Your browser doesn't support javascript.
loading
節目: 20 | 50 | 100
结果 1 - 14 de 14
过滤器
添加過濾器








年份範圍
1.
文章 在 中文 | WPRIM | ID: wpr-1039631

摘要

ObjectiveTo explore the underlying mechanism by which the Chinese medicine compound Yitangkang granule(YTK) treats diabetic kidney disease (DKD) by observing its effects on podocyte autophagy through the regulation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/forkhead transcription factor O1 (FoxO1) signaling pathway mediated by silent information regulator 1 (SIRT1) via advanced glycation end products (AGE)/receptor for AGE (RAGE) axis. MethodNinety-six 8-week-old healthy male SPF-grade Wistar rats were selected and randomly divided into blank control group (B), model control group, high-dose YTK (40 g·kg-1), medium-dose YTK (20 g·kg-1), low-dose YTK (10 g·kg-1), and Western medicine control (20 mg·kg-1 losartan) groups. The DKD rat model was established by high-fat diet feeding combined with intraperitoneal injection of streptozotocin. After successful modeling, the rats in each group received the corresponding treatments for eight weeks. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and catalase (CAT) were measured according to the instructions of the respective assay kits. Hematoxylin and eosin (HE) staining was used to observe pathological changes in kidney tissues. Immunohistochemistry was employed to detect the average optical density values of α-smooth muscle actin (α-SMA), fibronectin (FN), desmin, and nephrin. Western blot analysis was used to measure the expression levels of PI3K, phosphorylated PI3K (p-PI3K), Akt, phosphorylated Akt (p-Akt), RAGE, SIRT1, Caspase-3, and FoxO1 proteins in kidney tissues of DKD rats. ResultCompared with the blank control group, the model group showed significantly lower levels of SOD, GSH-Px, and CAT, and significantly higher levels of MDA (P<0.01). The rats exhibited severe kidney damage. The positive expression of podocyte marker proteins α-SMA, FN, and desmin increased significantly, while nephrin and podocin significantly decreased (P<0.01). The expression levels of PI3K, p-PI3K, Akt, p-Akt, RAGE, and Caspase-3 proteins were significantly elevated, while SIRT1 and FoxO1 protein levels were significantly reduced (P<0.01). Compared with the model control group, rats in the YTK treatment groups showed significantly higher levels of SOD, GSH-Px, and CAT, and significantly lower levels of MDA in serum (P<0.01). The degree of kidney damage was reduced to varying extents. The average optical density values of podocyte marker proteins α-SMA, FN, and desmin were significantly decreased, while nephrin and podocin significantly increased (P<0.01). The expression levels of PI3K, p-PI3K, Akt, p-Akt, RAGE, and Caspase-3 in kidney tissues were significantly reduced, while SIRT1 and FoxO1 expression levels significantly increased (P<0.01). The Chinese medicine groups demonstrated a clear dose-response trend. ConclusionYTK may alleviate kidney pathological damage, reduce proteinuria, and protect kidney function in DKD rats, thereby delaying the progression of DKD by improving podocyte autophagy through the AGE-RAGE axis-mediated SIRT1 regulation of the PI3K/Akt/FoxO1 signaling pathway. Additionally, a dose-response relationship was observed in the Chinese medicine groups.

