摘要
Neutralizing capacity measurement (NCM) of soluble ABH substances (SAS) in plasma was assessed to guide the selection of the appropriate ABO group of fresh-frozen plasma (FFP) for plasma exchange (PE) in blood group O recipients with ABO-incompatible transplantations. Neutralizing capacity was assessed by measuring anti-A and/or anti-B titers in samples comprising one unit of O FFP and 10 O EDTA plasma samples and subtracting the binary logarithm of the titer in each group with a saline dilution. Ten EDTA plasma samples with Lewis b (Leb ) antigen positivity and 10 sets of pooled FFP from each blood group were used as diluents. In O FFP, the NCM values (mean ± SD) were 3.4 ± 0.52 (2.6 ± 0.52) and 2.6 ± 0.52 (1.5 ± 0.3) in B and AB for IgM (total antibody) anti-B (both P < 0.001), and in the 10 O EDTA plasma samples, they were 3.9 ± 0.88 (3.1 ± 0.88) and 3.2 ± 0.79 (2.4 ± 0.97) for IgM (P = 0.0013) and total anti-B (P = 0.025), respectively. In vitro analysis revealed that B FFP is more effective than AB FFP in reducing IgM and total anti-B antibody titers in O recipients, regardless of Le b antigen positivity.
摘要
Background@#Maintaining optimal blood inventory levels in hospitals is important to prevent blood shortage and wastage. We aimed to provide an efficient blood inventory management strategy for hospital blood banks nation-wide by comparing the current use of 5-day issuable stock (IS) with Lim’s IS as a novel target IS. @*Methods@#The average and CV of daily usage (DU) were calculated from information entered into Korea’s Blood Management System by 194 participating hospitals in 2019 and 2020. Using these data, Lim’s IS was calculated by determining the simulated annual average blood shortage day nearest to 1 for each blood group in each hospital. The 5-day IS (5IS) was estimated by multiplying the average DU in 2018 by five to count the shortage days in 2019. @*Results@#The average DU (0.3–231.3 units) and corresponding CV (0.33–7.14) in the participating hospitals were inversely proportional (r = –0.699 to –0.695). The hypothetical averages of 5IS and Lim’s IS were 27.0 ± 41.2 and 24.7 ± 20.8, respectively (P = 0.006). The shortage days for 5IS and Lim’s IS were 8.9 ± 22.7 and 1.0 ± 1.9, respectively (P < 0.001). @*Conclusions@#While 5IS was unacceptable for universal application, Lim’s IS remained near one shortage day and is considered more efficient than 5IS. Hospitals should implement indicators that consider DU and its variations. This is the first study to introduce Lim’s IS as an indicator of optimal blood inventory, and the data are expected to provide guidance for effective blood inventory management nationwide, particularly during blood shortages.
摘要
The Valeri method, introduced in 1972 with dimethyl sulfoxide (DMSO) as a cryoprotective agent, has evolved into a no-wash technique widely employed for cryopreservation of platelets. Cryopreserved platelets (CP) are a viable alternative to liquid platelets (LP) and address the limitations of LP. CP have an extended shelf file, are a reliable supply for rare blood types, and can be transported to challenging terrains for use in military settings and remote areas and places where blood supply is imbalanced. Despite CP exhibiting a lower recovery rate compared to LP, the superior hemostatic efficacy makes it advantageous for use in bleeding patients. Some countries have already implemented CP for civilian use in disasters caused by natural hazards or human-induced events, and clinical trials are underway to expand applications among civilians. The absence of national regulations and standardized guidelines for CP preparation and evaluation is a significant obstacle to the extensive use of CP. A consensus is needed among academic societies, blood centers, the military, and governments to lend support and interest in the development of CP as a viable alternative to LP. This review presents information on the initial attempts to produce CP, in vitro changes of thawed CP, utilization of CP, and the current usage status in various countries. The goal was to evaluate the potential need to introduce CP domestically and provide insights on the strengths and challenges.
