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1.
Rev. Asoc. Odontol. Argent ; 108(2): 46-51, mayo-ago. 2020. tab
Artigo em Espanhol | LILACS (Américas) | ID: biblio-1121108

RESUMO

Objetivos: Comparar ex vivo la eficacia del instrumento XP-endo Finisher y del sistema EndoActivator en la reducción/eliminación del biofilm microbiano en conductos radiculares infectados. Materiales y métodos: Se utilizaron 23 premolares inferiores humanos extraídos cuya longitud fue estandarizada en 17 mm. Todos los conductos se prepararon con el sistema WaveOne Gold Medium (#35.06). Los dientes se esterilizaron, se inocularon con Enterococcus faecalis y se separaron en dos grupos experimentales de 10 piezas cada uno. De los 3 dientes remanentes, 1 fue utilizado como control positivo y 2, como controles negativos. En el grupo 1, las soluciones irrigantes se agitaron con XP-endo Finisher. En el grupo 2, se utilizó EndoActivator. Se tomaron muestras antes de la contaminación, luego de esta y después de la agitación de los irrigantes mediante conos de papel estériles. La carga microbiana fue sembrada en agar sangre y los conos se cultivaron en caldo tripteína de soja. La remoción de la carga microbiana se determinó por la presencia o ausencia de turbiedad del medio. Las unidades formadoras de colonias (UFC) remanentes se cuantificaron y los resultados se categorizaron como R1 (≤10 UFC) o R2 (>10 UFC). Los datos fueron analizados mediante la prueba de Fisher. Resultados: No hubo diferencias significativas entre XP-endo Finisher y EndoActivator (P>0,05). El número de usos no influyó sobre la capacidad operativa de ambos instrumentos (AU)


Aim: To compare ex vivo the effectiveness of the XP-endo Finisher and the EndoActivator in biofilm reduction/ removal from infected root canals. Materials and methods: Twenty three extracted human single-rooted lower premolars were selected and standardised to 17 mm in length. All the canals were prepared with WaveOne Gold Medium reciprocating files (#35.06). The teeth were autoclaved and inoculated with Enterococcus faecalis. The infected teeth were then assigned to 2 experimental groups of 10 teeth each according to the final irrigation/agitation protocol. Of the three remaining teeth, one was used as a positive control, and the other two were used as negative controls. In Group 1 the irrigating solutions were agitated with XP-endo Finisher while in Group 2 the EndoActivator was used. All root canals were sampled before and after contamination, and again after irrigant agitation with sterile paper points. The microbial load was spread on blood agar plates and the paper points were cultured in sterile trypticase soy broth. The removal of the microbial load was determined by visual observation of the turbidity of the media and by quantification of the number of colony-forming units (UFC). The results were categorized as R1 (≤10 UFC) or R2 (>10 UFC). Data were analysed by the Fisher's exact test at P<0.05. Results: No significant differences was found between XP-endo Finisher and EndoActivator (P>0.05) regarding their effectiveness in the reduction/removal of the microbial biofilm. The number of uses of both instruments did not affect their operative performance (AU) Conclusion: XPF and EA were both equally effective for microbial biofilm reduction/removal from ex vivo infected root canals (AU)


Assuntos
Irrigantes do Canal Radicular/química , Equipamentos Odontológicos de Alta Rotação , Biofilmes , Instrumentos Odontológicos , Cavidade Pulpar/microbiologia , Técnicas In Vitro , Contagem de Colônia Microbiana/métodos , Eficácia , Análise Estatística , Enterococcus faecalis/isolamento & purificação , Meios de Cultura
2.
Vaccimonitor (La Habana, Print) ; 29(2)mayo.-ago. 2020. graf
Artigo em Espanhol | LILACS (Américas), CUMED | ID: biblio-1127511

RESUMO

Streptococcus pneumoniae es un patógeno oportunista que puede causar infecciones como otitis media, neumonía, sepsis y meningitis. Sin embargo, existen muchas limitaciones para el cultivo en zaranda de este microorganismo en los laboratorios de microbiología. Por esta razón, se realizó un estudio de la consistencia del cultivo en zaranda de Streptoccoccus pneumoniae 19A a escala de 40 L. Para esto se desarrolló previamente la curva de crecimiento patrón hasta las 5 h. Desde el pre-inóculo se inocularon 6 frascos de 100 mL, de los cuales se realizó la inoculación en botellones de 1 L y 5 L. En todos los casos, se determinó la pureza a partir de la tinción de Gram y el crecimiento bacteriano se monitoreó por el método de conteo de viables y la densidad óptica cada 1 h. Además, se evaluó el rendimiento del cultivo a partir de la cantidad de biomasa obtenida por peso húmedo. Cada proceso se llevó a cabo en condiciones iguales por triplicado. En los tres procesos se obtuvieron curvas de crecimiento similares, tanto por densidad óptica como por conteo de viables, alcanzando una viabilidad máxima de 109 UFC/mL en la última escala. Además, se obtuvieron rendimientos de biomasa de 11,62; 11,92 y 11,60 g/L, respectivamente. Estos resultados demuestran que la metodología utilizada ofrece una consistencia de este proceso, a pesar del alto volumen de cultivo en zaranda, lo cual no afectó la calidad de la biomasa, demostrado por la viabilidad final(AU)


