摘要
<p><b>OBJECTIVE</b>To study tissue culture and plant regeneration of Withania somnifera.</p><p><b>METHOD</b>Leaves of W. somnifera were used for explants, effects of different plant growth substances on callus and shoot induction were studied, different medium and plant growth substances for rooting induction was optimized.</p><p><b>RESULT AND CONCLUSION</b>The best plant growth substances combination for callus induction was MS + 1.0 mg x L(-1) 2,4-D + 0.1 mg x L(-1) KT. The optimal medium for germination was MS + 1.0 mg x L(-1) 6-BA + 0.1 mg x L(-1) NAA. The best medium and plant growth substances combination for rooting induction was 1/2MS + 0.5 mg x L(-1) NAA, transplant survival rate of plantlet reached 92% in humus soil-pearlite (1:1).</p>
Subject(s)
Culture Media , Pharmacology , Plant Growth Regulators , Pharmacology , Regeneration , Tissue Culture Techniques , Withania摘要
This paper summarized and analyzed the reforming trends in ideas,methods and contents of morphology laboratory education in basic medical school.In order to fulfill the education goals of imparting knowledge and fostering capability and quality education,we should combine the traditional and virtual methods effectively,transform the secondary status ( verifying lecture) of morphology laboratory teaching to independent subject,try to integrate the subjects of morphology,use bilingual education and cultivate scientific research ability.
摘要
This article analyzes and discusses histology's teaching mode and condition,to put forward a conceiving of altering classical modal to first experimental classes and then theoretical classes.The feasibility and expecting effect of new teaching model are concluded