摘要
Objective To compare the diagnostic value of 18F-fluorodeoxyglucose(18F-FDG)positron emission tomography(PET)/CT and MR whole body diffusion weighted imaging(WB-DWI)in malignant tumor bone metastasis.Methods A total of 100 patients with suspected malignant tumor bone metastasis admitted were selected as the research objects.All patients underwent 18F-FDG PET/CT and WB-DWI before treatment.Pathological examination and follow-up results were used as the gold standard,the diagnostic value of the two examination modalities in malignant tumor bone metastasis was analyzed.Results There were 93 patients with bone metastasis diagnosed by gold standard,with a total of 232 lesions.The detection rate of 18F-FDG PET/CT was 92.67％,which was not significantly different from that of WB-DWI(89.66％)(P＞0.05).The sensitivity,positive predictive value and diag-nostic coincidence rate in malignant tumor bone metastasis of 18F-FDG PET/CT were 92.67％,98.62％and 98.71％,respectively,which were significantly higher than 89.66％,97.65％and 94.83％of WB-DWI,but the difference was not significant(P＞0.05).The specificity and negative predictive value of 18F-FDG PET/CT were 82.35％and 45.16％,respectively,which were significantly higher than 70.59％and 33.33％of WB-DWI(P＜0.05).The maximum standardized uptake value(SUVmax)of patients with bone metastasis was significantly higher than that of patients with benign lesions,and the apparent diffusion coefficient(ADC)value was significantly lower than that of patients with benign lesions(P＜0.05).Receiver operating characteristic(ROC)curve showed that the diagnostic value of SUVmax combined with ADC[area under the curve(AUC)=0.912,P＜0.05]was higher than that of single examination.Conclusion 18F-FDG PET/CT is more effective than WB-DWI in the diagnosis of malignant tumor bone metastasis,he combination of the two examinations can improve the diagnostic efficacy for malignant tumor bone metastasis.
摘要
Objective To establish a simultaneous detection of diclofenac sodium, fenbufen and rotundine hydrochloride illegally added in Yinhuang soft capsules by TLC-SERS. Methods The samples to be measured were placed on the silica gel plate, and the thin layer was developed with petroleum ether-ethyl acetate (3:5). Location detection was carried out under 254 nm ultraviolet lamp. Nano-silver colloidal solution was sprayed at each spot of separation according to the optimum conditions, and then qualitative identification was done by TLC-SERS. Results A method for simultaneous determination of diclofenac sodium, fenbufen and rotundine hydrochloride illegally added in Yinhuang soft capsule by TLC-SERS was established. And the minimum detection limits of three chemical constituents were determined. Conclusion TLC-SERS was a rapid, accurate and sensitive method for the simultaneous and rapid detection of diclofenac sodium, fenbufen and rotundine hydrochloride illegally added into Yinhuang soft capsule.
摘要
Objective: To observe the effect of 70% ethanol extract from Huanglian Jiedu Decoction on expression of S180 tumor cell apoptosis factor in MDR model mice, and to research the molecule biology base fot directing clinic. Methods: simulate Clinic PFC project was simulated, the mice model of multi-drug resistance of S180 tumour cell was established. 70% Ethanol extract from Huanglian Jiedu Decoction were given for 10 days. The flow cytometry was used to observe Fas, Trail, CD54.,et al. Resluts: 70% Ethanol extract from Huanglian Jiedu Decoction can increase expression rate of Fas, Trail and improve apoptosis of S180 cell, at the same time it can decrease expression of CD54.Conclusions: 70% Ethanol extract from Huanglian Jiedu Decoction improve high level expression of apoptosis factor, and it maybe one of pass reverse multi-drug resistance of tumour.