Streptococcus pneumoniae is an opportunistic pathogen that can cause infections including otitis media, pneumonia, sepsis and meningitis. However, there are many limitations for the cultivation in shaker of this microorganism in microbiology laboratories. For this reason, a study of the consistency of the culture in shaker of Streptoccoccus pneumoniae 19A was carried out at a scale of 40 L. The pattern growth curve was made until 5 h, under our laboratory conditions and culture medium. From the pre-inoculum 6 bottles of 100 mL were inoculated, from which the scaling was accomplished to bottles of 1 L and 5 L. In all cases the purity was determined by Gram staining and the bacterial growth by viable counting method and the optical density were monitored every 1 h. In addition, the yield was evaluated from the determination of the amount of biomass obtained by wet weight. Each process has been made in equal conditions in triplicate. In the three processes, similar growth curves were obtained both by optical density and by viable counts, reaching a maximum viability of 109 CFU/mL on the last scale. In addition, biomass yields of 11.62, 11.92 and 11.60 g/L were obtained, respectively. These results demonstrate that the methodology used offers a high process consistency, despite the high volume of culture in rotational shaker, and did not affect the quality of the biomass, which could be demonstrated by the viable count(AU)


Assuntos
Infecções Pneumocócicas , Biomassa , Meios de Cultura
3.
Acta amaz ; 50(1): 61-67, jan. - mar. 2020.
Artigo em Inglês | LILACS (Américas) | ID: biblio-1118663

RESUMO

The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed. (AU)


Assuntos
Esporos , Ecossistema Amazônico , Meios de Cultura , Fungos
4.
São Paulo; SES/SP; 2020. 39 p.
Monografia em Português | LILACS (Américas), SES-SP, SESSP-ESPECIALIZACAOSESPROD, SES-SP | ID: biblio-1128731
5.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-816615

RESUMO

BACKGROUND: Acromegaly is a rare disease primarily caused by growth hormone (GH)-secreting pituitary adenomas, and its treatment is costly. Moreover, some patients are unresponsive to treatment. Hence, there are increasing efforts to develop new drugs with improved effectiveness for this disease. BIM23B065 is a novel chimeric molecule that acts on both somatostatin and dopamine receptors. This study aimed to investigate the effects of BIM23B065 compared with those of a somatostatin receptor analog and a dopamine agonist.METHODS: The effects of BIM23B065 on the proliferation, GH and insulin-like growth factor-1 (IGF-1) levels, and extracellular signal-regulated kinase (ERK) 1/2 and cyclic AMP response element binding (CREB) phosphorylation of GH3 cells were investigated with MTS assay, enzyme-linked immunosorbent assay, and Western blotting, respectively. The dosage and treatment duration of BIM23B065 were tested in animal models of GH-secreting pituitary adenoma. The effect of BIM23B065 (3 mg/kg/day) on changes in IGF-1 levels before and after treatment was further investigated.RESULTS: In vitro, BIM23B065 treatment decreased GH release in the culture media and downregulated ERK 1/2 and CREB phosphorylation to 22% and 26%, respectively. In vivo, IGF-1 expression decreased to 50 % after 4 weeks of treatment with BIM23B065 using an osmotic pump implant. Moreover, magnetic resonance imaging results showed that the tumor size decreased significantly following treatment with BIM23B065 for 4 weeks.CONCLUSION: The novel chimeric molecule was effective in decreasing IGF-1 and GH levels and may serve as an effective therapeutic agent for acromegaly.


Assuntos
Acromegalia , Western Blotting , Meios de Cultura , AMP Cíclico , Agonistas de Dopamina , Dopamina , Ensaio de Imunoadsorção Enzimática , Hormônio do Crescimento , Adenoma Hipofisário Secretor de Hormônio do Crescimento , Humanos , Técnicas In Vitro , Fator de Crescimento Insulin-Like I , Imagem por Ressonância Magnética , Modelos Animais , Fosforilação , Fosfotransferases , Neoplasias Hipofisárias , Doenças Raras , Receptores Dopaminérgicos , Receptores de Somatostatina , Elementos de Resposta , Somatostatina
6.
Rev. ADM ; 76(5): 261-266, sept.-oct. 2019. ilus, tab
Artigo em Espanhol | LILACS (Américas) | ID: biblio-1052989

RESUMO

Los profesionales de la salud están expuestos a una gran variedad de microorganismos desde esporas, bacterias, hongos, virus y protozoarios que pueden encontrarse en la sangre y/o saliva de los pacientes. Cualquiera de estos microorganismos puede causar una enfermedad infectocontagiosa a través de pinchazos y/o salpicaduras producidas por el aerosol durante la práctica dental. Objetivo: Determinar la presencia bacteriana en las piezas de alta velocidad utilizadas en la práctica clínica. Material y métodos: Es un estudio experimental, observacional y transversal en el que se evaluó la contaminación de 30 piezas de alta velocidad utilizadas en la práctica clínica. Previo al estudio se efectuó una estandarización obteniendo una kappa del 0.85. Se realizó una base de datos en el programa SPSS versión 22, con el que se llevó a cabo el análisis descriptivo para determinar medidas de tendencia central. Resultados: 73.3% de las muestras analizadas tuvieron crecimiento bacteriano, entre las bacterias que se encontraron resultó que 54.5% de ellas fueron bacterias Gram positivas y el resto Gram negativas. La bacteria con mayor presencia en la muestra fue el Bacillus en 45.5% seguida del Streptococcus en 27.3%, el restante 27.2% fue Staphylococcus, Coccus y Streptobacillus. Conclusiones: El uso correcto de las piezas de alta, así como su desinfección en la consulta dental es de suma importancia, ya que nos ayudan a evitar contaminaciones cruzadas y a prevenir que dentro del área de trabajo se formen focos de infección (AU)


