摘要
With the development of transcriptomic technologies such as gene chip technology and RNA sequencing technology, important related factors in the pathogenesis of atopic dermatitis (AD) have been gradually identified, such as different T helper (Th) cell subtypes and other immune-related cells (macrophages and Langerhans cells) ; abnormal changes in active substances such as interleukin-4, interleukin-13, fillagrin and loricrin released by immune-related cells such as Th2 cells and keratinocytes have been found to play major roles in pruritus and skin barrier damage in AD. In recent years, transcriptomic technologies have been applied to the analysis of changes in transcriptomic profiles of patients before and after treatment to evaluate patients′ condition and therapeutic effect. This review summarizes research progress in transcriptomics in AD in recent years.
摘要
Epidermal barrier defects and immune abnormalities are the main pathophysiological changes in the development of atopic dermatitis (AD) . Skin keratinocytes can release a variety of inflammatory factors and mediators under the treatment with various harmful factors. Three epithelium-derived cytokines interleukin (IL) -33, IL-25 and thymic stromal lymphopoietin are considered to be effective inducers of Th2 immune response in skin or mucosal barrier, which can activate immune cells, cause the secretion of Th2 cytokines, enhance the Th2 immune response, and participate in the occurrence and development of AD. This review focuses on the role of the above 3 epithelium-derived cytokines in the pathogenesis of AD.
摘要
A pedigree with familial acanthosis nigricans presenting with atypical clinical symptoms was reported. The 4-year-old female proband began to develop black patches on the neck and abdomen since the age of 1 year, which had gradually spread to the lips and front of the trunk in recent years. Reflectance confocal microscopy of the abdominal skin showed downward extension and twisting of dermal papillary rings with formation of gully-like structures, and moderately to highly refractive particles in the dermal papillary rings. The proband′s father and grandmother had similar medical history, but the pigmentation spontaneously subsided with age, leaving only local thickened skin lines. Peripheral blood samples were collected from the proband, her parents and grandmother, and panel-based targeted sequencing of peripheral blood DNA was performed for the proband. Sequencing showed a missense mutation c.1949A>C (p.Lys650Thr) in exon 14 of the FGFR3 gene in the proband, and Sanger sequencing confirmed the presence of this mutation in the proband and her father and grandmother. A diagnosis of familial acanthosis nigricans was made.
摘要
Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway plays an important role in immune pathways in atopic dermatitis (AD) . Drugs that block the JAK-STAT signaling pathway, such as classic JAK inhibitors tofacitinib, ruxolitinib, etc., have been gradually applied to the treatment of AD in clinical trials, and good clinical efficacy has been achieved. In addition, other inhibitors of the JAK-STAT signaling pathway, such as apamin and dupilumab, also show some efficacy in the treatment of AD. This review summarizes recent studies on the JAK-STAT signaling pathway and its inhibitors.
摘要
Objective To investigate factors influencing disease severity in children of Han nationality with atopic dermatitis (AD) in China,and to provide scientific evidences for prevention andtreatment of AD in children.Methods From November 2005 to May 2015,data were collected from AD children aged 0-12 years in Department of Dermatology,The First Affiliated Hospital of Anhui Medical University and AD sample collection collaboration network in China.Statistical analysis was carried out with SPSS16.0 software by using univariate and multivariate ordinal logistic regression analysis to investigate factors influencing the severity of AD.Results A total of 2 620 children with AD were enrolled into the study,including 230 (8.8%) with mild AD,1 379 (52.6%) with moderate AD and 1 011 (38.6%) with severe AD.As univariate analysis showed,factors influencing the severity of AD included region,early onset,itching during sweating,xeroderma,ichthyosis,palmar hyperlinearity,lichen pilaris,orbital darkening,scalp dermatitis and infra-auricular fissure (all P < O.O1).There were no significant differences in the severity of AD between different genders,patients with and without personal or family atopic history,or between patients with and without a high serum IgE level (all P > 0.05).Multivariate ordinal logistic regression analysis showed that southern region (OR =2.10,95% CI:1.76-2.51),early onset (OR =1.32,95% CI:1.01-1.71),itching during sweating (OR =1.70,95% CI:1.42-2.02),xeroderma (OR =2.57,95% CI:2.10-3.15),ichthyosis (OR =1.49,95% CI:1.19-1.87),palmar hyperlinearity (OR =2.03,95% CI:1.61-2.57),lichen pilaris (OR =1.44,95% CI:1.17-1.78),orbital darkening (OR =1.53,95% CI:1.24-1.89),scalp dermatitis (OR =2.38,95% CI:2.01-2.82) and infra-auricular fissure (OR =1.55,95% CI:1.31-1.85) were positively correlated with the severity of AD (all P < 0.05).Conclusion The severity of AD is associated with patients' region,early onset,itching during sweating,xeroderma,and other concomitant symptoms.
