摘要
En Guatemala, los huevos de tortuga parlama son aprovechados para su comercialización. El esquema de conservación se basa en un sistema de cuotas, en la que se establece que el 20% de los huevos recolectados deberán ser entregados a un tortugario registrado. Se estima que alrededor de 673,304 huevos son puestos por año en la Costa Pacífica con un valor en playa aproximado de Q1,124,869.00. En Guatemala, el aprovechamiento de huevos de tortuga funciona como un amortiguador clave en las economías de las comunidades. En los últimos años, la compra de huevos por parte de los tortugarios ha aumentado. Estos criaderos, además de recibir la cuota de conservación, compran el resto de la carga de huevos del nido. Como resultado, el número de nidos plantados en los tortugarios también ha aumentado. Sin embargo, esto también ha provocado que haya un mercado mas grande para los huevos de tortuga. La venta de huevos se ha convertido en una fuente de ingresos muy exitosa con menos riesgo y costos que la pesca. Esto ha llevado a algunos pescadores artesanales a cambiar a la recolección de huevos como su actividad principal. Es necesario evaluar los esquemas de conservación actuales y la efectividad de los incentivos de manera integral, tomando en cuenta impactos ambientales, rentabilidad y efectos sociales. El presente análisis aporta información útil para identificar la calidad de los incentivos, para poder reformar o eliminar los incentivos ineficientes o perversos, de manera que los escasos recursos de conservación se puedan invertir de la manera más efectiva
In Guatemala, olive ridley sea turtle (Lepidochelys olivacea) eggs are harvested. The conservation scheme is based Modelo de publicación sin fines de lucro para conservar la naturaleza académica y abierta de la comunicación científica on a quota system, which requires that 20% of the collected eggs be delivered to a registered turtle nursery. It is estimated that approximately 673,304 eggs are laid annually on the Pacific coast, with an approximate beach value of Q1,124,869.00. In Guatemala, the harvesting of turtle eggs serves as a key buffer in the economies of coastal communities. In recent years, the purchase of eggs by turtle nurseries has increased. These nurseries, in addition to receiving the conservation fee, buy the rest of the load of eggs from the nest. As a result, the number of planted nests in nurseries has also increased. However, this has also led to a larger market for turtle eggs. The sale of eggs. The sale of eggs has become a highly successful source of income, with fewer risks and costs than fishing. This has led some artisanal fishermen to switch to egg collection as their main activity. This analysis provides useful infromation for identifying the quality of incentives, in order to reform or elinate inefficient or perverse incentives, so that scarce conservation resource will allow for the identification of the quality of the incentives, aiming to reform or eliminate inefficient or perverse incentives so that scarce conservation resources can be invested in the most effective way
摘要
A rapid analytical method for simultaneous determination of 32 kinds of multi-residue veterinary drugs in eggs was developed using a modified QuEChERS technique based on a reduced graphene oxide-coated melamine sponge(r-GO@MeS)by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The influences of graphene oxide(GO)concentrations,sponge dosages,and purification modes on drug recoveries were investigated during the purification process.The optimal purification conditions involved using a GO concentration of 0.5 mg/mL,a sponge dosage of 6.0 cm3/mL,and a dynamic purification mode of 5 extrusion cycles.Separation was achieved using an Agilent Eclipse Plus C18 RRHD column(100 mm×2.1 mm,1.8 μm),and quantitative analysis was performed by the external standard method using an electrospray ionization source(ESI)in multiple reaction monitoring(MRM)mode.The results showed that all 32 kinds of veterinary drugs exhibited good linear correlation with coefficients greater than 0.999,and matrix effects(MEs)ranging from?7.8%to 18.9%.The limits of detection(LODs)and quantification(LOQs)ranged from 0.2 to 10.2 μg/kg and from 0.6 to 28.0 μg/kg,respectively.The recoveries for the three spiked levels were in the range of 66.5%?117.5%,with intra-day and inter-day precision(Relative standard deviation)below 13.3%and 16.3%,respectively.The synthetic r-GO@MeS exhibited efficient matrix purification without the need of high-speed centrifugation or strong magnetic field assistance.This significantly shorted the sample pretreatment time and improved the convenience of the matrix purification process.Combined with UPLC-MS/MS,the method was suitable for the rapid determination of multi-residue veterinary drugs in eggs.
