Otomycosis; a clinicomycological study and efficacy of tincture mertheolate in it's treatment

To determine the efficacy of tincture mertheolate in otomycosis. An experimental study. Department of ENT Aliama Iqbal Medical College/ Jinnah Hospital, Lahore. From Dec. 2007 to April 2008. A total of 60 patients of symptomatic otomycosis were investigated prospectively. Aural swabs were collected on first, 7[th] and 14[th] day and examined, by direct microscopy of external auditory canal and culture for fungi. Of these 60 patients found to be having pure fungal infection were taken up for mycological and therapeutic study. Fungi belonging to Aspergiilus were isolated in 57 [95%] patients of which Aspergillus niger was the commonest isolated in 34[56.6%] patients followed by Aspergillus flavus in 21[35%] and Aspergillus fumigates in 2[3.33]. Candida species in 2 [3.3%] and Mucor in 1 [1.6%] of patients. The patients were of all age groups but majority were between 21 and 30 years and the male to female ratio was equal. No patient had fungal infection elsewhere in the body. The patients were called for regular follow-up for three weeks. In 30 cases tincture mertheolate was applied as topical antifungal agent after cleaning the external auditory canal, in 17 patient's clotrimazole and in rest of the 13 patients miconazole was used. On 7[th] day, only 8 [13.3%] patients grew fungi in culture. They became symptom free on 14[th] day and no fungal material could be seen on otoscopy, direct microscopy or culture. Tincture mertheolate was found to be most effective in these patients

Effect of myoinositol on sporangiospore-yeast transformation of Mucor circinelloides Tieghem cultivated in synthetic broth.

Mucor species exhibit fungal dimorphism in controlled environments. In this work, we examined the effect of myoinositol supplementation on the growth and morphology of Mucor circinelloides. Using sporangiospores as inoculums, diverse morphologies were induced in synthetic broth incubated at pH 4.5, temp. 20 degrees C, ambient. The morphologies included thallic suptypes (holoblastic-, holothallic-, enterothallic conidia as well as vesicular conidial headgroups), which were determinate in growth, and proliferating yeast forms. Analysis of variance, p<0.05, showed that time had significant impact on growth. A separation of means, l.s.d. 14.34, p<0.05, indicated that myoinositol supplementation at 500 microM supported the least growth, but 2.0-3.0 mM levels had the higher values, and this was followed by the control, 300 microM, 1.0, 2.0, 5.0 mM supplementations. Although the predominant morphology, that is, terminal budding yeast cells was not quantitated, observation showed that it was more preponderant, had optimal size and cell shape became more regular at 2.0 mM myoinositol supplementation.

Acción antimicótica in vitro de hojas y ramitas de canelo (drimys winteri j. r. & g. forster) / In vitro antimycotic action of leaves and twigs of canelo (drimys winteri j. r. & g. forster)

La decocción y el extracto en acetato de etilo de hojas y ramitas trozadas de canelo (drimys winteri) inhibieron el desarrollo in vitro de candida albicans, aspergillus niger, a. fumigatus, rhizopus oryzae, mucor racemosus y m. hiemalis