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Objective To investigate the effects of ghrelin on proliferation of vascular smooth muscle cells(VSMC)and the expression of mitochondrial fusion 2(Mfn-2)in cultured human aortic smooth muscle cells(HASMCs).Methods HASMCs were cultured in vitro,treated with different concentrations(10-9,10-8,10-7,10-6,10-5 mol/L)ghrelin or 10-6 mol/L ghrelin for different time(0,6,12,18,24 h).Subconfluent HASMCs at passage 4-6 were used in experiments.MTT essay was used to investigate the effect on proliferation of HASMCs.RT-PCR and Western blot were used to analyse the expression of Mfn-2.Results 10-7-10-5 mol/L ghrelin inhibited the proliferation of HASMCs,and the inhibitory effect of concentration of 10-6 mol/L was the most obvious(P<0.01).Ghrelin inhibited the proliferation of HASMCs in 6-24 h,and it reached the peak at 24 h(P<0.01).10-6 mol/L ghrelin significantly increased the expression of Mfn-2 mRNA and protein(P<0.01).The up-regulation of 10-6 mol/L ghrelin on Mfn-2 mRNA and protein expression reached the peak at 18 h(P<0.01).Conclusion Ghrelin might inhibit the proliferation of HASMC by up-regulating the expression of Mfn-2.
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Objective To explore the effect of continuing nursing on the rehabilitation of patients with benign prostatic hyperplasia (BPH) after transurethral resection of prostate (TURP).Methods Toally 128 BPH patients after TURP in our hospital from May 2015 to May 2016 were evenly divided into control group and observation group by random digit table.The patients in the control group were normally guided at discharge and those in the observation group the continuing nursing was done by telephone,WeChat,QQ or family visits for 6 weeks after discharge besides normal guidance.The two groups were compared in terms of self-care ability,treatment compliance,complications and quality of life.Results After the intervention,the scores on health knowledge,self-care ability,self-care responsibility,self-care concept,self-care skills dimension of the observation group were significantly higher than those of the control group.The treatment compliance was significantly higher than that of the control group and the incidences of complications,including pain,hematuria,urinary intontinerce and retentron and infection,constipation were significantly lower than those of the control group.The scores on quality of life,physical function,role function,cognitive function,emotional function and social function score were significantly higher than those in the control group (all P＜0.05).Conclusion The continuing nursing after surgical treatment of benign prostatic hyperplasia patients can be effective in the improvements of patient's self-care ability,establishment of healthy life behavior,reduction of complications,promotion of their rehabilitation and ultimately the improvement of their quality of life.
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Objective To observe the bleeding risk of bivalirudin combined with ticagrelor in patients with acute ST segment elevation myocardial infarction (STEMI) during emergency percutaneous coronary intervention (PCI) .Methods A retrospective a‐nalysis of 458 patients with STEMI who underwent emergency PCI in our hospital was performed .All patients were divided into the bivalirudin group (217 cases) and the standard heparin group(241 cases) according to the anticoagulation scheme during PCI opera‐tion .All patients administered the dual antiplatelet therapy of aspirin 100 mg and ticagrelor 180 mg before surgery .Then ,all pa‐tients were administered dual antiplatelet therapy of aspirin 100 mg once daily and ticagrelor 90 mg twice daily for a long time .The clinical data were analyzed and the bleeding situation within 72 h after PCI was compared between the two groups .Results There were no statistically significant differences in gender ,age ,body mass ,smoking proportion ,occurrence rates of accompanying and complicating diseases ,RBC count ,platelet count ,Hb and PT before PCI between the two groups(P>0 .05) .The bleeding incidence rate in the bivalirudin group was significantly lower than that in the standard heparin group ,and the difference was statistically sig‐nificant (χ2 =8 .455 ,P<0 .05) .Conclusion Compared with standard heparin ,on the basis of ticagrelor use ,giving bivalirudin dur‐ing PCI process can reduce the bleeding risk of patients .