Health professionals are exposed to a wide variety of microorganisms from spores, bacteria, fungi, viruses and protozoa that can be found in the blood and/or saliva of patients. Any of these microorganisms can cause an infectious disease through punctures and / or splashes produced by the aerosol during dental practice.1,2 Objective: To determine the bacterial presence in the high-speed pieces used in clinical practice. Material and methods: It are an experimental, observational and transversal study; where the contamination of 30 high-speed pieces used in clinical practice was evaluated. Prior to the study, a standardization was made obtaining a kappa of 0.85. A database was made in the program SPSS version 22, with which the descriptive analysis was carried out to determine measures of central tendency. Results: 73.3% of the analyzed samples showed bacterial growth, among the bacteria that were found, 54.5% of them were gram-positive bacteria and the rest were gram-negative. The bacterium with the highest presence in the sample was for Bacillus in 45.5% followed by Streptococcus in 27.3%, the remaining 27.2% was for Staphylococcus, Coccus and Streptobacillus. Conclusions: The correct use of the discharging parts, as well as their disinfection in the dental practice is of the utmost importance as they help us to avoid cross contamination and to prevent foci of infection from forming within the work area (AU)


Assuntos
Equipamentos Odontológicos de Alta Rotação/microbiologia , Contaminação Biológica , Controle de Infecções Dentárias/métodos , Contagem de Colônia Microbiana , Estudos Transversais , Análise Estatística , Meios de Cultura , Clínicas Odontológicas/normas , Estudo Observacional
8.
Prensa méd. argent ; 105(4): 192-196, jun 2019. tab, graf
Artigo em Inglês | LILACS (Américas), BINACIS | ID: biblio-1026848

RESUMO

It has been found that the antagonistic activity of lactic acid bacteria depends on the composition of the nutrient medium and the temperature of culturing. It has been shown that the best antimicrobial effect to mycobacteria is achieved by the cultivation of lactic acid bacteria on the MRS nutrient media and a combined nutrient medium with the use of lactulose or glucose as a source of carbon. The optimum temperature for culturing an association of lactic acid bacteria for achieving high antagonistic activity to mycobacteria is 300C, and the duration of cultivation is 24 hours.


Assuntos
Humanos , Tuberculose/etiologia , Ácido Láctico/antagonistas & inibidores , Probióticos/uso terapêutico , Meios de Cultura , Farmacorresistência Bacteriana/imunologia , Antibacterianos/uso terapêutico , Noxas
9.
Rev. ADM ; 76(2): 72-76, mar.-abr. 2019. tab, graf
Artigo em Espanhol | LILACS (Américas) | ID: biblio-1000403

RESUMO

Introducción: Los materiales para la obturación retrógrada son diversos. Actualmente, IRM y MTA son las alternativas clínicas más utilizadas, no obstante, es relativamente reciente la introducción de materiales a base de silicatos tricálcicos tal como Biodentine. Objetivo: Determinar la citotoxicidad de fibroblastos del ligamento periodontal humano expuestos a medios de cultivo condicionados con Biodentine, IRM y MTA. Material y métodos: 1 × 103 fibroblastos del ligamento periodontal humano fueron expuestos a medios DMEM/F12 condicionados con MTA, IRM y Biodentine en tres protocolos diferentes. Se realizó un ensayo de MTT para determinar la viabilidad celular a las cero, 24, 48, 72 horas, siete y 14 días. Se realizó una prueba ANOVA (p < 0.05). Resultados: En los tres protocolos con los diferentes medios de cultivo condicionados, la viabilidad de las células fue predominantemente proliferativa; sin embargo, las células expuestas a Biodentine mostraron una tendencia mayor que la MTA o la IRM. Conclusión: Las células expuestas a la Biodentine mostraron un comportamiento proliferativo a los 14 días de análisis. Se debe realizar más investigación a nivel in vivo y clínico para obtener más información sobre la conducta de estos materiales empleados para la obturación retrógrada (AU)


Introduction: The materials for retrograde filling are diverse. Currently, IRM and MTA are the most commonly used clinical alternatives, however, the introduction of materials based on tricalcium silicates such as Biodentine is relatively recent. Objective: To determine the cytotoxicity of human periodontal ligament fibroblasts exposed to culture media conditioned with Biodentine, IRM and MTA. Material and methods: 1 × 103 fibroblasts of the human periodontal ligament were exposed to DMEM/F12 media conditioned with MTA, IRM and Biodentine in 3 different protocols. An MTT assay was performed to determine cell viability at 0, 24, 48, 72 hours, seven and 14 days. An ANOVA test was performed (p < 0.05). Results: In the three protocols with the different conditioned culture media, the viability of the cells was predominantly proliferative, however, the cells exposed to Biodentine showed a higher tendency than the MTA or the IRM. Conclusion: The cells exposed to the Biodentine showed a proliferative behavior at 14 days of analysis. More research should be done at in vivo and clinical level to obtain more information about the behavior of these materials used for retrograde filling (AU)


