Comparison of autologous serum and deproteinised calf serum eye gel in the treatment of rabbit corneal alkali burn / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To compare the effect of using autologous serum and deproteinised calf serum eye gel in the treatment of corneal alkali burn through establishing corneal alkali burn models. <p>METHODS: Alkali burn model of cornea was established on the right eyes by putting the filter paper with 1.0mol/L NaOH on the center cornea for 1min in 30 white rabbits. The model rabbits were divided randomly into 3 groups after scoring based on Hughes criteria. Normal saline, calf blood deproteinized eye gel and autologous serum eye drops 4 times/day, atropine eye gel 1 times/night, ofloxacin eye gel 1 times/night for 2wk respectively. The morphology of corneal neovascularization was observed on the 7 and 14d, and the area was calculated. On the 14d, the corneas of each group were removed and routine histopathological examinations were performed according to the groups. The concentration of CD45, IL-10, IFN-γ and VEGF in corneal homogenate were determined. <p>RESULTS:Area of corneal neovascularization: on the 7 and 14d, the area of corneal neovascularization of Group DCS(29.48±2.27, 34.19±2.67mm2), AS(34.19±2.67, 33.89±2.74mm2)(<i>P</i>>0.05). Concentration of CD45, IL-10, IFN-γ, VEGF in cornea homogenate(pg/mL): on the 14th day, the concentration of CD45 Group DCS(0.56±0.04ng/mL), AS(0.54±0.05ng/mL)<Group Ctrl(1.27±0.07ng/mL)(<i>P</i><0.05). The concentration of IL-10 Group AS(452.49±11.40pg/mL)>Group DCS>(332.49±13.67pg/mL)>Group Ctrl(111.05±6.95pg/mL)(<i>P</i><0.05). The Concentration of IFN-γ Group DCS(23.20±2.89pg/mL), AS(22.61±2.72pg/mL)<Group Ctrl(41.77±4.26pg/mL). They have a significant difference(<i>P</i><0.05). The concentration of VEGF Group DCS(151.14±18.21pg/mL), AS(149.11±14.75pg/mL)<Group Ctrl(391.35±28.59pg/mL)(<i>P</i><0.05). <p>CONCLUSION:AS has the same effect as DCS in inhibiting the release of inflammatory factors(CD45, IFN-gamma and VEGF)and the formation of corneal neovascularization after alkali burn in rabbits, and AS has the strongest effect in promoting the release of anti-inflammatory factors(IL-10)and inhibiting the infiltration of inflammatory cells, followed by DCS.

Clinical observation of vitamin A Palmitate Eye Gel combined with Tobramycin Eye Drops in repairing of corneal epithelial defect / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the clinical effect of vitamin A Palmitate Eye Gel combined with Tobramycin Eye Drops on corneal epithelial injury.<p>METHODS: Totally 80 patients(100 eyes)with corneal epithelial injury who came to our hospital from May 2015 to March 2016 were randomly divided into treatment group(50 eyes)and control group(50 eyes). The treatment group was treated with vitamin A Palmitate Eye Gel combined with Tobramycin Eye Drops, while the control group was treated with Tobramycin Eye Drops only. The curative effect of two groups was compared after 2wk and 4wk of treatment.<p>RESULTS: The cure rate was 80% and the effective rate was 94% in the treatment group. The cure rate was 70% and the effective rate was 78% in the control group. There was significant difference in the effective rate between the two groups(<i>P</i><0.05). The results of SⅠt and BUT test showed that the time of treatment group was significantly longer than that of control group(<i>P<</i>0.01). The score of symptoms and signs in the treatment group was significantly lower than that in the control group after 2wk of treatment(<i>P<</i>0.01), but there was no significant between treatment groups and control grouop after 4wk of treatment(<i>P></i>0.05).<p>CONCLUSION: Vitamin A Palmitate Eye Gel combined with Tobramycin Eye Drops can significantly improve dry eye symptoms and effectively shorten the healing time of corneal epithelium. It is an effective method to treat corneal epithelial injury.

