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OBJECTIVE@#To investigate the clinicopathological features of blastic plasmacytoid dendritic cell neoplasm (BPDCN).@*METHODS@#A total of 13 cases of BPDCN diagnosed in Peking University First Hospital from January 2013 to March 2022 were collected. The clinical features, histopathological characteristics, immunophenotypes and prognosis of the patients were analyzed retrospectively, and the related literatures was reviewed as well.@*RESULTS@#Among the 13 patients, 11 were male and 2 were female, with a median age of 62 years (ranging from 5 to 78 years). Among them, single organ involvement occurred in 5 cases, all of which presented with skin lesions. Two or more organs were involved in other 8 cases (single organ with bone marrow involved in 3 cases; skin, bone marrow and lymph node involved simultaneously in 3 cases; skin, bone marrow, lymph node and spleen involved simultaneously in 2 cases). Histopathologically, it was characterized by the proliferation of medium to large atypical blastic cells, which infiltrated the whole thickness of dermis. When involved, the bone marrow lesions mainly appeared in a diffuse pattern, while the lymph node structure was usually destroyed, and the red pulp of the affected spleen was diffusely invaded. Immunohistochemical staining showed that all the 13 cases were positive for CD4, CD56, and CD123 (13/13) in varying degrees. All the 9 cases expressed TCL1 (9/9). Variable expression of CD68 (KP1) (8/13), TdT (7/12), CD117 (2/6), and high Ki-67 proliferation index (40%~80%) were showed. The neoplastic cells lacked expressions of CD20, CD3, MPO, CD34, or CD30; EBER in situ hybridization were negative (0/9). After definite diagnosis, 6 cases received chemotherapy, among which 1 received adjuvant radiotherapy, and 2 received subsequent bone marrow transplantation. Another 2 cases only received maintenance treatment. The median follow-up time was 14 months (ranging from 6 to 36 months), 5 patients died of the disease (6 to 18 months), 3 patients survived (7 to 36 months up to now), and the remaining 5 patients lost follow-up.@*CONCLUSION@#BPDCN is a rare type of malignant lymphohematopoietic tumor with aggressive behavior and poor prognosis. The diagnosis should be made combining clinical features, histopathology, and immunohistochemical phenotype. Attention should be paid to differentiating BPDCN from other neoplasms with blastoid morphology or CD4+CD56+ tumors.
Subject(s)
Male , Female , Humans , Hematologic Neoplasms , Retrospective Studies , Dendritic Cells , Skin Neoplasms/pathology , Skin/pathology摘要
Background: the current research studies why it is effective using Anrederacordifolia, Psidium guajava, and Pogostemon cablin by the local community as a traditional medicine for diarrhea treatment caused by Escherichiacoli bacteria. Objectives: We compared the inhibitor effectiveness of three leaf extracts against Escherichia coli; we also identified the anti-bacterial substances contained in leaf extracts. Methods: We determined the bacterial test activity using the "agar diffusion" method and the thin layer chromatography (TLC) as qualitative analysis for determining the anti-bacterial substances contained in the extract. Results: The Pogostemon cablin leaf extract contained terpenoids, phenolic, and flavonoids compound as bacterial inhibitors, and the comparison showed that Pogostemon cablin leaf extract had the greatest bacterial inhibition power. Conclusion: The antibiotic substances found in the leaf extracts of Anredera cordifolia, Psidium guajava, and Pogostemon cablin can be used as traditional medicine. The breakthrough was evidenced by the ability to inhibit Escherichia coli bacteria. This research shows that traditional medicine has ancient knowledge used by this paper
Antecedentes: la presente investigación estudia la eficacia del uso de Anredera cordifolia, Psidium guajava y Pogostemoncablin por la comunidad local como medicina tradicional para el tratamiento de la diarrea causada por la bacteria Escherichia coli. Objetivos: Comparamos la eficacia inhibidora de los extractos de tres hojas contra Escherichia coli; también identificamos las sustancias antibacterianas contenidas en los extractos de hojas. Métodos: Determinamos la actividad de la prueba bacteriana mediante el método de "difusión en agar" y la cromatografía en capa fina (TLC) como análisis cualitativo para determinar las sustancias antibacterianas contenidas en el extracto. Resultados: el extracto de hoja de Pogostemoncablin contenía compuestos terpenoides, fenólicos y flavonoides como inhibidores bacterianos, y la comparación mostró que el extracto de hoja de Pogostemon cablin tenía el mayor poder de inhibición bacteriana. Conclusión: El contenido de sustancias antibióticas que se encuentran en el extracto de hoja de Anredera cordifolia, Psidium guajava y Pogostemoncablin puede utilizarse como medicina tradicional. Esto se evidencia por la capacidad de inhibir la bacteria Escherichiacoli. Esta investigación muestra que la medicina tradicional tiene un conocimiento antiguo utilizado por este artículo
