Effect of Qingshen granules on miR-23b and PINKl/Parkin pathway in rat NRK-52E cell transdifferentiation model / 中国药理学通报

Aim To investigate the targeting mechanism of miR-23b on PINKl/Parkin pathway in transdifferentiation of NRK-52E cellsinduced by TGF-β1, and to elucidate the intervention mechanism of Qingshen granules drug-containing serum on NRK-52E cell transdifferentiation. Methods Ultra-high performance liquid chromatography ( UPLC ) fingerprinting method was used to analyze Qingshen granules. The NRK-52E transdifferentiation model induced by TGF-β1 was constructed. The NRK-52E cells were divided into simulated no-load control group, miR-23b-5p simulated group, inhibitor no-load control group, and miR-23b-5p inhibitor group, after transfection with siRNA, and the effect of miR-23b-5p on PINK1 expression was ob-served. The NRK-52E cells were then divided into normal group, TGF-(31 group, Qingshen granule group, miR-23 b-mimic group, miR-23 b-mimic group, and miR-23b-mimic + Qingshen granule group. Western blot was used to detect the expression of Pinkl, Parkin, LC3 n, Beclin-1, P62 and a-SMA proteins, and RT- PCR was used to detect the expression of miR-23 b-5p, Pinkl, Parkin, Beclin-1 and a-SMA mRNA in NRK- 52E cells. Dual-Luciferase Reporter gene experiment was used to detect the targeting relationship between miR-23b-5p and PINKL Results UPLC fingerprinting method found 11 active components in Qingshen granules. After overexpression of miR-23b-5p, the expression of PINkl mRNA significantly increased (P 0. 05 ). The experimental results showed that the expressions of miR- 23b-5p, Pinkl, Parkin, Beclin-1, LC3 II and LC3 II/ I ratio in TGF-β1 group were significantly lower than those in normal group, but the expressions of P62 and a-SMA were significantly higher than those in normal group ( P <0.05). The expressions of miR-23 b-5 p, Pinkl, Parkin, Beclin-1, LC3 II and LC3 11/ I ratio in Qingshen granule group and miR-23 b-mimic group were significantly higher than those in TGF-β1 group, and the expressions of P62 and a-SMA were significantly lower than those in TGF-β1 group (P < 0. 05 ). The performance of miR-23 b-mimic + Qingshen granule group was better than that of miR-23 b-mimic group (P < 0. 05 ). Conclusions Qingshen granules can up- regulate the expression of miR-23b-5p in NRK-52E cellsand inhibit the transdifferentiation process of NRK- 52E cells by enhancing the mitochondrial autophagy activity mediated by PINKl/Parkin pathway.

Serotypes and drug resistance of salmonella in commercial food in Pudong New Area from 2020 to 2022 / 上海预防医学

ObjectiveTo understand the serotype distribution and drug resistance of salmonella contaminated in commercially available food. MethodsSalmonella detection， including the serotypes， was conducted in food products sold in Pudong New Area from 2020 to 2022. The antimicrobial susceptibility test of 15 antibiotics was conducted by the broth microassay. ResultsA total of 118 salmonella strains were detected in 2 497 pieces of food， with a total detection rate of 4.7%. The dominant detection categories were poultry meat， livestock meat and aquatic products. The 118 salmonella strains could be divided into 24 serotypes， Salmonella enteritidis （26.4%）， Salmonella Typhimurium （16.2%） and Salmonella delpy （14.4%） were the main dominant types. Salmonella had the highest resistance rate to ampicillin （63.6%）， followed by tetracycline， chloramphenicol， cotrimoxazole and nalidixic acid. Among the three dominant serotypes， the multidrug resistance rate of Salmonella typhimurium was the highest （89.5%）， followed by Salmonella delpy （70.6%） and Salmonella enteritidis （61.3%）. ConclusionLivestock， poultry meat， and aquatic products are seriously contaminated by salmonella with diverse serotypes. The livestock meat is mainly contaminated by Salmonella typhimurium and Salmonella delpy， and the poultry meat is mainly contaminated by Salmonella enteritidis. The drug resistance spectrum is wide and the multi-drug resistance rate is high. Different from the livestock and aquatic isolates， poultry meat-derived strains have high tolerance to ampicillin， nalidixic acid and polymyxin， and carry certain potential food safety risks.

Relationship between immune regulation and structure of polysaccharides / 中国中药杂志

Polysaccharides have significant immunomodulatory activity and have good development value in food and medicine fields. At present, there are many studies on the chemical structure and immune activity of polysaccharides, but the relationship between them of polysaccharides has not been fully explained, which limits the further development and utilization of polysaccharide resources. The immune activity of polysaccharides is closely related to their own structure. This paper systematically summarized the relationship between the relative molecular weight, monosaccharide composition, glycosidic bond types, chemical modification, and advanced conformation of polysaccharides and the immune regulation, aiming to provide references for the profound study of polysaccharide structure-activity relationship and utilization of polysaccharides.