摘要
Objective To investigate the distribution characteristics of serum sIgE antibodies against four allergenic protein components of egg white in children with egg allergy,and then clarify the clinical application value of single component-resolved diagnostics of egg allergy.Methods Serum samples from 197 children with egg allergy were collected.The levels of serum sIgE antibodies against four major allergenic protein components of egg white,including ovomucin,ovalbumin,ovotransferrin,and lysozyme,were detected by the light-excited chemiluminescence assay(LiCA),and the distribution characteristics of sIgE antibodies were analyzed.Results The positive rates of serum sIgE antibodies against ovalbumin,ovomucin,ovotransferrin,and lysozyme in 197 chlidren with egg allergy were 77.16%(152/197),70.56%(139/197),35.02%(69/197),and 18.27%(36/197),respectively.The positive rate of serum sIgE antibody against both ovomucin and ovalbumin was 30.45%.There was a weak correlation between the levels of sIgE antibodies against egg and the cumulative levels of sIgE antibodies against four allergenic protein components(r=0.266 8,P＜0.05).There were signifi-cant individual differences in the levels of serum sIgE antibodies against four allergenic protein components of egg white in the children with egg allergy.Conclusion There is individual heterogeneity in the levels of serum sIgE antibodies against four components of egg white in the children with egg allergy.The detection of sIgE antibodies against egg white components can distinguish different forms of egg allergies,which is of great value for the accurate diagnosis and precise desensitization of children's egg allergy.
摘要
【Objective】 To comprehensively explore the serological characteristics of anti-PP1Pk and potential therapeutic strategies for recurrent miscarriage in p-blood type pregnant women. 【Methods】 Neutralization with pigeon egg white and human plasma, disruption of IgM antibodies by 2-mercaptoethanol reagent, and complement adding were conducted. Anti-PP1Pk titers under different processing conditions, media and temperatures were determined, and neutralizing effect of human plasma on anti-PP1Pk and its sensitization complement ability were analyzed. 【Results】 The titers of anti-PP1Pk in saline and column agglutination were 4 and 8, respectively. Low temperature increased titers, while β-mercaptoethanol treatment significantly reduced them. Pigeon egg white partially neutralized anti-PP1Pk antibodies. Human plasma was also capable of reducing anti-PP1Pk titers with neutralization capability surpassing that of pigeon egg white, and there were individual differences in neutralization capability. 【Conclusion】 The anti-PP1Pk was a blend of antibodies encompassing both IgM and IgG types, exhibiting cold reactivity, and having the potential for complement activation. Human plasma emerges as an effective modulator for diminishing the efficacy of anti-PP1Pk. Plasma transfusion holds promise as a therapeutic avenue for addressing recurrent miscarriages in pregnant individuals with the p phenotype.
摘要
The use of alternative nutritional sources is an important topic for animal production such as poultry. This study examined the effect of replacing soybean meal with sunflower leaf meal in the diet of laying shaver brown pullets. A total of 30 laying birds were assigned to three treatments of 0% (as control group), 10% and 15% MSFLM inclusion. Four eggs per replicate were randomly taken for three consecutive days at two weeks interval for five times, and 18 weeks individuals were selected after 40 days of experimental time. External parameters of the eggs (egg weight, egg length, egg breadth and egg shape index, shell thickness), and body parameters (Final body weight, weight gain), feed intake and hen day production were measured. For egg length, egg breadth and shell thickness showed significant difference (p< 0.05) in from the control birds. Final body weight, weight gain, feed intake and hen day production showed comparable results with values from eggs of birds on control diet. The study revealed the ability of birds to easily utilize the nutrients in the protein sources. MSFLM utilization up to 15% in pullets' diet revealed no detrimental effect on the performance of the laying birds and the external qualities of eggs produced.
O uso de fontes nutricionais alternativas é um tópico importante para a produção animal, como a avicultura. Este estudo examinou o efeito da substituição do farelo de soja por farelo de folha de girassol na dieta de frangas marrons barbeadoras poedeiras. Um total de 30 aves poedeiras foram designadas a três tratamentos de 0% (como grupo controle), 10% e 15% de inclusão MSFLM. Quatro ovos por repetição foram retirados aleatoriamente por três dias consecutivos em intervalos de duas semanas por cinco vezes, e indivíduos de 18 semanas foram selecionados após 40 dias do tempo experimental. Parâmetros externos dos ovos (peso do ovo, comprimento do ovo, largura do ovo e índice de forma do ovo, espessura da casca) e parâmetros corporais (peso corporal final, ganho de peso), consumo de ração e produção diária da galinha foram medidos. Para comprimento do ovo, largura do ovo e espessura da casca apresentaram diferença significativa (p <0,05) nas aves controle. Peso corporal final, ganho de peso, consumo de ração e produção de dia de galinha mostraram resultados comparáveis ââcom valores de ovos de aves em dieta controle. O estudo revelou a capacidade das aves de utilizar facilmente os nutrientes das fontes de proteína. A utilização de MSFLM até 15% na dieta das frangas não revelou nenhum efeito prejudicial no desempenho das aves poedeiras e nas qualidades externas dos ovos produzidos.