Qiming Granules combined with Deproteinized Calfblood Extract Eye Gel in the treatment of diabetes phaeoemulsification postoperative xerophthalmia / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM:To retrospectively analyze the effect of Qiming Granules combined with Deproteinized Calfblood Extract Eye Gel in the treatment of diabetes phaeoemulsification postoperative xerophthalmia.<p>METHODS:From June 2017 to June 2018, 147 diabetics 269 eyes were diagnosed with phaeoemulsification postoperative xerophthalmia were enrolled in our study. The control group was given the Tobramycin Dexamethasone Eye Drops and Diclofenac Sodium Eye Drops, the Sugaojie group was given Deproteinized Calfblood Extract Eye Gel on the basic of the control group, the combined group was given the Qiming Granules on the basic of the Sugaojie group. The courses all last 4wk. The corneal fluorescence staining, BUT and SⅠt were analyzed before, and post 1mo after treatment, and the clinical efficacy of each group was analyzed.<p>RESULTS: There was no difference among three groups on BUT and SⅠt before treatment(<i>P</i>>0.05). After 1 mo treatment, BUT and SⅠt of the Sugaojie group and the combined group were significantly improved compared to the control group(<i>P</i><0.05), furthermore, the combined group did better role on BUT, SⅠt and total effective rate compared to the Sugaojie group(97.9%<i> vs </i>89.0%, <i>P</i>=0.013).<p>CONCLUSION: Qiming Granules combined with Deproteinized Calf blood Extract Eye Gel could improve xerophthalmia after phaeoemulsification surgery. It is a positive and effective treatment, which has good clinical application significance.

Clinical observation of drug combined with physiotherapy in the treatment of dry eye caused by MGD / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To compare the clinical curative effects of meibomian gland dysfunction(MGD)caused by evaporative dry eye by utilizing levofloxacin eye gel and Tobramycin and Dexamethasone Ophthalmic Ointment, respectively.<p>METHODS: The 180 cases(360 eyes)with dry eye(evaporative type)caused by confirmed meibomian gland dysfunction were randomly divided into two groups(<i>i.e.</i> A and B): In group A, 90 individuals with 180 eyes were treated with levofloxacin eye gel+ sodium hyaluronate eye solution; The other 90 cases in group B took Tobramycin and Dexamethasone Ophthalmic Ointment+ sodium hyaluronate eye drops for curing the MGD. In addition, the same comprehensive therapy were used to the MGD patients in groups A and B, after surface anesthesia on binoculus, secretion, obstructing meibomian gland secretions, were discharged by utilizing cotton stick to extrusion mass weekly and four times consecutive treatments were regard as a course of treatment. To remove residual the thin oil soften lipid in meibomian gland, heat can be applied to the eyelids with hot water(around 45℃)on towel for 15min and do that three times a day. After each hot compress, we use levofloxacin eye gel to the patients in group A by dropping into the conjunctival sac and apply to the root of the eyelid lashes. The group B of 90 patients were applied Tobramycin and Dexamethasone Ophthalmic Ointment to the root of the eyelid lashes. All patients were dripped odium eye drops eye into their eyes four times a day. <p>RESULTS: After treatment(<i>Z</i>= -0.64, <i>P</i>=0.524), there were no significant differences in clinical symptoms(<i>Z</i>= -1.37, <i>P</i>=0.171), secretion characteristics(<i>Z</i>= -1.06, <i>P</i>=0.288), tear film rupture time and tear secretion time between groups A and B(<i>P</i>>0.05). Corneal fluorescence staining score: group A(cured 83.3%, improved 11.1%, ineffective 5.6%)and group B(cured 55.6%, improved 27.8%, ineffective 16.7%). The therapeutic effect of group A was better than that of group B, with statistical significance(<i>Z</i>= -4.02, <i>P</i><0.001).<p>CONCLUSION: Physical therapy for meibomian gland dysfunction caused by evaporative dry eye is given priority, and medication is treated as adjunctive therapy. Generally, the patients can achieve totally anti-inflammatory, antibacterial, safe and stable, without side effects by using levofloxacin eye gel. However, the patients with worst condition and lingering illness should cured by Tobramycin and Dexamethasone Ophthalmic Ointment. In addition, statistical significant difference is not found between the two drugs on curative effects.

