Реферат
Objective:To investigate the effect and mechanism of longikaurin A (LK-A) on glioma proliferation.Methods:Resuscitated frozen human glioma cell lines U87 and SNB19 were in vitro sub-cultured; CCK-8 assay was used to detect the cell activity changes 24 and 48 h after 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 6.0, 8.0 μmol/L LK-A. Edu proliferation assay was used to detect the changes in number of Edu positive cells after 0, 1, 2 and 3 μmol/L LK-A, and cell clonal formation assay was used to detect the changes in number of cell colony formation after 0, 0.1, 0.2 and 0.3 μmol/L LK-A. Flow cytometry was used to detect the changes in cell cycle proportion after 0, 1, 2 and 3 μmol/L LK-A. Expression changes of proliferation-related proteins (Ki-67 and c-Myc) and cell cycle-related proteins (Cyclin B1, Cyclin D1, cyclin dependent kinase 1 [CDKl]) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway-related proteins were detected by Western blotting after 0, 1, 2, and 3 μmol/L LK-A. Results:U87 and SNB19 cell viabilities decreased gradually after being treated with 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 6.0, and 8.0 μmol/L LK-A for 24 and 48 h compared with those with 0 μmol/L LK-A, with significant differences ( P<0.05); cell viability was dose-dependent. The number of Edu positive cells in U87 and SNB19 after being treated with 2, and 3 μmol/L LK-A was significantly decreased compared with that with 0 μmol/L LK-A ( P<0.05). The colony formation number of U87 and SNB19 cells after being treated with 0.1, 0.2 and 0.3 μmol/L LK-A decreased significantly compared with that with 0 μmol/L LK-A ( P<0.05). The proportion of U87 and SNB19 cells at G2/M phase after being treated with 2 and 3 μmol/L LK-A were increased significantly compared with that with 0 μmol/L LK-A ( P<0.05). The Ki-67, c-Myc, Cyclin B1, CDK1, p-PI3K and p-AKT expressions were significantly decreased, while Cyclin D1 expression was significantly increased in U87 and SNB19 cells after being treated with 2 and 3 μmol/L LK-A compared with those with 0 μmol/L LK-A ( P<0.05). Conclusion:LK-A can inhibit the glioma proliferation and arrest glioma at G2/M phase, with dose-dependent manner; the mechanism is related to inhibition of PI3K/AKT signaling pathway by LK-A.
Реферат
Objective:To explore the effects of hematoma removal by neuroendoscopy or craniotomy on thalamic hemorrhage.Methods:Eighty-three patients with thalamic hemorrhage, admitted to our hospital from September 2016 to March 2019, were chosen in our study; 44 patients accepted hematoma removal by neuroendoscopy and 39 patients accepted hematoma removal by craniotomy under microscope. These patients were divided into severe type ( n=55) and mild type ( n=28) according to Glasgow coma scale scores at admission. The efficacy and safety of the two procedures were compared. Patients were followed up until one year after onset, and their recovery was assessed by Glasgow outcome scale (GOS). Results:As compared with those in the craniotomy group, patients in the neuroendoscopy group had significantly small volume of intraoperative blood loss ([284.90±31.74] mL vs. [45.70±6.94] mL), significantly shorter hospital stays ([18.40±2.75] d vs. [14.70±2.13] d), and statistically lower incidence of epilepsy (15.4% vs. 2.3%, P<0.05). In critical survived patients, the GOS scores in the endoscopic group were 4.99±0.48 until one year after onset, which were significantly higher than those in the craniotomy group (2.64±0.41, P<0.05). Conclusion:As compared with hematoma removal by craniotomy, that by neuroendoscopy has the advantages of minimal invasion, good visual field, and high efficiency in the treatment of thalamic hemorrhage.
