Application of digital prosthesis in the repair of palatal fistula in rabbits / 中国组织工程研究

BACKGROUND: Digital impression technology has been widely used in the oral field. Compared with traditional impression technology, it is more convenient and accurate. OBJECTIVE: To explore the application of digital prosthesis for repairing palatal fistula in rabbits. METHODS: The rabbit models of palatal fistula were established by surgery. One month later, 10 rabbit models of palatal fistula were scanned by digital intraoral scanning system. The medical silicone rubber prosthesis for palatal fistula was manufactured by digital impression technology and digital design. After wearing the prosthesis, SUN LB, LAN YY, ZHOU HY, HUY. Application of digital prosthesis In me repair of palatal fistula In rabbits.  Zhongguo Zuzhi Gongcheng Yanjiu. 2020;24(14):2192-2196. DPI:10.3969/j.issn.2095-4344.2228 the accuracy, retention and sealing properties of the prosthesis were examined. The study was approved by the Laboratory Animal Ethical Committee of Hospital of Stomatology, Southwest Medical University. RESULTS AND CONCLUSION: After digital prosthesis repaired palatal fistula, the accuracy, retention and sealing properties of the prosthesis were good. To conclude, the digital prosthesis for palatal fistula repair by digital impression technology achieves accurate repair, and has certain clinical values.

Application of a modified paramedian lower lip-submandibular approach in maxillary (subtotal) total resection / 华西口腔医学杂志

OBJECTIVE@#To investigate the clinical efficacy of a modified paramedian lower lip-submandibular approach for maxillary (subtotal) total resection.@*METHODS@#Eleven patients of maxillary tumors underwent maxillary (subtotal) total resection through the modified paramedian lower lip-submandibular approach. Clinical follow-up visits were conducted to evaluate appearance restoration, facial nerve functional status, parotid gland functional status, and orbital region complication.@*RESULTS@#During the follow-up period of 6-36 months, the appearance of all 11 patients recovered well. All cases presented hidden scars. No facial nerve and parotid duct injury, lower eyelid edema, lower eyelid ectropion, or epiphora in all cases was observed.@*CONCLUSIONS@#Applying modified paramedian lower lip-submandibular approach to maxillary (subtotal) total resection effectively reduces incidence of orbital region complications including lower eyelid edema, lower eyelid ectropion, and epiphora, which often occur to traditional approach. The modified approach produces more subtle scars than other methods and should be applied to treatment of maxillary (subtotal) total resection.

Comparative metabonomics study on urine in rat treated by Angelica sinensis volatile oil / 中国中药杂志

Metabonomics was employed to investigate the effect of Angelica sinensis volatile oil (ASVO) to the endogenous metabolites of normal rats, and to reveal the possible ways of metabolism in rats caused by ASVO. The fifty male Waster rats were randomly divided into five groups (each consists of 10 rats), such as control group, high dose group of ASVO, middle dose group of ASVO, low dose group of ASVO, and Aspirin group. They were given 0.9% saline, 0.352 mL x kg(-1) ASVO, 0.176 mL x kg(-1) ASVO, 0.088 mL x kg(-1) ASVO and ASP respectively with the equal volume of 0.2 mL. Drugs and vehicle were given for 3 successive days. The urine was collected at 12, 24, 36, 48 h after modeling with metabolic cages. Rat urine metabolic fingerprint in different stages was analyzed using GC-MS, based on which the principal component analysis (PCA)and orthogonal partial least-squares discriminant analysis (OPLS-DA) models were established for metabonomic analysis. Potential biomarkers were screened by using variable importance in the projection (VIP) and T test. It was revealed that the middle dose of ASVO at 36 h induces a substantial change in rat urine. Compared with control group, seven kinds of endogenous metabolites in ASP group and ASVO group change significantly (P < 0.05), among which aconitic acid, succinic acid, citric acid, alpha-ketone glutaric acid, glycine and malic acid content had an upward trend (P < 0.05) and prostaglandin content had a downward trend (P < 0.01). The mechanism of ASVO and ASP have the similarity. It is likely that ASVO intervenes the metabolic process by affecting the energy, amino acid and lipid metabolism. Our work also indicates that rats administrated with ASVO can increase the energy metabolism of the body, induce the production of inflammatory substances and strengthen the body's immune ability. The result has also provide a proof for futher interpret ASVO pharmacological effects.