Meta analysis of therapeutic effects of domestic deproteinized calf blood extract eye gel on corneal epithelial repair after laser refractive surgery / 国际眼科杂志(Guoji Yanke Zazhi)

AIM:To systemically evaluate the clinical efficacy and safety of domestic deproteinized calf blood extract eye gel for corneal epithelial repair after laser refractive surgery.METHODS:We performed a comprehensive search via Pubmed,Embase,Cochrane Library,VIP Chinese Science and Technology Journal Database,CNKI and Wan Fang Chinese periodical Database for the randomized controlled trials(RCTs) at home and abroad about effects of the domestic deproteinized calf blood extract eye gel for corneal epithelial repair after laser corneal refractive surgery with retrieval time from January 2007 to December 2016.According to the inclusion and exclusion criteria,2 medical researchers independently screened documents,extracted data and evaluated the quality.Review Manager 5.3 software was used for Meta analysis.RESULTS:Seven RCTs involving 1042 eyes,including 523 eyes in the treatment group and 519 eyes in the control group,were selected for this Meta-analysis.The results showed that the clinical efficacy in the treatment group was better than that in the control group (OR=1.81,95％CI:1.39 ～2.35;P＜0.001).And the corneal injury symptom score in the treatment group was superior to that in the control group (WMD=-0.33,95％CI:-0.45 to-0.21;P＜0.001).And the corneal healing time in the treatment group was shorter than that in the control group (WMD=-1.26,95％CI:-1.56 to-0.97;P＜0.001).CONCLUSION:The domestic deproteinized calf blood extract eye gel can relieve the patients' symptoms after laser refractive surgery,improve the corneal epithelial recovery and the efficiency of treatment.Due to the limited quality and quantity of the studies these conclusions should be further validated by more welldesigned randomized double blind controlled trials.

Vitamin A palmitate eye gel and carboxymethylcellulose sodium eye drops on prevention of dry eye after cataract surgery / 国际眼科杂志(Guoji Yanke Zazhi)

·AIM: To observe the efficacy of vitamin A palmitate eye gel and carboxymethylcellulose sodium eye drops on prevention of dry eye after phacoemulsification. ·METHODS: Ninety patients ( ninety eyes ) with age-related cataract enrolled in our hospital from March 2016 to August 2017 were performed phacoemulsification with intraocular lens implantation. They were randomly divided into three groups as control group ( n = 30 ), treated group Ⅰ ( n=30) and treated group Ⅱ ( n=30). The control group was administered with tobramycin and dexamethasone eye drops for 15d as the basis therapy. In treated group Ⅰ, patients were administered with carboxymethylcellulose sodium eye drops for 30d based on the treatment of the control group. The treated groupⅡ was administered with vitamin A palmitate eye gel for 30d on the basis of the treated group Ⅰ. The dry eye symptom score, corneal fluorescence ( FL ) staining scores, breakup time of tear film ( BUT) and Schirmer Ⅰtest ( SⅠt) without topical anesthesia were examined in 1d before operation and 15d and 30d after operation. · RESULTS: There were no statistically significant differences in subjective symptom scores of dry eye, BUT values, FL scores, and SⅠt values among the three groups before treatment (P>0. 05). After treatment, dry eye symptom scores, FL scores, and S Ⅰ t values increased at first and then decreased with time. BUT values decreased at first and then increased. Fifteen and thirty days after surgery, dry eye symptom scores, FL scores, and SⅠt values were significantly lower in the treated group Ⅰ and the treated group Ⅱ than in the control group. While BUT values were significantly higher than that in the control group. The dry eye symptom scores and S Ⅰ t value of treated group Ⅱ were significantly lower than the treated groupⅠ, and the BUT value was significantly higher than that of the treated group Ⅰ(P<0. 05). · CONCLUSION: Phacoemulsification combined with intraocular lens implantation has a certain damage of ocular surface tissue on the initial stage. The application of vitamin A palmitate eye gel combined carboxymethylcellulose sodium eye drops can improve the dry eye symptoms.