Subject(s)
Animals , Poultry/growth & development , Chickens/growth & development , Diet , Helianthus摘要
Abstract The use of alternative nutritional sources is an important topic for animal production such as poultry. This study examined the effect of replacing soybean meal with sunflower leaf meal in the diet of laying shaver brown pullets. A total of 30 laying birds were assigned to three treatments of 0% (as control group), 10% and 15% MSFLM inclusion. Four eggs per replicate were randomly taken for three consecutive days at two weeks interval for five times, and 18 weeks individuals were selected after 40 days of experimental time. External parameters of the eggs (egg weight, egg length, egg breadth and egg shape index, shell thickness), and body parameters (Final body weight, weight gain), feed intake and hen day production were measured. For egg length, egg breadth and shell thickness showed significant difference (p 0.05) in from the control birds. Final body weight, weight gain, feed intake and hen day production showed comparable results with values from eggs of birds on control diet. The study revealed the ability of birds to easily utilize the nutrients in the protein sources. MSFLM utilization up to 15% in pullets diet revealed no detrimental effect on the performance of the laying birds and the external qualities of eggs produced.
Resumo O uso de fontes nutricionais alternativas é um tópico importante para a produção animal, como a avicultura. Este estudo examinou o efeito da substituição do farelo de soja por farelo de folha de girassol na dieta de frangas marrons barbeadoras poedeiras. Um total de 30 aves poedeiras foram designadas a três tratamentos de 0% (como grupo controle), 10% e 15% de inclusão MSFLM. Quatro ovos por repetição foram retirados aleatoriamente por três dias consecutivos em intervalos de duas semanas por cinco vezes, e indivíduos de 18 semanas foram selecionados após 40 dias do tempo experimental. Parâmetros externos dos ovos (peso do ovo, comprimento do ovo, largura do ovo e índice de forma do ovo, espessura da casca) e parâmetros corporais (peso corporal final, ganho de peso), consumo de ração e produção diária da galinha foram medidos. Para comprimento do ovo, largura do ovo e espessura da casca apresentaram diferença significativa (p 0,05) nas aves controle. Peso corporal final, ganho de peso, consumo de ração e produção de dia de galinha mostraram resultados comparáveis com valores de ovos de aves em dieta controle. O estudo revelou a capacidade das aves de utilizar facilmente os nutrientes das fontes de proteína. A utilização de MSFLM até 15% na dieta das frangas não revelou nenhum efeito prejudicial no desempenho das aves poedeiras e nas qualidades externas dos ovos produzidos.
摘要
Women undergoing in vitro fertilization-embryo transfer (IVF-ET) may experience repeated implantation failure (RIF). Under the guidance of "Yu Pei Qi Sun" theory, Traditional Chinese Medicine (TCM) compounds can improve the quality of sperm and eggs of both husband and wife by anti-oxidative stress, improving mitochondrial function and reducing excessive apoptosis before IVF-ET to obtain high-quality embryos; TCM compounds can also promote angiogenesis, anti-oxidative stress, regulation of estrogen and progesterone receptor content, regulate immunity to improve female endometrial receptivity to facilitate embryo implantation; it can also invigorate the spleen and kidney after transplantation and caring for the fetus to help implantation, as well as alleviate the anxiety of patients during transplantation.
摘要
Objective To know the residues of 13 veterinary drug residues in chicken and eggs foods in some areas in Xinjiang. Methods A total of 170 chicken and egg samples were randomly selected from supermarkets and farmers' markets in seven cities in Xinjiang. Eleven quinolone antibiotics, two tetracycline antibiotics, ribavirin and metronidazole were examined for veterinary drug residues using liquid chromatography tandem mass spectrometry (LC-MS/MS) . Results The overall detection rate of veterinary drug residues in eggs and chicken were 20%（18/90）and11.25%（9/80）. The overall over-standard rate were 18.89%（17/90）and 0（0/80）. Veterinary drug residues in chicken are heavier than eggs. Veterinary drugs were detected and over-standarded in all seven cities in Xinjiang monitored. Conclusion The veterinary drug contamination in chicken and eggs in Xinjiang is relatively serious. It is recommended to strengthen the standardization of production and supervision to ensure food safety.
摘要
Since the occurrence of the first case of "egg freezing for single women" in China, it has aroused different discussions and thoughts from all walks of life. Through the ethical analysis of the four principles of bioethics, this paper found a reasonable ethical basis for single women’s egg freezing. From the perspective of favorable principle, egg freezing could temporarily preserve women’s fertility, which is conducive to women’s better realization of their own personal value and social values. From the perspective of no harm principle, no harm is relative, and the harm brought by egg freezing technology is within the acceptable and permissible range. From the perspective of justice principle, whether there is gender discriminatory in that men could freeze their sperm but women have many restrictions on egg freezing. From the principle of respect for autonomy, whether to choose egg freezing is a manifestation of women’s autonomy rights, which should be respected. This paper tried to propose corresponding solutions to the ethical and legal issues caused by the permission of egg freezing from the legal perspective, the medical ethics committee’s responsibilities, and the ethical level.