2.
文章 在 中文 | WPRIM | ID: wpr-1030750

摘要

ObjectiveTo explore the beneficial role and potential mechanism of intermittent fasting in olanzapine-induced metabolic disorders. MethodsC57BL/6J mice were randomly divided into four groups: Saline + ad libitum (Saline+Ad libitum), Saline + intermittent fasting (Saline +IF), olanzapine administration + ad libitum (Olanzapine+ Ad libitum), and olanzapine administration + intermittent fasting (Olanzapine+IF), with eight mice in each group. The IF group adopted the 5∶2 scheme, that is, fasting on Monday and Thursday every week, and eating freely in the rest of the time. Ad libitum feeding as the control of intermittent fasting, Saline gavage as the control of olanzapine administration. The experiment lasted for 12 weeks. The differences of body mass, liver mass and epididymal adipose tissue mass were compared between the olanzapine-treated group and the control group after IF intervention. The body fat mass, lean body mass, and visceral fat infiltration of mice were analyzed by nuclear magnetic resonance and HE staining, respectively. Furthermore, the levels of fasting blood glucose, insulin, and insulin resistance index (HOMA-IR) in the process of glucose metabolism were also measured by glucose oxidase method and radioimmunoassay, respectively. The effects of IF on H2O2 release and the level of cytochrome C mRNA, a marker related to mitochondrial damage, were detected by ELISA and real-time fluorescence quantitative PCR. ResultsAfter 12 weeks of treatment, olanzapine induced a significant increase in body mass, body fat, lean body mass and visceral fat infiltration (P<0.05), as well as fasting blood glucose, insulin, and HOMA-IR (P<0.05); however, IF significantly reduced the above indicators (P<0.05). Further studies showed that the release of H2O2 and the expression of Cytochrome C mRNA in adipose tissue of mice after intermittent fasting treatment were significantly decreased (P<0.05). ConclusionIntermittent fasting therapy can alleviate olanzapine-induced metabolic disorders in mice. The underlying mechanism may involve the inhibition of oxidative stress level and the maintenance of mitochondrial functions.

3.
文章 在 中文 | WPRIM | ID: wpr-954735

摘要

Intracytoplasmic sperm injection (ICSI) is an important treatment option for male infertility at pre-sent.However, a few patients still suffer from repeated ICSI fertilization failure because their sperm is unable to activate oocytes.Artificial oocyte activation (AOA) technology can improve the fertilization rate, pregnancy rate, live birth rate, etc., but it remains unknown whether AOA has short- and long-term effect on offspring.In this article, recent literature about the effect of AOA technology on perinatal outcomes, genetics, physical development and neurological development of offspring was summarized.This paper aims to provide reference for reproductive medicine workers and pediatricians in clinical practice.

4.
文章 在 中文 | WPRIM | ID: wpr-930435

摘要

Objective:To investigate the effect of oral glucocorticoid dose on maternal and infant outcomes in female patients with systemic lupus erythematosus(SLE), thus providing reference for clinical work.Methods:Clinical data of pregnant women with SLE admitted to Department of Obstetrics, Peking University People′s Hospital from July 2015 to June 2020 were retrospectively analyzed.According to the daily oral glucocorticoid dose during pregnancy(equivalent converted to Prednisone), pregnant women were divided into 2 groups: ≤7.5 mg group(67 cases) and >7.5 mg group(74 cases). The maternal and fetal outcomes of the 2 groups were analyzed by t-test or χ2 test or Fisher′ s exact probability method. Results:The neonatal birth weight, birth length, white blood cell count and platelet count in ≤7.5 mg group were significantly higher than those in >7.5 mg group[(2 990.69±532.20) g vs.(2 734.57±608.35) g, (48.97±2.34) cm vs.(47.43±3.38) cm, (17.69±6.16)×10 9/L vs.(15.11±6.00)×10 9/L, (276.92±74.51)×10 9/L vs.(240.05±69.29)×10 9/L], while the incidence of low birth weight and neonatal transfer rate were significantly lower(13.85% vs.30.43%, 12.31% vs.33.33%) ( t/ χ2=2.587, 3.068, 2.354, 2.841, 5.301, 8.321, all P<0.05). Meanwhile, the incidence of maternal complications like hypertension disorders in pregnancy and gestational diabetes mellitus in the ≤7.5 mg group were lower than those in the >7.5 mg group, but the incidence of anemia and postpartum hemorrhage increased, although no significant differences were detected (all P>0.05). Conclusions:The overall maternal and fetal outcomes of patients with SLE with oral Prednisone ≤7.5 mg/d were better those medicated >7.5 mg/d, but the incidence of anemia during pregnancy and postpartum hemorrhage was higher.An individual monitoring and treatment should be concerned to improve the maternal and infant outcomes.