Ganciclovir eye gel and spleen ammonia peptide for epithelial herpes simplex keratitis / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To study the clinical effects of ganciclovir eye gel combined with spleen ammonia peptide oral freeze-dried powder in the treatment of epithelial herpes simplex keratitis(HSK).<p>METHODS: Totally 70 cases(70 eyes)with HSK diagnosed in Ophthalmology Department of our hospital from January 2015 to June 2016 were enrolled. All the patients were divided into control group and experimental group randomly. Control group was treated by ganciclovir eye gel, 6 times per day; while experimental group was treated by ganciclovir eye gel combined with spleen ammonia peptide oral freeze-dried powder, administered by cool white boiling water at daily bedtime, 2 milligram of every time. All patients were treated for 30d. Then the therapeutic effects of two groups, including the cure rate, time of corneal ulcer healing, pain relief, photophobia disappeared, vision improvement and recurrence rate were compared and analyzed.<p>RESULTS: The therapeutic effects of experimental group was better than the control group. The cure rate was 80% for experimental group and 51% for control group. The clinical cure rate in experimental group was significantly higher than that of the control group(<i>P</i><0.05). The time for corneal ulcer healing and pain relief and photophobia disappeared in experimental group was significantly shorter than that for the control group(<i>P</i><0.05). The recurrence rate in the 1-year follow-up was 14% for experimental group and 67% for control group. The clinical recurrence rate in experimental group was significantly lower than that of the control group(<i>P</i><0.05).<p>CONCLUSION: The application of ganciclovir eye gel combined with spleen ammonia peptide oral freeze-dried powder in epithelial herpes simplex keratitis, which improves visual acuity, the cure rate and shortens the course of disease and reduces the recurrence rate, is the ideal treatment of epithelial herpes simplex keratitis at present.

Efficacy of carbomer eye gel combined with hydroxycitron eye drops in the treatment of dry eye / 中国生化药物杂志

Objective To observe the efficacy of carbomer eye gel combined with hydroxycitron eye drops in the treatment of dry eye.Methods214 cases with xerophalmi in Yongkang first people's hospital were divided into the CT group and the CL group according to the admission of odd and even numbers.The CL group were given carbomer eye gel, and the CT group were given carbomer eye gel combined with hydroxycitron eye drops.The effect, 3 index of tear film stability and quality of life were evaluated in the two groups.ResultsThe effect in the CT group was significantly higher than that in the CI group (P<0.05).Tear break-up time(BUT) and schirmer test(SIT) in the CT group were significantly better than those in the CL group(P<0.05), Fluorescent Stain Test (FLT) in the CT group was lower than that in the CL group.After treatment, at the seventh day, the fourteenth day, the twenty-eighth day, the quality of life in the CT group was significantly higher than that in the CL group.ConclusionIt can significantly relieve the clinical symptoms which carbomer eye gel combined with Hypoglycemic eye drops Dextran 70 and Glycerol Eye Drop on the treatment of xerophthalmia, and it is worthy of promotion.

Efficacy of atropine and anisodamine eye drops for adolescent pseudomyopia / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM:To investigate the effect and local influence of atropine and anisodamine eye drops on adolescent pseudomyopia. <p>METHODS:Totally 110 cases of juvenile pseudomyopia were randomly divided into two groups, the control group was given 10g/L atropine sulfate eye gel, and the observation group was treated with 5g/L raceanisodamine eye drops. The efficacy of two methods, the changes of axial length and intraocular pressure before and after treatment, and the incidence of adverse reactions were compared. <p>RESULTS: There was no significant difference in cure rate between the two groups(<i>χ</i>²=0.533, <i>P</i>=0.465), but the effective rate of observation group was significantly better than the control group(<i>χ</i>²=3.907, <i>P</i>=0.048). Compared with the same group before treatment, the length of the axial length of the two groups increased in different degrees,and the increase value of the observation group was significantly higher than that of the control group, the difference was statistically significant(<i>P</i><0.05), however, there was no significant difference between the two groups after treatment(<i>P</i>>0.05). The intraocular pressure of the two groups was significantly lower than that of the same group before treatment, and the difference between the two groups after treatments was not statistically significant(<i>P</i> >0.05). The incidence of adverse reactions in the observation group was significantly lower than that in the control group(<i>χ</i>²=18.939, <i>P</i><0.05). <p>CONCLUSION: Anisodamine eye drops in the treatment of juvenile pseudomyopia has obvious curative effect, its efficacy and safety are better than atropine eye gel.

