Реферат
Salmonella infection in bird species in Jamaica was studied. This revealed that very low prevalence of salmonellosis was found (0.32 %). Salmonella Yeerongpilly (newly reported in the country) was isolated from a bird collected at a bird aviary. This study showed that there was the presence of this Salmonella serovar in a Chinese owl (Columba livia domestica) in Jamaica. There were not published reports from Caribbean Islands of the presence of this serovar. Salmonella Yeerongpilly belongs to serogroup E1 and by molecular serotyping random amplification of polymorphic DNA (RAPD) fingerprinting belongs to A20, B17 and C21. This strain was isolated in Queensland Australia in the 1960s before the successful Salmonella eradication campaign. This study suggests that a larger investigation in pet birds as Salmonella carriers should be carried out in Jamaica. Mandatory screening or quarantine of birds entering the country should be institutionalized.
Тема - темы
Animals , Bird Diseases/epidemiology , Bird Diseases/microbiology , China , Columbidae/classification , Columbidae/microbiology , Jamaica , Salmonella/classification , Salmonella/epidemiology , Salmonella Infections/epidemiologyРеферат
The aim of this study was to vaccinate layer hen chickens against Salmonella infection. Two vaccines were assessed for efficacy and safety: a DNA vaccine containing Salmonella genomic DNA encapsulated in a liposome as a vector and a live attenuated Salmonella vaccine comprising 5 attenuated Salmonella serovars that were attenuated using indigenous plant extracts such as garlic and onion. The results showed that both vaccines had a high protection capacity, preventing Salmonella infection after challenge with a wild type of SalmonellaTyphimurium. Hyper-immune eggs inhibited the growth of Salmonella spp in vitro in immunized chickens. ELISA demonstrated the specific antibody production to LPS of S. Typhimurium. Post-mortem studies confirmed the presence of salmonellosis in the control group but not in immunized chickens with either vaccine. This study shows that Poultry salmonellosis can be prevented by the use of prophylactic DNA or live-attenuated vaccines (LAV).
Реферат
Aim: This study was undertaken to determine the prevalence of Salmonella spp contamination in the Jamaican poultry industry and its environments. Materials and Methods: A total of 45 farms across 6 Jamaican parishes were selected for this study. A total of 6693 specimens from animals and the environment were investigated for the presence of Salmonella spp. All specimens were placed in an igloo with ice packs and transported to the laboratory for analysis. Bacteriological media obtained from Difco Laboratories Detroit MI U.S.A were used for the isolation and identification of Salmonella spp. Salmonella serological typing was performed to determine the Salmonella serovar by standard procedures. Results: This study revealed a low prevalence of Salmonella contamination/infection in both small and large entities in the poultry industry in Jamaica. The overall prevalence was 1 % (79 positive out of 6693 specimens). However, a higher prevalence of Salmonella was observed in the case of those operations which practiced “organic” poultry farming. It was shown that two Salmonella serovars including Augustenborg and Kentucky, identified during the study, are newly reported serovars in Jamaica. The sources of Salmonella infection varied from poultry itself to other species, such as rodents, pigs and insects. Improper disposal of broken eggs, wet bedding and other fomites contributed to Salmonella contamination. Conclusions: The results of the study indicate possibility of salmonellosis (zoonosis) in Jamaica, although the prevalence of Salmonella spp was low, and the need for improved quality of the food industry, animal care and human health to prevent salmonellosis.