5.
文章 在 中文 | WPRIM | ID: wpr-907978

摘要

Objective:To investigate the effect of reproduction on maternal and infant outcomes in female patients with systemic lupus erythematosus (SLE).Methods:Clinical data of SLE patients complicated with pregnancy admitted in the Peking University People′s Hospital from July 2015 to December 2019 were analyzed retrospectively.According to the history of live birth, SLE patients complicated with pregnancy were divided into primiparity group (94 cases) and reproduction group (32 cases). Maternal and infant outcomes of the 2 groups were analyzed.Results:A total of 126 SLE patients complicated with pregnancy were included in the present study.The proportion of using immunosuppressants was significantly lower in reproduction group than that of primiparity group [3 cases (9.38%) vs.27 cases (28.72%)] ( χ2=4.927, P=0.026). Complications like lupus nephritis, thyroid disease and postpartum hemorrhage significantly increased in the reproduction group (all P<0.05), and the adverse neonatal outcomes like preterm infants, low birth weight infants and small for gestational age infants in the reproduction group were lower than those in primiparity group, but no significant differences were detected(all P>0.05). There were no significant diffe-rences in the results of the first blood routine examination of newborns and the incidence of neonatal pathological jaundice between the 2 groups (all P>0.05). Conclusions:For SLE patients complicated with reproductive pregnancy, although the incidence of some pregnancy complications increases, the incidence of adverse neonatal outcomes decreases.In addition, reducing the use of immunosuppressants may have positive implications on neonatal outcomes.

7.
文章 在 中文 | WPRIM | ID: wpr-743523

摘要

Objective To investigate the perinatal independent risk factors and neonatal complications of discordant twins.Methods Clinical data of 152 (76 pairs) discordant twins and 552 (276 pairs) concordant twins were enrolled at the Obstetrics of Peking University People's Hospital from November 1,2011 to October 31,2016.The perinatal characteristics and the neonatal complications in 2 groups were analyzed and compared.Binary Logistic regression analysis was used to identify the independent risk factors associated with the occurrence of discordant twins.Results (1) The gestational age,the incidence of term infants and birth weight of the discordant twins were significantly lower than those of the concordant twins[(35.8 ±2.5) weeks vs.(36.7 ± 1.8) weeks,48.7% vs.60.2%,(2 277.1 ± 575.7) g vs.(2 545.0 ± 413.4) g],and the differences were statistically significant (all P < 0.05).The incidence of premature infants,the incidence of infants small for gestational age and the rate of transferring discordant twins to pediatric department were significantly higher than that of concordant twins (51.3% vs.39.9%,40.8% vs.13.2%,46.1% vs.26.8%),and the differences were statistically significant (all P < 0.05).(2) The incidence of neonatal asphyxia,neonatal respiratory distress syndrome,neonatal pneumonia,neonatal apnea,bronchopulmonary dysplasia,neonatal sepsis and neonatal intracranial hemorrhage were significantly higher than those of concordant twins (6.6% vs.2.5%,7.2% vs.3.1%,5.3% vs.1.3%,9.2% vs.3.3%,3.9% vs.0.9%,3.3% vs.0.7%,4.6% vs.O.7%),and the differences were statistically significant(all P < 0.05).(3) The birth weight in the twins with low body weight group was significantly lower than that in the twins with high body weight group [(1 926.7 ± 414.1) g vs.(2 618.7 ± 504.6) g],and the differences were statistically significant (P < 0.05).The incidence of small for gestational age,the rate of conversion to pediatrics and the incidence of neonatal intracranial hemorrhage in the twins with low body weight group were significantly higher than those in the twins with high body weight group (65.8% vs.6.6%,56.6% vs.35.5 %,9.2% vs.0),and the differences were statistically significant (all P < 0.05).(4) Logistic regression analysis showed that hypertensive disorder during pregnancy (OR =2.127,95% CI:1.392-3.253) and gestational diabetes mellitus (OR =1.684,95% CI:1.112-2.552) were independent risk factors for the occurrence of discordant twins.Conclusions Hypertensive disorder during pregnancy and gestational diabetes mellitus are independent risk factors for the occurrence of discordant twins,who are much more likely to develop various neonatal complications,particularly low-birth-weight infants.Timely surveillance and treatment of short-term complications and long-time follow-up are essential to discordant twins.