Effect of carbomer eye gel combined with polyethylene glycol eye drops on the dry eye / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate the clinical effect of carbomer eye gel combined with polyethylene glycol eye drops on the treatment of dry eye. ·METHODS:Totally 120 dry eye patients(240 eyes) were randomly divided into the observation group ( n = 60 cases) and the control group ( n=60 cases) . Two groups were given the polyethylene glycol eye drops. On the basis of this, the observation group were given the carbomer eye gel. The course of treatment was 1mo. The levels of IL-1β and TNF-α, eye symptom score [ ocular surface disease index ( OSDI ) , break-up time ( BUT ) , Schirmer Ⅰ test ( SⅠt ) , corneal fluorescein staining ( FL) ] , efficacy and adverse reactions in two groups were compared. ·RESULTS: After treatment, the levels of IL-1β and TNF-α, OSDI and FL in two groups were significantly lower than those before treatment and BUT and SⅠT were significantly higher than those before treatment, showing statistically significant differences ( P< 0. 05 ). Moreover, the levels of IL-1β and TNF-α, OSDI, BUT, SⅠT, FL in the observation group were improved better than those in the control group ( P<0. 05 ). The total effective rate of the control group was significantly lower than that of the observation group, indicating statistically significant difference (x2=5. 065, P=0. 024). There were no ocular symptoms and drug intolerance in two groups. · CONCLUSION: Carbomer eye gel combined with polyethylene glycol eye drops has obvious curative effect on dry eye, which is better than that treated by polyethylene glycol eye drops, and this combination does not increase the adverse reactions.

Preparation, in vitro and in vivo studies of Fraxini cortex thermosensitive in-situ-forming eye gel / 中国药学杂志

OBJECTIVE: To study on characterization, irritation, the release mechanism and the elimination kinetics of Fraxini Cortex thermosensitive in-situ-forming eye gel (FC-ISG). METHODS: The non-membrane dissolution model was used to observe the release mechanism of FC-ISG. The stabilities of FC-ISG were investigated under following circumstances bright light, freeze test and accelerating test. Single-dose and multiple-dose irritations of FC-ISG were evaluated by draize test. The elimination kinetics of FC-ISG were analyzed by non-compartment model. RESULTS: FC-ISG showed good stability and non-stimulation to rabbit eyes. Drug release from FC-ISG was completely controlled by gel erosion, the release kinetics was coincided with zero-level release. AUC and MRT in FC-ISG group were significantly higher than those in control group (P<0.05). CONCLUSIONS: FC-ISG can improve the bioavailability of drug by prolonging the residence retention time of drug in cornea. FC-ISG shows a great potential in ocular application.

Efficacy of levofloxacin hydrochloride eye gel in treatment of staphylococcus aureus keratitis in rabbits / 第二军医大学学报

Objective To observe the efficacy of levofloxacin hydrochloride eye gel (LHEG) in treatment of Staphylococcus aureus keratitis in rabbits. Methods Sixty rabb received intrastromal injection of 10 μL bacterial suspension conta establish Staphylococcus aureus keratitis model. Forty-eight rabb into four groups; 24 h baseline group, phosphate buffer solution (PBS) control group, levofloxacin hydrochloride eye drop (LHED) group, and LHEG group. Rabbits in the 24 h baseline group received slit-lamp examination and were given clinical score, and their corneas were collected for histopathology examination and bacterial quantitation. The other three groups received treatment with PBS, LHED or LHEG three times a day for seven successive days. In the last day, rabbits in these three groups received the same examination as that in the 24 h baseline group. The clinical scores and cornea bacterial quantitation were analyzed with variance analysis, and a P value less than 0. 05 was considered as statistically significant Results Compared wth PBS control group, clinical symptoms of LHED group and LHEG group were significantly reduced (P = 0. 00) on the seventh day, with the clinical scores being (4. 21 ± 1. 10) and (3. 63±0. 86), respectively; the numbers of bacterial colonies were also significantly reduced in LHED group and LHEG group (P =0. 00), with the numbers being (4. 87 ± 0. 05) and (4. 64±0. 10) CFU, respectively. Moreover, LHEG group had a significantly better bacteriostatic effect than LHEG group (P =0. 00). Conclusion Local application of LHEG can greatly inhibk the growth of bacterial colony of Staphylococcus aureus and improve the clinical signs of keratitis, with the efficacy being better than that of routinely used LHED in rabbk model of Staphylococcus aureus keratitis.

