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Objective:The targets and signaling pathways of Xuanfei Huazhuo prescription (XFHZP) for the treatment of coronavirus disease-2019 (COVID-19) were explored, and its possible action mechanisms were described through network pharmacology and basic analysis of modern pharmacology. Method:The compounds and targets in XFHZP were collected through TCMSP and BATMAN-TCM databases. The targets of COVID-19 were studied by GeneCards, NCBI and CTD databases. The PPI network was constructed through STRING database. The networks of "herb-meridian" and "traditional Chinese medicines-compounds-targets-disease" were generated by Cytoscape 3.7.0. Then, Kyoto Encyclopedia of Genes and Genomes（KEGG） analysis and Gene Ontology（GO） analysis were made for shared targets through the Omicshare platform. In addition, the disease targets of multiple organ injury, immune injury and severe acute respiratory syndrome (SARS) were retrieved and then mapped with XFHZP. The ratio of intersection targets to XFHZP's targets was calculated. Result:XFHZP has 10 traditional Chinese medicines in total, including 6 medicines with the meridian tropism to lung, 5 medicines with the meridian tropism to the spleen and 5 medicines with the meridian tropism to the stomach. There were 409 compounds and 2 271 targets. There were 8 same inflammatory factors in targets between XFHZP and COVID-19, and each inflammatory factor corresponded to multiple compounds. XFHZP and COVID-19 had 135 intersection targets, and 36 key targets were screened out. A total of 172 signaling pathways were screened out through KEGG signal pathway enrichment (P<0.05). There were 4 000 biological processes, 254 cell components, and 408 molecular functions (P<0.05) according to GO analysis. XFHZP had many common targets with various organ damage targets and immune damage targets, with the ratio of about 7.6%-97.8%. XFHZP had 173 intersection targets with SARS. Conclusion:XFHZP may treat COVID-19 through anti-inflammatory, organ protecting and immune effects. It will provide a certain theoretical basis for the development of drugs for COVID-19.
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Objective To assess the diagnostic and prognostic value of measuring presepsin in patients with acute respiratory distress syndrome (ARDS).Methods Plasma prsepsin was collected from 81 patients with ARDS,27 patients with cardiogenic pulmonary edema (CPE) and 20 healthy volunteers at enrollment.Levels of presepsin were measured using the PATHFAST(R) analysis system based on a chemiluminescent enzyme immunoassay (CLEIA).The differences of plasma prsepsin were compared between different groups.The 28-day mortality were followed in ARDS patients,and the characteristics of the surviors and non-surviors were compared.Results ARDS patients had significantly higher median levels of presepsin compared to CPE patients [926.89 (485.41-2 662.32)pg/mL vs.376.21 (247.16-568.52) pg/mL,P＜0.001] at enrollment.The difference between infected and non-infected ARDS patients did not showed statistical significance [(934.74 (456.44-3 322.51) pg/mL vs.798.12 (485.41-2 561.40) pg/mL,P--0.079).In ARDS patients,the presepsin levels of non-survivors was significantly higher than that of survivors [3 158.3 (963.91-4 489.33) pg/mL vs.729.09 (398.05-1 467.24) pg/mL,P＜0.001],and multivariate Logistic regression showed that presepsin (OR =1.51,P =0.027) was the independent predictor for 28-day mortality in ARDS patients with acute lung injury (ALI).Conclusions Presepsin was an effective indicator in diagnosing ARDS,and it also was a strong prognostic marker for short-term mortality in ARDS.
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Objectivc To compare the accuracy and practicability of OPD-Scan Ⅲ marker methods and slit light narrow band marker methods in phacoemulsification and Toric IOL implantation.Methods A prospective randomized controlled study was conducted in 70 eyes of 70 patients with regular astigmatism (1.00-3.00 D).All patients underwent phacoemulsification and Toric IOL implantation and were randomly divided into two groups according to preoperative marker methods:observant group,in which OPD-Scan Ⅲ marker methods were used in 35 eyes of 35 patients,and control group,with application of slit light narrow band marker methods in 35 eyes of 35 patients.The postoperative visual acuity,residual astigmatism and deviation of lens axis (LAD) were observed and compared between the two groups.Results The uncorrected visual acuity (UCVA) between preoperative data and 3 months after surgery was statistically significant [(0.11 ± 0.09) vs.(0.59 ± 0.25)] (P =0.000).The difference in the postoperative 1 day and 3 months LAD was no statistically significant (P ＞ 0.05).The astigmatism between preoperation and 3 months after treatment was significant [(1.88 ± 0.49) D vs.(0.54 ± 0.30) D] (P =0.000).There was no significant difference in LAD between the two groups neither the first day nor 3 months after operation (both P =0.621).Conclusion OPD-Scan Ⅲ marker methods and slit light narrow band marker methods are both highly accurate and practical,but the former requires less for operator and is more easily accepted by patients.