摘要
Background@#The study aimed to review the initiatives and activities of the Korean Blood Safety Project Group (KBSPG) from 2012 to 2021 to present its work domestically and internationally, and to set up future directions for the KBSPG. @*Methods@#Details of the KBSPG’s projects were organized by task. This project was supported by the Korean Ministry of Health and Welfare. The final annual reports and data posted on the website of the KBSPG were used for analysis. @*Results@#The KBSPG completed 14 tasks, including training and sharing of the latest information with workers of blood centers and transfusion services in medical institutes, external proficiency surveys for donor blood screening tests, and maintenance of a national registration system. The results of these tasks have contributed to the fulfillment of work of the relevant national departments, the establishment and operation of related websites, the publication of annual reports, the implementation of workshops and training programs, the operation of regional networks for blood transfusion management, and the production and distribution of information leaflets and training videos.Additionally, the KBSPG has developed and revised guidelines and standards and published related papers. @*Conclusion@#The KBSPG’s continuous efforts improved the quality of blood centers and transfusion services in medical institutes, thus contributing to the national blood management services. To further improve blood management work and ensure blood safety, the KBSPG must expand its scope and continue to pursue its important mission. (Korean J Blood Transfus 2023;34:1-11)
摘要
Background@#ABO Ab titration using Erytra Eflexis (Diagnostic Grifols, Barcelona, Spain, abbreviated as Eflexis) and a pretransfusion crossmatching using DG Gel Coombs Card (Diagnostic Grifols, abbreviated as Card) were compared with the tube test (TT) to help laboratories. @*Methods@#Samples subjected to TT were titrated for ABO antibody using the Eflexis. The titers obtained were thereafter converted into log steps of 2 (n=20 for each ABO group). The reference range (RR) of the ABO Ab titer using Eflexis was determined using samples from health checkup patients (n=30 for each ABO group). The Card and LISS-antihuman globulin (AHG) test of TT were compared for 40 cases with identified antibodies. @*Results@#The average titers of IgM anti-B of blood group A by TT (3.2 vs 2.4, P＜0.001), and anti-A of type O by Eflexis (4.5. vs 5.5, P=0.001 for IgM, 6.3. vs 7.3, P＜0.001 for total antibody) were higher compared to the alternative method. The RRs of IgM (total antibody) of anti-B and anti-A were determined to be 1:2≤ (1:2≤) for anti-B in group A, 1:8≤ (1:8≤) for anti-A in group B, and 1:8≤ (1:32≤) and 1:16≤ (1:32≤) for anti-B and anti-A in group O, respectively. A comparison of crossmatching revealed that the average hemagglutination strength of the Card was 1.65, which was stronger than the LISS-AHG of 0.50 (P＜0.001). @*Conclusion@#The results of this study are expected to provide valuable insights for the establishment and guidance of laboratory tests utilizing Eflexis and Card.
摘要
Background@#Since there is a paucity of red blood cells (RBCs) with a weak expression of A, B, or D antigens, they cannot be used as routine Blood Bank quality control materials. This study aimed to prepare glutaraldehyde-treated RBCs to be weakened ABO and RhD antigen expressions for blood typing. @*Methods@#RBCs from A1B RhD positive blood group donors were treated with 0.5%, 0.375%, 0.3125%, 0.25%, and 0.125% concentrations of glutaraldehyde. The treated RBCs were then used for ABO and RhD blood typing using the tube technique. Differences in agglutination reaction strength and potassium concentrations were compared using treated RBCs stored days for 0, 1, 4, and 7 days at 2℃–8℃. @*Results@#With an increase in the glutaraldehyde concentrations, the strength of agglutination reaction against anti-A, anti-B, anti-D, and anti-A1 reagents reduced. Most tests showed 2+ strength with 0.25% glutaraldehyde treated RBCs. Hemolysis increased with increasing storage time; however, there were no significant differences in strengths until the 7th day of storage. @*Conclusions@#The preparation of 0.25% glutaraldehyde-treated RBCs is simple, and it can be used as a cheap ABO subtype or D variant quality control material. The use of these treated red cells may improve the accuracy of blood typing in Blood Banks.