摘要
@#Abstract: Objective To construct a mouse model of Schistosoma japonicum liver disease induced by direct injection of Schistosoma japonicum eggs through the portal vein and evaluate its effectiveness, in order to provide a new animal model for schistosomiasis liver disease research. Methods Fifteen 8-week-old C57BL/6 male mice were randomly divided into control group and egg injection group, with 5 in the control group and 10 in the egg injection group. On day -14, 5 000 live eggs were injected into the abdominal cavity of mice, and on day 0, the mice were anesthetized and the abdominal cavity was opened. 5 000 live eggs were injected through the portal vein, and the control group was injected with equal volume of phosphate buffer (PBS). 5 mice in the egg group were killed on day 10 and 30, respectively. The control group mice were killed on day 10, and their serum and liver tissue were collected. Hematoxylin eosin staining (HE) and Masson staining were performed, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), and liver hydroxyproline (HYP) content were detected using a microplate spectrophotometer. Liver fibrosis-related genes, Th1 and Th2 type immune response-related genes were analyzed by real-time fluorescence quantitative PCR (qPCR). Liver injury, egg granuloma and fibrosis, and adaptive immune response were detected to evaluate the effect of portal vein injection of eggs while inducing mouse model of schistosomiasis liver disease. Results The results showed that significant egg granulomas appeared in the liver of mice after injection of eggs into the portal vein for 10 and 30 days. There was no statistically significant difference in the area of egg granulomas between the 10-day group and the 30-day group (t=0.975, P=0.332). Masson staining and liver hydroxyproline content detection showed significant fibrosis in the liver. The qPCR results showed that, compared with the control group, the expression levels of fibrosis marker genes, such as α⁃Sma (alpha smooth muscle actin), Col1a1 (collagen type Ⅰ alpha 1), and Tgfb1 (transforming growth factor beta 1), were significantly increased (t=6.380, 7.533, 5.314; P=0.002, 0.001, 0.007), and then decreased on the 30th day, with no statistical difference compared to the control group (t=0.940, 1.529, 1.746; P=0.778, 0.543, 0.457). At the same time, the expression levels of Th1 type immune response-related genes, such as tumor necrosis factor alpha (Tnf), interferon gamma (Ifng), and Th2 type immune response-related genes, such as interleukin-5 (Il5), interleukin-13 (Il13), significantly increased 10 days after eggs injection (t=6.163, 4.589, 5.651, 5.367; P=0.003, 0.018, 0.020, 0.009). In addition, there was no significant change in the levels of AST and ALT in the serum of each group of mice (t=0.982, 3.450; P=0.771, 0.074. t=1.164, 0.564; P=0.697, 0.917). Conclusions A mouse model of schistosomiasis liver disease induced by portal vein injection of worm eggs was constructed. The study provides a new modeling method for studying the mechanism of liver fibrosis in schistosomiasis..
摘要
Gastrointestinal parasites are a significant challenge in sheep farming, and periparturient ewes are one of the most susceptible categories in sheep flocks. This study aimed to verify whether the injection of B vitamins at the acupuncture point governing vessel 14 (GV14) can influence the parasitic load or host-parasite interaction in naturally infected ewes during the peripartum period. Four weeks before parturition, 25 ewes were divided into five treatment groups: (1) Dose Control: 0.2 mL of B complex via IM; (2) Drug Control: 2 mL Complex B via IM; (3) Acupoint Control: 0.2 mL of Water at GV14 point; (4) Control; (5) Test group: 0.2 mL of B complex at the GV14 point. Treatments were performed at weeks -3, -1, 2, 4, and 6 about the parturition time. Fecal samples to estimate the parasitic load by fecal egg counts (FEC) and total blood samples for hemogram examination were performed seven days after the treatment, coinciding with weeks -2, 3, 5, and 7 about the parturition time. The number of peripheral eosinophils was higher in group 3 than in group 1 (p <0.05). Although groups 3 and 5 had lower mean values of FEC and higher hematological values at the time of weaning, these differences were not statistically significant (p> 0.05) according to the F test. However, all animals in groups 3 and 5 maintained low parasitism levels until the end of the experiment. Further studies should be performed with larger sample sizes and minor changes to the experimental protocol to elucidate the role of GV14 acupoint stimulation in the host-parasite relationship.(AU)