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Objective To know about the post-traumatic stress disorders (PTSD) of women with termination of pregnancy for fetal anomalies (TOPFA),and investigate the relationship of PTSD and the big five personality traits.Methods The general questionnaire,the Impact of Event Scale-Revised (IES-R) and the Big Five Questionnaire were administered to the women with TOPFA in hospital from February 2014 to February 2015.Results The total score of IES-R of 84 cases was 26.20 ± 16.38.The scores of 49 cases(58.3％) were more than 19 and that of 20 cases (23.8％) more than 35.Women who had the first pregnancy had higher level of arousal symptoms,12.84±7.41 vs.9.65±5.53 (t =2.25,P ＜ 0.05).Women whose scores were above 19 had lower adaptation,sociality,altruism and conscience compared with those scored ≤ 19,14.96±3.29,16.84±3.00,18.55±3.49,17.57±2.99 vs.13.57±2.82,18.31±2.65,20.03±2.32,18.86±2.21,t=2.02-2.33,P ＜ 0.05.The IES-R score had no linear correlation with that of the big five personalities,and intrusion subscale had linear correlation with the adaptation.Avoidance symptoms had low positive relationship with sociality in women whose IES-R were above 19,r =0.23,P ＜ 0.05.In populations whose IES-R scored above 19,avoidance subscale had positive weak correlation with sociality,r=0.31,P ＜0.05.Conclusions The women with TOPFA had high level of PTSD before termination.Women with sever posttraumatic stress symptoms had lower sociality and altruism.Moreover,of the women who had sever posttraumatic stress symptoms,one with higher sociality always had higher avoidance symptoms.
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Objective To evaluate the changes of left atrial structure and function in the patients with atrial fibrillation who accepted bi -polar radio frequency current ablation .Methods All patients in our study were divided into three groups including the ones who had accept -ed bipolar radio frequency current ablation because of atrial fibrillation ,the ones who refused to accept ablation operation in despite of atrial fibrillation and the ones who maintained sinus rhythm before operation .All patients accepted TTE before and after operation .Results The rate of transverse diameter change of ablation group (24.24 ±8.67)%was larger than those of other two groups (P<0.05).Left atrial chan-nel capacity volume change rate of ablation group (17.18 ±3.26)%were larger than the values of the group who were suffering atrial fibrilla-tion but refused ablation advice (0.86 ±0.26)(P<0.05).Left atrial total ejection fractions of ablation group (202.41 ±81.59)%were lar-ger than the values of the group who were suffering atrial fibrillation but refused ablation advice (109.53 ±60.91)%( P<0.05) .And its ac-tive ejection fractions(12.18 ±3.48)%,total ejection fractions(41.31 ±8.26)%and so forth were less than the group who maintained si-nus rhythm before operation(21.33 ±5.61)%,(45.05 ±7.37)%,respectively (P<0.05).Left atrial posterior wall center and cardiac basal segment strain(14.34 ±9.47)%,(13.20 ±8.38)%,respectively,and strain rate values after operation of ablation group were less than other segments .Conclusion Left atrial structure remodeling inversion was not obviously 3~6 months after bipolar radio frequency cur-rent ablation .And at the same time ,the improvement of left atrial storage function was not obviously compared with the group who refused ab -lation advice,left atrial channel function compensation degree descended obviously left atrial co -pumping function in ablation group increased obviously,left atrial posterior wall center and cardiac basal segment systolic and distolic function were less than other segments of left .
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Objective To explore the characteristics of cognitive function in patients with the first-episode and chronic schizophrenia. Methods 83 cases of first-episode schizophrenia patients, 77 cases of chronic schizophrenia patients and 75 healthy volunteers from January 2013 to June 2014 were selected. MATRICS consensus cognitive battery (MCCB) test was conducted on them and their cognitive profile patterns and characteristics of cognitive function impairment were analyzed. Results Compared with the healthy control group, the first-episode schizophrenia patients and chronic schizophrenia patients got hurt in psychomotor speed, attention, working memory, verbal learning and memory, visual learning and memory, reasoning and problem solving ability, social cognition and other areas in the MCCB (P<0.05). There were differences in comparing working memory and social cognition of the first-episode schizophrenia patients and chronic schizophrenia patients (P<0.05). Conclusion The first-episode schizophrenia patients get less injury than chronic schizophrenia patients in the working memory and social cognition. Whether first-episode schizophrenia pa-tients or chronic schizophrenia patients, they are impaired compared with the normal population in cognitive function.
