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@#Post-mortem microbiology (PMM) is an important tool in identifying possible causes of sudden unexpected death, as an infectious cause is highly suspected. However, contamination is a major problem in microbiology, and this has increased the difficulty determining the true pathogen that contributes to death in post-mortem cases. Skin commensals are common contaminants in blood cultures. This study was conducted to investigate the skin flora on early deceased bodies and observe the bacteria detected at different post-mortem intervals (PMIs). As blood is usually drawn from the neck and femoral sites for PMM examination, the two body sites were chosen as the sampling sites. Skin swab samples from the neck and femoral (n=80) of each early deceased body were collected by sterile cotton swabs. DNA was extracted from the swabs and then subjected to high-throughput 16S rRNA sequencing by using the Illumina MiSeq platform. Staphylococcus was found to be the most dominant genus in both neck and femoral sites. LEfSe results showed that Cutibacterium is significantly different at the neck site while Corynebacterium is more abundant at femoral site. There are significant differences at genus level between PMI<5H and PMI>5H at both neck and femoral sites. The findings of the present study may act as a reference for microbiologists and forensic pathologists when mixed growth or contamination occurs in post-mortem blood cultures.
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Background: Laparoscopic Sleeve gastrectomy [LSG] is the most common restrictive procedure performed worldwide for treatment of morbid obesity
Objective: To assess the safety, effectiveness and complications of LSG performed for Iraqi patients and shows early experience of LSG
Methods: Prospective clinical case series study conducted in Al-Jumhoori Teaching Hospital and involved 70 patients [57 females and 13 males]; their age [19-59 average 37 years] underwent LSG [6 open, 64 laparoscopically with one conversion]; 51 patients finish 1 year follow up
Results: The initial body weight 70-195[122Kg]; BMI 31.8-65.9 [50.16Kg/m[2]]; two patients with diabetes mellitus and BMI less than 35Kg/m[2] involved. The average weight loss assessed and found to be [13.85, 32.8, 40.8 and 48.3 Kg] in 1st, 3rd, 6th and 12th month interval; The BMI reduced from 50.16 to 35.43 and 29.52 at 6th and 12th months. We recorded 1 mortality after 10 days [massive pulmonary embolism], 1 case converted to open and minigastric bypass due to narrowing of the sleeve part. The associated comorbidities improved after weight loss, and the quality of life improved in 88% of patients depending on bariatric analysis and reporting outcome systems [BAROS]
Conclusion: Sleeve gastrectomy is relatively safe and effective procedure to decrease weight for morbid obesity and it improves the quality of life, but all bariatric procedures needs good health resources and settled countries which were not present in Iraq for the time being