Expression of interleukin-17A in asthmatic mice and its significance / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the serum level of interleukin-17A (IL-17A) and its expressions in the lung, spleen and thymus in asthmatic mice.</p><p><b>METHODS</b>In 14 normal BALB/c female mice and 14 asthmatic mice, the changes in the airway pathology and the cell proportion in the bronchoalveolar lavage fluid (BALF) were observed. The serum level of IL-17A and IL-17A expressions in the tissue homogenates of the lung, spleen and thymus of the mice were detected by sandwich enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The airway inflammation in the asthmatic mice was characterized mainly by eosinophil and neutrophil infiltration, which was not observed in the normal control group. Serum IL-17A levels and IL-17A expressions in the lung, spleen and thymus of the asthmatic mice were significantly higher than those in the normal control group (P<0.01). In the asthmatic mice, IL-17A expression in the lung tissues was positively correlated with the percentages of neutrophils (r=0.693, P=0.040) and eosinophils (r=0.733, P=0.030) in the BALF.</p><p><b>CONCLUSION</b>IL-17A is highly expressed in the serum, lung, spleen and thymus of asthmatic mice. IL-17A may be one of the major cytokines involved in exacerbation of bronchial asthma, and is probably associated with the recruitment of neutrophils and eosinophils into the airways.</p>

Study on spaceflight mutagenesis Platycodon grandiflorum via FTIR / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a method for analyzing amorphous organic compound components of space Platycodon grandflorum rapidly.</p><p><b>METHOD</b>FTIR was applied for measuring and comparing P. grandflorum of comparison group,the ground group and the 4" generation of space group.</p><p><b>RESULT</b>Several active components (platycodin, polysaccharide etc.) contents of space group are increased obviously.</p><p><b>CONCLUSION</b>The major components and the structures remained inextenso,and the effictive component contents are enhanced in the space P. grandiflorum. FTIR is a fast method to analyze the changes of amorphous organic compound components of the space traditional Chinese medicines.</p>