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This study aims to mine the transcription factors that affect the genuineness of Codonopsis pilosula in Shanxi based on the transcriptome data of C. pilosula samples collected from Shanxi and Gansu, and then analyze the gene expression patterns, which will provide a theoretical basis for the molecular assisted breeding of C. pilosula. Gene ontology(GO) functional annotation, conserved motif prediction, and gene expression pattern analysis were performed for the differential transcription factors predicted based on the transcriptome data of C. pilosula from different habitats. A total of 61 differentially expressed genes(DEGs) were screened out from the transcriptome data. Most of the DEGs belonged to AP2/ERF-ERF family, with the conserved motif of [2X]-[LG]-[3X]-T-[3X]-[AARAYDRAA]-[3X]-[RG]-[2X]-A-[2X]-[NFP]. Forty-three of the DEGs showed significantly higher gene expression in C. pilosula samples from Shanxi than in the samples from Gansu, including 11 genes in the AP2/ERF-ERF family, 5 genes in the NAC fa-mily, 1 gene in the bHLH family, and 2 genes in the RWP-RK family, while 18 transcription factors showed higher expression levels in the samples from Gansu. GO annotation predicted that most of the DEGs were enriched in GO terms related to transcriptional binding activity(103), metabolic process(26), and stress response(23). The expression of transcription factor genes, CpNAC92, CpNAC100, CpbHLH128, and CpRAP2-7 was higher in the samples from Shanxi and in the roots of C. pilosula. CpNAC92, CpbHLH128, and CpRAP2-7 responded to the low temperature, temperature difference, and iron stresses, while CpNAC100 only responded to low temperature and iron stresses. The screening and expression analysis of the specific transcription factors CpNAC92, CpNAC100, CpbHLH128, and CpRAP2-7 in C. pilosula in Shanxi laid a theoretical foundation for further research on the mechanism of genuineness formation of C. pilosula.
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Codonopsis/chemistry , Transcription Factors/genetics , Gene Expression Profiling , Transcriptome , IronРеферат
Objective:To investigate the common cause of spinal surgery in ankylosing spondylitis (AS) and to develop reasonable and effective treatment programs for rhematologists.Methods:In this study,79 AS patients (72 males,7 females) hospitalized in the Department of Orthopedic and received spinal surgery in Peking University Third Hospital from January 2007 to July 2013 were investigated retrospectively.The causes of the spinal surgery were analyzed.Results:The majority of the surgery were of cervical vertebra surgery for 59 cases (74.7%),followed by the thoracolumbar vertebra,thoracic vertebra,cervicothoracic vertebra,and lumbar vertebra.The common causes of AS spinal surgery were instability (53.2%) including fracture and dislocation caused by trauma mostly,followed by compression symptoms (34.1%).However,the orthopaedic surgery (12.7%) was not the predominant surgery causes.The most common cause of cervical surgery was cervical spondylosis (25 cases),followed by cervical fracture (22 cases) and cervical dislocation (10 cases).For cervical fracture,the most common site was C7 (8 cases).Atlanto-axial vertebral lesions (13 cases) induced by dislocation,subluxation,instability and fracture were common in cervical vertebra surgery.The age and disease duration of atlantoaxial surgical patient's were less than other parts of the cervical surgery.Thoracolumbar vertebra surgery included thoracolumbar kyphosis orthopaedic surgery (10 cases),and fractures (6 cases),which mainly occurred in T11-12 vertebrae.According to the time of admission for surgery,the cases of AS that received spinal surgery were 34 from 2007 to 2008.The number of the cases that accepted the surgery decreased gradually year by year.Conclusion:The majority surgery section was cervical vertebra,and atlanto-axial vertebral lesions were not rare as we thought.The main etiology of spinal surgery for AS patients was instability,e.g.fracture and compression symptoms.Of course,trauma accounted for the relative percentage of this phetonomenon.Although,the annual number of patients who receives spinal surgery is decreasing,to go to make early diagnosis and treatment,and to standardize patient education for AS patients are still important.