Human plasma exposure level of gatifloxacin eye gel / 中国药学杂志

OBJECTIVE: To determine gatifloxacin in human plasma, and study the exposure characteristics of gatifloxacin in Chinese healthy male volunteers who used Gatifloxacin Eye Gel for 7 d. METHODS: LC-MS/MS method was developed for the quantitation of gatifloxacin in human plasma After protein precipitation with methanol and dilution with water, the chromatographic separation was carried out on an Symmetry C18 column (4.6 mm × 100 mm, 5 μm) with a gradient mobile phase consisting of 0.1% formic acid in methanol and 0.1% formic acid in water at a flow rate of 0.5 mL · min-1. The quantitation analysis was performed using multiple reaction monitoring (MRM) at the specific ion transitions of m/z 376.2→261.0 for gatifloxacin and m/z 332.1→-288.1 for ciprofloxacin (internal standard) in the positive ion mode with electrospray ionization (ESI) source. Specificity, linearity, precision and accuracy, matrix effects, recovery and stability were investigated to validate the LC-MS/MS method. Plasma samples of different times from 10 Chinese healthy male volunteers were determined, who used Gatifloxacin Eye Gel for 7 d. RESULTS: The linear range was 2 -500 ng · mL-1. The intra- and inter-day precisions were less than 2.61% and 13.1%, and the accuracy was within ±5.0%. Matrix effective, recovery, specificity and stability were within ± 15.0%. The concentration of gatifloxacin in human plasma was below the lower limit of quantitation (2 ng · mL-1). CONCLUSION: This method is sensitive and accuracy for the determination of gatifloxa-cinin human plasma, and the plasma gatifloxacin level are all below the lower limit of quantitation (2 ng · mL-1) in all subjects.

Clinical research of protein-free calf blood extract eye gel for corneal epithelial defect / 国际眼科杂志(Guoji Yanke Zazhi)

AlM: To evaluate the clinical effect of 200g/L protein-free calf blood extract eye gel for corneal epithelial defect.METHODS: One hundred and sixty - eight cases of corneal epithelial defect ( 58 cases with herpes simplex keratitis; 24 cases with chemical injury; 85 cases with pterygium operation injury ) were randomly divided into two groups: 84 eyes were treated with protein-free calf blood extract eye gel; 84 cases were treated with basic fibroblast growth factor eye gel ( bFGF ) . The bFGF and protein-free calf blood extract eye gels were used 4 times a day. The treatment course was 7d. Epithelial defect restoration, local symptom and sign were observed. RESULTS: The difference between pre-treatment and post-treatment was significant ( P CONCLUSlON: Protein-free calf blood extract eye gels is valuable and safe for corneal epithelial defect.

Clinical study Carbomer eye gel and rhEGF in treating dry eye after cataract surgery in diabetics / 国际眼科杂志(Guoji Yanke Zazhi)

To investigate the effect of Carbomer eye gel and recombinant human epidermal growth factor (rhEGF) on dry eye after cataract surgery in diabetics. ●METHODS: A total of 160 patients ( 160 eyes ) with diabetes undergone phacoemulsification with a diagnosis of dry eyes were randomized divided into two groups, treatment group (80 cases, 80 eyes) and control group (80 cases, 80 eyes). ln treatment group, the patients were treated by Carbomer eye gel and rhEGF. ln control group, the patients were treated by Carbomer eye gel. The subjective dry eye symptoms, tear break - up time (BUT), Schirmer l test ( S Ⅰ t) and cornea fluorescein staining ( FL) were observed before treatment, 1, 4wk after treatment. ●RESULTS: There was no significant difference between groups before treatment (P> 0. 05). After treatment at 1, 4wk, the subjective dry eye symptoms and the results of three examination ( BUT, S Ⅰ t and FL) in two groups better than those before treatment ( P ●CONCLUSlON: Carbomer eye gel can relieve the dry eye symptoms of diabetes after phacoemulsification effectively, and it can be more effective to combine Carbomer eye gel with rhEGF at early stage after phacoemulsification.