Predictive value of tumor vessel density from enhanced spiral CT scan for lymph node and distant metastasis of colorectal cancer / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To test whether the tumor vessel density (TVD) from the enhanced spiral CT can preoperatively predict nodal status and distant metastasis of colorectal cancer.</p><p><b>METHODS</b>Forty cases of colorectal cancer patients who received surgical treatment were included in this study. The three dimensional tumor vessels were reconstructed by an enhanced CT 64-slice spiral CT and its AW4.4 image processing platform. The TVD was measured by the 1000 high-resolution color graphics pathological analysis system. The TVD level was compared between different tumor size, classification, and TNM stage. The postoperative pathological staging was taken as golden standard.</p><p><b>RESULTS</b>The sensitivity, specificity and accuracy for direct prediction of lymph node metastasis by the enhanced CT 64-slice spiral CT was 74.1%(20/27), 53.8%(7/13) and 67.5%(27/40) respectively. The TVD from the reconstructed three dimensional tumor vessels in the group with lymph node metastasis was significantly higher than that without metastasis(0.070±0.046 vs. 0.037±0.013, P<0.05). The TVD in the distant metastasis group was significantly higher than that without distant metastasis (0.130±0.032 vs. 0.049±0.030, P<0.01). No difference of TVD was found between different tumor size, invasion depth, and differentiation type.</p><p><b>CONCLUSION</b>TVD level from the reconstructed three dimensional tumor vessels can indicate lymph node and distant metastasis of colorectal cancer.</p>

Negative indirect hemagglutination [IHA] test neurocysticercosis in a buddhist's cerebellar hemisphere

Neurocysticercosis [NCC] is expected to be the most common parasitic disease of humans' central nervous system, immunological tests and epidermiological data as well as clinical manifestations and neuroimagings are helpful to make the diagnosis. We report a case of NCC in a buddust's cerebellar hemisphere, the indirect hemagglutination tests were negative, craniotomy was done and two transparent cysts with scolexes were removed, the patient was discharged with good outcome. Histological findings confirmed the diagnosis of NCC. NCC in cerebellar hemisphere is rare, it is more difficult to diagnose when immunological tests are negative, therefore, multiple tools are expected to develop diagnosis and evaluate the efficacy of treatment

Incidental direct carotid-cavernous fistula in a patient with high-grade internal carotid artery stenosis

The concurrent occurrence of internal carotid artery [ICA] stenosis and carotid-cavernous fistula [CCF] is infrequent. We report the case of a 59-year-old man with symptomatic high-grade stenosis of left ICA who was referred to our hospital for surgical treatment. An ipsilateral direct CCF was found incidentally during operation. Ultimately, the two lesions were successfully treated with a covered stent while the ICA was preserved. The result of our study may provide further insight into this rare combination of diseases

Colonic polyps: application value of computer-aided detection in computed tomographic colonography / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Colonic polyps are frequently encountered in clinics. Computed tomographic colonography (CTC), as a painless and quick detection, has high values in clinics. In this study, we evaluated the application value of computer-aided detection (CAD) in CTC detection of colonic polyps in the Chinese population.</p><p><b>METHODS</b>CTC was performed with a GE 64-row multidetector computed tomography (MDCT) scanner. Data of 50 CTC patients (39 patients positive for at least one polyp of ≥ 0.5 cm in size and the other 11 patients negative by endoscopic detection) were retrospectively reviewed first without computer-aided detection (CAD) and then with CAD by four radiologists (two were experienced and another two inexperienced) blinded to colonoscopy findings. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of detected colonic polyps, as well as the areas under the ROC curves (Az value) with and without CAD were calculated.</p><p><b>RESULTS</b>CAD increased the overall sensitivity, specificity, positive predictive value, negative predictive value and accuracy of the colonic polyps detected by experienced and inexperienced readers. The sensitivity in detecting small polyps (5 - 9 mm) with CAD in experienced and inexperienced readers increased from 82% and 44% to 93% and 82%, respectively (P > 0.05 and P < 0.001). With the use of CAD, the overall false positive rate and false negative rate for the detection of polyps by experienced and inexperienced readers decreased in different degrees. Among 13 sessile polyps not detected by CAD, two were ≥ 1.0 cm, eleven were 5 - 9 mm in diameter, and nine were flat-shaped lesions.</p><p><b>CONCLUSIONS</b>The application of CAD in combination with CTC can increase the ability to detect colonic polyps, particularly for inexperienced readers. However, CAD is of limited value for the detection of flat polyps.</p>

Expression of Aurora-B in human glioma tissue and its significance / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the expression of Aurora-B in human glioma tissue and its significance.</p><p><b>METHODS</b>The total RNA was extracted from 41 human glioma tissues and 11 normal brain tissues by Trizol reagent. After reverse transcription of the total RNA into cDNAs, Aurora-B mRNA expressions in these samples were detected by quantitative real-time PCR. The protein expression in these samples was detected using immunohistochemical staining.</p><p><b>RESULTS</b>Aurora-B mRNA and protein expressions were significantly increased in glioma tissues as compared with those in normal brain tissues.</p><p><b>CONCLUSION</b>Aurora-B mRNA and protein show markedly higher expressions in glioma tissue, suggesting that Aurora-B may be one of the malignant biomarkers in the pathogenesis and progression of human glioma.</p>