Assuntos
Humanos , Materiais Restauradores do Canal Radicular/classificação , Materiais Restauradores do Canal Radicular/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Ligamento Periodontal , Obturação Retrógrada , Análise de Variância , Compostos de Cálcio , Compostos de Alumínio , Meios de Cultura , Fibroblastos
10.
Rev. ADM ; 76(2): 77-80, mar.-abr. 2019. ilus, tab
Artigo em Espanhol | LILACS (Américas) | ID: biblio-1005030

RESUMO

La OMS y la FDI han publicado que entre el 60 y 90% de los escolares padecen caries. En nuestro país, el Sistema de Vigilancia Epidemiológica de Patologías Orales (SIVEPAB) 2012, reporta un 85% de caries a nivel nacional en población pediátrica. Los agentes anticariogénicos como el diamino y el fluoruro de plata son un tratamiento alentador, este agente puede actuar como bactericida o bacteriostático en función de su concentración y su capacidad para inhibir el crecimiento de estreptococos del grupo viridans, y por ende, de la caries. Problema: ¿Cuál es la efectividad bactericida del diamino fluoruro de plata (Saforide®) a diferente concentración sobre la microbiota cariogénica de escolares? Objetivo: Determinar la eficacia bactericida del diamino fluoruro de plata (DFP) a diferentes concentraciones en el crecimiento bacteriano de Streptococcus mitis, S. mutans y S. salivarius en muestras de saliva y dentina en escolares. Material y métodos: Se llevó a cabo un estudio experimental con una variable independiente, el efecto bactericida del diamino fluoruro de plata y se tomó el halo de inhibición como la dependiente. Se utilizaron medidas descriptivas como prueba de comparación y análisis de varianza usando post-hoc Tukey≠ con una confianza del 95%, y análisis de datos exploratorios. Resultados: Se analizaron 100 muestras, de las cuales 48.3% correspondió a S. mutans, 41.4% a S. salivarius y 10.3% a S. mitis, se obtuvo una mayor zona de inhibición para las tres bacterias al 38% mostrando una diferencia estadísticamente significativa 12% (p < 0.05). También se observó un efecto bacteriostático al 12%, no así para el 38%, donde se encontró un efecto bactericida Conclusión: Nuestros resultados sugieren que al 38% de la concentración hay un claro efecto bactericida en el grupo de estreptococos viridans y el 12% no se recomienda para la detención de caries debido al efecto bacteriostático (AU)


WHO and FDI have ruled that 60-90% of schoolchildren are affected by caries. In our country, the System of Epidemiological Surveillance of Oral Pathologies (SIVEPAB) (SIVEPAB) 2012. Report a rate of 85% of caries nationally in pediatric population. Anticariogenic diamino agents such as silver fluoride are an encouraging decrease in treatment for these high rates of tooth decay in our country, this agent can act as bactericidal or bacteriostatic based on their concentration and their ability to inhibit endogenous metalloproteinase (MMP-2, 8, 9). Problem: What will be the bactericidal effectiveness of silver diamine fluoride different concentration on cariogenic Streptococci saliva samples taken from school and dentin? Objective: Determine the bactericidal effectiveness Silver diamine fluoride (SDF) to different concentration on bacterial growth of Streptococcus mitis, S. mutans, and S. salivarius in saliva samples and dentin in school. Material and methods: An experimental study was conducted as an independent variable the bactericidal effect of silver diamine fluoride was taken as dependent inhibition halo. Descriptive measures were used as a comparison test and analysis of variance using Post-hoc Tukey with 95% confidence, and exploratory data analysis. Results: One hundred samples, of which 48.3% corresponded to S. mutans, 41.4% to S. salivarius and 10.3% to S. mitis, were analyzed, we obtained a larger zone of inhibition for all three organisms at 38% showing a statistically significant difference from 12% (p < 0.05). It was also observed that the 12% sample bacteriostatic effect, not to the concentration of 38% was found a bactericidal effect. Conclusion: Our results suggest that 38% concentration has a bactericidal effect on Streptococcus viridans group and 12% showed not recommended for the arrest or detention of dentine caries bacteriostatic effect (AU)


Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Serviços de Odontologia Escolar , Streptococcus mutans/efeitos dos fármacos , Cariostáticos/uso terapêutico , Fluoretos Tópicos/uso terapêutico , Cárie Dentária/prevenção & controle , Saliva/microbiologia , Análise de Variância , Resultado do Tratamento , Compostos de Prata/uso terapêutico , Meios de Cultura , Dentina/microbiologia , México
11.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-761920