Therapeutic effect of deproteinised calf serum eye gel on corneal alkali burn / 中华实验眼科杂志

Background Corneal chemical burns,especially hazards of alkali burn become increasingly prominent.Clinically,anti-inflammatory,immuno-suppression,corneal transplantation are the common treating method for corneal alkali burn.But the research of tissue repair under the microenvironment of corneal alkali burn is necessary.Objective The aim of this study was to investigate the therapeutic effect of deproteinised calf serum eye gel on the corneal alkali burn.Methods Alkali burn model of cornea was established on the right eyes by putting the filter paper with 0.5 mol/L NaOH on the center cornea for 1 minute in 24 white rabbits.The model rabbits were divided randomly into 4 groups.Normal saline solution,deproteinised calf serum eye gel,blank matrix gel or recombinant bovine basic fibroblast growth factor(rb-bFGF)eye-gel was topically administered 4 times per day for 14days in the 4 groups,respectively.The inflammatory reaction was examined under the slit lamp and scored based on Ando' s criteria.Corneal fluorescine staining was performed to calculate the corneal ulcer area and scored based on Trousdale' s criteria.Histopathological examination of corneas was performed on the fourtcenth day after experiment.The use of the experiment animals complied with ARVO Statement.Results Corneal edema and opacification were seen in the model eyes with the modeling successful rate 100％.On the seventh day after experiment,the severe ulcer of cornea and hypopyon appeared in the normal saline solution group.Corneal epithelium was intact but the intrarocular structure was invisible in the blank matrix gel group.In th(c) rb-bFCF group,corneal new vessels were seen,however,the corneal ulcer completely regrow in the deproteinised calf serum eye gel group.In 3,5,7,10 and 14days after examination,the corneal inflammatory scores were significantly lower in the deproteinised calf serum eye gel group and rb-bFGF group than those of the normal saline solution(P＜0.01).No significant difference was found in the inflammatory score between the deproteinised calf serum eye gel group and rb-bFGF group (P＞0.05) but was significantly lower than the blank gel matrix group (P ＜ 0.05).With respect to the corneal ulcer,the score was decreased in the deproteinised calf serum eye gel group compared with the normal saline solution group and blank gel matrix group (P ＜ 0.05).Howcver,no significant difference was found in the corneal ulcer score between the deproteinised calf serum eye gel group and rb-bFGF group in various time points (P＞ 0.05).Conclusions Deproteinised calf serum eye gel can promote the healing of corneal ulcer and remit the inflammatory response afler corneal alkali burns with a better effectiveness than rb-bFGF.

Drug Release Characteristics of Mu'an-Eye-Gel in Vitro / 中国药房

OBJECTIVE:To compare home-made mu'an-eye-gel(acyclovir plus honey) with commercial aciclovir(ACV)-eye-gel in releasing drug characteristics in vitro.METHODS:The in vitro drug release test was conducted by the third method of dissolution determination stated in Chinese Pharmacopeia together with bag filler method.The cumulative drug-releasing percentage and the acyclovir amount in mu'an-eye-gel versus ACV-eye-gel were determined by UV spec-trophotometry,and the accumulative releasing drug percentages of the two preparations were computed and their drug release behaviors w ere compared.RESULTS:The in vitro releasing behaviors of mu'an-eye-gel followed the Weibull kinetic equa-tion,however the vitro releasing behavior of commercial ACV-eye-gel followed the zero order kinetic equation,and the T80%and Q8 h had statistical significances between(mu'an-eye-gel:T80%=3.156?0.013(h),Q8 h=93.28?0.010(%);ACV-eye-gel:T80%=10.16?0.009(h),Q8 h=67.85?0.025(%)) 2 kinds of preparation.CONCLUSION:Mu'an-eye-gel is superior to the commercial ACV ophthalmic gel in both releasing velocity and accumulative drug release percentage.