摘要
This report summarizes the 2019 survey results of the external quality assessment (EQA) scheme for the Transfusion Medicine Program (TMP) in Korea. Proficiency testing materials were prepared at the Asan Medical Center for the biannual distribution to participating laboratories. The average accuracy rates and number of participants (in parenthesis) for ten survey items were as follows: ABO typing, 99.4%–99.9% (N=875); RhD typing, 99.8%–100% (N=864); crossmatching, 90.8%–99.6% (N=760); ABO subtyping, 98.2% and 100% (N=58); Rh CcEe antigen testing, both 100% (N=55); weak D test, 97.9%–100% (N=232); antibody screening, 99.7%– 100% (N=316); direct antiglobulin test (DAT) using a polyspecific reagent, 99.6%–100% (N=273); DAT using an immunoglobulin-G monospecific reagent, both 100.0% (N=67); DAT using a C3d monospecific reagent, 95.6%–98.5% (N=67); antibody identification, 87.9%–99.2% (N=132); and ABO Ab titration, 85.7%–100% (N=134). The number of participants showed an average increase of 14% across the ten survey items, with the ABO antibody titration showing the highest increase at 83.6%. While results were generally excellent, antibody identification and ABO antibody titration results showed room for improvement. The 2019 EQA scheme for TMP has contributed to the improvement and maintenance of the participating laboratories to the program.
摘要
Background@#The Korean Blood Safety Commission established the Regional Networks for Blood Transfusion Management (RNBTM) Project which has operated in 14 regions across the country since 2017 to help blood transfusion management in small and medium-sized medical institutions. Since implementation of the RNBTM, participant’s satisfaction has not been evaluated, therefore in order to evaluate participant’s satisfaction and assist in future planning a survey was conducted. @*Methods@#Fourteen facilitators participated in an anonymous on-line survey (5 questions). Laboratory Medicine (LM) doctors from small and medium-sized medical institutions from 14 regions also participated in a separate anonymous on-line survey (15 questions). @*Results@#14/14 (100%) facilitators responded to the survey. In addition to the RNBTM they were also in favor of establishing a Network of University Hospitals. 135 LM doctors responded to the survey. Of the RNBTM participants, 94.1% (111/118) replied that RNBTM was helpful in providing and exchanging blood transfusion information. Respondents indicated that they wished to remain part of RNBTM into the future because they felt that RNBTM not only helped improve quality of blood transfusions but also other aspects in the laboratory. In 70.4% (95/135) of participating medical institutions, LM doctors have been the Chairperson of the Hospital Transfusion Committee. @*Conclusion@#Extremely positive survey results were received from LM doctors. These survey results from LM doctors and facilitators will be helpful in developing and planning future projects related to RNBTM.
摘要
Blood is an essential medical resource for treating diseases and trauma of people, but a limited biological resource for which no artificial production is possible. Therefore, systematic and rational management of its supply and use must be carried out under the national responsibility. On the other hand, the low birthrate and aging population have raised the problem of blood shortages in Korea. To help solve this problem, this study examined proposals for the development of a blood management system in Korea. In addition, the works of blood management bodies were analyzed based on the operation subjects and types of blood projects in the United States, the United Kingdom, France, Germany, the Netherlands, Canada, Australia, Singapore, and Japan. Based on these data, this paper proposes a new organization that can best enhance the specialization of national blood services and strengthen its capacity under the current blood supply system in Korea.