Subject(s)
Humans , Anti-Bacterial Agents , Agar , Psidium , Escherichia coli , Pogostemon , Anti-Infective Agents摘要
OBJECTIVE：To establi sh the quality standard of Yao medicine Pileostegia tomentella，and to provide reference for its quality control. METHODS ：Totally 10 batches of P. tomentella were collected from Guangxi area. The properties of P. tomentella were observed and microscopic identification was conducted for the transverse section of its stems ，roots and powder. TLC method was established by using 7-hydroxycoumarin as reference. The contents of water ，total ash ，acid-insoluble ash and alcohol-soluble extract in P. tomentella were determined. The contents of skimmin ，7-hydroxycoumarin and 7-hydroxy-8- methoxycoumarin in P. tomentella were determined by HPLC. RESULTS ：The root and stem surface of P. tomentella possessed obvious wrinkles ，hard texture ，slight smell and bitter taste ，and there were secretory cells and needle crystal cells in the cross section. The medicinal powder contained calcium oxalate needle crystal ，calcium oxalate square crystal ，sclerotic cell and starch granules. The results of TLC showed that the spots of the same color were found in the corresponding positions of chromatograms of test samples and 7-hydroxycoumarin control. In the 10 batch of P. tomentella ，the contents of water were 9.1％-11.8％；those of total ash were 3.6％-6.7％；those of acid-insoluble ash were 0.10％-0.44％；those of alcohol-soluble extract were 8.9％-14.0％.The linear range of skimmin ，7-hydroxycoumarin and 7-hydroxy-8-methoxycoumarin were 6.088-182.640，1.568-47.040 and 1.096- 32.880 μg/mL（all r not less than 0.999 7）. The average recoveries were 99.47％，101.07％ and 99.89％（RSD were all less than 2％）. RSDs of precision ，stability（24 h），reproducibility and durability tests were all lower than 2％ . The contents of 3 components such as skimmin were 1.337-9.534，0.348-2.236， 0.083-1.088 mg/g，respectively. CONCLUSIONS ：It is preli- 201705） minarily proposed that the content of water in P. tomentella is not more than 12.0％ ；that of total ash is not more than 7.0％；that of alcohol-soluble extract is not less than 8.0％； E-mail：luguoshou@foxmail.com that of ski mmin is not less than 1.30 mg/g；that of 7-hydroxy- coumarin is not less than 0.30 mg/g；that of 7-hydroxy-8-methoxycoumarin is not less than 0.06 mg/g.
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Objective· To identify the effect and potential mechanism of gambogic acid (GA) on natural killer/T-cell lymphoma (NK/TCL) cell lines.Methods · SNK-1,SNK-6 and SNT-8 were incubated with various concentrations of GA for 24 h,and cell viability was detected with CCK-8 assay.Cell apoptosis was examined by Annexin V-FITC/PI staining assay.Levels of proteins regulating cell apoptosis and phosphorylation levels of proteins in key signaling pathways were detected by Western blotting.Results · GA showed a potent effect on reduction of cell viability of NK/fCL cell lines in CCK-8 assay.GA increased the percentages of Annexin V positive cells and induced activation of caspase-3 and caspase-9,cleavage of PARP as well as the reduction of Bcl-xl.GA also inhibited the phosphorylation levels of STAT3 in SNK-1 and SNT-8,and ERK1/2 in SNK-1 and SNK-6 significantly.Conclusion· GA induces cell apoptosis in NK/TCL cell lines SNK-1,SNK-6 and SNT-8.Anti-apoptosis protein Bcl-xl and signaling pathway JAKs/STATs and MEK/MAPK might be involved in this process.
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To investigate kinetics of free 177Lu and 177Lu-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION), suspensions were intravenously injected into the tail vein of mice at a dose of 5 microCi/mouse or 15 mg/kg body weight, respectively. Free 177Lu radioactivity levels were highest in kidney followed by liver and lung 1 day post-injection. 177Lu-labeled TCL-SPION radioactivity in liver and spleen was significantly higher compared to that of other organs throughout the experimental period (p < 0.05). Radioactivity in blood, brain, and epididymis rapidly declined until 28 days. Based on these results, TCL-SPION could be a safe carrier of therapeutics.
Subject(s)
Animals , Male , Mice , Body Weight , Brain , Epididymis , Iron , Kidney , Kinetics , Liver , Lung , Mice, Inbred ICR , Nanoparticles , Radioactivity , Spleen , Suspensions , Veins摘要
T-cell prolymphocytic leukemia (T-PLL) is a rare mature post-thymic T-cell malignancy with infiltration to the blood, bone marrow, lymph node, liver, spleen and skin; this disease has a poor prognosis and an aggressive clinical course. We report here on a case of CD56+ T-PLL that was diagnosed by hematological examination, immunophenotyping and molecular studies including determining the TCL1 expression by using reverse-transcriptase polymerase chain reaction (RT-PCR), and direct sequencing of the RT-PCR product.