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Objective To study the calcium metabolism in tacrolimus(FK506)induced rats nephrotoxicity and the preventive effect of calcium channel blocker.Methods Twenty-four Spragueinduced or FK506-induced nephropathy model.Blood creatinine,blood electrolytes,renal tissue histopathology(HE stain)and the change of ultrastructural organization in renal cells by transmission electron microscope were observed.Results The blood creatinine levels of both CsA and FK506 groups [(36.00±2.61)and(34.17±4.54)μmol/L] were significantly higher than those of the FK506+Dilgroup and control group(all P＜0.05).The blood calcium levels of both CsA and FK506 groups (2.00±0.04 and 2.05±0.04 mmol/L) were significantly lower than those of the FK506+Dil group and control group(all P＜0.05).The blood creatinine and calcium levels of FK506+Dil group were not significantly different with those of control group(P＞O.05).Histopathology examination showed cloudy swelling and vacuolization of the renal tubular epithelial cells and intra-cellular mitochondria swelling and vacuolization in the CsA and FK506 groups.However,the pathological changes of the FK506+Dil group were remarkably milder in comparison with the CsA and FK506 groups.Concluum channel blocker,Dil,could prevent the FK506-induced nephrotoxicity.
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Objective To evaluate the clinical significance of prostatic hypoechoic lesions on transrectal ultrasound (TRUS) for prostate cancer detection.Methods Four hundred and thirty-eight patients referred for biopsy of the prostate from August 1999 to August 2004. Every patient received 6-13 biopsy cores of the prostate. If a hypoechoic was identified, the biopsy was taken from this lesion. Correlation between hypo-echoic lesions, isoechoic areas and cancer detection for each core was performed. Results Among 438 patients, cancer was detected in 112(25.6%). Hypo-echoic lesions were seen in 75 (67%) patients with prostate cancer, the isoechoic were as in 37 (33%). A total of 3504 biopsy cores were obtained from 438 patients, and 636 biopsy cores were taken from hypoechoic lesions. Among 636 biopsy cores, 163 (25.6%) were cancer and 473(74.4%)were not. The cancer detective rate in patients with hypoechoic lesions (75/298, 25.2%) was similar to non-hypoechoic lesions (37/140, 26.4%) on TRUS. There was no statistically significant difference between them (P
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4.00 ng/ml) were divided into BPH group (without inflammation), acute inflammation group and chronic inflammation group. The diagnosis was confirmed by transrectal-ultrasound guided needle biopsies. Prostate volumes were measured in all patients by the transrectal-ultrasound. A total of 126 patients were included in this study. The biopsy results showed 47 patients in BPH group, 45 patients in acute inflammation group and 34 patients in chronic inflammation group. Results The serum PSA value in the BPH group, acute inflammation group and chronic inflammation group were (5.76?3.21),( 8.67 ?2.92 ) and (5.54?3.01) ng/ml respectively. The serum PSA value in acute inflammation group was much higher than that of BPH group and chronic inflammation group(P
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<p><b>OBJECTIVE</b>To determine the efficacy and incidence of complications of extracorporeal shock-wave lithotripsy (ESWL) compared with pneumatic ureteroscopic lithotripsy (URSL) in the treatment of lower uretheral calculi.</p><p><b>METHODS</b>From August 1997 to June 1999, 210 patients with lower ureteric calculi were treated with ESWL and the other 180 with URSL. The stones were fragmented with pneumatic lithotripter. The outcome was assessed by evacuation, retreatment and complication rates.</p><p><b>RESULTS</b>ESWL for lower ureteric calculi resulted in a stone evacuation rate of 78.1%, compared with 93.3% for URSL (P < 0.05). ESWL had a retreatment rate of 11.9% and a perforation rate of 0, while URSL caused perforation of ureters in 3.3% of patients and a refreatment of 2.2%.</p><p><b>CONCLUSION</b>For the management of lower ureteric calculi, ESWL provides a non-invasive, simple and safe option, and URSL has a higher stone evacuation rate but causes ureter perforation more frequently than ESWL does. Both ESWL and URSL have their respective advantages. It is recommended, however, that URSL be extensively developed for better treatment efficacy, given that the operator has an adequate technical background.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Lithotripsy , Methods , Ureteroscopy , Urethral Diseases , Therapeutics , Urinary Calculi , Therapeutics摘要