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Objective To explore the association between different visual display terminal use and physical symptoms among adults.Methods Hangzhou,Jiaxing,Huzhou,Jinhua and Quzhou City were selected,where residents aged 18 years old and above were investigated using Mitofsky-Waksberg two-stage sampling.Information of demography,different visual display terminal use,eye and musculoskeletal symptoms,eye disease and eye relax were collected.Backward stepwise logistic regression was used to explore the association between different visual display terminal use and the symptoms (P value for addition < 0.05,P value for removal > 0.1).Results Totally 350 adults responded to the investigation,with a response rate of 48.54%.Finally,333 respondents were included in the analysis,among which 120 reported eye symptoms (36.04%) and 71reported musculoskeletal symptoms (21.32%).More time of computer use at work(0.5-4 h:OR=2.006,95% CI:1.021-3.943;4-6 h:OR=3.578,95% CI:1.751-7.315;>6 h:OR=4.874,95% CI:1.897-12.521) and eye disease (OR =4.361,95% CI:2.465-7.714) were significandy associated with higher odds ratios in eye symptoms.Similar pattern was seen for the association on time of computer use at work with musculoskeletal symptoms (0.5-4 h:OR =3.815,95 % CI:1.809-8.045;4-6 h:OR =4.974,95 % CI:2.281-10.845;> 6 h:OR =5.934,95% CI:2.347-15.005).More time of watching TV on television (> 2 h:OR =2.051,95%CI:1.068-3.939) and women (OR =1.884,95% CI:1.052-3.372) were also observed to be statistically associated with musculoskeletal symptoms.However,no statistical association was observed between the two symptoms and entertainment purpose use of computer/cell phone/pad/kindle (P > 0.05).Conclusion Computer use at work was significantly associated with eye symptoms,especially for those having eye diseases.It was also statistically associated with musculoskeletal symptoms,especially for women.
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Objective To explore the relationship between pulse blood pressure and cognitive function for the elders in Yuhuan County,Zhejiang Province.Methods Multi -stage cluster method was used to select 1 700 residents aged over 60 years in Yuhuan County,Zhejiang Province.Mini -Mental State Examination (MMSE)was used to screen cognitive impairment.Locally weighted scatter plot smoothing was used to explore the local linear trend between MMSE score and diastolic.The association between pulse pressure or blood pressure and cognitive function was analyzed by multivariate logistic regression analysis.Results The prevalence of cognitive impairments for the elders aged over 60 years in Yuhuan County was 15.74%.There was significant difference between the pulse blood pressure of cognitive impairment group (64.01 ±16.31)mmHg and none -cognitive impairment group (58.97 ±14.67)mmHg.The t value was 4.97.Pulse blood pressure and the elderly MMSE score showed a significant positive correlation.Multivariate logistic regression analysis showed that cognitive function related to diastolic blood pressure (OR =1.010,95% CI:1.001 -1.019,P =0.034)after adjusting several covariate.Conclusion Normal cognitive function was associated with decreased pulse blood pressure among the elders aged over 60 years in Yunhuan County,Zhejiang Province.
