摘要
Objective To analyze the structure and component of gouty tophus around joint, and provide basis to select solvent of gouty tophus and surgical cleaning method.Methods The sample of gouty tophus were obtained from 6 patients in 187 hospital of PLA from January2016 to December 2017, which were rinsed by distilled water, and then dried and accepted other dispose.The sample were analyzed by Fourier Transform Infrared Spectroscopy (FTIR), scanning electron microscope and thermal gravimetric analyzer.Results Sodium urate was the main component of the uratoma, the proportion was 56％;the organic component was about 14％of the uratoma.The microstructure of the uratoma under scanning electron microscope have two forms, the one looks like column crystal and the other like granules head up to lump.Conclusion The main component of the gouty tophus are sodium urate and organic tissues, the possible solvent should react on the both of them.
摘要
<p><b>OBJECTIVE</b>To observe the role of green fluorescent protein (GFP) in tracing rhesus bone marrow stromal cells (rBMSCs) during tissue-engineered bone formation in vivo.</p><p><b>METHODS</b>Ad5.CMV-GFP was amplified by infecting QBI-293A cells, and the bone marrow was harvested from the ilium of adult male rhesus to obtain rBMSCs, which were cultured and passaged in vitro. GFP was transfected into the third-passage rBMSCs via adenovirus vector and the labeled cells were inoculated into absorbable HA scaffold and cultured for 3 days, with untransfected rBMSCs as control, before the cell-matrix compounds were implanted into the latissimus dorsi muscles of rhesus. Samples were harvested at 6 week and embedded in paraform, and ground sections of the bone tissue were prepared to observe green fluorescence under laser scanning confocal microscope. Propidium iodide staining of the sections was also performed for observation.</p><p><b>RESULTS</b>The rBMSCs grew well after GFP transfection, and green fluorescence could be seen 24 h after the transfection and became stronger till 48 h, with a positive transfection rate beyond 80%. Six weeks after cell implantation, the rBMSCs labeled by GFP-emitted green fluorescence were detected in the bone tissue under laser scanning confocal microscope.</p><p><b>CONCLUSION</b>GFP can effectively trace BMSCs during bone tissue engineering, and the transplanted BMSCs constitute the main source of bone-forming cells in bone tissue engineering.</p>
Subject(s)
Animals , Male , Bone Substitutes , Cell Differentiation , Cells, Cultured , Green Fluorescent Proteins , Genetics , Metabolism , Macaca mulatta , Mesenchymal Stem Cells , Cell Biology , Metabolism , Microscopy, Confocal , Tissue Engineering , Methods , Transfection摘要
To improve the antibiotics production of Streptomyces qinlingensis sp. nov.,protoplast regeneration combined with physical and chemical mutagenesis was used to selected high-yielding strains. The results showed that the antibacterial activities of strain R-72 from protoplast regeneration and NTG-1,H30-7 from protoplast mutagenesis against Bacillus subtilis were more than 20% higher than that of the original strain,and the heredity characters of those strains were stable in successive ten generations. The further bioassay experiments exhibited that the fungicidal and antibacterial activities of the fermentation broth from R-72,NTG-1 and H30-7 were remarkable increased comparing with that of the starting strain.