摘要
The scientific interest in transfusion medicine as related to COVID19 can be summarized in three important points. 1) How can we identify asymptomatic COVID19 infected potential blood donors from healthy donors, and if the asymptomatic COVID19 infected person has donated blood and it has been transfused, how will it affect the transfused patient? 2) What affect will COVID19 have on blood establishments and medical institutions that use blood? 3) How will convalescent plasma from recovered COVID-19 patients be collected and then be used for patients in need of it? Since COVID19 has a negative effect on blood transfusion and blood management, well developed lines of communication and cooperation from blood establishments, medical institutions, government agencies, and people are urgently needed to overcome the impact of this negative effect.
摘要
Background@#Nurses play a central role in the process of blood transfusion because they have the closest interactions with patients. Providing nurses with the appropriate knowledge and competency through education can help ensure transfusion safety. This study investigated the effectiveness of the first nationwide transfusion-related education for nurses by analyzing questionnaires for a self-assessment of competency on transfusion-related knowledge before and after the education and general evaluation for the educational program. @*Methods@#The education program was composed of four lectures and was conducted in seven regions in South Korea. One hundred and ninety-two nurses participated, and the questionnaires for 170 nurses were analyzed. @*Results@#The participants consisted of 90 nurses (53.0%) from tertiary hospitals, 23 (13.5%) from general hospitals, and 57 (33.5%) from other hospitals. The majority of the participants (103/170, 60.6%) were from hospitals with ≥500 beds, and 69.4% had a work period of ≥10 years. The scores for pre-/post-education self-assessment of competency were as follows: blood components, 3.03/3.73; pretransfusion testing, 2.86/3.64; management of transfusion, 3.18/3.84; and transfusion reactions, 3.11/3.78. In all categories, there was a significant increase in the score after the education program. The majority of participants (99.4%) provided a positive response regarding the necessity of a transfusion-related education program. @*Conclusion@#South Korea’s first attempt at transfusion-related education for nurses showed a strong positive effect by improving participants’ transfusion-related competency. Considering the important role of nurses in blood transfusion, the educational program should continue and be expanded in the future.
摘要
Background@#The current Korean Blood Inventory Monitoring System (BMS) has several drawbacks. It does not provide real-time data and only monitors 211 of the more than 2500 institutes that performed blood transfusions.This survey study investigated the status of BMS use and the preparation status of the data input system of medical institutions to help in the revision of BMS in preparation for the full surveillance of BMS. @*Methods@#A survey questionnaire was given to 200 hospitals participating in the current BMS, along with another set of questionnaires to 150 non-participating hospitals. The questionnaire consisted of the method of data registry to BMS, the current status of electronic medical records (EMR) and order communication system (OCS), perception, and readiness of adaptation of an open application programming interface system (API). @*Results@#Two hundred and one BMS participating hospitals responded to the survey. Approximately 75% entered data with a comma-separated value (CSV) or Excel spreadsheet (xls) files, and approximately half had an in-house developed EMR and OCS. The majority showed enthusiasm for the introduction to an open API. Among the non-participating hospitals, 138 responded. Approximately 70% counted the blood inventory daily, but approximately half did not use electronic methods for this process. The response to the introduction to an open API was positive, but the readiness for a prompt introduction was low at 15.9%. @*Conclusion@#These results will help revise the current BMS. On the other hand, full surveillance of BMS is anticipated to be hindered by the ready state of each medical institute. Moreover, the encouragement of participation would require supportive government administrative measures.
