Cranial bone regeneration with collagen type I and polyvinylpyrrolidone (Fibroquel®) combined with hyaluronic acid in Wistar rats: morphological study / Regeneración ósea craneal con colágeno tipo I y polivinilpirrolidona (Fibroquel®) combinado con ácido hialurónico en ratas Wistar: estudio morfológico

SUMMARY: The purpose of this study was to evaluate by morphological methods, if a mixture of Fibroquel® and hyaluronic acid implanted in an animal model of cranial bone injury could promote bone regeneration. 12 Wistar rats were divided in three groups, control group, bone injury without treatment and bone injury with treatment. After experimental period, bone samples were taken and stained with H & E, Masson trichrome, PAS-D, immunohistochemistry with anti-PCNA monoclonal antibody and applied a semiquantitative morphometric method. Treatment group showed extensive areas of collagen fibers in contact with normal bone tissue, areas of normal histology, PAS positive material and less cellular proliferation. We demonstrated for the first time that a mixture of Fibroquel® and hyaluronic acid implanted in an animal model of cranial bone injury promotes bone regeneration.

RESUMEN: El propósito de este estudio fue evaluar por métodos morfológicos, si una mezcla de Fibroquel® y ácido hialurónico implantado en un modelo animal de lesión del hueso craneal podría promover la regeneración ósea. Se dividieron 12 ratas Wistar en tres grupos, grupo control, lesión ósea sin tratamiento y lesión ósea con tratamiento. Después del período experimental, se tomaron muestras de hueso y se tiñeron con H & E, tricrómico de Masson, PAS-D, inmunohistoquímica con anticuerpo monoclonal anti-PCNA y se aplicó un método morfométrico semicuantitativo. El grupo de tratamiento mostró áreas extensas de fibras de colágeno en contacto con tejido óseo normal, áreas de histología normal, material PAS positivo y menor proliferación celular. Demostramos por primera vez que una mezcla de Fibroquel® y ácido hialurónico implantado en un modelo animal de lesión del hueso craneal promueve la regeneración ósea.

In vitro plantlet regeneration from seedling nodal explants of Acacia catechu.

Multiple shoots were initiated after 20 days in stem nodes excised from in vitro grown seedlings of Acacia catechu, on Murashige and Skoog's medium adjuvanted with 1 to 100 microM of N6-benzyladenine (BA). Explants were subcultured on the same medium augmented with 1.5 g l(-1) of polyvinylpyrrolidone (PVP) after 30 days. In the second subculture, after 30 days, the explants were transferred to a medium lacking PVP, but containing 10 microM of BA, where nine or ten shoots differentiated per explant within next 30 days. If individual shoots along with some callus were subcultured on BA (10 microM), nearly 15 shoots per explant regenerated in 90 days. Thus, the average number of shoots obtained from each node was 142 after 180 days. Since a seedling develops four nodes after 20 days, theoretically an average of 568 shoots can be obtained from a single seed. If shoots were individually subcultured on 1/2-strength MS medium with 14.7 microM of indole-3-butyric acid (IBA), roots developed in 20 days. Addition of 40 mg l(-1) of glutamic acid to the rooting medium prevented leaf senescence. These plantlets thrived well in garden soil, sand and silica (1:1:1).

Effect of anti-oxidants and adsorbents on tissue browning associated metabolism in Cocculus pendulus callus cultures.

Explants and callus of C. pendulus produced intense brown substances in the medium which caused necrosis. Various anti-oxidants (ascorbic acid, cysteine and dithiothreitol) and adsorbents (activated charcoal and polyvinyl pyrrolidone) were used in different concentrations to prevent browning of the tissues. These in MS medium affected differently the growth, colour and texture of the tissues. It was concluded that both peroxidase and phenolase were involved in the browning. Increased peroxidase activity and decreased phenolase activity were probably due to more peroxidative oxidation of phenols and unavailability of substrate for phenolase activity. This resulted in faster growth of tissues, which further reduced the phenolase activity.

Cuidados e anti-sepsia da cavidade bucal / Methods of asepsis and antisepsis related to oral procedures

Os autores apresentam os principais cuidados relacionados a procedimentos de anti-sepsia na cavidade bucal, abordando esterilizaçäo dos instrumentos e materiais usados, desinfecçäo, anti-sepsia e proteçäo individual. Os principais anti-sépticos utilizados na cavidade bucal säo a cloro-hexidina e os compostos da polivinilpirrolidona associados ao iodo

Efectos del tratamiento con polivinilpirrolidona (PVP) sobre la calidad nutricional del sorgo / Effects of the treatment with polyvinylpyrrolidone on nutritional quality of the sorghum

En este trabajo los autores se propusieron comprobar que el elevado contenido de compuestos polifenólicos en el grano de sorgo (Sorghum saccharatum, var. sugar drip) ejerce una influencia desfavorable sobre la absorción nitrogenada. Para arribar a esta conclusión, se disminuyó el contenido de taninos de la harina de sorgo mediante tratamiento con polivinilpirrolidona (PVP), como complejante. Se realizaron experiencias biológicas con harina sin tratamiento (S), y tratada con PVP (S + PVP). El tratamiento mejoró la digestibilidad verdadera (D), obteniéndose los valores P < 0.01). La valoración de taninos arrojó estos resultados: S = 1.90g/100g, y S + PVP = 0.85g/100g, informados como ácido tánico. El aumento de digestibilidad se tradujo en un mejor aprovechamiento nitrogenado, siendo los valores de utilización proteínica neta (NPU) para S y S + PVP de 19 ñ 1.58 y 37 + 3.36, respectivamente (significación P < 0.001)