A novel triterpenoid saponin from Prunella vulgaris / 药学学报

To study the constituents of the Prunella vulgaris L, the constituents were isolated by various column chromatography and the structures were identified on the basis of chemical and spectral analysis. One saponin compound (I) and one flavone glycoside compound (II) were obtained from Prunella vulgaris L. Their structures were elucidated as 16-oxo-17-demethyl-3beta,24-dihydroxylolean-12-en-3-O-beta-D-glucuronoside (I), and acacetin-7-O-beta-D-glucopyranoside (II). Compound I is a novel triterpenoid saponin and named as prunelloside A. Compound II was obtained for the first time from the Prunella genus.

Chemical constituents from Spatholobus sinensis / 药学学报

Spatholobus sinensis is a plant of the Spatholobus genus (Leguminosae family). Its caulis are used as "ji-xue-teng" regionally. However, to our knowledge, no phytochemical investigation on S. sinensis has been reported to date. In this study, eight compounds were isolated from the ethanol extract of the caulis of S. sinensis, by solvents extraction and column chromatography methods. By analysis of their physic-chemical constants and spectral data, the structures of 8 compounds were identified as spatholosineside A (1), 2',4',5,7-tetrahydroxyisoflavone (2), isoliquiritigenin (3), lupinalbin A (4), coumestrol (5), naringenin (6), protocatechuic acid (7), leonuriside A (8). Compound 1 is a new compound.

Isolation and structure identification of chemical constituents from Saposhnikovia divaricata (Turcz.) Schischk / 药学学报

Fourteen compounds were isolated from the ethanol extraction of Saposhnikovia divaricata (Turcz.) Schischk using column chromatographic methods after enrichment by macroporous adsorptive resins. They were identified as fangfengalpyrimidine (1), clemiscosin A (2), 5-hydroxy-8-methoxypsoralen (3), sec-O-glucosylhamaudol (4), hamaudol (5), nodakenetin (6), prim-O-glucosylcimifugin (7), cimifugin (8), 4'-O-beta-D-glucosyl-5-O-methylvisamminol (9), 5-O-methylvisamminol (10), marmesin (11), adenosine (12), daucosterol (13) and beta-sitosterol (14) by physico-chemical properties and spectral data. Compound 1 is a new compound. Compounds 2 and 3 were isolated from umbelliferae plants and Saposhnikovia divaricata (Turcz.) Schischk for the first time respectively.

Studies on lignan glycosides from the roots of Bupleurum scorzonerifolium / 药学学报

<p><b>AIM</b>To study the lignan glycosides from the roots of Bupleurum scorzonerifolium. Chromatography methods was used to isolate compounds and chemical and spectral methods were used to identify the structures of isolated compounds.</p><p><b>RESULTS</b>Two lignan glycosides were isolated from the roots of B. scorzonerifolium and their structures were identified as 2, 3-E-2,3-dihydro-2-(3'-methoxy-4'-O-beta-D-glucopyranosyl-phenyl)-3-hydroxymethyl-5-(3"-hydroxypropenyl )-7-methoxy-1-benzo [b] furan (1) and 2, 3-E-2, 3-dihydro-2-( 3'-methoxy-4'-hydroxy-phenyl)-3-hydroxymethyl-5-( 3"-hydroxypropenyl)-7-O-beta-D-glucopyranosyl-1-benzo[b]furan (2), both of them which were the mixture of (+)2S,3R- and (-)2R,3S-type diastereoisomer and in both of them the amount of (+)2S,3R-type was a little more than that of the (-)2R,3S-type.</p><p><b>CONCLUSION</b>Compounds 1 and 2 were isolated from B. scorzonerifolium for the first time. (+)2S, 3R-2, 3-Dihydro-2-(3'-methoxy-4'-hydroxy-phenyl)-3-hydroxymethyl-5-(3"-hydroxypropenyl)-7-O-beta-D-glucopyranosyl-1-benzo[b] furan is a new compound.</p>

Studies on the chemical constituents in root of Gentiana macrophylla from Shaanxi / 中国中药杂志