Реферат
BACKGROUND: It has been confirmed that a polymer scaffold with hydroxyapatite (HA) has good biocompatibility. Chitosan that is combined with other materials, such as HA, hyaluronic acid, alginate, and potential growth factors, can be applied in tissue engineering field. Meanwhile, numerous studies have confirmed that bone morphogenetic protein-2 (BMP-2)can promote the growth of osteoblasts and induce osteogenesis in vitro and in vivo.So,can we prepare a new tissue-engineered scaffold with these four kinds of materials? OBJECTIVE: To prepare a novel BMP-2-loaded tissue-engineered bone scaffold using poly(lactic-co-glycolic acid) (PLGA), HA and different concentrations of chitosan, and to observe the scaffold structure, hydrophilicity, and adherence to osteoblasts as well as the optimal modification concentration of chitosan. METHODS: (1) A tissue-engineered scaffold containing PLGA, HA and BMP-2 was prepared using the solid-liquid phase separation and modified by chitosan (0.25%, 0.5% and 1%). Additionally, PLGA/HA and PLGA/HA/BMP-2 scaffolds were prepared as controls. Scaffold structure was observed under a scanning electron microscope. The hydrophilicity of each scaffold and BMP-2 release of the PLGA/HA/BMP-2/chitosan scaffold were examined. (2) Pre-osteoblastic suspensions were seeded onto each scaffold. Cell adhesion and proliferation were detected using cell counting kit-8 at 1, 4, 7 days of cell culture. Fluorescein diacetate was used for a vital staining of cells at 7 days of cell culture. Alkaline phosphatase activity was detected at 4, 7 and 14 days of cell culture. RESULTS AND CONCLUSION: (1) All the scaffolds were white beaker-shaped and had porous structure with a pore size of about 100 μm, and interconnected pores were observed under the scanning electron microscope. (2) The scaffold hydrophilicity was increased with the increasing concentration of chitosan. (3) BMP-2 cumulative release amount was 44% for the PLGA/HA/BMP-2, 34% for PLGA/HA/BMP-2/0.25% chitosan, 27% for PLGA/HA/BMP-2/0.5% chitosan, and 26% for PLGA/HA/BMP-2/1% chitosan, indicating that chitosan can effectively slow the release of BMP-2. (4) Cell viability of pre-osteoblasts seeded onto the PLGA/HA/BMP-2/0.25% chitosan scaffold was highest at 7 days of cell culture. Higher cell viability of pre-osteoblasts seeded onto the PLGA/HA/BMP-2/chitosan (0.5%, 1%) scaffolds was also observed compared with two control scaffolds. After fluorescein diacetate staining, living cells with green fluorescence were evenly distributed on the scaffolds under the confocal laser microscope. (5) The alkaline phosphatase activity in cells seeded onto different scaffolds was ranked as follows: the PLGA/HA/BMP-2/0.25% chitosan scaffold > the PLGA/HA/BMP-2/0.5% chitosan scaffold > the PLGA/HA/BMP-2/1% chitosan scaffold > the PLGA/HA/BMP-2 scaffold > the PLGA/HA scaffold (P < 0.05). Taken together, the PLGA/HA/BMP-2/chitosan scaffold is suitable to release bioactive BMP-2 for stimulating cell adhesion, differentiation and proliferation, which is designed to optimize the tissue-engineered bone scaffold in bone tissue engineering strategies. And moreover, the optimal modification concentration of chitosan is 0.25%.
Реферат
Objective To analyze the surgical technique and success rate of endoscopic third ventriculostomy (ETV) in pediatric obstructive hydrocephalus.Methods A retrospective analysis of clinical data and treatment efficacy of 13 patients with pediatric obstructive hydrocephalus,admitted to and undergone ETV in our hospital fiom July 2008 to July 2014,was performed.All patients were confirmed by CT and MR imaging.Results Follow-up information for all patients was obtained every 6 months by telephone after operation.Re-check was performed in all patients by CT or MR imaging.Ten had signs of hydrocephalus greatly relieved or disappeared;3 were invalid,and ventriculoperitoneal shunt was then performed 3-12 months after ETV.Postoperative fever occurred in 2 children and epilepsy in one.No complications,such as cerebrospinal fluid leakage,bleeding,infection or nerve injury,were noted.Conclusion ETV in treatment of children with obstructive hydrocephalus is safe and effective,enjoying few complications.
Реферат
Objective To investigate the role of signal transducer and activator of transcription 3 (STAT3) knockdown in regulating the invasion and apoptosis of glioma cells.Methods Liposome-mediated STAT3 antisense oligonucleotide was transfected into the U251 glioma cells;nonsense sequence group and blank control group were also established.The effect of STAT3 antisense oligonucleotide on the growth of U251 glioma cells was examined by MTT assay; the cell cycle and apoptosis were evaluated by flow cytometry; the cell migration was determined by Transwell invasion assay.Western blotting was employed to explore the protein expressions of STAT3 and pSTAT3,urokinase-type plasminogen activator receptor (uPAR),Bax and Bcl-2 in glioma cells.Results As compared with the nonsense sequence group and blank control group,liposome-mediated STAT3 antisense oligonucleotide group had lower survival rate,inhibited cell proliferation and cells being arrested at G0/G1 phases; Transwell invasion assay indicated that STAT3 antisense oligonucleotide suppressed the invasion and promoted the apoptosis of U251 cells.The STAT3,pSTAT3,uPAR and Bcl-2 expression levels in the iposome-mediated STAT3 antisense oligonucleotide group were signficantly decreased as compared with those in the nonsense sequence group and blank control group,while Bax level was obviously elevated (P＜0.05).Conclusion STAT3 antisense oligonucleotide can inhibit the invasion and promote the apoptosis of U251 cells by regulating its downstream gene expression; STAT3 can be used as an effective target for glioma gene therapy.
