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J. forensic med ; Fa yi xue za zhi;(6): 348-358, 2009.
Artigo em Chinês | WPRIM | ID: wpr-983500

RESUMO

OBJECTIVE@#To explore the correlation between signs similar to schizophrenia in mice after ketamine administration and the expressions of NRG1 and ErbB4 mRNA in order to explain the possible pathogenesis of schizophrenia.@*METHODS@#Fifty KM mice were randomly divided into 5 groups which were administered intraperitoneally with saline, clozapine and different dosages ketamine. The ketamine groups were administered intraperitoneally with low dosage (25 mg/kg), middle dosage (50 mg/kg) and high dosage (100 mg/kg) one time every day for 7 days. After administration of 100 mg/kg ketamine for 7 days, the clozapine group was introgastrically administered 20 mg/kg with clozapine one time every day for 7 days. The pathological changes of hippocampus neurons were observed by HE stain. The expressions of the NRG1 and ErbB4 mRNA in hippocampus were detected by reverse transcriptase polymerase chain reaction (RT-PCR).@*RESULTS@#In the group with high dosage of ketamine, the levels of NRG1 and ErbB4 mRNA were significantly lower than that of the group with saline.@*CONCLUSION@#Ketamine may induce signs similar to schizophrenia in KM mice. The mechanism may be involved in the reduction of NRG1 and ErbB4 mRNA expression.


Assuntos
Animais , Masculino , Camundongos , Clozapina/uso terapêutico , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Receptores ErbB/metabolismo , Hipocampo/patologia , Ketamina/efeitos adversos , Neuregulina-1/metabolismo , Neurônios/metabolismo , RNA Mensageiro/metabolismo , Distribuição Aleatória , Receptor ErbB-4 , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Esquizofrenia/genética
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