A parasitose gastrointestinal é o principal desafio sanitário para a criação de ovinos a pasto, e as ovelhas no periparto são uma das categorias mais susceptíveis a esta infeção no rebanho. O objetivo deste trabalho foi verificar se a injeção de vitaminas do complexo B no acuponto Vaso Governador 14 (VG14) poderia influenciar a carga parasitária ou modular a interação hospedeiro-parasito em ovelhas naturalmente infectadas no período de periparto. Quatro semanas antes da data prevista para o parto, 25 ovelhas foram divididas em cinco grupos de tratamento (1) Controle da dose: 0,2 mL de vitaminas do complexo B via IM; (2) Controle do fármaco: 2mL complexo B via IM; (3) Controle do Ponto: 0,2 mL de água destilada no ponto VG14; (4) Controle sem tratamento; (5) Grupo teste: 0,2 mL de complexo B no pontoVG14. Estes tratamentos foram realizados nas semanas -3; -1; 2; 4 e 6 em relação à data do parto. Amostras de fezes para contagem de ovos por grama de fezes (OPG), assim como amostras de sangue para realizar o hemograma, foram obtidas dos animais sete dias após os tratamentos, coincidindo com as semanas -2, 3, 5 e 7 do parto. O número de eosinófilos periféricos foi superior no grupo 3 se comparado ao grupo 1 (p <0,05). Os animais dos grupos 3 e 5 tiveram menores valores de OPG e maior hematócrito, mas estas diferenças não foram consideradas significativas pelo teste F (p> 0,05). No entanto, todas as ovelhas dos grupos 3 e 5 se mantiveram com níveis baixos de parasitismo durante todo o experimento. Sugere-se que novos estudos sejam realizados com algumas modificações deste protocolo a fim de aprofundar o conhecimento do potencial imunomodulador do acuponto VG14.(AU)
Subject(s)
Animals , Female , Pregnancy , Sheep/parasitology , Acupuncture Therapy/veterinary , Communicable Disease Control/methods , Vitamin B Complex/analysis , Peripartum Period , Gastrointestinal Diseases摘要
To compare the viability of periodontal ligament (PDL) cells stored in Hanks Balanced Salt Solution (HBSS) with those in readily available transport media over a variable period of time. Methods: Periodontal ligament cells harvested from premolars freshly extracted for orthodontic reasons were cultured for exponential growth. The cells were exposed to egg white, evaporated milk, water and Hanks Balanced Salt Solution (HBSS) at room temperature. Their viability was evaluated after 30 minutes, 1 hour and 3 hours with the tetrazolium salt-based colorimetric assay (MTT assay). Statistical analysis was done using the IBM® SPSS version 23.0 software. Comparison between the Mean Optical Densities (MODs) of the cells stored in HBSS and other media at each time interval was done using the independent t test. Repeated measure ANOVA and Tukey post-hoc test were also carried out to compare the MOD of cells within each medium over time. The level of significance was set at p <0.05. Result: The PDL cells stored in egg white had higher MODs than those in HBSS at 30 minutes and 1 hour. Conversely, the MODs of the cells stored in milk and water were lower than those in HBSS at all the studied points. There was a significant difference in the viability of the cells stored in HBSS and water at all the time points (p<0.05). Conclusion: For up to an hour, egg white was found to perform better than HBSS in supporting the viability of PDL cell
Subject(s)
Periodontal Ligament , Tooth Avulsion , Milk , Egg White , Saline Solution摘要
The life cycle of stink bug, Glyphepomis dubia and the development of two egg parasitoids (Telenomus podisi and Trissolcus basalis) were studied at the Federal University of Maranhão, at 26 ± 2oC, relative humidity (RH) of 60 ± 10% and 12h photophase. Individuals used in the study were collected from seven rice fields located around the municipality of Arari, Maranhão, Brazil, and maintained in greenhouse and laboratory for the life cycle studies. From egg to adult, G. dubia took 35.2 days to complete the life cycle. The oviposition period was 37 days, with egg masses of about 12 eggs each and viability of 93.1%. Longevity was 53 and 65 days for females and males, respectively. The egg parasitoids Te. podisi and Tr. basalis parasitized and developed in G. dubia eggs; however, the biological characteristics of Tr. basalis were affected. Emergence of the parasitoids was higher for Te. podisi (83.5%) compared to the records for Tr. basalis (50.4%). Therefore, G. dubia may potentially achieve a pest status and Te. podisi is a promising biological control agent for G. dubia management in Brazil due to its higher longevity and better reproductive parameters.
O ciclo de vida do percevejo, Glyphepomis dubia e a biologia de dois parasitoides de ovos (Telenomus podisi e Trissolcus basalis) foram estudados na Universidade Federal do Maranhão, a 26 ± 2oC, umidade relativa (UR) de 60 ± 10% e fotofase de 12h. Sete indivíduos de G. dubia foram coletados em lavoura de arroz localizada no município de Arari, Maranhão, Brasil e mantidos em casa de vegetação e laboratório para estudos de ciclo de vida. Do ovo ao adulto, G. dubia levou 35.2 dias para completar o ciclo de vida. O período de oviposição foi de 37 dias com massas de ovos com cerca de 12 ovos/massa e viabilidade de 93.1%. A longevidade foi de 53 e 65 dias, respectivamente, para fêmeas e machos. Os parasitoides de ovos, Te. podisi e Tr. basalis parasitaram e se desenvolveram em ovos de G. dubia, no entanto as características biológicas de Tr. basalis foi afetada. A emergência dos parasitoides foi maior para Te. podisi (83.5%) em comparação com o registrado para Tr. basalis (50.4%). Portanto, G. dubia poderá apresentar potencial para atingir o status de praga e Te. podisi é um promissor agente de controle biológico para ser utilizado no manejo de G. dubia no Brasil, pois apresentou maior longevidade e os melhores parâmetros reprodutivos.
