摘要
The purpose of this work was to evaluate the antioxidant capacity of Prosopis pallida (Fabaceae) fruits "algarrobo", in addition to determine their main chemical components. Fruit purified extracts from the regions of Tumbes, Piura, Lambayeque, and La Libertad (Peru) were evaluated using infusion, decoction, and alcoholic extraction at concentrations of 45, 70 and 96%. To measure the antioxidant capacity of the extracts, the determination of total phenolics content was performed, followed by the antioxidant evaluation using DPPH, FRAP and ABTS. The chemical identification was carried out through UHPLC-UV-MS/MS. The results showed that the decoction of the sample from the region of La Libertad, as well as the ethanol extracts at 45% of the samples from Tumbes and Lambayeque presented greater antioxidant capacity. Moreover, ten major substances of these extracts were identified: nine phenolic derivatives (vicenin II as the main constituent of the extracts) and one diterpene (7-oxodehydroabietic acid).
El propósito de este trabajo fue evaluar la capacidad antioxidante de frutos de Prosopis pallida (Fabaceae) "algarrobo", además de determinar sus principales componentes químicos. Se estudiaron extractos purificados de frutos de las regiones de Tumbes, Piura, Lambayeque y La Libertad (Perú), mediante infusión, decocción y extracción alcohólica en concentraciones de 45, 70 y 96%. Para medir la capacidad antioxidante de los extractos, se realizó la determinación del contenido de fenoles totales, seguida de la evaluación antioxidante mediante DPPH, FRAP y ABTS. La identificación química se realizó mediante UHPLC-UV-MS/MS. Los resultados mostraron que la decocción de la muestra de la región de La Libertad, así como los extractos de etanol al 45% de las muestras de Tumbes y Lambayeque presentaron mayor capacidad antioxidante. Además, se identificaron diez sustancias principales de estos extractos: nueve derivados fenólicos (vicenin II como componente principal de los extractos) y un diterpeno (ácido 7-oxodehidroabiético).
Subject(s)
Plant Extracts/pharmacology , Plant Extracts/chemistry , Prosopis/chemistry , Antioxidants/pharmacology , Peru , Plants, Medicinal , Medicine, Traditional摘要
The chemical composition of Mangifera indica L. cv. "Kent" leaves was determined by HPLC-ESI-QTOF-MS/MS. Polyphenolic compounds characterized as benzophenone derivatives were the main components found in extracts (1, maclurin 3-C-(2-O-galloyl)-D- glucoside isomer; 2, maclurin 3-C-ï¢--D-glucoside; 3, iriflophenone 3-C-ï¢--D-glucoside; 5, maclurin 3-C-(2,3-di-O-galloyl)-ï¢--D-glucoside; 6, iriflophenone 3-C-(2-O-galloyl)-ï¢--D-glucoside; 7, methyl-iriflophenone 3-C-(2,6-di-O-galloyl)-ï¢--D-glucoside) and xanthones (4, mangiferin and 8, 6-O-galloyl-mangiferin). The estrogenic and antioxidant effects of aqueous extracts from Mangifera indica L. cv. "Kent" leaves on ovariectomized rats were determined by uterotrophic assay and malondialdehyde (MDA) levels in erythrocytes, bone, liver, and stomach. We conclude that the polyphenolic compounds from extracts act as exogenous antioxidant agents against oxidative damage in ovariectomized rats.
La composicioÌn quiÌmica de las hojas de Mangifera indica L. cv. "Kent" se determinoÌ por HPLC-ESI-QTOF-MS/MS. Compuestos polifenoÌlicos caracterizados como derivados de benzofenona fueron los componentes principales encontrados en los extractos (1, isoÌmero de la maclurina 3-C-(2-O-galoyil)-D-glucoÌsido; 2, maclurina 3-C-ß-D-glucoÌsido; 3, iriflofenona 3-C-ß-D-glucoÌsido; 5, maclurina 3-C-(2,3-di-O-galloiÌl)-ß-D-glucoÌsido; 6, iriflofenona 3-C-(2-O-galloil)-ß-D-glucoÌsido; 7, metil-iriflofenona 3-C-(2,6-di-O- galloyl)-ß-D-glucoÌsido) y xantonas (4, mangiferina y 8, 6-O-galoyil-mangiferina). Los efectos estrogeÌnicos y antioxidantes de los extractos acuosos de hojas de Mangifera indica L. cv. "Kent" en ratas ovariectomizadas se determinaron mediante ensayo uterotroÌfico y la medicioÌn de los niveles de malondialdehiÌdo (MDA) en eritrocitos, huesos, hiÌgado y estoÌmago. Concluimos que los compuestos polifenoÌlicos de los extractos actuÌan como agentes antioxidantes exoÌgenos contra el danÌo oxidativo en ratas ovariectomizadas.
Subject(s)
Animals , Female , Rats , Plant Extracts/pharmacology , Plant Extracts/chemistry , Ovariectomy , Mangifera/chemistry , Estrogens/pharmacology , Antioxidants/pharmacology , Stomach/drug effects , Benzophenones/chemistry , Bone and Bones/drug effects , Lipid Peroxidation/drug effects , Chromatography, High Pressure Liquid , Reactive Oxygen Species , Rats, Sprague-Dawley , Plant Leaves/chemistry , Spectrometry, Mass, Electrospray Ionization , Ethanol , Tandem Mass Spectrometry , Liver/drug effects , Malondialdehyde , Antioxidants/chemistry摘要
ABSTRACT Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell). Antioxidant activity was determined using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbing capacity (ORAC) assays and total phenolic, flavonoid and tannin contents were determined by spectrophotometric techniques. Ethyl acetate extract of M. floribunda exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 69.70 and 172.10 µg/mL. For HaCat cell, TGI value was 213.60 µg/mL. M. floribunda showed a strong antioxidant potential: EC50 of 45.89±0.42 µg/mL and 0.55±0.05 mmol TE/g for DPPH and ORAC, respectively. Total phenolic content was 0.23±0.013g gallic acid equivalents (GAE)/g extract and exhibited 13.10±1.60% of tannins content. The content of flavonoid was 24.08±0.44% expressed as rutin equivalents. These results provide a direction for further researches about the antitumoral potential of M. floribunda.