Effect of Sea Cucumber Enzymolysis Fermentation Liquid on immunosuppressed mice based on metabonomics / 中国药理学通报

Aim To prepare the sea cucumber enzy¬molysis fermentation liquid (SCEFL) by enzymatic hydrolysis of protease and fermentation of probiotics and to investigate the effect of SCEFL on the immunosup-pression induced by cyclophosphamide in mice and to explore its mechanism by metabomic method. Methods The immunosuppressive model was induced by in-traperitoneal injection of cyclophosphamide. C57BL/6J mice were randomly divided into normal group, model group, Levamisole group, SCEFL groups (at low, medium and high doses). The pathological changes of spleen were observed by HE staining. The proportion of CD4

Systematic training in traditional martial arts choreography in college / Treinamento sistemático em coreografias de artes marciais tradicionais na universidade / Entrenamiento sistemático en coreografías de artes marciales tradicionales en la universidad

ABSTRACT Introduction Martial arts choreography is a sporting event of high intensity and speed. The energy metabolism of its practitioners is characterized mainly by anaerobic consumption, complemented by aerobic metabolism. Martial arts routines have particular movement rules, and the systematic training method has played a positive role in developing martial arts routines. Objective This paper explores the role of eight-week systematic training in martial arts routines. Methods 36 martial arts choreography players from colleges and universities were selected by random sampling. The above research subjects were randomly divided into experimental and control groups. The experimental group adopted an exclusive training protocol. The control group was trained traditionally. Results Significant differences were found between the two groups in the test results of eight-speed indicators. A significant difference was also found between the two groups in the test data of the three endurance indicators. Conclusion The personalized training method used in this paper significantly improved athletes' speed and endurance levels. The research results of this paper can provide a reference for routine martial arts staff in improving the professional athletic technical level. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução A coreografia das artes marciais é um evento esportivo de alta intensidade e velocidade. O metabolismo energético de seus praticantes é caracterizado principalmente pelo consumo anaeróbico, complementado pelo metabolismo aeróbico. As rotinas das artes marciais têm suas próprias regras de movimento particulares e o método de treinamento sistemático tem desempenhado um papel positivo no desenvolvimento das rotinas das artes marciais. Objetivo Este artigo explora o papel do treinamento sistemático por 8 semanas nas rotinas de artes marciais. Métodos 36 jogadores de coreografia em artes marciais de faculdades e universidades foram selecionados por amostragem aleatória. Os sujeitos da pesquisa acima foram divididos aleatoriamente em dois grupos: experimental e controle. O grupo experimental adotou um protocolo exclusivo de treinamento. O grupo de controle foi treinado da maneira tradicional. Resultados Foram encontradas diferenças significativas entre os dois grupos nos resultados dos testes de oito indicadores de velocidade. Também se observou diferença significativa entre os dois grupos nos dados de teste dos três indicadores de resistência. Conclusão O método personalizado de treinamento utilizado neste trabalho demonstrou um efeito de destaque na melhoria da velocidade e do nível de resistência dos atletas. Os resultados da pesquisa deste artigo podem fornecer uma referência para a equipe de rotina de artes marciais no aprimoramento do nível técnico profissional atlético. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción La coreografía de las artes marciales es un evento deportivo de alta intensidad y velocidad. El metabolismo energético de sus practicantes se caracteriza principalmente por el consumo anaeróbico, complementado por el metabolismo aeróbico. Las rutinas de las artes marciales tienen sus propias reglas de movimiento y el método de entrenamiento sistemático ha desempeñado un papel positivo en el desarrollo de las rutinas de las artes marciales. Objetivo Este trabajo explora el papel del entrenamiento sistemático durante 8 semanas en las rutinas de las artes marciales. Métodos Se seleccionaron 36 coreógrafos de artes marciales de colegios y universidades mediante un muestreo aleatorio. Los sujetos de la investigación mencionados se dividieron aleatoriamente en dos grupos: experimental y de control. El grupo experimental adoptó un protocolo de entrenamiento exclusivo. El grupo de control fue entrenado de forma tradicional. Resultados Se encontraron diferencias significativas entre los dos grupos en los resultados de las pruebas de ocho indicadores de velocidad. También se observó una diferencia significativa entre los dos grupos en los datos de las pruebas de los tres indicadores de resistencia. Conclusión El método de entrenamiento personalizado utilizado en este trabajo demostró un efecto destacado en la mejora del nivel de velocidad y resistencia de los atletas. Los resultados de la investigación de este trabajo pueden proporcionar una referencia para el equipo de artes marciales de rutina en la mejora del nivel técnico atlético profesional. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Cross-lagged analysis of passive social network site use and fear of missing out in college students / 中国学校卫生