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<p><b>OBJECTIVE</b>To investigate the association between low-density lipoprotein receptor-related protein 5 (LRP5) variants (rs12363572 and rs4930588) and type 2 diabetes mellitus (T2DM) in Han Chinese.</p><p><b>METHODS</b>A total of 1842 T2DM cases (507 newly diagnosed cases and 1335 previously diagnosed cases) and 7777 controls were included in this case-control study. PCR-RFLP was conducted to detect the genotype of the two single nucleotide polymorphisms (SNPs). Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated to describe the strength of the association by logistic regression.</p><p><b>RESULTS</b>In the study subjects, neither rs12363572 nor rs4930588 was significantly associated with T2DM, even after adjusting for relevant covariates. When stratified by body mass index (BMI), the two SNPs were also not associated with T2DM. Among the 3 common haplotypes, only haplotype TT was associated with reduced risk of T2DM (OR 0.820, 95% CI 0.732-0.919). In addition, rs12363572 was associated with BMI (P<0.001) and rs4930588 was associated with triglyceride levels (P=0.043) in 507 newly diagnosed T2DM cases but not in healthy controls.</p><p><b>CONCLUSION</b>No LRP5 variant was found to be associated with T2DM in Han Chinese, but haplotype TT was found to be associated with T2DM.</p>
Тема - темы
Female , Humans , Male , Middle Aged , Asian People , Genetics , Body Mass Index , Case-Control Studies , Diabetes Mellitus, Type 2 , Blood , Genetics , Haplotypes , Logistic Models , Low Density Lipoprotein Receptor-Related Protein-5 , Genetics , Odds Ratio , Polymorphism, Single Nucleotide , Rural Population , Triglycerides , BloodРеферат
<p><b>OBJECTIVE</b>To explore the effect of N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide(W7) on the differentiation from human adipose-derived mesenchymal stem cells (hADSCs) to endothelial cells.</p><p><b>METHODS</b>hADSCs were cultured with serum-free differential medium containing 40 ng/ml vascular endothelial growth factor (VEGF) and 10ng/ml basic fibroblast growth factor (bFGF). Cells were divided into control group (differential medium without W7), high-dose group (containing 30 μmol/L W7), medium-dose group (containing 20 μmol/L W7), and low-dose group ( containing 10 μmol/L W7). The hADSCs were cultured for 8 days, and then the changes in the phenotypes of von Willebrand factor (vWF) and vessel-selective cadherin (VE-Cadherin) were detected by flow cytometry (FCM). The intracellular Ca(2+) labeled with Fluo-3 was detected by laser confocal microscopy. After hADSCs planting on Matrigel, their angiogenic potentials were observed under the inverted phase contrast microscope, and the expression of extracellular regulated kinase (ERK) and phosphorylated extracellular regulated kinase (p-ERK) were evaluated by Western blot.</p><p><b>RESULTS</b>After the hADSCs were cultured for 8 days, compared with the control group, the expressions of vWF and VE-Cadherin significantly increased along with the decrease of W7 level and the intracellular Ca(2+) also significantly increased (Pü0.01). Lumina-like vascular structure was formed in W7 treatment groups, but not in the blank control group. Compared with the blank control group, the expression of ERK showed no significant in W7 treatment groups (high-, medium-, and low-dose groups)(P>0.05); however, along with the decrease of W7 levels, the expression of p-ERK significantly increased(P<0.05).</p><p><b>CONCLUSION</b>W7 in proper levels can effectively induce the differentiation from hADSCs to endothelium by increasing the intracellular Ca(2+) level and thus activating the ERK/MAPK pathway.</p>
Тема - темы
Humans , Adipose Tissue , Cell Biology , Cell Differentiation , Cells, Cultured , Endothelial Cells , Cell Biology , Metabolism , Mesenchymal Stem Cells , Cell Biology , Metabolism , Sulfonamides , PharmacologyРеферат
Objective To study the effect of granulocyte-macrophage colony-stimulating factor(GM-CSF)on angiogenesis of rat with acute myocardial injury induced by isoproterenol(Iso). Methods A total of 60 adult male Wistar rats were randomly divided into 3 groups: normal control group, GM-CSF pretreatment group (GM-CSF group), and lso injury group, 20 rats in each group. GM-CSF group was administered recombinant human(rh)GM-CSF(5.0 μg/kg), through tail intravenous injection once a day for three days. Then the GM-CSF group and the Iso injury group were anesthetized by intraperitoneal injection of lso( 15.0 mg/kg) once a day for three days. The same dose of saline was administered in the same way to the control rats. Ten days after injection, pathological changes of myocardial damage and infarct area were examined by immunohistochemistry. The mRNA expression levels of polypeptide antigen (CD34), vascular endothelial growth factor (VEGF) and its receptor KDR/flk- 1 were measured by RT-PCR. Results The difference of myocardial necrosis area between groups was statistically significant(F=10.07, P < 0.01), in which GM-CSF group[(37.37 ± 12.98)%] was significantly less than Iso injury group[(45.51 ±14.96)%, P < 0.05]. The difference of myocardial neovascularization density index of rats between groups was statistically significant ( F = 25.54, P < 0.05 ), in which GM-CSF group [(3980.05 ± 477.22) No/mm2] was significantly higher than Iso injury group((2605.93±361.49)No/mm2,P<0.01).The differences of myocardial CD34,VEGF,KDR/flk-1 mRNA expression between groups were statistically significant(F=17.83,4.29,4.10,all P<0.01).Compared to Iso mjury group[CD34(23.85±6.06),VEGF(31.80±8.05),KDR/flk-1(30.16±8.01)]were higher in the GM-CSF group[CD34(44.04±10.13),VEGF(49A±11.59),and KDR/flk-1(46A9±7.90),all P<0.01].The expressions of myocardiM VEGF mRNA and its receptor KDR/flk-1 mRNA was positively correlated(r=0.725,R2=0.526,P<0.01).Conclusions GM-CSF prelreatmcnt increases the density ofnew blood vessels in myocardium,and reduces the Iso-induced myocardial injury in rats.
