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Objective To understand the molecular characteristics of Streptomycin(SM)resistance in multidrug-resistant tuberculosis(MDR-TB)in Jiangxi Province,and to explore the relationship between SM resistant genes(rpsL,rrs and gidB)mutations and SM resistant phenotypes in Beijing genotype TB.Methods 106 non-replicated MDR-TB isolates were collected from Gaoxin Branch of The First Affiliated Hospital of Nanchang University and Jiangxi Provincial Chest Hospital from January to December 2021,and tested for drug-resistance phenotypes,whether they were Beijing genotype or not and the characteristics of rpsL,rrs and gidB gene mutations.Chi-square test was performed to determine whether rpsL,rrs and gidB mutations were related to genotypes and drug-resistance phenotypes.Results Among 106 cases of MDR-TB,76 cases were resistant to SM.A total of 58 cases had rpsL 43A>G mutation,8 cases had 88A>G mutation,5 cases had rrs mutation,and 3 cases had gidB mutation.Statistical analysis showed that the coincidence rate of gene mutation and phenotypic drug-resistance detection was 89.6%,and the specificity and sensitivity were 86.7%and 90.8%,respectively.The isolated rate of Beijing genotype TB was 88.7%,and the drug-resistant gene mutations were mainly concentrated in rpsL and rrs,while the drug-resistant mutations of non-Beijing genotype were mainly concentrated in gidB;in addition,Beijing genotype bacteria were more prone to gene mutations(P = 0.013),but there was no difference in phenotypic drug-resistance.Conclusions Mutations in rpsL,rrs,and gidB genes have a good coincidence rate with phenotypic drug-resistance,and molecular biology can be used to detect directly drug-resistance genes to predict bacterial resistance;TB genotypes are strongly associated with streptomycin resistance characteristics.
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Objective To understand the carriage of NDM-1 and other carbapenemases in carbapenem-resistant Acinetobacterbaumannii(CRAB)in Jiangxi area,and provide laboratory basis for the prevention and control of healthcare-associated infection (HAI). Methods Sixty-four strains of CRAB isolated from clinical specimens from 3 tertiary first-class hospitals in Jiangxi area from January 2015 to June 2016 were collected,susceptibility to com-monly used antimicrobial agents were detected with Kirby-Bauer method. Carbapenemases and metalloenzyme in CRAB were screened with modified Hodge test and EDTA-disk synergy test respectively,carbapenems gene was de-tected by polymerase chain reaction (PCR),NDM-1-producing Acinetobacterbaumannii (A. baumannii)were per-formed conjugation test.Results The resistance rates of CRAB to ampicillin/sulbactam,ciprofloxacin,gentamicin, and levofloxacin were up to 95.31% ,98.44% ,90.63% ,and 54.69% respectively. The positive rates of modified Hodge test and EDTA-disk synergy test were 76.56% and 96.88% respectively. PCR amplification result showed that 87.50% (n= 56)of CRAB carried OXA-23 and VIM-1 genes,18.75% (n= 12)carried SIM,3.13% (n= 2)car-ried OXA-24,and 26.56% (n= 17)carried NDM-1 . CRAB carrying NDM-1 gene were all from The First Affilia-ted Hospital of Nanchang University,64.70% (11/17)of which were pandrug-resistant strains. Conjugation test re-sult showed that NDM-1-producing strains could transfer NDM-1 gene to recipient strain Escherichiacoli J53,then acquired resistance to imipenem. Conclusion Antimicrobial resistance rates of clinically isolated CRAB in this area are high,OXA-23 and VIM-1 genes are the main carbapenemase genes,NDM-1 gene positive CRAB is detected, and there may be a clonal spread of NDM-1 gene in hospital,effective measures should be taken as soon as possible to prevent and control the spread of NDM-1 positive CRAB.
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OBJECTIVE To explore the drug-resistant situation and patients′ basis condition of clinical isolated Enterococcus from Jan to Dec in 2008 to offer evidence for drug-resistant monitoring and clinical antibiotics usage.METHODS All clinical specimens were isolated and cultured and identification conform to Standard′ Operation.The bacteria were identified by using the automatic microorganism analyzer VITEK-2,and bacteria′s drug susceptibility tests were performed using counterparts panel.RESULTS 273 strains Enterococci were isolated,of which E.faecalis were 158 strains,E.faecium were 109 strains,the others were 6 strains.The isolated HLAR E.faecalis were 80 strains and HLAR E.faecium were 10 strains,the isolated.ratio of HLAR was different(P
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OBJECTIVE To explore the drug-resistant situation of clinical isolated Escherichia coli in our hospital from Jun to Dec in 2007,the aim is to offer evidence for drug-resistant monitoring and clinical antibiotics usage in our hospital. METHODS All clinical specimens isolated and cultured from patients were identified by using the automatic microorganism analyzer VITEK-2 as well as bacteria's drug susceptibility tests were performed using counterparts panel. RESULTS A total of 352 strains E. coli were isolated. The isolated ratio of extended spectram-?-lactamases (ESBLs) producing E. coli was 66.2% (233/352),no ESBLs producing E. coli was 33.8% (119/352). From them 111 strains E. coli were isolated in sputum and 89 strains were ESBLs producing and 22 strains were no ESBLs producing; 111 strains E. coli were isolated in urine and 62 strains were ESBLs producing and 49 strains were no ESBLs producing. The drug-resistance difference was obvious between them as well as between strains isolated from different sites. Better to select piperacillin/tazobactam,cefotetan,ertapenem,imipenem,amikacin,and nitrofurantoin to cure all isolated E. coli infection.CONCLUSIONS The drug-resistance of ESBLs producing E. coli is severe (66.2%) so that hospital administers should strengthen antibiotics usage management and improve antibiotics rational usage,inorder to decrease occurrance of bacterial resistance.