摘要
Background@#Nurses play a central role in the process of blood transfusion because they have the closest interactions with patients. Providing nurses with the appropriate knowledge and competency through education can help ensure transfusion safety. This study investigated the effectiveness of the first nationwide transfusion-related education for nurses by analyzing questionnaires for a self-assessment of competency on transfusion-related knowledge before and after the education and general evaluation for the educational program. @*Methods@#The education program was composed of four lectures and was conducted in seven regions in South Korea. One hundred and ninety-two nurses participated, and the questionnaires for 170 nurses were analyzed. @*Results@#The participants consisted of 90 nurses (53.0%) from tertiary hospitals, 23 (13.5%) from general hospitals, and 57 (33.5%) from other hospitals. The majority of the participants (103/170, 60.6%) were from hospitals with ≥500 beds, and 69.4% had a work period of ≥10 years. The scores for pre-/post-education self-assessment of competency were as follows: blood components, 3.03/3.73; pretransfusion testing, 2.86/3.64; management of transfusion, 3.18/3.84; and transfusion reactions, 3.11/3.78. In all categories, there was a significant increase in the score after the education program. The majority of participants (99.4%) provided a positive response regarding the necessity of a transfusion-related education program. @*Conclusion@#South Korea’s first attempt at transfusion-related education for nurses showed a strong positive effect by improving participants’ transfusion-related competency. Considering the important role of nurses in blood transfusion, the educational program should continue and be expanded in the future.
摘要
Background@#The current Korean Blood Inventory Monitoring System (BMS) has several drawbacks. It does not provide real-time data and only monitors 211 of the more than 2500 institutes that performed blood transfusions.This survey study investigated the status of BMS use and the preparation status of the data input system of medical institutions to help in the revision of BMS in preparation for the full surveillance of BMS. @*Methods@#A survey questionnaire was given to 200 hospitals participating in the current BMS, along with another set of questionnaires to 150 non-participating hospitals. The questionnaire consisted of the method of data registry to BMS, the current status of electronic medical records (EMR) and order communication system (OCS), perception, and readiness of adaptation of an open application programming interface system (API). @*Results@#Two hundred and one BMS participating hospitals responded to the survey. Approximately 75% entered data with a comma-separated value (CSV) or Excel spreadsheet (xls) files, and approximately half had an in-house developed EMR and OCS. The majority showed enthusiasm for the introduction to an open API. Among the non-participating hospitals, 138 responded. Approximately 70% counted the blood inventory daily, but approximately half did not use electronic methods for this process. The response to the introduction to an open API was positive, but the readiness for a prompt introduction was low at 15.9%. @*Conclusion@#These results will help revise the current BMS. On the other hand, full surveillance of BMS is anticipated to be hindered by the ready state of each medical institute. Moreover, the encouragement of participation would require supportive government administrative measures.
摘要
Herein, we report the results of the 2018 survey on the external quality assessment (EQA) scheme for the Transfusion Medicine Program (TMP) in Korea. The proficiency testing specimens were prepared at Ajou University Hospital and were sent to the participants biannually. The average accuracy rates for ten different test items on the regular survey were as follows: ABO typing, 99.5%–99.8% (N=841); RhD typing, 99.8%–100.0% (N=827); crossmatching, 89.4%–99.6% (N=708); ABO subtyping, 94.2% and 94.4% (N=53); Rh CcEe antigen testing, all 100.0% (N=51); weak D test, 80.9% (N=207) for the first trial and not graded for the second trial; antibody screening, 99.7%–100.0% (N=304); direct antiglobulin test (DAT) using a polyspecific reagent, 98.9%–100.0% (N=264); DAT using an immunoglobulin-G monospecific reagent, all 100.0% (N=66); DAT using a C3d monospecific reagent, 97.0%–100.0% (N=67); antibody identification, 98.4%–100.0% (N=127); and ABO antibody titration, 84.6%–100.0% (N=73). There were approximately 10.5% more participants in the 2018 EQA scheme than that in 2017. Excellent survey results were obtained in the 2018 EQA scheme compared with those in 2017, except for the weak D test. The 2018 EQA scheme for the TMP should be helpful for improving the quality of the participating laboratories.