8.
文章 在 中文 | WPRIM | ID: wpr-923620

摘要

@#Visuo-haptic augmented reality is an active research topic in the field of human-computer interaction, which helps to enhance the patient's realism when it is applied in the field of upper limb rehabilitation. In this paper, several existing visuo-haptic augmented reality systems on rehabilitation were compared and analyzed from the aspects of system composition, interaction modes and applicable objects.

9.
Chinese Journal of Epidemiology ; (12): 821-827, 2017.
文章 在 中文 | WPRIM | ID: wpr-736263

摘要

Objective To conduct a Meta-analysis on the rate of adverse reaction related to rabies vaccine,so as to provide reference for rabies vaccine immunization in China.Methods We electronically searched databases including CNKI,VIP information resource integration service platform,WanFang Data,CBM,PubMed and The Cochrane Library,to collect studies on Chinese people who had received full rabies vaccination and recording all the adverse reactions,from January 2000 to July 2016.Inclusion and exclusion criteria were strictly followed.Meta-analysis for the adverse reaction rate was performed using the R software.Results A total of 29 related papers had met the inclusion criteria,with no publication bias noticed.A total number of 11 020 cases had adverse reactions,among all the 94 222 respondents,with an incidence of adverse reactions as 1.04%-47.78%.The overall incidence rate of adverse reaction was 9.82% (95%CI:7.58%-12.72%).A combined local adverse reaction rate appeared as 12.05% (95%CI:9.26%-15.69%).The systemic adverse reaction rate was 9.06% (95%CI:7.07%-11.61%).The overall adverse reaction rate on aqueous vaccine was 32.39% (95%CI:21.88%-47.94%).Combined adverse reaction rate of freeze dried vaccine appeared as 8.65% (95%CI:4.54%-16.51%).Significant differences were seen between both groups (P<0.05).Conclusions The local adverse reaction rate caused by rabies vaccination was higher than the systemic adverse reaction rate.The adverse reaction rate of aqueous rabies vaccine was higher than that of freeze dried rabies vaccine.Our results suggested that the aqueous vaccine should gradually be eliminated.

10.
Chinese Journal of Epidemiology ; (12): 821-827, 2017.
文章 在 中文 | WPRIM | ID: wpr-737731

摘要

Objective To conduct a Meta-analysis on the rate of adverse reaction related to rabies vaccine,so as to provide reference for rabies vaccine immunization in China.Methods We electronically searched databases including CNKI,VIP information resource integration service platform,WanFang Data,CBM,PubMed and The Cochrane Library,to collect studies on Chinese people who had received full rabies vaccination and recording all the adverse reactions,from January 2000 to July 2016.Inclusion and exclusion criteria were strictly followed.Meta-analysis for the adverse reaction rate was performed using the R software.Results A total of 29 related papers had met the inclusion criteria,with no publication bias noticed.A total number of 11 020 cases had adverse reactions,among all the 94 222 respondents,with an incidence of adverse reactions as 1.04%-47.78%.The overall incidence rate of adverse reaction was 9.82% (95%CI:7.58%-12.72%).A combined local adverse reaction rate appeared as 12.05% (95%CI:9.26%-15.69%).The systemic adverse reaction rate was 9.06% (95%CI:7.07%-11.61%).The overall adverse reaction rate on aqueous vaccine was 32.39% (95%CI:21.88%-47.94%).Combined adverse reaction rate of freeze dried vaccine appeared as 8.65% (95%CI:4.54%-16.51%).Significant differences were seen between both groups (P<0.05).Conclusions The local adverse reaction rate caused by rabies vaccination was higher than the systemic adverse reaction rate.The adverse reaction rate of aqueous rabies vaccine was higher than that of freeze dried rabies vaccine.Our results suggested that the aqueous vaccine should gradually be eliminated.

