Comparative proteomics study of sensory and motor fascicles of peripheral nerve / 中风与神经疾病杂志

@#Objective　It is important to distinguish between motor and sensory fascicles of the peripheral nerves for nerve alignment in surgery.No biomarkers currently are available for identification of motor or sensory fascicles.The objective of this study is to search the specific proteins between sensory and motor fascicles of peripheral nerves and provide biomarkers for the identification of functional fascicles of peripheral nerves.Methods　The normal state of motor branch and saphenous nerve of femoral nerve in Wistar rats,and at 8 hours and 8 days after Sunderland V injury were respectively sampled.Five mm long samples were taken from the distal side of the broken end,and a total of 18 groups of proteins were isolated from 6 samples.After purification and quantification,differential gel electrophoresis (DIGE) was used to label the proteins,gel image was scanned,and image analysis software (DeCyder) was used to compare and identify the differentially expressed proteins in each group.Protein spots with more than 1.5 times of difference in expression were selected to prepare glue-cutting,enzyme-cutting and spot target.PMF chromatogram was analyzed and identified by MALDI-TOF-PRO mass spectrometer,and the results of proteomics were analyzed and compared by RT-PCR.Chi-square tests and t-tests were performed for comparison between motor or sensory nerve groups.Results　The data identified 6 proteins that were differentially expressed between motor and sensory fascicles (>1.5-fold,P<0.05),including Annexin V,neurofilament light polypepticle,TEC kinase,serine protease inhibitor A3N,Peroxiredoxin-2,and TPM1.The proteomic results were consistent with the mRNA expression levels of these genes as determined by quantitative reverse transcription polymerase chain reaction.Conclusion　There were significant differences in proteomic expression between the peripheral sensory and motor fascicles,and Annexin V can be used as a high-difference marker protein to distinguish the peripheral sensory from motor fascicles.

FIBER PROJECTIONS FROM THE NUCLEI OF THE TRIGEMINAL NERVE TO THE CEREBELLAR CORTEX OF THE RAT——A STUDY WITH THE HRP METHOD / 解剖学报

The trigemino-cerebellar projections of rats were studied by introducing HRP microelectrophoretically into various areas of the cerebellar cortex. The results indicate that the following parts of the cerebellum receive bilateral (mostly ipsilateral) trigeminal projections, namely, the simple lobule, the crusa Ⅰ and Ⅱ, the paramedian lobuIe, the dorsal paraflocculus, the lateral part of the lobule Ⅷ and the vermal cortex of the lobules Ⅵ～Ⅸ.Fibers from the interpolar subnucleus and the principal sensory nucleus of the trigeminal nerve project to all of the above mentioned areas.The caudal subnucleus projects to the crus Ⅰ, the paramedian lobule, the dorsal paraflocculus, the lateral part of the lobule Ⅷ and the vermal cortex of the lobules Ⅵ～Ⅸ.The oral subnucleus gives its projections to the crus Ⅱ, the paramedian lobule, the lateral part of the lobule Ⅷ and the vermal cortex of the lobules Ⅶ～Ⅸ.The mesencephalic nucleus of the trigeminal nerve sends fibers to the crura Ⅰ and Ⅱ, the paramedian lobule, the lateral part of the lobule Ⅷ and the vermal cortex of lobules Ⅶ～Ⅸ.A few labeled neurons were found in the motor nucleus of the trigeminal nerve; while in the region ventro-lateral to the motor nucleus, in the root of the trigeminal nerve and in areas adjacent to it large amount of labeled cells were seen in all the cases studied.Unexpectedly, several labeled neurons were seen in a semilunar ganglion of the trigeminal nerve.

LOCALIZATION OF THE CELL BODIES OF THE PHRENIC MOTOR AND SENSORY NEURONES IN RABBIT BY HRP METHOD / 解剖学报

Eight rabbits were used in this study.The position of the phrenic nucleus in thespinal cord,the morphology of the phrenic motoneurones and position of the cellbodies of the sensory neurons of the phrenic nerve were determined by using themethod of HRP labelling through the centralcutting end of the left phrenic nerve atthe root of the neck.The results were as follows:1.The phrenic nucleus in the rabbit was located in C_3,C_4,and C_5 segments.Itis a longitudinal cell column lying between the ventromedial and the ventrolateralcolumns of the ventral horn of the spinal cord.2.Phrenic motoneurones differed in shape and size.Most of the cell bodies ofthe rabbit's phrenic motoneurones were round or oval in shape,ranging from 5 to45 ?m(mean 25 ?m)in diameter.3.The rabbit phrenic nerve arises from the ventral rami of the 3 rd,4 th and5 th cervical nerves,and the nucleus of this nerve does not extend beyond the 3 rd-5 th segments——the location of the nucleus corresponds with the segmental rootsfrom which the phrenic nerve arises.4.The cell bodies of the sensory neurones of the rabbit's phrenic nerve werelocated in the dorsal root ganglia of the third and fourth cervical nerves.Besides,50 rabbits were dissected,and the origin of their phrenic nerves werestudied.