Subject(s)
Bone Marrow , Immunophenotyping , Leukemia, Prolymphocytic, T-Cell , Liver , Lymph Nodes , Oncogenes , Polymerase Chain Reaction , Prognosis , Skin , Spleen , T-Lymphocytes摘要
OBJECTIVE:To establish the quality standard of Sophora flavescens decoction.METHODS: The preparation was determined qualitatively using TLC,and the contents of matrine and oxymatrine were determined by HPLC.RESULTS: The TLC spots of S.flavescens decoction were clear and well-separated.The linear ranges of matrine and oxymatrine were 0.024 92～0.648 44 ?g(r=0.999 9) and 0.073 4～1.908 4 ?g(r=0.999 9).The average recoveries were 102.9% and 102.3% and RSDs were 6.9% and 102.3%,respectively (n=6).CONCLUSION: Establish standard can be used for the quality control of S.flavescens decoction.
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Objective: To assess the therapeutic effect and safety of Gongmo Kang capsule (GKC) in treating metrorrhagia (with blood stasis syndrome) caused by drug - induced abortion. Methods: A randomized double - blind controlled design was adopted. One hundred and five patients were treated with GKC, and other 104 patients in the control group with Gongxue Ning capsule (GNC). Results: In GKC group, the remarkably effective rate was 73. 33%, the total effective rate was 85. 70% ; the remarkably effective rate for improving syndromes was 80.00% and the total effective rate was 88.57% ; the mean bleeding period was (4.46 ? 1.71) days. GKC appeared to be more effective than GNC (P
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AIM: The quality standard for Compound Xiaojingtong Capsule(Radix Astragali, Radix Ginseng, Radix Angelicae Sinensis, Rhizoma Chuanxiong, etc.) were studied. METHODS: The TLC method for identification of Radix Ginseng, Radix Angelicae Sinensis, Rhizoma Chuanxiong in these capsules were established. Astragaloside Ⅳ was determined by single wavelength TLC-scanning(? s=530nm). RESULTS: Radix Angelicae Sinensis, Rhizoma Chuanxiong and Radix Ginseng could be detected. Astragaloside Ⅳ showed a linear relationship at the concentration range of 1.1～5.5?g (r=0.9983). The average recovery was 100.1% and RSD was 1.59% (n=6). CONCLUSION: This method is suitable for the quality control of Compound Xiaojingtong Capsule.
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AIM: To establish the method for quality control of Guipi Pills(concentrated pill) (Radix Codonopsis,Rhizoma Atractylodis macrocephalae,Radix Astragali,Radix et Rhizoma Glycyrrhizae,etc).and a quantitative method of determining astragaloside IV. METHODS: Radix Codonopsis,Radix Angelicae Sinensis,Radix et Rhizoma Glycyrrhizae and Radix Aucklandiae in the pill were identified by TLC.Astragaloside IV in the pill was determined by HPLC-ELSD. RESULTS: For astragaloside IV,the linear range was within 25.4-508.3 ?g and the average recovery was 99.1%,r=0.999 7 with RSD of 0.8%. CONCLUSION: The method is simple,reliable,accurate,practical.It can be used in the quality control of Gui Pipills(concentrated pills).
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AIM: To establish a quality standard for Kudingcha Tablets(Cratoxylum Prunifolium Dyer). METHODS: TLC was adopted for identification of Kudingcha Tablets.RP-HPLC was used to determine the contents of ursolic acid,quercetin and kaempferol in the preparation.The Nova-Pak C_(18) column(3.9 mm?150 mm,4.0 ?m) was used to determine the ursolic acid of content.The mobile phase was methanol-water-H_3PO_4(85∶15∶0.1).The detection wavelength was at 220 nm.The flow rate was 0.8 mL/min and the temperature was at 30 ℃.The Nova-Pak C_(18) column(3.9 mm?300 mm,4.0 ?m) was used to determine the contents of quercetin and kaempferol.The mobile phase was methanol-0.4%H_3PO_4(50:50).The detection wavelength was at 360 nm.The flow rate was 0.7 mL/min.The temperature was at 40 ℃. RESULTS: A good linear relation was obtained when the sample size of ursolic acid was in the range of 0.334-6.672 ?g.The linear range of quercetin was(0.012 99-0.649 6) ?g and the linear range of kaempferol was 0.013 34-0.667 2 ?g.The average recoveries of ursolic acid,quercetin and kaempferol were 97.70%,96.89% and 97.41%,respectively.The RSD were 0.87%,(1.06%) and 1.40%(n=6),respectively. CONCLUSION: This standard is accurate and reliable,and can effectively control the quality of Kudingcha Tablets.