Finite element analysis of hip joint stress distribution based on CT imaging in marathon runners during the gait cycle / 中华放射学杂志

Objective:To explore the distribution of perihip stress in male marathon runners and normal volunteers during the gait cycle using finite element analysis.Methods:In November 2022, the hip joint CT image data of a male marathon runner and a male healthy volunteer was collected. The gait cycle was divided into 7 phases, including the foot to the ground, the early, middle, and late period of one-foot support, the toe push and extension period, and the beginning and end of the swing. A three-dimensional finite element model was constructed based on the gray value of CT and according to the individualized material attribute assigning scheme. The dynamic change of hip joint contact force during the gait cycle was applied to the load, and the biomechanical qualitative analysis of the model was carried out through the equivalent stress results. Based on qualitative analysis, the same 10 nodes on the front, middle, and back of the model acetabulum, the front, and back of the femoral head, and the neck of the femur were extracted as equivalent stress reference points, and the biomechanical quantitative analysis of the model was carried out.Results:During the gait cycle, according to the hip equivalent stress distribution diagram, the contact on the articular surface of the two subjects mainly occurred in the posterior upper part of the acetabulum and the posterior part of the femoral head and gradually moved outward and backward with the joint force during walking. From the point of view of the stress distribution nodes around the hip joint, the stress changes of the nodes in each part of the two subjects showed bimodal characteristics with the load inside the hip joint at each phase. The internal stress value of the hip joint showed a gradual upward trend from the foot to the ground, reached the maximum stress in the middle period of one-foot support, then decreased, and slightly increased in the toe push and extension period. Then, the stress values at the beginning and end of the swing gradually decreased until entering the next gait cycle. The stress values in the posterior acetabulum and posterior femoral head of the marathon runner were smaller than those of the healthy volunteer.Conclusion:The stress around the hip joint was different between marathon runner and healthy volunteer, but the stress distribution and transmission path were the same.

High-value utilization technology and approach of Chinese medicinal residues under background of "Dual Carbon" / 中国中药杂志

In recent years, the traditional Chinese medicine(TCM)industry has experienced rapid development, resulting in a significant amount of Chinese medicinal residues generated during the industrial manufacturing process. Currently, the main methods of handling Chinese medicinal residues include stacking, landfilling, and incineration, which lead to substantial resource waste and potential environmental pollution. With "carbon peak" and "carbon neutrality"( "Dual Carbon")becoming national strategic goals, the TCM industry is ushering in a new wave of "low-carbon" trends, and the high-value utilization of Chinese medicinal residues has become a breakthrough for implementing a low-carbon economy in the TCM sector. From the perspective of a low-carbon economy, this article reviewed literature in China and abroad to summarize the microbial transformation technology, enzymatic conversion technology, biomass pyrolysis, gasification, hydrothermal liquefaction, and other high-value utilization technologies for Chinese medicinal residues. It also overviewed the applications of Chinese medicinal residue in feed additives, organic fertilizers, edible mushroom cultivation substrates, preparation of activated carbon for wastewater treatment, and new energy batteries. Considering the current status of resource utilization of Chinese medicinal residues, feasible strategies and suggestions for resource development and utilization were proposed to improve the quality and efficiency of the Chinese medicinal resource industry chain and promote green development, thereby providing research ideas and theoretical basis for achieving carbon peak and carbon neutrality goals.

Cognition of e-cigarettes and its influencing factors among secondary school personnel in Shanghai / 上海预防医学

