5HT2A modulation attenuates pancreatic cancer induced pain mouse model by inhibiting HDAC

Purpose Patients have been severely suffered from cancer associated pain, and pancreatic cancer is the most severe form of cancer associated with pain. There are very few options available to manage it. The present report evaluated the effect of 5HT2A on pancreatic cancer associated pain. Methods Pancreatic cancer was induced by injecting SW 1,990 cells (~3×106 in a 20 µL suspension) into the pancreas and formed a 2­3-mm vesicle using an inoculator fitted with a 26-gauge needle in BALB/c-nu mice. Survival rate and body weight of the mice were observed. Pain behaviour testing was performed at the end of each week (third and fourth week) after surgery. Inflammatory mediators and HDAC 2 proteins were determined in the spinal tissue using quantitative real-time polymerase chain reaction. Results There was improvement in the survival rate and body weight in 5HT2A antagonist treated group than pancreatic cancer group of mice. Moreover, 5HT2A antagonist ameliorated the alteration in pain behaviour of pancreatic cancer mice. mRNA expression of HDAC2 and level of inflammatory cytokines were reduced in the spinal tissue of 5HT 2A antagonist treated group than pancreatic cancer group of mice. Conclusions Data revealed that 5HT2A antagonist ameliorates pain associated with pancreatic cancer mice by HDAC inhibition and inflammatory cytokines. The result of investigation supports that modulation of 5HT2A receptor could be used clinically to protects neuropathic pain in pancreatic cancer.

Influence of physical exercise on mental health of college students during the epidemic of COVID-19 / 中国学校卫生

Objective@#To investigate the effect of physical exercise on mental health of college students during the epidemic of COVID-19, and to provide reference for mental health education in special period.@*Methods@#By using basic demographic data questionnaire, physical exercise questionnaire, the Generalized Anxiety Disorder 7(GAD-7) and Patient Health Questionnaire(PHQ-9), an internet-based questionnaire survey was conducted among 1 297 college students in Fuzhou. Pearson correlation analysis and a multivariate Logistic regression model were used for statistical analysis.@*Results@#Among the college students, 58.7% took part in physical exercise more than or equal to three times a week, 27.8% of them took part in physical exercise more than or equal to 30 minutes each time, 55.4% of them had took part in moderate-intensity activity each time, only 16.6% of them reported regular exercise. The overall detection rates of anxiety were 20.0%, with mild, moderate and severe anxiety being 16.4%, 2.3% and 1.2%, respectively. The detection rates of depression were 24.7%, with mild, moderate, moderate-to-severe and severe depression being 18.0%, 4.4%, 1.2% and 1.1%, respectively. The analysis results of multivariate Logistic regression show that college students who had no exercise at present and have no intention of regular exercise in the next 2 months(OR=3.36) and have no exercise at present but plan to start regular exercise in the next 2 months(OR=4.07) or those who exercise occasionally(OR=2.65) are prone to develop mild anxiety(P<0.05). College students who had no exercise at present and have no intention of regular exercise in the next 2 months(OR=5.66, 3.86) were prone to develop mild and moderate depression(P<0.05).@*Conclusion@#College students reported certain degree of anxiety and depression during the epidemic of COVID-19. Anxiety and depression are closely related. Participation in physical exercise is associated with their mental health among college students. Universities, families and relevant departments should take measures to promote college students to participate in moderate and regular physical exercise.