摘要
Objective@#To investigate factors influencing disease severity in children of Han nationality with atopic dermatitis (AD) in China, and to provide scientific evidences for prevention and treatment of AD in children.@*Methods@#From November 2005 to May 2015, data were collected from AD children aged 0-12 years in Department of Dermatology, The First Affiliated Hospital of Anhui Medical University and AD sample collection collaboration network in China. Statistical analysis was carried out with SPSS16.0 software by using univariate and multivariate ordinal logistic regression analysis to investigate factors influencing the severity of AD.@*Results@#A total of 2 620 children with AD were enrolled into the study, including 230 (8.8%) with mild AD, 1 379 (52.6%) with moderate AD and 1 011 (38.6%) with severe AD. As univariate analysis showed, factors influencing the severity of AD included region, early onset, itching during sweating, xeroderma, ichthyosis, palmar hyperlinearity, lichen pilaris, orbital darkening, scalp dermatitis and infra-auricular fissure (all P<0.01) . There were no significant differences in the severity of AD between different genders, patients with and without personal or family atopic history, or between patients with and without a high serum IgE level (all P > 0.05) . Multivariate ordinal logistic regression analysis showed that southern region (OR = 2.10, 95% CI: 1.76 - 2.51) , early onset (OR = 1.32, 95% CI: 1.01 - 1.71) , itching during sweating (OR = 1.70, 95% CI: 1.42 - 2.02) , xeroderma (OR = 2.57, 95% CI: 2.10 - 3.15) , ichthyosis (OR = 1.49, 95% CI: 1.19 - 1.87) , palmar hyperlinearity (OR = 2.03, 95% CI: 1.61 - 2.57) , lichen pilaris (OR = 1.44, 95% CI: 1.17 - 1.78) , orbital darkening (OR = 1.53, 95% CI: 1.24 - 1.89) , scalp dermatitis (OR = 2.38, 95% CI: 2.01 - 2.82) and infra-auricular fissure (OR = 1.55, 95% CI: 1.31 - 1.85) were positively correlated with the severity of AD (all P<0.05) .@*Conclusion@#The severity of AD is associated with patients′ region, early onset, itching during sweating, xeroderma, and other concomitant symptoms.
摘要
Objective To investigate the prevalence of skin diseases in pre-school children aged 0-7 years in cities of China.Methods From November 2014 to April 2015,12 cities were chosen as survey spots,and pre-school children aged 0-7 years served as respondents.A population-based study was conducted,and 40 vaccination clinics and 80 kindergartens were selected by stratified random sampling.A questionnaire survey and dermatological examination were performed by trained dermatologists.Results A total of 20 033 pre-school children received questionnaires,whose age was 2.41 ± 1.82 years (range,0.08-6.83 years).Among these respondents,7 823 children were found to have skin diseases,with the total prevalence of skin diseases being 39.05% (7 823/20 033).Additionally,the prevalence of skin diseases reported in the 12 cities from high to low was as follows:66.96% (612/914,Dalian),56.73% (2 310/4 072,Shanghai),55.49% (556/1 002,Wuhan),49.18% (390/793,Taiyuan),47.16% (316/670,Chengdu),41.93 % (566/1 350,Nanjing),41.03% (318/775,Chongqing),35.98% (240/667,Hefei),33.87% (677/1999,Shenzhen),31.37% (554/1 766,Changsha),23.52% (1 107/4 706,Beijing),13.42% (177/1 319,Shenyang).Totally,40 kinds of skin diseases were investigated,and the 10 most common skin diseases were eczema/infantile eczema/atopic dermatitis (18.71%,3 749/20 033),ichthyosis vulgaris(6.25%,1 253/20 033),lichen pilaris (5.73%,1 148/20 033),diaper dermatitis (5.29%,1 059/20 033),papular urticaria(5.25%,1 052/20 033),hemangioma/vascular malformation (3.86%,774/20 033),pityriasis alba (3.45%,691/20 033),infectious skin diseases (2.59%,519/20 033),urticaria(1.71%,344/20 033)and contact dermatitis (0.5%,100/20 033).Conclusion The total prevalence of skin diseases among pre-school children in cities of China is 39.05%,and eczema/atopic dermatitis is the most common skin disease.
摘要
Objective To study the role of calcitonin gene-related peptide(CGRP)and vasoactive intestinal peptide(VIP)in the pathogenesis of alopecia areata(AA).Methods Radioimmunoassay(RIA)was used to measure the levels of CGRP and VIP in plasma from30patients with AA and20normal controls.Immunohistochemistry was employed to detect the expression of CGRP and VIP in lesions of21patients with AA and16normal scalps.Results①The plasma levels of CGRP in progressing stage of AA(142.63?67.95pg/mL)were significantly lower than those in stable stage of AA(197.33?67.15pg/mL)and in normal controls(188.40?72.95pg/mL).②The plasma levels of VIP in progressing stage of AA(105.94?55.42pg/mL)were significantly lower than those in stable stage of AA(156.86?47.37pg/mL)and in normal controls(176.44?84.70pg/mL).③The expression of CGRP and VIP was significanly decreased in lesions of AA than that in normal scalps.Conclusion These findings indicate that CGRP and VIP may play a role in the pathogenesis of alopecia areata.