Subject(s)
Animals , Cimicidae/growth & development , Cimicidae/parasitology , Pest Control, Biological/methods , Hymenoptera/growth & development , Oryza/parasitology摘要
Abstract The life cycle of stink bug, Glyphepomis dubia and the development of two egg parasitoids (Telenomus podisi and Trissolcus basalis) were studied at the Federal University of Maranhão, at 26 ± 2oC, relative humidity (RH) of 60 ± 10% and 12h photophase. Individuals used in the study were collected from seven rice fields located around the municipality of Arari, Maranhão, Brazil, and maintained in greenhouse and laboratory for the life cycle studies. From egg to adult, G. dubia took 35.2 days to complete the life cycle. The oviposition period was 37 days, with egg masses of about 12 eggs each and viability of 93.1%. Longevity was 53 and 65 days for females and males, respectively. The egg parasitoids Te. podisi and Tr. basalis parasitized and developed in G. dubia eggs; however, the biological characteristics of Tr. basalis were affected. Emergence of the parasitoids was higher for Te. podisi (83.5%) compared to the records for Tr. basalis (50.4%). Therefore, G. dubia may potentially achieve a pest status and Te. podisi is a promising biological control agent for G. dubia management in Brazil due to its higher longevity and better reproductive parameters.
Resumo O ciclo de vida do percevejo, Glyphepomis dubia e a biologia de dois parasitoides de ovos (Telenomus podisi e Trissolcus basalis) foram estudados na Universidade Federal do Maranhão, a 26 ± 2oC, umidade relativa (UR) de 60 ± 10% e fotofase de 12h. Sete indivíduos de G. dubia foram coletados em lavoura de arroz localizada no município de Arari, Maranhão, Brasil e mantidos em casa de vegetação e laboratório para estudos de ciclo de vida. Do ovo ao adulto, G. dubia levou 35.2 dias para completar o ciclo de vida. O período de oviposição foi de 37 dias com massas de ovos com cerca de 12 ovos/massa e viabilidade de 93.1%. A longevidade foi de 53 e 65 dias, respectivamente, para fêmeas e machos. Os parasitoides de ovos, Te. podisi e Tr. basalis parasitaram e se desenvolveram em ovos de G. dubia, no entanto as características biológicas de Tr. basalis foi afetada. A emergência dos parasitoides foi maior para Te. podisi (83.5%) em comparação com o registrado para Tr. basalis (50.4%). Portanto, G. dubia poderá apresentar potencial para atingir o status de praga e Te. podisi é um promissor agente de controle biológico para ser utilizado no manejo de G. dubia no Brasil, pois apresentou maior longevidade e os melhores parâmetros reprodutivos.
摘要
Abstract The life cycle of stink bug, Glyphepomis dubia and the development of two egg parasitoids (Telenomus podisi and Trissolcus basalis) were studied at the Federal University of Maranhão, at 26 ± 2oC, relative humidity (RH) of 60 ± 10% and 12h photophase. Individuals used in the study were collected from seven rice fields located around the municipality of Arari, Maranhão, Brazil, and maintained in greenhouse and laboratory for the life cycle studies. From egg to adult, G. dubia took 35.2 days to complete the life cycle. The oviposition period was 37 days, with egg masses of about 12 eggs each and viability of 93.1%. Longevity was 53 and 65 days for females and males, respectively. The egg parasitoids Te. podisi and Tr. basalis parasitized and developed in G. dubia eggs; however, the biological characteristics of Tr. basalis were affected. Emergence of the parasitoids was higher for Te. podisi (83.5%) compared to the records for Tr. basalis (50.4%). Therefore, G. dubia may potentially achieve a pest status and Te. podisi is a promising biological control agent for G. dubia management in Brazil due to its higher longevity and better reproductive parameters.
Resumo O ciclo de vida do percevejo, Glyphepomis dubia e a biologia de dois parasitoides de ovos (Telenomus podisi e Trissolcus basalis) foram estudados na Universidade Federal do Maranhão, a 26 ± 2oC, umidade relativa (UR) de 60 ± 10% e fotofase de 12h. Sete indivíduos de G. dubia foram coletados em lavoura de arroz localizada no município de Arari, Maranhão, Brasil e mantidos em casa de vegetação e laboratório para estudos de ciclo de vida. Do ovo ao adulto, G. dubia levou 35.2 dias para completar o ciclo de vida. O período de oviposição foi de 37 dias com massas de ovos com cerca de 12 ovos/massa e viabilidade de 93.1%. A longevidade foi de 53 e 65 dias, respectivamente, para fêmeas e machos. Os parasitoides de ovos, Te. podisi e Tr. basalis parasitaram e se desenvolveram em ovos de G. dubia, no entanto as características biológicas de Tr. basalis foi afetada. A emergência dos parasitoides foi maior para Te. podisi (83.5%) em comparação com o registrado para Tr. basalis (50.4%). Portanto, G. dubia poderá apresentar potencial para atingir o status de praga e Te. podisi é um promissor agente de controle biológico para ser utilizado no manejo de G. dubia no Brasil, pois apresentou maior longevidade e os melhores parâmetros reprodutivos.