RESUMO

BACKGROUND: Mesenchymal stromal cells (MSCs) have potent immunomodulatory and neuroprotective properties, and have been tested in neurodegenerative diseases resulting in meaningful clinical improvements. Regulatory guidelines specify the need to perform preclinical studies prior any clinical trial, including biodistribution assays and tumourigenesis exclusion. We conducted a preclinical study of human bone marrow MSCs (hBM-MSCs) injected by intrathecal route in Non-Obese Diabetic Severe Combined Immunodeficiency mice, to explore cellular biodistribution and toxicity as a privileged administration method for cell therapy in Friedreich's Ataxia. METHODS: For this purpose, 3 × 10⁵ cells were injected by intrathecal route in 12 animals (experimental group) and the same volume of culture media in 6 animals (control group). Blood samples were collected at 24 h (n = 9) or 4 months (n = 9) to assess toxicity, and nine organs were harvested for histology and safety studies. Genomic DNA was isolated from all tissues, and mouse GAPDH and human β2M and β-actin genes were amplified by qPCR to analyze hBM-MSCs biodistribution. RESULTS: There were no deaths nor acute or chronic toxicity. Hematology, biochemistry and body weight were in the range of normal values in all groups. At 24 h hBM-MSCs were detected in 4/6 spinal cords and 1/6 hearts, and at 4 months in 3/6 hearts and 1/6 brains of transplanted mice. No tumours were found. CONCLUSION: This study demonstrated that intrathecal injection of hBM-MSCs is safe, non toxic and do not produce tumors. These results provide further evidence that hBM-MSCs might be used in a clinical trial in patients with FRDA.


Assuntos
Animais , Bioquímica , Peso Corporal , Medula Óssea , Encéfalo , Terapia Baseada em Transplante de Células e Tecidos , Meios de Cultura , DNA , Ataxia de Friedreich , Coração , Hematologia , Humanos , Injeções Espinhais , Células-Tronco Mesenquimais , Métodos , Camundongos , Doenças Neurodegenerativas , Neuroproteção , Valores de Referência , Imunodeficiência Combinada Severa , Medula Espinal
12.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-785643

RESUMO

OBJECTIVE: In vitro maturation (IVM) of immature oocytes can be useful for some infertile patients. In IVM programs, the rates of embryo formation and pregnancy are low. Therefore, it is essential to recognize the main factors involved in regulating oocyte maturation in vitro. The purpose of this study was to investigate the effects of growth differentiation factor 9 (GDF9) and cumulus cell (CC) supplementation in IVM medium on the rates of embryo formation and viability of human blastocysts.METHODS: A total of 80 germinal vesicle oocytes from stimulated cycles underwent an IVM program. The oocytes were divided into four groups, where group I consisted of IVM media only and served as the control, group II consisted of IVM+CCs, group III consisted of IVM+GDF9 (200 ng/mL), and group IV consisted of IVM+CCs+GDF9 (200 ng/mL). Intracytoplasmic sperm injection was performed on the IVM oocytes, and the cleavage embryos that were generated were vitrified. Following thawing, the embryos were cultured for 3 additional days, and the viability rates of the developed blastocysts were determined.RESULTS: The maturation rate of the oocytes did not differ significantly across the four groups. The fertilization rate in group II was significantly higher than that in the control group (76.5% vs. 46.2%). Embryo formation was significantly more frequent in all experimental groups than in the control group, while blastocyst formation did not show significant differences in the three experimental groups compared to the control. The mean viability rates in groups II, III, and IV were 58.16%, 55.91%, and 55.95%, respectively, versus 37.78% in the control group (p<0.05).CONCLUSION: Supplementation of IVM culture media with GDF9 and CCs enhanced the fertilization, embryo formation, and viability rates of blastocysts generated from vitrified cleavage embryos.


Assuntos
Blastocisto , Meios de Cultura , Células do Cúmulo , Estruturas Embrionárias , Fertilização , Fator 9 de Diferenciação de Crescimento , Humanos , Técnicas In Vitro , Oócitos , Gravidez , Injeções de Esperma Intracitoplásmicas
13.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-773132

RESUMO

In order to accelerate the breeding of the excellent seedlings of Polygonatum cyrtonema,tissue culture system of P. cyrtonema was established through the comprehensive regulation of key factors( leaf age,leaf location,basic media and plant growth regulators) and cytological basis of callus formation and differentiation was analyzed through paraffin section. The results showed that the 30-day-old leaf base explanton medium MS+6-BA 1. 50 mg·L~(-1)+2,4-D 0. 20 mg·L~(-1) had the highest induction rate( 80. 00%). The callus was initiated from cells on leaf base epidermis and near cortex,formed by the differentiation of middle vascular bundle cells. The optimal medium for adventitious bud differentiation was MS+ 6-BA 4. 00 mg·L~(-1)+ 2,4-D 0. 20 mg·L~(-1) with the differentiation rate of90. 33%,and the average number of buds was 5. 16. The adventitious buds had two origin types: exogenous and endogenous origin,formed by callus proximal cells and callus internal meristemoid. The adventitious bud proliferation medium was screened by orthogonal design,which determined the optimum combination was MS+ 6-BA 2. 00 mg·L~(-1)+NAA 0. 10 mg·L~(-1) and MS+ 6-BA 2. 00 mg·L~(-1)+NAA 0. 20 mg·L~(-1). The tubers with three leaves were cut and inoculated in the medium 1/2 MS+IBA 2. 00 mg·L~(-1),showing the highest rooting rate of 94. 00%. The rooting seedlings transplanted into the peat-vermiculite( 1 ∶ 1) matrix grew healthy and the survival rate was over 85. 00%. This research provided a novel solution for large-scale cultivation of P. cyrtonema seedling.