Purpose To find the reasons and explore the appropriate countermeasures for failure ureteroscopy operation.Methods The clinical data of 74 cases with failure ureteroscopy operation for ureteral calculi were analysed respectively.Results The failure reasons including the difficult of ureteroscopy insert (46 lateral),difficult of calculi disposal (12 lateral) and ureteral injure (15 lateral).ESWL(34 sides) and open surgery(44 sides) were remedial measures after failure ureteroscopy and the success rates were 85 3%, 100% respectively.nephrectomy were carried out in and 1 case died from serious uriaemia.The mortality was only 1.4%.Conclusions The reasons of failure including unable approach the stone, difficult to disposal calculi and ureteral injury. The effect is related to applicances, experience, indications . ESWL and open surgery are the suitable remedy measures according to the failure reason.
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Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK 20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with two step polymerase chain reaction (nested RT PCR) was used to detect CK 20 mRNA expression in the peripheral blood from patients with bladder carcinomas. Results Detection of CK 20 mRNA expression was positive in 37 of 91 patients with bladder carcinoma (41%).Among 20 patients with distant metastasis,17 were positive (85%).CK 20 mRNA was not detectable in the blood samples from 25 normal individuals.The frequency of positive CK 20 mRNA expression was signficantly higher in those with distant metastasis. Conclusions The presence of CK 20 mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells.
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Objective To evaluate the clinical value of transrectal ultrasound guided systematic 13 cores prostate biopsy. Methods A total of 160 patients referred for abnormal digital rectal examination and (or) prostate specific antigen 4ng/ml or greater underwent transrectal ultrasound guided systematic 13 cores prostate biopsy.That was,in addition to the standard sextant biopsies,cores were taken from the far lateral and middle regions of the gland as described by Eskew.Pathological findings of the additional regions were compared with those of the sextant regions. Results Of the patients 35 % had cancer on biopsy(56 /160).Of the 56 patients with prostate cancer 12(21%)had carcinoma only in the additional regions,which would be undetected on the sextant biopsy technique (P
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OBJECTIVE:To study the therapeutic efficacy of Yixinkangtai capsules(YXKT)on climacteric syndrome in males.METHODS:360cases of male climacteric syndrome randomly divided into2groups:trial group(220cases)was treated with YXKT for3wks and control group(140cases)received placebo.RESULTS:In the trial group,the rate of symptomatic improvement was74.3%.Before and after3-week treatment,blood testosterone levels were(131.51?19.12)mg/L and(253.78?21.45)mg/L;SOD levels were(1068?121.4)IU/(g?Hb)and(1178.1?132.6)IU/(g?Hb),MDA levels were(7.6?0.8)?mol/L and(5.8?0.6)?mol/L respectively,and depression score was improved as well.There were significant dif?ferences in all above-mentioned parameters between2groups.CONCLUSION:YXKT is effective and safe for treating cli?macteric syndrome in males.
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OBJECTIVE:To evaluate the therapeutic effect of oral Yixinkangtai(YXKT) on chronic prostatitis METHODS:85 patients with chronic prostatitis were observed for the levels of SOD in saccus prostaticus as well as their subjective symptoms and objective indices before and after taking YXKT to evaluate the therapeutic effect RESULTS:The average level of SOD in saccus prostaticus in patients with chronic prostatitis was (153 76?90 23)mg/L,which was lower than(220 31?85 02)mg/L as in normal males(P