11.
文章 在 中文 | WPRIM | ID: wpr-453768

摘要

Objective To explore the differences in maternal and neonatal complications of twin pregnancies in different modes of conception.Methods Retrospective analysis was performed to compare the differences in mother's gestation history,childbirth and neonatal complications among 102 cases of in vitro fertilization-embryo transfer(IVFET) pregnancy twins,16 cases of simple ovulation induction(OI) pregnancy twins and 96 cases of spontaneous conception(SC) pregnancy twins.Results 1.The maternal age[(32.51 ±4.03) years vs (31.25 ±3.38) years vs (30.20 ± 4.13) years,F =8.061,P < 0.05] and birthrate of full-term infants (21.5 % vs 0 vs 10.4%,x2 =7.566,P < 0.05) of IVF-ET group were significantly higher than that of OI group and SC group.The body weight at birth of OI group was significantly lower than that of IVF-ET group and SC group [(1 918.1 ±516.4) g vs (2 228.3 ±4542) g vs (2 164.4 ±442.5) g,F =3.293,P <0.05].The birthrate of late preterm infant(87.5% vs 51.0% vs 64.6%,x2 =9.322,P < 0.05) and the incidence of twin discordance (50.0% vs 21.6% vs 18.8%,x2 =7.781,P < 0.05) of OI group were significantly higher than that of IVF-ET group and SC group,while the incidence of monozygotic twins of SC group was significantly higher than that of IVF-ET group and OI group(52.1% vs 5.9% vs 0,x2 =58.016,P < 0.05).2.The incidence of pregnancy induced hypertension of OI group was significantly higher than those of IVF-ET group and SC group(50.0% vs 17.6% vs 22.9%,x2 =8.344,P < 0.05).The incidence of gestational diabetes mellitus of OI group and spontaneous group was significantly higher than that of IVF-ET group(12.5% vs 14.6% vs 3.9%,x2 =6.838,P <0.05).3.The incidence of twin transfusion syndrome of SC group was significantly higher than that of the other groups(6.3% vs 0 vs 0,x2 =7.588,P <0.05),but the incidence of apnea of prematurity of OI group was significantly higher than that of IVF-ET group and SC group(43.8% vs 15.7% vs 24.0%,x2 =7.081,P <0.05).The incidence of asphyxia,transient tachypnea of the newborn,hypoglycemia,intracranial hemorrhage and septicemia of OI group were higher than that of IVF-ET group and SC group,but there was no significantly difference among 3 groups.Conclusions IVF-ET conceived twin pregnancies are not at greater risk for pregnancy complications and adverse perinatal outcome than SC twin pregnancies.But the incidence of some adverse maternal and neonatal complications of simple OI twin pregnancies is higher than that of IVF-ET group and SC group.More attention should be paid to the perinatal health care of OI conception twins,which is important to avoid serious complications.

12.
Acta Anatomica Sinica ; (6): 578-581, 2014.
文章 在 中文 | WPRIM | ID: wpr-455086

摘要

Objective To study the characteristics of skinfold thickness of Han adults in Jiangsu province . Methods The skinfold thicknesses of facial , subscapular , suprailiac , biceps , triceps and calf on 311 urban adults ( 157 males and 154 females) and 421 rural adults ( 213 males and 208 females ) of Han were investigated in Huaian city of Jiangsu province .Results The thickness of skinfold of urban females were thicker than that of urban males .Rural adults were the same .Han adults of Jiangsu showed the most significant differences between urban areas and rural areas .The values of six skinfold thicknesses of Jiangsu urban adults have positive correlation with age .Conclusion Han adults of Jiangsu show the most significant differences between genders .

13.
Military Medical Sciences ; (12): 392-395, 2014.
文章 在 中文 | WPRIM | ID: wpr-451478

摘要

Sulfur mustard [bis (2-chloroethyl) sulfide, sulfur mustard, SM] is considered a powerful chemical warfare agent.There is still no specific antidote due to its complex toxicological mechanism .An intimate knowledge of the toxic mechanisms and pathophysiological changes is important to the treatment of sulfur mustard injury .Oxidative stress is one of the most important pathophysiological processes involved in the toxic effect of sulfur mustard .The study of oxidative stress biomarkers induced by sulfur mustard can help to reveal the role of oxidative stress in sulfur mustard intoxication , which may contribute to better understanding of the toxic mechanism and development of therapeutic measures after sulfur mustard exposure.The research advances in oxidative stress biomarkers in sulfur mustard intoxication are reviewed .

14.
Hamdard Medicus. 1996; 39 (3): 102-103
在 英语 | IMEMR | ID: emr-41092
搜索明细