Objective@#In order to explore the mutual predictive relationship between passive social network site use and fear of missing out.@*Methods@#A one-year longitudinal design was adopted. 652 college students were convinently selected from 2 colleges in Heilongjiang Province measured twice by Passive Social Network Site Use Scale (PSNSUS) and Fear of Missing Out Scale (FOMOS). The structural equation model was used to conduct cross-lagged analysis.@*Results@#There was a significant positive correlation between PSNSUS and FOMOS scores measured twice(r=0.44，0.45, P<0.01). The results of cross-lagged analysis indicated that, after controlling for the correlation between PSNSUS and FOMOS at time 1 and time 2, pre-test PSNSUS had a significant effect on post-test FOMOS (β=0.55，P<0.01), and the predictive effect was stronger among female students. pre-test FOMOS has a significant effect on post-test PSNSUS (β=0.11, P<0.05), and the predictive effect was stronger among male students.@*Conclusion@#It suggests that passive social network site use and fear of missing out could predict eachother in college students.

Safety and effectiveness of video-assisted thoracoscopic surgery pneumonectomy for bronchiectasis / 中国胸心血管外科临床杂志

@#Objective    To explore the safety and effectiveness of video-assisted thoracoscopic surgery (VATS) pneumonectomy for bronchiectasis. Methods    The clinical data of 164 patients undergoing VATS pneumonectomy or open thoracotomy for bronchiectasis in our hospital from March 2002 to July 2012 were retrospectively analyzed. Patients were divided into two groups according to different surgical methods: a thoracotomy group (122 patients, 63 males, 59 females) and a thoracoscopic surgery group (42 patients, 15 males, 27 females). Surgical and follow-up indicators were compared between the two groups. Results    There was no difference between the two groups in the blood loss, operation time, perioperative mortality or complication. However patients undergoing VATS had shorter length of postoperative stay than those undergoing thoracotomy (6.9±2.6 d vs. 8.1±3.1 d, P=0.030). In the thoracoscopic surgery group, 3 patients were lost to follow-up and in the thoracotomy group, 5 patients were lost to follow-up. In a median follow-up of 51 months (ranging from 2 to 116 months), 36 patients (92.3%) fully recovered with no sputum or haemoptysis and 3 (7.7%) partially recovered with a reduced sputum or haemoptysis in the thoracoscopic surgery group; 105 (89.7%) fully recovered with no sputum or haemoptysis, 10 (8.5%) partially recovered with a reduced sputum or haemoptysis while 2 (1.7%) without any improvement in the thoracotomy group with no statistical difference (P=0.700). Conclusion    VATS pneumonectomy for bronchiectasis is equivalent to thoracotomy in terms of safety and effectiveness, and can be used as an alternative surgical procedure for the treatment of bronchiectasis.

Clinical implementation of robot assisted trans-subxiphoid (extended) thymectomy / 中国胸心血管外科临床杂志

@#Objective    To present the preliminary clinical experience of robot assisted trans-subxiphoid (extended) thymectomy in patients with thymic neoplasms or myasthenia gravis. Methods    A total of 62 patients (34 males and 28 females at an average age of 38±11 years) suffering from thymic neoplasms or myasthenia gravis who underwent robotic (extended) thymectomy via subxiphoid approach were included in our department between August 2016 and August 2017. All of the operation were completed through 4 ports. In details, the observation hole was created just below the xiphoid process, two ports for arm 1 and arm 2 were created below bilateral subcostal arch at the midclavicular line, and trocar for arm 3 was placed in the 5th or 6th intercostal space at the anterior axillary line, respectively. Patients with thymic neoplasms received thymectomy. Patients with myasthenia gravis received extended thymectomy. Results    All the patients experienced uneventful operations. The mean operative time was 116.0±34.0 min. The mean intraoperative blood loss was 5.6±4.3 ml. The mean postoperative hospital stay was 4.0±2.2 days. There was no intra-operational massive hemorrhage, mortality, conversion or postoperative complication during the postoperative and follow-up period. Conclusion    Robotic trans-subxiphoid thymectomy is safe and feasible, which is a promising technique for extensive application.

Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity

ABSTRACT Objective: To establish the detrusor overactivity (DO) model induced by visceral hypersensitivity (VH) and investigate the relationship between mast cell (MC) infiltration and DO. Materials and Methods: Sixty rats are divided into 4 groups randomly: Group 1:Baseline group; Group 2: DO group; Group 3: CON group; Group 4: VH group. The colorectal distension (CRD) and abdominal withdral reflex (AWR) scores are performed to evaluate VH. The cystometric investigation and histological test of MC infiltration are assessed. Results: The threshold pressure of CRD in the VH group is significantly lower than that in the CON group (P<0.001). At the distension pressure ≥20 mmHg, the AWR scores of the VH group are significantly higher than those of the CON group (10 mmHg: P=0.33; 20 mmHg: P=0.028; 40 mmHg: P<0.001; 60 mmHg: P<0.001; 80 mmHg: P<0.001). DO model is successfully established in the VH group (DO rate=100%). Compared with the CON group, the numbers of MC infiltration are significantly increased in the VH group, including submucosa of bladder (P<0.001), mucosa lamina propria/mesentery of small intestine (P<0.001), and mucosa lamina propria/mesentery of large intestine (P<0.001). Furthermore, more MC activation as well as degranulation are observed in the VH group. Conclusions: It is indicated that DO model can be established in the VH rats. The MC infiltration may play an important role in DO induced by VH, and may be helpful to understand the mechanisms of DO in VH patients.