Реферат
Objective To explore the effect ofmiR-137 on proliferation and apoptosis of U87 glioma cells.Methods The miR-137 mimics were transfected to human glioma cell line U87 as miR-137 transfection group; blank control group and negative control group were also employed in our study.The expression ofmiR-137 was identified by real-time polymerase chain reaction (PCR) after transfection.Methylthiazol tetrazoliu (MTT) assay,flow cytometry-Annexin V/PI assay and fluorescence microscope were employed to detect the cell proliferation and apoptosis.MiR-137 and its relative targeting protein expressions were detected by Westem blotting.Results MTT assay showed that the relative cell survival rates in the blank control group,negative control group and miR-137 transfection group were (105.16±8.57)％,(98.57±8.21)％ and (45.84±6.93)％,with significant differences (F=82.157,P=0.000).Annexin V/PI assay showed that the cell apoptosis rates in the blank control group,negative control group and miR-137 transfection group were (4.3±0.63)％,(4.7±0.77)％ and (16.6±1.98)％,with significant differences (F=63.837,P=0.000); and apoptotic body was detected by fluorescence microscope.Moreover,CDC42,pERK1/2,AKT and pAKT protein expression levels were inhibited after transfected by miR-137 mimics.Conclusion MiR-137 inhibits proliferation and induces apoptosis of U87 glioma cells,suggesting that miR-137 could be tumor suppressor gene in glioma.
Реферат
Background Trachoma is a common disease in ophthalmology,but few reports about the pathogenetic genotype are reported.Objective This study was to investigate the genotypes of Chlamydia trachomatis.Methods Conjunctival specimens were collected in 16 patients with trachoma in Beijing Tongren Eye Center from January,2003 and August,2006.Variable sequence 4 (VS4) of ompl fragment was amplified by nested PCR(n-PCR) using specific primer of Chlamydia trachomatis,and then the PCR products were sequenced.The DNA sequences were analyzed by software Clustal X and MEGA2,and the genetic characteristics were compared with the known sequences of GenBank.Results The PCR product fragment of MOMP gene of trachoma was 277 bp.Three types of Chlamydia trachomatis strains were idcntified in the 16 specimens,including B-genotype in 9 strains (56.3％),C-genotype in 4 strains(25.0％) and D-genotype in 3 strains(18.7％).High homology was seen in the gene sequences of Chlamydia trachomatis strains between the B-genotype or C-genotype strains and the same genotypes of GenBank or those from some districts of China.However,some differences were exhibited among the Dgenotype strains.Conclusions Identification of genotype of Chlamydia trachomatis has differential effect on trachoma and inclusion conjunctivitis.
Реферат
<p><b>OBJECTIVE</b>To study the expression of RAS protein in human glioma tissues and its influence on tumor growth.</p><p><b>METHODS</b>RAS protein expression in glioma tissues was determined by immunohistochemical (IHC) staining. Subsequently, MTT cell proliferation assay, flow cytometry and Western blotting were used to assay U251 cells with reduced RAS expression.</p><p><b>RESULTS</b>The expression of RAS in glioma was increased and strongly correlated with pathological grade. Downregulation of RAS resulted in glioma cells growth suppression and increased apoptosis.</p><p><b>CONCLUSION</b>The expression level of RAS protein in human glioma was increased. Downregulation of RAS can inhibit glioblastoma cell growth through the RAS signal pathway.</p>
Тема - темы
Humans , Brain Neoplasms , Genetics , Metabolism , Pathology , Cell Growth Processes , Genetics , Cell Line, Tumor , Down-Regulation , Glioma , Genetics , Pathology , Immunohistochemistry , ras Proteins , GeneticsРеферат
The aqueous phase oxidation of gaseous elemental mercury (Hg(0)) by potassium persulfate (KPS) catalyzed by Ag(+) was investigated using a glass bubble column reactor. Concentration of gaseous mercury and potassium persulfate were measured by cold vapor atom absorption (CVAA) and ion chromatograph (IC), respectively. The effects of pH value, concentration of potassium persulfate and silver nitrate (SN), temperature, Hg(0) concentration in the reactor inlet and tertiary butanol (TBA), free radical scavenger, on the removal efficiency of Hg(0) were studied. The results showed that the removal efficiency of Hg(0) increased with increasing concentration of potassium persulfate and silver nitrate, while temperature and TBA were negatively effective. Furthermore, the removal efficiency of Hg(0) was much better in neutral solution than in both acidic and alkaline solution. But the influence of pH was almost eliminated by adding AgNO(3). High Hg(0) concentration has positive effect. The possible reaction mechanism of gaseous mercury was also discussed.