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Effect of Bitis gabonica and Dendroaspis angusticeps snake venoms on apoptosis-related genes in human thymic epithelial cells
Boda, Francisc; Banfai, Krisztina; Garai, Kitti; Kovacs, Bela; Almasi, Attila; Scheffer, Dalma; Sinkler, Reka Lambertne; Csonka, Robert; Czompoly, Tamas; Kvell, Krisztian.
Affiliation
  • Boda, Francisc; University of Medicine, Pharmacy, Science, and Technology of Targu Mures. Faculty of Pharmacy. Targu Mures. RO
  • Banfai, Krisztina; University of Pecs. Faculty of Pharmacy. Department of Pharmaceutical Biotechnology. Pecs. HU
  • Garai, Kitti; University of Pecs. Faculty of Pharmacy. Department of Pharmaceutical Biotechnology. Pecs. HU
  • Kovacs, Bela; University of Medicine, Pharmacy, Science, and Technology of Targu Mures. Faculty of Pharmacy. Department F1. Targu Mures. RO
  • Almasi, Attila; University of Pecs. Faculty of Pharmacy. Department of Pharmaceutical Chemistry. Pecs. HU
  • Scheffer, Dalma; University of Pecs. Szentagothai Research Center. Food Biotechnology Research Group. Pecs. HU
  • Sinkler, Reka Lambertne; University of Pecs. Szentagothai Research Center. Food Biotechnology Research Group. Pecs. HU
  • Csonka, Robert; University of Pecs. Szentagothai Research Center. Food Biotechnology Research Group. Pecs. HU
  • Czompoly, Tamas; University of Pecs. Szentagothai Research Center. Food Biotechnology Research Group. Pecs. HU
  • Kvell, Krisztian; University of Pecs. Faculty of Pharmacy. Department of Pharmaceutical Biotechnology. Pecs. HU
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;26: e20200057, 2020. tab, graf
Article ي En | LILACS, VETINDEX | ID: biblio-1143218
المكتبة المسؤولة: BR68.1
ABSTRACT
Certain environmental toxins permanently damage the thymic epithelium, accelerate immune senescence and trigger secondary immune pathologies. However, the exact underlying cellular mechanisms and pathways of permanent immune intoxication remain unknown. The aim of the present study was to demonstrate gene expressional changes of apoptosis-related cellular pathways in human thymic epithelial cells following exposure to snake venom from Bitis gabonica and Dendroaspis angusticeps.

Methods:

Snake venoms were characterized by analytical methods including reversed phase high-performance liquid chromatography and sodium dodecyl sulphate-polyacrylamide gel electrophoresis, then applied on human thymic epithelial cells (1889c) for 24 h at 10 μg/mL (as used in previous TaqMan Array study). Gene expressional changes restricted to apoptosis were assayed by TaqMan Array (Human Apoptosis Plate).

Results:

The most prominent gene expressional changes were shown by CASP5 (≈ 2.5 million-fold, confirmed by dedicated quantitative polymerase chain reaction) and CARD9 (0.016-fold) for B. gabonica, and BIRC7 (6.46-fold) and CASP1 (0.30-fold) for D. angusticeps.

Conclusion:

The observed apoptotic environment suggests that pyroptosis may be the dominant pathway through which B. gabonica and D. angusticeps snake venoms trigger thymic epithelial apoptosis following envenomation.(AU)
الموضوعات
Key words

النص الكامل: 1 الفهرس: LILACS الموضوع الرئيسي: Snake Venoms / Polymerase Chain Reaction / Apoptosis / Viperidae / Epithelial Cells / Pyroptosis المحددات: Animals اللغة: En مجلة: J. venom. anim. toxins incl. trop. dis موضوع المجلة: TOXICOLOGIA السنة: 2020 نوع: Article

النص الكامل: 1 الفهرس: LILACS الموضوع الرئيسي: Snake Venoms / Polymerase Chain Reaction / Apoptosis / Viperidae / Epithelial Cells / Pyroptosis المحددات: Animals اللغة: En مجلة: J. venom. anim. toxins incl. trop. dis موضوع المجلة: TOXICOLOGIA السنة: 2020 نوع: Article