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Objective To investigate the diagnostic and prognostic value of plasma soluble CD163 (sCD163) levels in patients with acute respiratory distress syndrome (ARDS).Methods A total of 124 individuals,83 patients with ARDS,20 patients with cardiogenic pulmonary edema (CPE),and 21 healthy controls,were enrolled in this study.ARDS patients were classified into survivors and nonsurvivors according to 28-day mortality.The concentration of plasma sCD163 was measured by enzymelinked immunosorbent assay (ELISA).Receiver operating characteristic (ROC) curves were employed to evaluate the accuracy of sCD163 in diagnosing ARDS and predicting 28-day ICU outcome.Cumulative survival curve was carried out by Kaplan-Meier survival analysis.Logistic regression analysis was assessed by univariate and multivariate analysis to identify independent predictors of outcome controlling for reported risk factor of mortality.Results Patients with ARDS had significantly higher median levels of sCD163 compared to patients with CPE [496.7(421.8-577.5) ng/mL vs.284.5(141.7-459.2) ng/mL,P＜0.001] upon admission to ICU.The sCD163 levels of non-survivors was significantly higher than that of survivors [577.5 (503.7-623.4) ng/mL vs.479.6 (395.4-520.8) ng/mL,P＜0.001].Multivariate logistic regression showed sCD163 (OR =1.02,P =0.001)was the independent predictor for 28-day mortality in patients with ARDS.Conclusions Plasma sCD163 is a potential biomarker for diagnosis of ARDS and differenting the severity of ARDS.Meanwhile,sCD163 was an independent prognostic marker for 28-day mortality in ARDS patients.
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Background Instability of tear film,increase of ocular surface temperature and tear evaporation are the associated factors of both bubbles and dry eye.But the relationship between dry eye and ocular surface bubbles is unclear.The bubbles are easily retained and checked in the inferior conjunctiva fornix.Objective This study aimed to study the relationship between dry eye and bubbles in the inferior conjunctiva fornix.Methods Sixty-four eyes of 32 patients with binocular irritative symptoms and bubbles or without bubbles in the inferior conjunctiva fornix were collected in Affiliated Tianyou Hospital of Wuhan University of Science and Technology from January to May,2012.The bubbles in the inferior conjunctiva fornix were examined by slit lamp microscope,and the break-up time (BUT) and Schirmer Ⅰ test (S Ⅰ t) were performed.The differences of BUT and S Ⅰ t between the two groups were compared using independent sample t test,and abnormality rate of examination indexes was statistical analyzed by CHI-Square test using a SPSS 11.5 software.Written informed consent was obtained prior to the clinical survey.Results In the 64 eyes of the bubbles group,incidence of dry eye was 75.00％,and the incidence of both BUT＜5 seconds and S Ⅰ t＜5 mm/5 minutes was 46.88％ ;while the incidence of BUT＜5 seconds or S Ⅰ t＜5 mm/5 minutes was 53.13％ and 57.81％,respectively.In 64 eyes without bubbles,incidence of dry eye was 31.25％,the incidence of both BUT＜5 seconds and S Ⅰ t＜5 mm/5 minutes was 18.75％,that of BUT＜5 seconds or S Ⅰ t＜5 mm/5 minutes was 21.88％ and 20.31％,respectively.The incidences of dry eye,both BUT＜5 seconds and S Ⅰ t＜5 mm/5 minutes,BUT ＜5 seconds,S Ⅰ t＜5 mm/5 minutes were significantly higher in the bubbles group than those of the without bubbles group (all at P=0.00).In the 68 eyes determined as dry eyes,48 eyes (48/68) were in the bubbles group and 20eyes (20/68) were in the without bubbles group,showing a significant difference between the two groups (x2 =24.60,P＜0.01).The incidences of both BUT＜5 seconds,S Ⅰ t＜5 mm/5 minutes were not significantly different between the two groups (x2 =0.01,P=0.95;x2 =1.06,P=0.30).Conclusions The bubbles in the inferior conjunctiva fornix is highly correlated with dry eye.
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<p><b>OBJECTIVE</b>To study the relationship between enhanced degree in the cancer lesion and angiogenesis in the tumor through early manifestations in enhanced helical CT in patients with bladder cancer.</p><p><b>METHODS</b>Fifty-three patients with bladder carcinoma were examined by pelvic plain CT and helical CT scan at peak enhancement of cancer lesion. Histologic grade, vascular endothelial growth factor (VEGF) and micro-vascular density (MVD) were analyzed for each resected cancer lesion. Pearson and Spearman correlation tests were used to assess the relationship between CT enhancement and histologic grade, VEGF or MVD.</p><p><b>RESULTS</b>In early enhancing phase of helical CT, different degrees of enhancement were observed in 73 bladder cancer lesions. The difference between average CT attenuation and MVD in different histologic grade cancer lesions was statistically significant (P < 0.001). A positive correlation was found between the CT enhancement and MVD (gamma = 0.936, P < 0.001), histologic grade (gamma = 0.75, P < 0.001), but VEGF of bladder cancer did not correlate with the CT enhancement or MVD.</p><p><b>CONCLUSION</b>The early enhancement of helical CT enhancement of bladder cancer, showing a positive relation to MVD and histologic grade, can reflect the tumor angiogenesis and blood supply.</p>