PP-Dol is intricately connected with the balance between the cellular proliferation and apoptosis during angiogenesis.