摘要
Objective To explore the regulatory mechanism of microRNA-224 (miR-224) in the apoptosis and proliferation of human cholangiocarcinoma (CC) cells and examine whether the pathway of miR-224-HOXD10-PAK4 exist in cc cells or not.Methods The expression of miR-224 in CC and adjacent normal tissue were measured by RT-PCR.QBC939 cells were chosen and divided into two groups:cell groups transducted with negative control viruses (NC group) and cell groups transducted with target gene miRNA-down virus (DOMN group).The rate of apoptosis and the number of colonies formed in QBC939 cell lines of DOWN group and NC group were counted.The expression of miR-224,API5,PAK4 and HOXD10 in QBC939 cell lines of DOWN group and NC group were measured by RT-PCR and western blotting.Results (1) MiR-224 expression was higher in CC tissues than that in normal bile duct tissues (P ＜ 0.05).(2) In QBC939 cells lines,miR-224 expression was lower in DOMN group than that in NC group.The clone formation of QBC939 cells in DOWN group was significantly lower than that of NC group.The apoptosis rate of QBC939 cells of DOWN group was significantly higher than that of NC group.(3) The expression of API5 and HOXD10 in QBC939 cells of DOWN group was significantly higher than that of NC group in the RT-PCR and western blotting experiments.(4) The expression of PAK4 in QBC939 cells of DOWN group was significantly lower than that of NC group in the RT-PCR and western blotting.Conclusions MiR-224 has anti-apoptotic effects and can promote the proliferation of QBC939 cells and may serve as an important regulator of QBC939 cell proliferation in vitro.There are miR-224-HOXD10-PAK4 signaling pathways in cholangiocarcinoma.