摘要
Objective To study the effect of Pirin(an iron-binding nuclear protein)on the proliferation and self-renewal of glioma stem cell(GSC)so as to provide a potential therapy target for malignant glioma.Methods PLKO.1-shPirin lentiviral plasmids were constructed to stably knock down Pirin in GSC by using lentivirus infection system.The interference efficiency of Pirin short hair?pin RNA(shRNA)was detected by Western blotting. The capacity of GSC was examined by the assessment of cell viability. Tumor sphere formation assay was used to detect the effect of Pirin on GSC self-renewal capacity. Results Pirin was highly expressed in GSC.Consistently,the protein level of Pirin in the conditioned medium from GSC was much higher than that from the corresponding non-stem tumor cell(NSTC).Gene-sequencing analysis demonstrated that PLKO.1-shPirin lentiviral plasmids were successfully con?structed.Pirin shRNA transfection significantly inhibited the expression of Pirin in GSC and suppressed the cell viability and ability of tumorsphere formation.Conclusion Knocking down Pirin significantly inhibites the cell proliferation and self-renewal of GSC.
摘要
@#Objective To study the effect of hyperbaric oxygen(HBO)therapy on abilities of learning and memory and hippocampal pyramidal cell in vascular dementia rats.MethodsThe vascular dementia model was reproduced and divided into control,sham and treatment groups.After 30 d of HBO therapy,the rats were assessed with the Morris water maze,and their hippocampal pyramidal cells were observed after HE staining.ResultsCompared with the control group,the ability of learning and memory of rats in treatment group increased,as well as the hippocampal pyramidal cells did.ConclusionHyperbaric oxygen therapy can be used to treat vascular dementia.