Subject(s)
Coombs Test , Korea , Laboratory Proficiency Testing , Mass Screening , Quality Improvement , Thymidine Monophosphate , Transfusion Medicine摘要
BACKGROUND: Currently there is no external proficiency testing program for allergen-specific immunoglobulin E assays (sIgE) in Korea. The aim of this study was to evaluate the accuracy of sIgE performed in different laboratories in Korea using Polycheck Allergy Diagnostic (Biocheck GmbH, Germany) or simply ‘Polycheck’ and to further perform an inter-laboratory comparison of sIgE. METHODS: Three inter-laboratory trials were undertaken from June 2016 to December 2017 at nine institutions. Serum samples obtained from three patients who had a history of positive Polycheck results were used in each trial. A total of 59 allergens were evaluated from the standard panel, inhalation panel, and food panel. The acceptable rates (%) of classes 0–6 of the results were calculated using three different criteria, including the consensus of 80% participants of the College of American Pathologists (CAP), the most frequent value (MFV), and the Overall Misclassification Index Score (OMIS). RESULTS: The proportion of laboratories that showed acceptable results for CAP criteria, MFV, and OMIS were around 93.0%–97.7%, 66.3%–76.0%, and 0.1–1.3, respectively, in each trial. Under-performing institutes showed consistently higher (49/68, 72.1%) or lower (19/68, 27.9%) than acceptable results for all criteria. CONCLUSIONS: This is the first study evaluating the inter-laboratory variation of sIgE in Korea using various criteria. It was found that the acceptable rate could be altered based on changes in these criteria. These findings might be useful for the inter-laboratory comparison of sIgE in Korea in the future as well.
Subject(s)
Humans , Academies and Institutes , Allergens , Consensus , Hypersensitivity , Immunoglobulin E , Immunoglobulins , Inhalation , Korea , Laboratory Proficiency Testing , Mass Screening , Quality Control摘要
Here, we have reported results of the surveys on the external quality assessment scheme (EQA) of the Transfusion Medicine Program (TMP) in Korea that were carried out in 2017. The proficiency testing specimens were prepared at Ajou University Hospital, and sent to the participants biannually. The average accuracy rates (N=the number of participants) for ten different test items on the regular survey were as follows: ABO typing, 99.1%–99.9% (N=714); RhD typing, 99.3%–100.0% (N=695); crossmatching, 88.9%–98.5% (N=618); ABO subtyping, 80.7% and 96.0% (N=51); Rh CcEe antigen testing, 98.8%–100.0% (N=51); weak D test, 99.3% and 100.0 (N=150); antibody screening, 98.6%–100.0% (N=295); direct antiglobulin test (DAT) using a poly-specific reagent, 99.2%–100.0 (N=256); DAT using an immunoglobulin-G monospecific reagent, all 100.0% (N=68); DAT using a C3d-monospecific reagent, 83.6%–100.0% (N=72); antibody identification, 88.7%–99.2% (N=123); and ABO Ab titration, 84.6%–100.0% (N=73). The number of participants for the EQA for TMP in 2017 was much higher than that in 2016. Except for the case of ABO subtyping, excellent survey results for the 2017 EQA for TMP were obtained, compared to those in 2016. Thus, the EQA for TMP in 2017 should be helpful for improving the quality of the participating laboratories.
Subject(s)
Coombs Test , Korea , Laboratory Proficiency Testing , Mass Screening , Quality Improvement , Thymidine Monophosphate , Transfusion Medicine摘要
BACKGROUND: The management of red blood cell inventory in hospital's blood bank is crucial. The Australian Red Cross Blood Service developed a RBC safety stock calculation method (abbreviated as the ‘Australian formula’). In this study, we applied this method to four Korean hospitals to calculate the safe RBC stock level. METHODS: The hospitals included in this study were three tertiary teaching hospitals and one teaching hospital. The number of hospital beds in these hospitals were 1093, 1330, 1400, and 854, respectively. The data were collected from the Korea Blood Inventory Monitoring System of Centers for Disease Control & Prevention. The target/minimal/maximal RBC inventory levels and inventory days (inventory level/average daily usage) by ABO blood types were calculated using the daily red cell transfusion, wastage, and supply data between May and October 2016. RESULTS: The enrolled hospitals showed different levels for the target/minimal/maximal RBC inventory according to each blood group. The average of RBC inventory days in the four hospitals was 4.2 days. For each blood group, RBC inventory days were 3.2~4.4 days for O blood group type, 3.5~4.7 days for A blood group, 3.9~4.5 days for B blood group, and 3.9~5.5 days for AB blood group. CONCLUSION: Because the optimal RBC inventory levels are different depending on the hospital characteristics and the ABO blood group, it is necessary to set the RBC inventory levels for each hospital distinctly. The data obtained in this study will help manage blood product inventory in various hospital blood banks.
