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Clinics ; Clinics;66(9): 1621-1625, 2011. ilus, tab
Статья в английский | LILACS | ID: lil-604304

Реферат

OBJECTIVE: To evaluate the effect of pneumoperitoneum, both alone and in combination with controlled ventilation, on peritoneal lymphatic bacterial clearance using a rat bacterial peritonitis model. METHOD: A total of 69 male Wistar rats were intraperitoneally inoculated with an Escherichia coli solution (109 colony-forming units (cfu)/mL) and divided into three groups of 23 animals each: A (control group), B (pneumoperitoneum under 5 mmHg of constant pressure), and C (endotracheal intubation, controlled ventilation, and pneumoperitoneum as in Group B). The animals were sacrificed after 30 min under these conditions, and blood, mediastinal ganglia, lungs, peritoneum, liver, and spleen cultures were performed. RESULTS: Statistical analyses comparing the number of cfu/sample in each of the cultures showed that no differences existed between the three groups. CONCLUSION: Based on our results, we concluded that pneumoperitoneum, either alone or in association with mechanical ventilation, did not modify the bacterial clearance through the diaphragmatic lymphatic system of the peritoneal cavity.


Тема - темы
Animals , Male , Rats , Diaphragm/microbiology , Escherichia coli Infections/microbiology , Lymphatic System/microbiology , Pneumoperitoneum, Artificial , Peritoneal Cavity/microbiology , Peritonitis/microbiology , Respiration, Artificial , Blood/microbiology , Colony Count, Microbial , Disease Models, Animal , Escherichia coli Infections/surgery , Peritonitis/surgery , Pneumoperitoneum, Artificial/methods , Rats, Wistar
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