Effect of MANF on proliferation, migration, invasion and apoptosis of colorectal cancer Caco-2 cells / 中国药理学通报

Aim To construct colorectal cancer Caco-2 cell line with overexpression of mesencephalic astrocyte-derived neurotrophic factor (MANF) and to investigate the effects of MANF on the proliferation, migration, invasion and apoptosis of human colorectal cancer Caco-2 cells. Methods Caco-2 cells were transfected with MANF-GFP plasmid and GFP empty vector plas-midrespectively. Real time polymerase chain reaction (RT-PCR) and Westernblot (WB) were used to detect the the expression level of MANF. Transwell was used to detect the effect of MANF on the migration and invasion ability of Caco-2 cells. CCK8 was used to dectet the influence of MANF on the proliferation of Caco-2 cells. WB and flow cytometrywere used to detect the activation ofcaspase-3andthe apoptosis rateof Caco-2 cells. Luciferase experiment was used to verify the effect of MANF on NF-κB pathway. Results MANF-GFP plasmid was successfully expressed in Caco-2 cells. The migration and invasion ability of Caco-2 cells were effectively reduced (P <0. 01), and the a-bility of the cell proliferation was downregulated (P < 0.01) in the MANF overexpression group; the expression of cleaved caspase-3 protein and apoptosis of Caco-2 cells were upregulated; compared with the control group, the transfection of MANF plasmid significantly reduced the transcription activity of NF-κB (P < 0. 01). Conclusions MANF can inhibit proliferation, migration, invasion of Caco-2 cells, while promote its apoptosis by negatively regulating NF-κB signal pathway.

Multiparametric Functional Magnetic Resonance Imaging for Evaluating Renal Allograft Injury

Kidney transplantation is the treatment of choice for patients with end-stage renal disease, as it extends survival and increases quality of life in these patients. However, chronic allograft injury continues to be a major problem, and leads to eventual graft loss. Early detection of allograft injury is essential for guiding appropriate intervention to delay or prevent irreversible damage. Several advanced MRI techniques can offer some important information regarding functional changes such as perfusion, diffusion, structural complexity, as well as oxygenation and fibrosis. This review highlights the potential of multiparametric MRI for noninvasive and comprehensive assessment of renal allograft injury.

Relationship between dyadic coping and intimacy in gynecological cancer patients and partners / 中国心理卫生杂志

Objective:To investigate the dyadic coping and intimacy in gynecological cancer patients and their partners,and to explore the association between the two aspects.Methods:A total of 180 postoperative couples with gynecological cancer from 4 tertiary hospitals were investigated with Dyadic Coping Inventory (DCI) and Short Marital Adjustment (MAT).The relationship between couples,dyadic coping and intimacy were analyzed with the Actor-Partner Interdependence Model (APIM).Results:APIM result showed that in the actor effect patients' and spouses'supportive dyadic coping,delegated dyadic coping and common dyadic coping were positive associated with their own intimacy (B =1.77-4.41),patients'and spouses'negative dyadic coping were both adversely associated with their own intimacy (B =-2.81,-2.66).The partner effect showed that patients' supportive dyadic coping and delegated dyadic coping were both positive associated with spouses'intimacy (B =1.00,4.07),spouses'delegated dyadic coping and common dyadic coping were both positive associated with patients' intimacy (B =2.67,2.60),patients' and spouses'negative dyadic coping were negatively associated with partner'intimacy (B =-1.67,-1.40).Conclusion:It suggests that the dyadic coping may be associated with both patients'and partners' intimacy.