Assuntos
Meios de Cultura , Reguladores de Crescimento de Planta , Folhas de Planta , Biologia Celular , Polygonatum , Regeneração , Plântula , Técnicas de Cultura de Tecidos
14.
Chinese Journal of Biotechnology ; (12): 298-306, 2019.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-771376

RESUMO

The aim of the study was to obtain the secondary metabolites in the stem segment of noni and to establish genetic transformation system. The stem segments (no axillary buds) of noni were used as explants to induce the callus, and then to establish the cell suspension system. The factors affecting callus induction and cell suspension were studied. The results showed that the optimal culture medium for induction was MS with 1.0 mg/L 6-Benzylaminopurine (6-BA) and 0.1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and the optimum culture medium for suspension was MS with 1.0 mg/L 6-BA and 0.1 mg/L 2,4-D, 3% sucrose and the pH of 5.85, with the initial inoculation amount of 37.5 g/L, and the speed of 110 r/min and 25±2 °C applying darkness culture. The suspension cells grew well and showed the maximum growth rate. The growth curve of the suspension cells from the stem segment of noni was in "S-typed" trend, and it should be transformed to the fresh medium between 12 and 20 d. During the culture, the pH of the culture medium decreased and then slowly increased, and the optimum pH for the suspension cells culture of callus from noni's stem segments was 4.5-5.0. In this study, the stable cell suspension system of the stem segment of noni was successfully established.


Assuntos
Técnicas de Cultura de Células , Meios de Cultura , Morinda , Sacarose , Suspensões
15.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-776547

RESUMO

OBJECTIVE@#To investigate the mechanism of high glucose affecting the apoptosis of schwann cells through Nox4 NADPH oxidase.@*METHODS@#The schwann cells of newborn Wistar rats were cultured in vitro. The cultured cells were divided into four groups: control group, high-glucose group, NOX4 siRNA group and control siRNA group (n=10). The WST-1 method was used to detect the cell vitality, and the DCFH-DA method was used to detect the contents of intracellular reactive oxygen free radicals (ROS). Nox4 and Caspase3 mRNA expressions were detected by real-time fluorescence quantitative RT-PCR. Nox4 and Caspase3 protein expressions were determined by Western blot.@*RESULTS@#High glucose culture up-regulated Nox4 mRNA and protein expressions of schwann cells, decreased activity of schwann cells, increased intracellular ROS content, and promoted apoptosis by increasing Caspase3 mRNA and protein expressions. NOX4 siRNA blocked the accumulation of ROS in the high glucose cultured schwann cells, and reduced the damage of glucose on cell viability, by inhibiting NOX4 gene expression. NOX4 siRNA also reduced cell apoptosis by down-regulating Caspase3 mRNA and protein expressions.@*CONCLUSION@#Nox4 was involved in the hyperglycemic-induced apoptosis of schwann cells through ROS. The regulation of Nox4 expression or function might be a new way to treat diabetic peripheral neuropathy.


Assuntos
Animais , Apoptose , Caspase 3 , Metabolismo , Células Cultivadas , Meios de Cultura , Glucose , NADPH Oxidase 4 , Metabolismo , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio , Metabolismo , Células de Schwann , Biologia Celular , Metabolismo
16.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-816601

RESUMO

BACKGROUND: A major complication of peritoneal dialysis (PD) is peritonitis, and bacterial culture of PD effluent in a blood culture bottle is the preferred technique for diagnosis of peritonitis. In this study, we compared dialysate inoculation and culture using the BacT/AlerT® Fastidious Antimicrobial Neutralization Plus blood culture bottles (FAN Plus; bioMérieux, France) to the conventional centrifugation culture method.METHODS: A total of 170 PD effluents were simultaneously processed by the conventional centrifugation culture method and by culture using FAN Plus media with two different inoculation procedures: inoculation after centrifugation and direct bedside inoculation.RESULTS: Of the 52 cultures that were positive on at least one of the culture methods, 27 samples were positive on conventional centrifugation. However, 46 samples showed growth following inoculation into the FAN Plus media after centrifugation, and 47 samples were positive on the direct FAN Plus inoculation method. Using the case definition for PD peritonitis to classify samples, sensitivity of the conventional method was 50.0% (95% CI, 33.7–66.3%), whereas the sensitivity of the FAN Plus media was 78.9% (95% CI, 62.2–89.9%) by inoculation after centrifugation and 86.8% (95% CI, 71.1–95.1%) by direct inoculation. Use of both inoculation methods with FAN Plus media resulted in 92.1% sensitivity (95% CI, 89.2–99.9%).CONCLUSION: Culture using FAN Plus media demonstrated a superior bacterial recovery rate to the conventional centrifugation culture method. A combination of the two inoculation methods with FAN Plus media is recommended for the best diagnostic yield, while direct inoculation alone can be useful due to its simplicity and cost-effectiveness.


