摘要
A relação entre Helicobacter spp. e a presença de alterações histológicas na pars esophagea de suínos foi avaliada em 67 estômagos de animais em idade de abate. Para a identificação das helicobactérias, utilizou-se a técnica da PCR com primers específicos para o gênero Helicobacter. As alterações histológicas foram identificadas e classificadas como ulceração, erosão, degeneração epitelial, alongamento de papilas, hiperplasia, paraqueratose, intensidade do infiltrado inflamatório e aumento do número de folículos linfoides. As alterações mais frequentemente encontradas na pars esophagea foram a degeneração epitelial e o alongamento de papilas, observadas em 83,5 por cento (n=56) das amostras analisadas. Em 77,5 por cento (n=52) das amostras, observou-se paraqueratose e em 61,1 por cento (n=41) hiperplasia epitelial. Quarenta e sete (70,1 por cento) foram positivas na PCR para Helicobacter spp. Nessas amostras a erosão foi a lesão mais observada (40,2 por cento), seguida de ulceração da mucosa (11,9 por cento). Em 58,2 por cento das amostras positivas na PCR, não foram observadas ulcerações de mucosa. Observou-se associação significativa (P=0,003) entre a presença de Helicobacter spp. e a degeneração epitelial da pars esophagea de suínos em idade de abate.
The association between histological findings of gastric mucosa in pigs at slaughtering age and the presence of Helicobacter spp., identified by PCR, assay was investigated. Stomachs from 67 pigs were examined. Histological changes of pars esophagea were identified and classified as gastric ulcers, erosion, degeneration, distortion of papils, hyperplasia, paraqueratosis, and number of lymphoid follicles. Microscopic analysis revealed the most frequent alteration: 83.5 percent (n= 56) stomachs with epithelial degeneration and distortion of papils. Paraqueratosis of pars esophagea was observed in 77.5 percent (n=52) of the samples and epithelial hyperplasia in 61 percent (n=41). Forty-seven (70.1 percent) pigs were positive to Helycobacter spp. by PCR. Erosion of pars esophagea and ulceration were the most frequent findings in Helicobacter spp. PCR-positive pigs, occurring, respectively, in 40.2 percent and 11.9 percent. The frequency of animals without ulceration and Helicobacter spp. PCR-positive was 58.2 percent. It was observed a significant association (P=0.003) between Helicobacter spp. and epithelial degeneration of gastric mucosa in pigs at slaughtering age.
Subject(s)
Animals , Stomach/anatomy & histology , Stomach/pathology , Stomach Diseases/veterinary , Helicobacter/isolation & purification , Polymerase Chain Reaction/methods , Swine , Stomach Ulcer/veterinary摘要
Helicobacter pylori is the most common gastric bacteria of human beings. Animal-borne helicobacter have been associated with gastritis, ulceration, and gastric mucosa-associated lymphoid-tissue lymphoma in people. We attempted to identify the species of Helicobacter spp. that infect human beings in north Paraná, Brazil. Samples of gastric mucosa from 38 dyspeptic patients were analyzed by optic microscopy on silver stained slides, polimerase chain reaction (PCR), and enzymatic cleavage. Genus and species-specific primers to H. pylori, H. heilmannii, H. felis, and consensual primers to H. bizzozeronii or H. salomonis were used. The PCR products were submitted to enzymatic cleavage by VspI (Helicobacter spp. product) and HinfI (species products) enzymes. Thirty-two out of 38 patients evaluated had 3.2 to 5 æm long bacteria that resembled H. pylori in Warthin-Starry stained slides and were positive to the genus Helicobacter by PCR. In 30 of these patients the bacteria were identified as H. pylori. Two samples positive by silver stain were negative to all species tested by PCR. None of the 38 samples was positive to animal-origin helicobacter species. These results show that PCR and enzymatic restriction are practical methods to identify the species of helicobacters present in gastric mucosa of human beings. People in north Paraná appear to be infected mostly with H. pylori
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Dyspepsia , Helicobacter , Helicobacter Infections , Aged, 80 and over , Brazil , DNA, Bacterial , Gastric Mucosa , Helicobacter , Polymerase Chain Reaction摘要
O uso da via intramedular foi avaliado em 31 cäes clinicamente normais, de até três meses de idade e com peso entre 920 e 3.385 g, divididos, aleatoriamente, em grupo 1 (G1), com 15 animais, e grupo 2 (G2), com 16. Aqueles do G1 foram tratados com NaCl a 0,9 por cento e os do G2 com sangue total. Foram anotados os tempos gastos para implantaçäo da agulha, as velocidades máximas de infusäo obtidas e as ocorrências havidas durante o período de infusäo. Todos os animais foram avaliados clinicamente durante os 30 dias de experimentaçäo e por meio de radiografias nos dias 15 e 30 do experimento. Constatou-se que a técnica é de fácil e rápida execuçäo e pouco cruenta. Os resultados observados indicam que a via intramedular é segura, sendo uma alternativa valiosa à via intravenosa no tratamento de filhotes de cäes com graves alteraçöes hemodinâmicas