Subject(s)
Humans , Animals , Oryza , Wasps , Heteroptera , Hemiptera , Hymenoptera , Oviposition , Ovum , Biology摘要
Introdução: A vacina contra a febre amarela é cultivada em ovos embrionados de galinha e por isso pode estar contraindicada em indivíduos alérgicos ao ovo. Quando indicada, deve ser aplicada com cautela, após atendimento especializado para avaliação de testes e necessidade de dessensibilização. Sua segurança nos alérgicos ao ovo ainda é pouco estudada. Objetivo: Descrever uma população pediátrica encaminhada por alergia ao ovo, com ou sem diagnóstico comprovado, e os casos de eventos adversos do tipo imediata à vacina contra a febre amarela em um centro de referência para imunobiológicos especiais (CRIE). Material e métodos: Estudo transversal realizado com coleta de dados retrospectivos de crianças entre 9 meses e 12 anos de idade, vacinadas contra a febre amarela com história de alergia ao ovo, no período de 2018 a 2019. Resultados: Dentre as 829 crianças, com diagnóstico presumido de alergia ao ovo, foi identificada uma maior prevalência de sintomáticos após exposição ao ovo, com IgE específica detectável para ovo, clara de ovo e/ou ovoalbumina. Testes para vacina febre amarela foram realizados em 25 crianças com suspeita de alergia grave ou anafilaxia ao ovo, sendo 15 (60%) positivos com a vacina aplicada após dessensibilização. Foram evidenciados apenas 11 (1,3%) casos de evento adverso imediato à vacina, todos classificados como evento adverso não grave e com acometimento especial da pele (reação local e exantema ou urticária). A maioria dos eventos ocorreu em menores de 2 anos, nos sintomáticos após ingesta de ovo e naqueles com altos valores de IgE específica para clara de ovo. Conclusão: Este estudo evidencia que a vacina contra a febre amarela pode ser aplicada em crianças alérgicas ao ovo, de forma segura, inclusive naquelas com história de anafilaxia, desde que em ambiente adequado e com profissionais especializados.
Introduction: The yellow fever vaccine is grown in embryonated chicken eggs and may be contraindicated for egg-allergic individuals. When indicated, it should be applied with caution, after testing and desensitization. Its safety in egg-allergic patients is still poorly studied. Objective: To describe a pediatric population referred for egg allergy, with or without a confirmed diagnosis, and cases of immediate-type adverse events to the yellow fever vaccine at a reference center for special immunobiologicals. Material and methods: This cross-sectional study collected retrospective data from children between 9 months and 12 years of age who were vaccinated for yellow fever between 2018 and 2019 and had a history of egg allergy. Results: In the 829 children diagnosed with presumed egg allergy, a higher prevalence of symptoms was identified after egg exposure, with detectable specific IgE for egg, egg white, and/ or egg albumin. Yellow fever vaccine tests were performed in 25 children suspected of severe allergy or anaphylaxis to eggs, and 15 (60%) tested positive to the vaccine after desensitization. Only 11 (1.3%) cases of immediate adverse events to the vaccine occurred, all classified as non-serious events that especially involved the skin (local reaction and rash or urticaria). Most events occurred in children under 2 years of age, those symptomatic after egg ingestion, and those with high levels of specific IgE to egg white. Conclusion: This study demonstrated that the yellow fever vaccine can be safely administered to egg-allergic children, including those with a history of anaphylaxis, in an appropriate environment and with specialized professionals.
Subject(s)
Humans , Infant , Child, Preschool , Child摘要
This study aimed to assess the effect of natural antioxidants on storage time, physical and chemical properties of egg. A total of 200 laying ISA brown birds were distributed into eight dietary groups, each dietary group with 25 birds (5 birds per replicate). They were fed with roselle, black pepper, green tea, combine (roselle + black pepper + green tea) at 0.5 g/kg and 1.0 g/kg in basal diet respectively and a control feed. At the end of eight (8) weeks of feeding trial, twelve eggs were collected from each dietary group (six eggs per dietary group were analyzed for the internal and external properties while the remaining six were stored). Data collected include; Egg shape index, egg weight, shell thickness, membrane weight, yolk index, haugh unit, meat and blood spot, yolk colour, lipid profile, lipid peroxidation and proximate analysis. Data generated were subjected to Analysis of variance using the General Linear Model for factorial within a completely randomized design. The natural antioxidants significantly (P<0.05) improves the proximate composition of the poultry eggs. Both Green tea and Black pepper have significant effect (P<0.05) on the yolk percentage. Black pepper increases (P<0.05) the Haugh unit while it shows to be lower (P>0.05) in the combination of the antioxidants. The inclusion levels of the natural antioxidants on the internal and external quality of egg reveals that there were no significant (P>0.05) differences in the two inclusion level of 0.5 g/kg and 1.0 g/kg of feed but they have numerically higher values in the external parameters and internal parameters. Eggs stored for 4 weeks had the lowest value (P>0.05) for the proximate and lipid profiles, though there are no significant (P>0.05) differences in their values. The fresh eggs show high moisture content (P<0.05) but the value for nitrogen free extract is low (P>0.05) in the fresh eggs. The natural antioxidants improved significantly (P<0.05) the proximate composition of the poultry eggs, with the green tea having the highest value (P<0.05) for Moisture contents and CP, black pepper the highest (P<0.05) for CP Based on the result obtained from this study, the natural antioxidants (black pepper, green tea and roselle) in layers� diet shows significant effects on the physical qualities of egg and the yolk color was also preferred with the inclusion of the natural antioxidants compared to the control. The chemical properties also deteriorate with the storage time. Natural antioxidants are hereby recommended for better and improved chemical qualities of eggs.