Subject(s)
Blood Banks , Erythrocytes , Hospitals, Teaching , Korea , Methods , Red Cross摘要
BACKGROUND: A massive blood transfusion (MT) requires significant efforts by the Blood Bank. This study examined blood product use in MT and emergency O Rh Positive red cells (O RBCs) available directly for emergency patients from the Trauma Center in Ajou University Hospital. METHODS: MT was defined as a transfusion of 10 or more RBCs within 24 hours. The extracted data for the total RBCs, fresh frozen plasma (FFP), platelets (PLTs, single donor platelets (SDP) and random platelet concentrates (PC)) issued from Blood Bank between March 2016 and November 2017 from Hospital Information System were reviewed. SDP was considered equivalent to 6 units of PC. RESULTS: A total of 345 MTs, and 6233/53268 (11.7%) RBCs, 4717/19376 (24.3%) FFP, and 4473/94166 (4.8%) PLTs were used in MT (P < 0.001). For the RBC products in MT and non-MT transfusions, 28.0% and 34.1% were group A; 27.1% and 26.0% were group B; 37.3% and 29.7% were group O, and 7.5% and 10.2% were group AB (P < 0.001). The ratios of RBC:FFP:PLT use were 1:0.76:0.72 in MT and 1:0.31:1.91 in non-MT (P < 0.001). A total of 461 O RBCs were used in 36.2% (125/345) of MT cases and the number of O RBCs transfused per patient ranged from 1 to 18. CONCLUSION: RBCs with the O blood group are most used for MT. Ongoing education of clinicians to minimize the overuse of emergency O RBCs in MT is required. A procedure to have thawed plasma readily available in MT appears to be of importance because FFP was used frequently in MT.
Subject(s)
Humans , Blood Banks , Blood Platelets , Blood Transfusion , Education , Emergencies , Hospital Information Systems , Plasma , Tertiary Healthcare , Tissue Donors , Trauma Centers摘要
We report here the 2016 results of surveys on an external quality assessment (EQA) for immunohematology testing in Korea carried out in 2016. The proficiency testing specimens were prepared at Ajou University Hospital, and the specimens were sent to participants biannually. The average accuracy rates (N=the number of participants) for ten different test items on the regular survey were as follows: ABO typing, 98.9%–99.7% (N=627); RhD typing, 98.6%–99.8% (N=607); crossmatching, 90.1%–98.1% (N=454); ABO subtyping, 92.7%–93.0% (N=42); Rh CcEe antigen testing, 97.8%–100.0% (N=46); weak D test, 70.8%–73.6% (N=91); antibody screening, 96.6%–100.0% (N=267); direct antiglobulin test (DAT) using a polyspecific reagent, 92.2%–99.6% (N=49); DAT using an immunoglobulin G monospecific reagent, 84.1%–100.0% (N=68); DAT using a C3d monospecific reagent, 80.0%–100.0% (N=68); antibody identification, 96.7%–99.1% (N=118); and ABO antibody titration, 82.4%–100.0% (N=42). Excellent results for the 2016 EQA for immunohematology testing were obtained, except for those of the weak D test. The EQA for immunohematology testing in 2016 should be helpful for improving the quality of participating laboratories.