ObjectiveTo investigate the cognition of e-cigarette compositions and hazards and its influencing factors among secondary school personnel （junior， senior and vocational high school） in Shanghai， so as to provide ideas for improving the cognition level of e-cigarettes among the school personnel and tobacco control efforts on campus in the future. MethodsA multi-stage stratified cluster random sampling method was used to conduct an anonymous online questionnaire survey among 1 767 secondary school personnel in Shanghai. ResultsThe proportion of school staff who had used e-cigarettes was 3.79%. The participants who were not sure whether the e-cigarette smoke was “mainly water vapor （incorrect）” and whether the e-cigarette smoke “contained nicotine （correct）” and “contained formaldehyde （correct）” accounted for 62.71%， 54.44% and 61.91%， respectively. Among them， 68.48%， 55.46% and 50.37%， respectively， agreed that e-cigarettes would be “addictive”， “harmful” and “that second-hand smoke was harmful”. Binary logistic regression analysis showed the following risk factors for the low level of school personnel’s awareness of e-cigarette compositions： age ≥40 years （OR=1.65， 95%CI： 1.32‒2.06）， working in junior high school （OR=1.37， 95%CI： 1.09‒1.73）， educational attainment level as junior college or below （OR=1.67， 95%CI： 1.02‒2.74）， and not having participated in tobacco control education activities （OR=1.37， 95%CI： 1.08‒1.73）. Meanwhile， working in junior （OR=1.43， 95%CI： 1.12‒1.83） or senior （OR=1.44， 95%CI： 1.08‒1.92） high school， educational attainment level as junior college or below（OR=2.10， 95%CI： 1.24‒3.56）， having used e-cigarettes （OR=2.98， 95%CI： 1.63‒5.42）， not having participated in tobacco control education activities （OR=1.49， 95%CI： 1.16‒1.92） and low level of awareness of e-cigarette compositions （OR=4.24， 95%CI： 3.44‒5.23） were risk factors for the low level of school personnel’s awareness of e-cigarette hazards. ConclusionsThe level of e-cigarette awareness among school personnel in Shanghai is low， especially those who are older， had low educational attainment level and had used e-cigarettes. In the future， tobacco control education for school personnel should be strengthened to improve their cognition and ability of tobacco control on campus.

The function of auditory cortex in the elderly using functional near-infrared spectroscopy technology / 中华耳鼻咽喉头颈外科杂志

Objective: Functional near-infrared spectroscopy (fNIRS) was used to study the effect of aging on the neuroimaging characteristics of cerebral cortex in the process of speech perception. Method: Thirty-four adults with normal hearing were recruited from March 2021 to June 2021, including 17 in the young group, with 6 males, 11 females, age (32.1±5.0) years, age range 20-39 years. and 17 in the elderly group, with 6 males, 11 females, age (63.2±2.8) years, age range 60-70 years. The test material was the sentence table of the Mandarin Hearing Test in Noise (MHINT). The task state block experiment design was adopted, and the temporal lobe, Broca's area, Wernicke's area, motor cortex were used as regions of interest. Objective brain imaging technology (fNIRS) combined with subjective psychophysical testing method was used to analyze the activation area and degree of cerebral cortex related to auditory speech perception in the elderly and young people under different listening conditions (quiet, signal-to-noise ratio of 10 dB, 5 dB, 0 dB, -5 dB). SPSS 23 software was used for statistical analysis. Result: The activation area and degree of activation in the elderly group were lower than those in the young group under each task condition; The number of activation channels in the young group were significantly more than those in the old group, and the number of activation channels in the left hemisphere were more than those in the right hemisphere, but there was no difference in the number of activation channels. There were more channels affected by age in the left hemisphere than in the right hemisphere. The activation degree of the young group when the signal-to-noise ratio was 0 dB was significantly higher than that of other signal-to-noise ratio conditions (P<0.05), but there was no significant difference in the old group under the five conditions (P>0.05). The speech recognition score of the young group was higher than that of the old group under all conditions. When the quiet and signal-to-noise ratio was 10 dB, the correct score of the two groups was equal or close to 100%. With the gradual decrease of signal-to-noise ratio, there was a significant difference between the two groups when the signal-to-noise ratio was 5 dB. The speech recognition accuracy of the young group decreased significantly when the signal-to-noise ratio was 0 dB, while that of the old group decreased significantly when the signal-to-noise ratio was 5 dB. Conclusions: With the increase of age, the speech perception in noisy environment and the activity of cerebral cortex gradually deteriorate, and the speech dominance hemisphere (left hemisphere) will be significantly affected by aging. The overall activation area and activation degree of the elderly under different speech tasks are lower than those of the young.

Restorative effects of different forest environments in spring, summer, and autumn / 环境与职业医学