摘要
El presente escrito aborda la cuestión del proyecto monomarental, sus derroteros y periplos singulares. Al tiempo que en ellos se vislumbra un camino muchas veces compartido y repetido. La temática reviste una presencia estadística en aumento a nivel mundial que visibiliza cambios sociales, económicos y de derechos en la agenda femenina. El objetivo central del escrito radica en describir en primera persona las circunstancias que llevaron a Helen a recurrir a la ovodonación como método reproductivo y los duelos que dicho recorrido encierra. En síntesis, en la historia de Helen se vislumbra de modo paradigmático el encuentro con la imposibilidad del propio cuerpo reproductivo y la solución que ofrece la medicina; al tiempo que en otra cuerda, se trasluce la dimensión singular y clínica de las decisiones subjetivas por las que ella deberá responder
This paper addresses the question of the singleparent project, its paths and unique journeys. At the same time that in them a path many times shared and repeated is glimpsed. The issue has a growing statistical presence worldwide that makes visible social, economic and rights changes on the women's agenda. The central objective of the writing lies in describing in the first person the circumstances that led Helen to resort to egg donation as a reproductive method and the duels that this journey entails. In short, in Helen's story, the encounter with the impossibility of the reproductive body itself and the solution offered by medicine is glimpsed in a paradigmatic way; while on another string, the singular and clinical dimension of the subjective decisions for which she will have to answer shines through
Subject(s)
Humans , Female , Pregnancy , Child , Adolescent , Adult , Oocyte Donation , Biological Clocks , Birth Rate , Reproductive Health , Infertility, Female , Mothers摘要
The purpose of this trial was to assess the influence of homeopathy products on the diet of quails based on egg quality when submitted to different storage periods. Methodology: Two hundred, 45 day old Japanese quails and 80% of production were used, in a completely randomized design comprised of a 4x3 factorial, and 4 diets (basal feed, inert vehicle and 2 homeopathic products: Fertsigo® (Sulphur10 CH, Sepia 15CH ) and Ovosigo® (Belladonna 12CH,Silicea 12 CH, Natrum mur. 30CH, Calcarea phos. 30CH, Sulphur 12CH) and 3 storage periods (0 days, 7 days and 14 days) with ten repetitions of three eggs per treatment. The weight, percentages of yoke, albumen and shell, albumen height and yolk color, specific gravity, Haugh unit, yolk index and shell thickness were evaluated. The data were submitted to variance analysis to verify whether there was a interaction effect between homeopathy factors and storage time, and when absent, the isolated effects. Results: An interaction between the homeopathic products and time was found for the parameters of albumen height and yolk, Haugh unit and yolk index, which reduced over time. For egg weight, yolk, albumen and shell, a significant effect (p<0.05) was found only in the case of homeopathy for the percentages of albumen and shell. For egg and albumen weights, yolk and albumen percentages, specific gravity and colorimetry there was an effect for time, however these parameters reduced over the storage time in days. The addition of the homeopathic based products Ovosigo® and FertSigo® are indicated for the diets of Japanese quail during the laying phase since it resulted in better weights for the egg and its components. Conclusion: The different homeopathic products did not have an influence on conserving the quality of the Japanese quail eggs during the periods evaluated.
Subject(s)
Quail , Homeopathic Remedy , Eggs , Food, Organic摘要
Ayurveda Samithas directly or indirectly touch upon each dimension of human life. The leads of every science connected to health can be traced in Ayurveda. Ahara Dravyas explained by Acharays plays an important role in prevention and curation of diseases. Meat of various animals and birds are mentioned with their nutritive and therapeutic values under the sub class of Mamsa varga. Kukkuta is one such bird belonging under Vikshira varga. Among many birds, the instances of Kukkuta (Chicken) outplay the importance of others birds. Chickens are the important source of meat and egg which greatly benefits human population with its high nutritive profile. Kukkuta and its eggs are not only used for its food values but also recommended for therapeutic values in various diseases, formulations and in as Pathya-apathya in Ayurveda. As the information on Kukkuta is scattered, the present article compiles, analyses and discuss the utility of Kukkuta in detail