TGF-ß1 expression is associated with invasion and metastasis of intrahepatic cholangiocarcinoma

BACKGROUND: Transforming growth factor (TGF)-ß is involved in many physiologic processes, it often promotes metastasis, and its high expression is correlated with poor prognosis. In the present study, we analyzed the correlation between transforming growth factor beta 1 (TGF-ß1) expression and prognosis in intrahepatic cholangiocarcinoma RESULTS: We examined the expression of TGF-ß1 in 78 intrahepatic cholangiocarcinomas by immunohistochemistry and correlated the expression with clinicopathological parameters. TGF-ß1 was expressed in 37 of 78 (47.4 %) intrahepatic cholangiocarcinomas. The expression of TGF-ß1 was significantly correlated with lymph node metastasis, distant metastasis, and tumour recurrence. Patients with TGF-ß1-positive tumours had significantly shorter survival time. In a multivariant analysis, the expression of TGF-ß1 and the tumour stage were independent prognostic factors CONCLUSIONS: Our data suggest that expression of TGF-ß1 is a novel prognostic marker for intrahepatic cholangiocarcinoma.

Improved model for the establishment and evaluation of detrusor overactivity in female Wistar rats

Objective To improve the model for establishment and evaluation of detrusor overactivity in female Wistar rats. Materials and Methods We ligated the perineal urethra of female Wistar rats and then performed filling cystometry. The probability of detrusor overactivity, bladder capacity, peak voiding pressure and histological changes were investigated. Results Detrusor overactivity ratio of the obstruction group was 32.4%. Bladder capacity increased from 0.273 ± 0.036mL in control group to 0.89 ± 0.19mL in detrusor overactivity group (P < 0.001), and peak voiding pressure increased from 45.9 ± 4.1 cm.H2O to 63.5 ± 17.4cm.H2O (P = 0.007). For obstruction group, compared to no detrusor overactivity rats, detrusor overactivity rats had higher bladder capacity (0.89 ± 0.19mL versus 0.43 ± 0.09mL, P < 0.001) and higher peak voiding pressure (63.5 ± 17.4cm.H2O versus 44.8 ± 6.2cm.H2O, P = 0.005). Detrusor overactivity rats were classified according to peak voiding pressure (49.2 ± 4.2cm.H2O versus 80.8 ± 7.1cm.H2O, P < 0.001). Moreover, bladder weight increased significantly in detrusor overactivity rats (P = 0.003, P = 0.028) and detrusor histological hypertrophy was observed. Conclusions Ligating perineal urethra and filling cystometry with intra-urethral cannula approach is a simple and easily reproducible method to establish and evaluate the model of detrusor overactivity in rats. .

Research progress on effects of traditional Chinese medicines on proliferation, apoptosis and differentiation of bone marrow mesenchymal stem cells / 中国中药杂志

Bone marrow mesenchymal stem cells (BMSCs) are a kind of pluripotent stem cells derived from bone marrows, which can not only support hematopoiesis, but also have capabilities of multidifferentiation, high-proliferation and self-renewing. They have become one of hotspots in stem cell studies. Studies on in vitro intervention with BMSCs with TCMs have made remarkable progress in recent years. According to the findings, some traditional Chinese medicines can promote proliferation of BMSCs, some can inhibit the apoptosis of BMSCs, while others can induce BMSCs to differentiate into multiple cell types, such as osteoblast. Furthermore, some studies also involved relevant action mechanisms. The authors summarized the advance in relevant studies by reference to relevant literatures of this field.

Synthesis, structure characterization and anti-tumor activity of lanthanide complex Ln (Phen)2 (5-Fu)3 (NO3) (NO3 )2 / 药学学报

<p><b>AIM</b>To study the biochemistry of lanthanides, the cooperative action of inorganic and organic anti-tumor drugs.</p><p><b>METHODS</b>A series of rare earth complexes were synthesized with Ln(NO3) 6H2O, Phen and 5-Fu. Their anti-tumor activity was measured by the improved MTT, SRB methods.</p><p><b>RESULTS</b>The formula of complex Ln[(Phen)2(5-Fu)3(NO3)](NO3)2(Ln = Y, La, Ce, Sm, Gd, Dy, Er; Phen = 1, 10-phenanthroline; 5-Fu = fluorouracil) was characterized by elemental analyses, molar conductivity, IR, TGA, and 13C NMR spectra. The preliminary biological activity studies indicated that Lanthanide complex has strong anti-tumor activity in vitro.</p><p><b>CONCLUSION</b>The complex might have anti-tumor cooperation action.</p>