Assuntos
Centrifugação , Meios de Cultura , Diagnóstico , Métodos , Diálise Peritoneal , Peritonite
17.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-787351

RESUMO

The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro MTA® (RM), EZ-Seal™ (EZ) and ENDOCEM Zr® (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ (p = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.


Assuntos
Cálcio , Meios de Cultura , Citometria de Fluxo , Humanos , Fenótipo , Células-Tronco , Dente Decíduo
18.
Acta odontol. latinoam ; 32(1): 36-43, 2019. tab
Artigo em Inglês | LILACS (Américas) | ID: biblio-1015020

RESUMO

The aim of this study was to evaluate the deproteinization of primary enamel by analyzing etching pattern types, with and without the application of 5% NaOCl before acid etching with 37% H3PO4. Fifteen extracted human primary molars were randomly selected for the present in vitro study; 1mm x 1mm blocks were prepared and divided into two groups (n = 21). These groups were treated as follows: Group AAcid Etching with 37% H3PO4 gel for 15 s; Group B5% NaOCl for 60 s + Acid Etching with 37% H3PO4for 15 s. The specimens were prepared for scanning electron microscopy analysis. The images were evaluated for quality types I and II etching of the enamel surface using ImageJ software. Datasets were checked for normality by KolgomorvSmirnov test and the nonparametric unpaired MannWhitney test was applied. The mean surface area of type I and II etching pattern values was 1922.314 µm2for Group A and 3840.473 µm2Group B. We conclude that deproteinization with 5% NaOCl prior to acid etching can be used to increase the area of adhesion and the quality of the etching pattern (AU)


El objetivo del estudio fue evaluar la desproteinización del esmalte primario a través de los tipos de patrones de grabado, con y sin NaOCl 5% utilizado antes del grabado ácido con H3PO4 37%. Quince dientes primarios humanos extraídos se seleccionaron al azar para el presente estudio in vitro, se prepararon bloques de 1mm x 1 mm y se dividieron en dos grupos (n = 21). Estos grupos se trataron de la siguiente manera: Grupo A: Grabado ácido con H3PO4 37% en gel durante 15 segundos; Grupo B: NaOCl 5% durante 60 segundos + Grabado ácido con H3PO4 37% durante 15 segundos. Las muestras se prepararon para el análisis de microscopía electrónica de barrido. Las imágenes obtenidas se evaluaron principalmente por la calidad de los grabados tipo I y II de la superficie del esmalte primario, utilizando el software Image J. Los datos se analizaron en cuanto a su normalidad mediante la prueba de KolgomorvSmirnov, se utilizó pruebas no paramétricas: Prueba de MannWhitney no pareada. Como resultado, se encontró que el área de superficie media de los valores de patrón de grabado de tipo I y II para el Grupo A era 1922,314 µm2 y el Grupo B era 3840,473 µm2. Finalmente, llegamos a la conclusión de que se puede usar la desproteinización con NaOCl 5% antes del grabado ácido para aumentar el área de adhesión y la calidad del patrón de grabado (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Periodontite/microbiologia , Meios de Cultura , Contagem de Colônia Microbiana/métodos , Estudos Transversais , Análise Estatística , República Dominicana
19.
Arq. odontol ; 55: 1-12, jan.-dez. 2019. ilus, tab
Artigo em Português | LILACS (Américas), BBO | ID: biblio-1052824

RESUMO

Objetivo: Avaliar as alterações químicas presentes na superfície metálica de limas endodônticas fraturadas em canais radiculares, in vitro, após a inoculação intrarradicular de culturas de BRS de três cepas microbianas, Desulfovibrio desulfuricans (uma cepa oral e outra ambiental) e Desulfovibrio fairfieldensis. Métodos: foram analisadas 5 limas kerr #90, sendo uma Lima Kerr nova, sem tratamento, e as outras 4 limas fraturadas dentro de canais radiculares in vitro, com posterior inoculação de Desulfovibrio desulfuricans, cepa oral e ambiental, e Desulfovibrio fairfieldensis e um grupo controle sem inoculação bacteriana, por 477 dias. Os grupos foram analisados no modo EDS (Espectrometria de Energia Dispersiva de Raios-x) do microscópio eletrônico de varredura (FEI-Inspect-S50). Resultados:A presença do S, Cl e O foram relacionados ao processo biocorrosivo, assim como a redução dos elementos de liga nesta área. Conclusão:As análises no modo EDS demonstraram biocorrosão ao longo da superfície metálica das limas quando empregado o biofármaco BACCOR, nas três diferentes cepas empregadas, indicada pela redução dos elementos formadores da liga metálica, Fe, Ni e Cr, com a associação da presença de elementos indicadores de biocorrosão como O, Cl e S. (AU)