<p><b>OBJECTIVE</b>To in vestigate the constituents in root of Gentiana macrophylla.</p><p><b>METHOD</b>Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data (UV, IR, MS, 1H-, 13C-NMR etc.) and identified by comparing with standard substance.</p><p><b>RESULT</b>Eight compounds were identified. Four compounds isolated from the chloroform fraction are: 5-carboxyl-3,4-dihydrogen-1H-2-benzopyran-1-one (1), erythrocentauric acid (2), roburic acid (3), oleanolic acid (4). Water fraction gave four known secoiridoid glucosides. They were: gentiopicroside (5), swertiamarine (6), sweroside (7), 6'-O-beta-D-glucosylgentiopicroside (8).</p><p><b>CONCLUSION</b>1 is a novel compound. It was named as erythrocentauric acid. 2 was isolated from genus Gentiana and 8 was isolated from G. macrophylla for the first time.</p>

Isolation and identification of a novel ellagitannin from Phyllanthus urinaria L / 药学学报

<p><b>AIM</b>To investigate the chemical constituents of Phyllanthus urinaria L.</p><p><b>METHODS</b>Various chromatographic techniques were employed for the isolation and purification. The structure was elucidated by spectral analyses.</p><p><b>RESULTS</b>A novel ellagitannin named phyllanthusiin G was isolated, its structure was established as 1-O-galloyl-2-phyllanthoyl-3,6-(R)-HHDP-beta-D-glucose.</p><p><b>CONCLUSION</b>Phyllanthusiin G is a new compound.</p>

Structure identification of jujuboside E / 药学学报

<p><b>AIM</b>To study the chemical constituents of the seeds of Ziziphus jujuba Mill var. spinosa (Bunge) Hu ex. H.F. Chou.</p><p><b>METHODS</b>To separate the constituents by using various kinds of chromatography methods and identify their structures on the basis of spectral analysis.</p><p><b>RESULTS</b>Seven compounds were isolated. Their structures were established as jujuboside E (1), jujuboside B (2), jujuboside A (3), betulic acid (4), stearic acid (5), sucrose (6) and inosine (7).</p><p><b>CONCLUSION</b>Compound 1 is a new compound named jujuboside E. Compounds 5, 6, 7 were isolated for the first time from this plant.</p>

Studies on chemical constituents in fruits of Tibetan medicine Phyllanthus emblica / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of Tibetan medicine Phyllanthus emblica.</p><p><b>METHOD</b>Various chromatographic techniques were employed for isolation and purification of the constituents, and the structures were elucidated by chemical and spectral analyses.</p><p><b>RESULT</b>11 compounds were isolated and identified as gallic acid (I), ellagic acid (II), 1-O-galloyl-beta-D-glucose (III), 3,6-di-O-galloyl-D-glucose (IV), chebulinic acid (V), quercetin (VI), chebulagic acid (VII), corilagin (VIII), 3-ethylgallic acid (3-ethoxy-4,5-dihydroxy-benzoic acid, IX), isostrictiniin (X), 1,6-di-O-galloyl-beta-D-glucose (XI).</p><p><b>CONCLUSION</b>3-Ethylgallic acid (3-ethoxy-4,5-dihydroxy-benzoic acid) is a novel compound, and isostrictiniin was found from P. emblica for the first time.</p>

Studies on the chemical constituents from the aerial parts of Cimicifuga dahurica / 药学学报

<p><b>AIM</b>To seek for new bioactive constituents from the aerial parts of Cimicifuga dahurica.</p><p><b>METHODS</b>Various column chromatographic techniques were employed for the isolation and purification of the ingredients. The structures were elucidated on the basis of 1H, 13CNMR, 1H-1H COSY, HMQC, NOESY and HMBC spectra and chemical reactions.</p><p><b>RESULTS</b>Two cyclolanostanol xylosides, cimidahuside C and D were isolated from the EtOAc section of EtOH extracts.</p><p><b>CONCLUSION</b>Cimidahuside C(1) and D(2) are new triterpenoid xylosides.</p>