Imperatorin is responsible for the vasodilatation activity of Angelica Dahurica var. Formosana regulated by nitric oxide in an endothelium-dependent manner / 中国结合医学杂志

<p><b>OBJECTIVE</b>To demonstrate the vasodilatation activity of the coumarin-containing Angelica dahurica var. formosana and to further analyze active components in the herb extracts.</p><p><b>METHODS</b>(1) The vasodilatation effects induced by different extracts (cyclohexane, ethyl acetate, acetone, methanol, 95 % ethanol and water) of Angelica dahurica var. formosana on mouse thoracic aorta pre-contracted with phenylephrine were investigated. (2) The amount of imperatorin and isoimperatorin in each extract was measured by high-performance liquid chromatography. (3) The vasodilatation effects of imperatorin and isoimperatorin on mouse thoracic aorta were compared using the same in vitro method. (4) The vasodilatation mechanism of imperatorin in the mouse thoracic aorta pre-contracted with phenylephrine was studied using the methods of denuded endothelium, NG-nitro-L-arginine methylester (L-NAME, a nitric oxide synthase inhibitor), and propranolol.</p><p><b>RESULTS</b>(1) The cyclohexane and ethyl acetate extracts of Angelica dahurica var. formosana decreased the maximal response of phenylephrine-induced mouse thoracic aorta contraction dose-dependently, with 50% inhibiting concentration (IC(50)) values of 35.3+/-12.4 mg/L and 40.5+/-12.0 mg/L, respectively. The vasodilatation effect of imperatorin and isoimperatorin was dose-dependent. (2) The cyclohexane extract, showing the strongest vasodilatation effect, possessed the highest contents of imperatorin (4.09%) and isoimperatorin (0.27%, w/w). There was a correlation between the vasodilatation activity and the contents of imperatorin and isoimperatorin in the extracts. (3) The vasodilatation effect of imperatorin was about 4-fold stronger than that of isoimperatorin. (4) The vasodilatation effect of imperatorin was signifificantly attenuated to 24.88%+/-4.06% in the denuded endothelium group compared with the intact endothelium group. And 1 mmol/L L-NAME reduced the imperatorin-induced vasorelaxation by 32.18 %+/-11.29 %.</p><p><b>CONCLUSIONS</b>The principal effective component of Angelica dahurica var. Formosana was found to be imperatorin. Imperatorin-induced vasodilatation is at least partially regulated by nitric oxide, and has no correlation to beta-receptor.</p>

Modified retention elements of removable reverse headgear appliances / 华西口腔医学杂志

<p><b>OBJECTIVE</b>Retention elements were added in the removable reverse headgear appliances in order to achieve better treatment effects of skeletal Class III malocclusion.</p><p><b>METHODS</b>Eighteen patients who needed treatments with reverse headgear appliances were randomly and equally divided into two groups with traditional type of retention elements (T group) and modified type of retention elements (M group) respectively. For the modified type appliances, an adams clasp was added in the position of central incisors on the basis of the traditional type. The minimum dislocating force for both types was measured with the same forcemeter in the first and second visit, respectively. The occurrence of dislocation caused by traction was recorded during the visits of the first three months.</p><p><b>RESULTS</b>The M group showed significantly greater retention than the T group. The minimum dislocating force for M group was larger in the first and second visit (P<0.01, P<0.001). Furthermore, during the first three months, two times of dislocation occurred in M group while it was nine times for T group, indicating great difference in retention (P< 0.05).</p><p><b>CONCLUSION</b>Adding an adams clasp in the position of upper central incisors could effectively prevent the downward dislocation of the appliance when traction force was applied. Accordingly, the therapeutic efficacy was greatly improved.</p>

Hepatitis c virus genotype research by ABC programs of 5'-NCR restriction endonuclease digestion / 中华肝脏病杂志

<p><b>OBJECTIVE</b>In order to fully understand hepatitis c virus (HCV) genotype 3b, 1a, 2b and 6a infection in China, We built HCV 5'-noncoding region (5'-NCR) of different genotypes and subtypes.</p><p><b>METHODS</b>The classification HCV into variable genotypes (subtypes) was carried on by programs A, B and C A. Using a combination of three restriction endonuclease BHH' (BsrB I, Hae II, Hinf I) digestions at the same time. The distinct genotypes were classified into 5 groups: genotype 1 (1a, 1b), 6a, 2 (2a, 2b), genotype 3 (3a, 3b), genotype4 (4a). B. With regard to genotype 1, we could distinguish subtype 1a from 1b using BstU I digestion. C. Using restriction endonuclease Hae III, genotype 2a, 2b, 3b, 4a, 6a are differentiated respectively.</p><p><b>RESULTS</b>(1) HCV genotype 1a, 1b, 2a, 2b, 3a, 3b, 4a, 6a are fully discriminated by comparison with the genotypes regular samples. (2) Of the 93 patients, HCV genotype distribution in China was 66.67% for 1b, 18.28% for 2a, 3.23% for 1b/2b, 3b, 2b respectively. 2.15% for 2a/2b, 1b/2a respectively. 1.08% for 1a.</p><p><b>CONCLUSION</b>This research indicated that adoption of HCV 5'-NCR A B C restriction endonuclease digestions techniques, might be sensitive and efficient to detect HCV and discriminate HCV genotype (subtypes) 1a to 6a.</p>

Comparison of standard large trauma craniotomy with routine craniotormy in treatment of acute subdural hematoma / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To compare the therapeutic effect and indication between standard large trauma craniotomy and routine craniotomy.</p><p><b>METHODS</b>There were 97 patients in the standard large trauma craniotomy group and 110 patients in the routine craniotomy group. The mortality, postoperative ICP (intracranial pressure), ratio of pupil rebound, complication and results of six month follow-up after operation were compared between the two groups.</p><p><b>RESULTS</b>Fifteen patients (15.6%) died in the standard large trauma craniotomy group and 30 (27.7%) in the routine craniotomy group. The postoperative mean ICP was 3.75 kPa+/-1.89 kPa in the standard large trauma craniotomy group and 5.11 kPa+/-1.57 kPa in the routine craniotomy group. The pupil rebound was found in 47 patients (61.0%) in the standard large trauma craniotomy group and in 41 patients (46.1%) in the routine craniotomy group (P<0.01). The rate of complication was lower in the standard large trauma craniotomy group, but no obvious difference in long-term therapeutic effect was found between the two groups.</p><p><b>CONCLUSIONS</b>Standard large trauma craniotomy can attenuate brain hernia and the mortality of the patients with acute subdural hematoma. The incidence of complication can also be decreased. But the long term life quality of the patients can not be improved.</p>