Background It has been proved by many studies that forest environment has good restorative effects. Some researchers have attempted to identify natural factors affecting restorativeness. However, current studies have not focused on the effect of seasonal variation of forest environments on its restorative strength. Objective To evaluate the restorative effect changes of two types of forest environments across different seasons. Methods The experiments were conducted in April (spring), June (summer), and October (autumn) of 2017. Forty subjects were recruited in spring，18 in summer, and 20 in autumn. In spring, the subjects were randomly assigned to a forest park in suburban area and one in semi-pristine area in Beijing where each subject only experienced one kind of forest environment. In summer and autumn, each subject experienced two kinds of forest environments. They spent the first day in the suburban area forest park, and the second day in the semi-pristine area forest park. First the subjects completed the Brief Profile of Mood States (BPOMS) and had their heart rate been monitored for 10 min before test followed by a 30 min forest experience with sedentary sitting. At the end of each experience, the subjects completed the BPOMS again, and also the Restoration Environment Scale-Chinese version. Objective physical environment indicators were monitored simultaneously during forest experience. The changes of each indicator were analyzed before and after forest experience in selected three seasons. Results In the objective physical environment indicators, the concentrations of anion in selected three seasons in the semi-pristine area forest were higher than the values in the suburban development area forest; the concentration of anion in autumn was much higher than those in the other two seasons. In the assessment of the Restorative Environment Scale, the total scores of restorativeness of the semi-pristine area forest were generally higher than the scores in the suburban development area forest, but significant differences were found only in spring and summer (P < 0.001 in spring, P=0.022 in summer, and P=0.083 in autumn). In terms of physiological indicators, the average heart rate was decreased, while short-term R-R interval standard deviation (SD1) and root mean square of difference of successive intervals (RMSSD) were increased after the designed forest experience sessions in the three seasons, and there was statistical difference between forest types. In terms of psychological indicators, a decrease in the score of total mood disorder was presented after the experience session. In spring, there was a trend that the forest park in suburban development area had a better regulation effect on total mood disorder (P=0.07). In summer and autumn, there was a trend that the semi-pristine area forest park had a better regulation effect on total mood disorder (P=0.062 in summer and P=0.001 in autumn). Conclusion Generally, forest environments can improve the psychological and physiological indicators of human, and the improvement varies with frosts types and seasons. The restorative effects of forests in semi-pristine areas is better than those in suburban development area.

Differential expression and bioinformatics analysis of epsin3 in colorectal cancer / 解剖学报

Objective To investigate the expression and significance of the adaptor protein epsin 3 (EPN3) in colorectal cancer in order to provide reference for further stud)' of EPN3. Methods GEPIA and GEDS were used to analyze the expression of EPN3 in colorectal cancer tissues and cells. SMART and cBioPortal databases were used to analyze the relationship between EPN3 gene metfrylation and cop)' number variation and its expression level. Metascape was used to complete analysis of gene ontology functional annotation and related pathways of EPN3 related genes and BioPlex was applied to construct a protein network in HCT116 cell. Thirteen pairs of colorectal cancer adjacent tissue and cancer tissue specimens were collected, and EPN3 mRNA expression were detected by Real-time PCR. The effect of abilities of cell proliferation, clone formation and migration via silencing EPN3 in HCT116 and HT29 were observed. Results GEPIA, GEDS, SMART and cBioPortal analyses showed that EPN3 was highly expressed in colorectal tumor tissues (P<0. 01), and was related to methylation and copy number variation. The enrichment result of EPN3 related genes showed that it was mainly related to cell adhesion. And a protein interaction network constructed by CCDC130, TNFAIP1, PHGDH, EPN2, etc. was related to protein ubiquitination. Real-time PCR result showed that EPN3 was highly expressed in tumor tissues (P<0. 05). Silencing EPN3 inhibited the proliferation, clony formation and migration abilities of HCT116 and HT29 cells. Conclusion EPN3 is highly expressed in colorectal cancer tissues and is related to cell adhesion and protein ubiquitination. Down-regulated EPN3 can inhibit abilities of proliferation, clony formation and migration of HCT116 and HT29 cells, and this could provide a reference for further research on EPN3.

HPV vaccination intention for female college students: review of current situation and influencing factors / 上海预防医学

The HPV vaccination intention in Chinese female college students and the application of health behavior related theory are reviewed in this article. Based on the health ecological model, influencing factors of college students' HPV vaccination intention were analyzed, proposing application prospect of the information-motivation-behavioral skills model in HPV vaccine behavioral science. Thus is to be explored the behavioral intervention model of Chinese female college students' HPV vaccination.

Analysis of the monitored results of influenza from 2010 to 2014 in Huainan City / 中国基层医药

Objective To analyze the monitored results of the influenza from 2010 to 2014 in Huainan city, and to master the local status of influenza and the dynamic changes of this kind of epidemic,which can get us to find the outbreaks promptly.In the meantime,to provide the scientific basis for the further prevention.Methods 11 600 cases of patients with influenza like disease in Huainan were collected and viral nucleic acid was analyzed by using the method of detecting RealTime RT -PCR.Results From 2010 to 2014,3 633 cases were collected in Huainan General Hospital.PCR showed that 472 cases were positive(the positive rate was 13.0%),of which 187 cases were Seasonal H3 model(39.6%),91 cases were New type H1N1(19.3%),82 cases were B type(17.4%)and 81 cases were Yamagata type(17.2%).Conclusion From 2010 to 2014,the influenza was more active in the autumn and winter seasons in Huainan,and steady in other seasons.The H3 type is the main season separation plants.