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Objective: To observe the expression of CD133 in the clear cell renal cell carcinoma (RCC) and to investigate the related drug resistance. Methods: Flow cytometry and immunohistochemical method were used to examine the expression of CD133 in metastatic RCC cell line RCC05-TXJ, low metastatic RCC cell line RCC05-ZYJ and two clinical non-metastatic RCC primary cultures isolated from a male and a female patient. The four cell lines were treated with IFN-α and 5-FU and the viability of cells were examined by MTT assay before and after treatment. Results: Flow cytometry showed that RCC05-TXJ and RCC05-ZYJ expressed CD133. The in situ carcinomas of male and female patients hardly expressed CD133. Immunohistochemistry examination showed that the local membrane of RCC05-TXJ and RCC05-ZYJ cells expressed CD133. RCC cells of female and male patients hardly expressed CD133. RCC05-TXJ and RCC05-ZYJ cells exposed to IFN-α or 5-FU showed a rebound of survival 24 h after withdrawal of drugs. The survival rates of RCC cells of male and female patients kept at a low level after withdrawal of drugs. Conclusion: There are a small number of CD133+ RCC cells in RCC patients, with a property similar to tumor stem cells, which might be one of the important factors influencing the immunotherapy and chemotherapy of tumors.
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Objective: To establish clear cell renal cell carcinoma (ccRCC) cell lines from clinical ccRCC specimens of Han nationality in China and to characterize the biological features. Methods: From 2005 to 2007, fresh surgical samples of ccRCC were obtained from 43 patients; the samples included primary tumor in situ, osseous metastasis, lymph node metastasis, and cancerous embolus. The samples were cultured in vitro using explant-culture method within 30-60 min after surgery. Analysis on cell growth and colony-forming efficiency was recorded for the lines which were passaged for over 50 generations. Chromosome examination, pathological examination and tumorigenesis in NOD-SCID mice were used to determine their malignancy. Flow cytometry was used to determine expression of CA9 and CD133. Results: Most of the primary cells could only be passaged for less than 5 generations; 5 lines could be serially passaged for over 5 passages, 3 lines for over 10 passages, and only 2 lines could be stably passaged. One line,named RCC05-TXJ, was from osseous metastatic ccRCC and had been serially passaged for 110 generations in 21 months; the average doubling time was 19.2 h,average chromosome number was 75,and colony forming efficiency was 41%. Another line,named RCC05-ZYJ,was from primary ccRCC specimen and had been serially passaged for 160 generations in 18 months; the average doubling time was 16.5 h,average chromosome number was 55,and the colony forming efficiency was 37%. Immunohistological analysis demonstrated that both lines expressed CA9 and CD133. Flow cytometry analysis found that expression levels of CA9 and CD133 increased with the passages. Both RCC05-ZYJ and RCC05-TXJ lines were able to form tumor and to metastasize in NOD-SCID mice; however, their metastatic ability was obviously different. Conclusion: We have established 2 ccRCC cell lines with different metastatic potentials from the clinical ccRCC specimens of Han nationality in China. The ratio of tumor stem cells increases with the passages.