Aim:To evaluate the chemical alterations present on the metallic surface of root canal fractured endodontic files in vitro after the intraradicular inoculation of BRS cultures of three microbial strains, Desulfovibrio desulfuricans (one oral and one environmental strain), and Desulfovibrio fairfieldensis. Methods: Five kerr #90 files were analyzed, one new untreated Kerr file and the other 4 files fractured within root canals in vitro, with a subsequent inoculation of Desulfovibrio desulfuricans (oral and environmental strains), and Desulfovibrio fairfieldensis, as well as a control group without bacterial inoculation for 477 days. The groups were analyzed using the scanning electron microscope (FEI-Inspect-S50) EDS (X-ray Dispersive Energy Spectrometry) mode. Results:The presence of S, Cl, and O were related to the biocorrosive process, as well as the reduction of alloying elements in this area. Conclusion: The EDS mode analysis showed biocorrosion along the metallic surface of the files when the BACCOR biopharmaceutical was used in the three different strains employed in this study, indicated by the reduction of the alloying elements ­ Fe, Ni, and Cr ­ with the association of the presence of indicator elements of biocorrosion, such as O, Cl, and S. (AU)


Assuntos
Produtos Biológicos , Corrosão , Meios de Cultura , Ligas Dentárias , Instrumentos Odontológicos , Desulfovibrio desulfuricans , Cavidade Pulpar , Desulfovibrio , Técnicas In Vitro , Endodontia
20.
Rev. Fundac. Juan Jose Carraro ; 23(43): 19-25, 2019.
Artigo em Espanhol | LILACS (Américas) | ID: biblio-1050126

RESUMO

Las enfermedades del periodonto tienen una etiopatogenia compleja y puede considerarse multifactorial. El factor etiológico esencial en la patología inflamatoria periodontal es la biopelícula dental y cuando el desequilibrio entre el huésped y los microorganismos cambia la complejidad de la flora. Ciertas bacterias como Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii, Fusobacterium nucleatum, Tannerrella forsythia, Campylobacter rectus, Eikenella corrodens y Treponema spp., han sido comúnmente relacionadas con la periodontitis crónica y son consideradas como indicadores de riesgo para la progresión de dicha enfermedad. El objetivo de este trabajo fue establecer la prevalencia de Prevotella spp y Porphyromona spp en los distintos estadios de periodontitis crónicas. Material y métodos: Se estudiaron 48 pacientes sistémicamente saludables con diagnóstico de periodontitis crónica. Se completó el consentimiento informado, se realizó historia clínica y examen periodontal. El estado periodontal se clasificó en distintos grados de severidad: leve, moderada y severa. Se tomaron muestras de dos sitios con mayor profundidad de sondaje con conos de papel absorbente estériles y se transportaron en un medio prerreducido. Para el aislamiento de Prevotella spp se utilizó agar Brucella más sangre ovina al 5%, hemina, vitamina K al que se agregaron vancomicina y kanamicina; Porphyromonas sp se aisló en el mismo medio con el agregado de bacitracina y colistina. Se sembraron 10 µl de muestra entera y las placas fueron incubadas en jarras de anaerobiosis por 5 a 7 días a 37ºC. Resultados: los distintos grados de periodontitis correspondieron a un 17% periodontits leve, 57% moderada y 26% severa. En el total de pacientes se determinó la presencia de Prevotella spp en el 54% de los casos y un 12,5% de Porphyromona spp. Conclusión: De los pacientes estudiados con periodontits crónica, un 52% correspondió al sexo masculino, un 57% de los casos correspondieron a periodontitis moderada. Se aisló Prevotella sp en todos los estadios de periodontitis crónica y Porphyromonas sp sólo en periodontitis severas (AU)


Periodontal diseases have a complex etiopathogenesis and can be considered multifactorial. The essential etiological factor in periodontal inflammatory pathology is the dental biofilm and when the imbalance between the host and the microorganisms changes the complexity of the flora. Certain bacteria such as Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii, Fusobacterium nucleatum, Tannerrella forsythia, Campylobacter rectus, Eikenella corrodens and Treponema spp., Have been commonly related to chronic periodontitis and are considered as risk indicators for the progression of said disease. The objective of this work was to establish the prevalence of Prevotella spp and Porphyromonas spp in the different stages of chronic periodontitis. Forty eight systemically healthy patients diagnosed with chronic periodontitis were studied. Informed consent was completed, a medical history and periodontal examination was carried out. The periodontal state was classified into different degrees of severity: mild, moderate and severe. Samples were taken from two sites with greater depth of probing with sterile absorbent paper cones and transported in a prereduced medium. For the isolation of Prevotella spp, Brucella agar plus 5% sheep blood, hemin, vitamin K to which vancomycin and kanamycin were added. For Porphyromonas spp, the same medium was used and bacitracin and colistin were added. 10 �l of the whole sample was seeded and the plates were incubated in anaerobic jars for 5 to 7 days at 37 ° C. Different degrees of periodontitis corresponded to 17% mild periodontitis, 57% moderate and 26% severe. In the total number of patients, the presence of Prevotella spp was determined in 54% of the cases and 12.5% of Porphyromona spp. Of the patients studied with chronic periodontitis, 52% corresponded to the male sex, 57% of the cases corresponded to moderate periodontitis. Prevotella spp was isolated in all stages of chronic periodontitis and Porphyromonas sp only in severe periodontitis (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Infecções por Bacteroidaceae/epidemiologia , Porphyromonas/isolamento & purificação , Prevotella/isolamento & purificação , Periodontite Crônica/microbiologia , Contagem de Colônia Microbiana , Meios de Cultura , Distribuição por Idade e Sexo
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