摘要
Objective To investigate the effects of self-made carriers on the cryopreservation of ovarian tissue of sheep. Methods Thirty-two ovaries were randomly assigned to fresh group,programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group.The morphology,proliferation,apoptosis,and estrogen level of the ovarian tissue in each group were observed. Results After cryopreservation,the morphology normal rate of the primordial follicles in programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group were 74.2%,72.8%,and 72.3%,respectively,lower than that(83.7%)in the fresh group(χ
Subject(s)
Animals , Female , Cryopreservation , Freezing , Ovarian Follicle , Ovary , Sheep , Vitrification摘要
The age of onset of gynecological malignant tumors tends to be younger,more and more patients have fertility requirements when they are diagnosed with cancer.Different cancer treatments have a certain impact on the fertility of patients, application of assisted reproductive technology(ART)can help part of the patients fulfill their fertility wish. In this paper, the methods and safety of ART for three gynecological malignant tumors are discussed.